Restriction map for pF1KB7139
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AlwI        AlwNI       
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AvaI        AvrII       
BamHI       BbrPI       BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tsp509I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          271  GTCTAC               GT^[AC][GT]AC
AccII         442  CGCG                 CG^CG
AfaI          528  GTAC                 GT^AC
AhdI          139  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        685  GTGCAC               G^TGCAC
ApaI          814  GGGCCC               GGGCC^C
ApaLI         685  GTGCAC               G^TGCAC
AspEI         139  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         434  TGCGCA               TGC^GCA
BbeI          521  GGCGCC               GGCGC^C
BbvCI         705  CCTCAGC              CCTCAGC(-5/-2)
BcnI          711  CCGGG                CC^[GC]GG
BfaI          493  CTAG                 C^TAG
BsgI          180  CTGCAC               GTGCAG(16/14)
BsmFI         965  GTCCC                GGGAC(10/14)
BssSI         583  CTCGTG               CACGAG(-5/-1)
BstUI         442  CGCG                 CG^CG
DrdI          589  GACTGCTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          383  CGGCCG               C^GGCCG
Eam1105I      139  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         383  CGGCCG               C^GGCCG
Eco52I        383  CGGCCG               C^GGCCG
FspI          434  TGCGCA               TGC^GCA
KasI          521  GGCGCC               G^GCGCC
KspI          441  CCGCGG               CCGC^GG
MaeI          493  CTAG                 C^TAG
MaeII         188  ACGT                 A^CGT
MseI          668  TTAA                 T^TAA
MspA1I        441  CCGCGG               C[AC]G^C[GT]G
MvnI          442  CGCG                 CG^CG
NarI          521  GGCGCC               GG^CGCC
NciI          711  CCGGG                CC^[GC]GG
NcoI          539  CCATGG               C^CATGG
PpuMI         356  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          238  CTGCAG               CTGCA^G
RsaI          528  GTAC                 GT^AC
SacII         441  CCGCGG               CCGC^GG
SfcI          238  CTGCAG               C^T[GA][CT]AG
SfoI          521  GGCGCC               GGC^GCC
StuI          203  AGGCCT               AGG^CCT
Tru9I         668  TTAA                 T^TAA
XcmI          404  CCAAGATCTCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AcyI          521  GGCGCC               G[GA]^CG[CT]C
              900  GGCGTC              
AluI          335  AGCT                 AG^CT
              973  AGCT                
AspI           34  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              781  GACCAGGTC           
BcgI          565  CGAGCACCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              458  GCACCTATGTCG        
BglI          113  GCCTGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              524  GCCTGTACGGC         
BglII          68  AGATCT               A^GATCT
              407  AGATCT              
BpmI           19  CTGGAG               CTGGAG(16/14)
              676  CTGGAG              
BsaHI         521  GGCGCC               G[GA]^CG[CT]C
              900  GGCGTC              
BseRI         762  CTCCTC               GAGGAG(10/8)
              889  GAGGAG              
BsiEI          53  CGACCG               CG[GA][CT]^CG
              383  CGGCCG              
BsmAI         297  GTCTC                GTCTC(1/5)
              598  GTCTC               
BspMI         671  ACCTGC               ACCTGC(4/8)
              689  ACCTGC              
BsrBI         310  GAGCGG               CCGCTC(-3/-3)
              370  GAGCGG              
BsrFI         118  GCCGGC               [GA]^CCGG[CT]
              348  GCCGGC              
BstYI          68  AGATCT               [GA]^GATC[CT]
              407  AGATCT              
Cfr10I        118  GCCGGC               [GA]^CCGG[CT]
              348  GCCGGC              
DpnII          69  GATC                 ^GATC
              408  GATC                
EcoT14I       353  CCAAGG               C^C[AT][AT]GG
              539  CCATGG              
FauI          339  GCGGG                CCCGC(4/6)
              372  GCGGG               
HaeII         375  GGCGCT               [GA]GCGC^[CT]
              521  GGCGCC              
Hin1I         521  GGCGCC               G[GA]^CG[CT]C
              900  GGCGTC              
HinfI         124  GAGTC                G^A[ACGT]TC
              650  GACTC               
HphI            4  GGTGA                GGTGA(8/7)
              136  GGTGA               
MboI           69  GATC                 ^GATC
              408  GATC                
MflI           68  AGATCT               [GA]^GATC[CT]
              407  AGATCT              
MslI          630  CATCTTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              636  CATGTTGGTG          
NaeI          118  GCCGGC               