Restriction map for pF1KB7139
 (Linear) (Six-base) MAPSORT of: KIBB7139.seq  Check: 2022  from: 1  to: 999



KIBB7139.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     698     999 
   Size:        698     301
 
AccI GT'mk_AC

Cuts at:      0     272     999 
   Size:        272     727
 
AhdI GACnn_n'nnGTC

Cuts at:      0     144     999 
   Size:        144     855
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     505     999 
   Size:        505     494
 
ApaI G_GGCC'C

Cuts at:      0     818     999 
   Size:        818     181
 
ApaLI G'TGCA_C

Cuts at:      0     685     999 
   Size:        685     314
 
BanI G'GyrC_C

Cuts at:      0     457     521     757     999 
   Size:        457      64     236     242

  Fragments arranged by size: 

                 457     242     236      64
 
BanII G_rGCy'C

Cuts at:      0     548     818     851     999 
   Size:        548     270      33     148

  Fragments arranged by size: 

                 548     270     148      33
 
BbeI G_GCGC'C

Cuts at:      0     525     999 
   Size:        525     474
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     622     780     896     999 
   Size:        622     158     116     103

  Fragments arranged by size: 

                 622     158     116     103
 
BbvCI CC'TCA_GC

Cuts at:      0     706     999 
   Size:        706     293
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     447     481     554     588     999 
   Size:        447      34      73      34     411

  Fragments arranged by size: 

                 447     411      73      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     119     530     999 
   Size:        119     411     469

  Fragments arranged by size: 

                 469     411     119
 
BglII A'GATC_T

Cuts at:      0      68     407     999 
   Size:         68     339     592

  Fragments arranged by size: 

                 592     339      68
 
Bme1580I G_kGCm'C

Cuts at:      0     345     460     689     726     760     818     999 
   Size:        345     115     229      37      34      58     181

  Fragments arranged by size: 

                 345     229     181     115      58      37      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      18     240     879     999 
   Size:         18     222     639     120

  Fragments arranged by size: 

                 639     222     120      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     697     999 
   Size:         40     657     302

  Fragments arranged by size: 

                 657     302      40
 
Bpu10I CC'TnA_GC

Cuts at:      0     706     999 
   Size:        706     293
 
BsaHI Gr'CG_yC

Cuts at:      0     522     901     999 
   Size:        522     379      98

  Fragments arranged by size: 

                 522     379      98
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     535     565     999 
   Size:        535      30     434

  Fragments arranged by size: 

                 535     434      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     753     904     999 
   Size:        753     151      95

  Fragments arranged by size: 

                 753     151      95
 
BseYI C'CCAG_C

Cuts at:      0     170     967     999 
   Size:        170     797      32

  Fragments arranged by size: 

                 797     170      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     165     999 
   Size:        165     834
 
BsiEI CG_ry'CG

Cuts at:      0      56     386     999 
   Size:         56     330     613

  Fragments arranged by size: 

                 613     330      56
 
BsiHKAI G_wGCw'C

Cuts at:      0      50      86     450     479     570     689     999 
   Size:         50      36     364      29      91     119     310

  Fragments arranged by size: 

                 364     310     119      91      50      36      29
 
Bsp1286I G_dGCh'C

Cuts at:      0      50      86     345     450     460     479     548     570 
   Size:         50      36     259     105      10      19      69      22

Cuts at:    570     689     726     760     818     851     999 
   Size:        119      37      34      58      33     148

  Fragments arranged by size: 

                 259     148     119     105      69      58      50      37
                  36      34      33      22      19      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     680     698     999 
   Size:        680      18     301

  Fragments arranged by size: 

                 680     301      18
 
BsrBI CCG'CTC

Cuts at:      0     312     372     999 
   Size:        312      60     627

  Fragments arranged by size: 

                 627     312      60
 
BsrFI r'CCGG_y

Cuts at:      0     118     348     999 
   Size:        118     230     651

  Fragments arranged by size: 

                 651     230     118
 
BssSI C'ACGA_G

Cuts at:      0     583     999 
   Size:        583     416
 
BstYI r'GATC_y

Cuts at:      0      68     407     999 
   Size:         68     339     592

  Fragments arranged by size: 

                 592     339      68
 
BtgI C'CryG_G

Cuts at:      0      56     441     539     608     803     999 
   Size:         56     385      98      69     195     196

  Fragments arranged by size: 

                 385     196     195      98      69      56
 
BtsI GCAGTG_nn'

Cuts at:      0     343     999 
   Size:        343     656
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     595     999 
   Size:        595     404
 
EaeI y'GGCC_r

Cuts at:      0     116     350     383     401     513     945     999 
   Size:        116     234      33      18     112     432      54

  Fragments arranged by size: 

                 432     234     116     112      54      33      18
 
EagI C'GGCC_G

Cuts at:      0     383     999 
   Size:        383     616
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     253     302     925     999 
   Size:         91     162      49     623      74

  Fragments arranged by size: 

                 623     162      91      74      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     697     999 
   Size:         40     657     302

  Fragments arranged by size: 

                 657     302      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0     357     814     815     911     999 
   Size:        357     457       1      96      88

  Fragments arranged by size: 

                 457     357      96      88       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     639     999 
   Size:        639     360
 
FspI TGC'GCA

Cuts at:      0     436     999 
   Size:        436     563
 
HaeII r_GCGC'y

Cuts at:      0     379     525     999 
   Size:        379     146     474

  Fragments arranged by size: 

                 474     379     146
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     999 
   Size:        130      32     837

  Fragments arranged by size: 

                 837     130      32
 
KasI G'GCGC_C

Cuts at:      0     521     999 
   Size:        521     478
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     627     999 
   Size:        627     372
 
MscI TGG'CCA

Cuts at:      0     403     515     947     999 
   Size:        403     112     432      52

  Fragments arranged by size: 

                 432     403     112      52
 
MslI CAynn'nnrTG

Cuts at:      0     634     640     999 
   Size:        634       6     359

  Fragments arranged by size: 

                 634     359       6
 
MspA1I CmG'CkG

Cuts at:      0     443     999 
   Size:        443     556
 
NaeI GCC'GGC

Cuts at:      0     120     350     999 
   Size:        120     230     649

  Fragments arranged by size: 

                 649     230     120
 
NarI GG'CG_CC

Cuts at:      0     522     999 
   Size:        522     477
 
NcoI C'CATG_G

Cuts at:      0     539     999 
   Size:        539     460
 
NgoMIV G'CCGG_C

Cuts at:      0     118     348     999 
   Size:        118     230     651

  Fragments arranged by size: 

                 651     230     118
 
PasI CC'CwG_GG

Cuts at:      0     753     999 
   Size:        753     246
 
PfoI T'CCnGG_A

Cuts at:      0      17      41     923     999 
   Size:         17      24     882      76

  Fragments arranged by size: 

                 882      76      24      17
 
PpuMI rG'GwC_Cy

Cuts at:      0     357     999 
   Size:        357     642
 
PspOMI G'GGCC_C

Cuts at:      0     814     999 
   Size:        814     185
 
PstI C_TGCA'G

Cuts at:      0     242     999 
   Size:        242     757
 
SacII CC_GC'GG

Cuts at:      0     444     999 
   Size:        444     555
 
SapI GCTCTTCn'nnn_

Cuts at:      0      91     302     999 
   Size:         91     211     697

  Fragments arranged by size: 

                 697     211      91
 
SexAI A'CCwGG_T

Cuts at:      0      35     782     999 
   Size:         35     747     217

  Fragments arranged by size: 

                 747     217      35
 
SfcI C'TryA_G

Cuts at:      0     238     999 
   Size:        238     761
 
SfoI GGC'GCC

Cuts at:      0     523     999 
   Size:        523     476
 
StuI AGG'CCT

Cuts at:      0     205     999 
   Size:        205     794
 
StyI C'CwwG_G

Cuts at:      0     353     539     999 
   Size:        353     186     460

  Fragments arranged by size: 

                 460     353     186
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     222     276     999 
   Size:        222      54     723

  Fragments arranged by size: 

                 723     222      54
 
Tth111I GACn'n_nGTC

Cuts at:      0      37     784     999 
   Size:         37     747     215

  Fragments arranged by size: 

                 747     215      37
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     411     999 
   Size:        411     588
 

 Enzymes that do cut:

     AarI     AccI     AhdI     AlfI     ApaI    ApaLI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BglI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI    BstYI     BtgI
     BtsI     DrdI     EaeI     EagI     EarI  Eco57MI EcoO109I     FalI
     FspI    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     PasI     PfoI    PpuMI   PspOMI
     PstI    SacII     SapI    SexAI     SfcI     SfoI     StuI     StyI
    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AleI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI     XbaI     XhoI     XmaI     XmnI
     ZraI
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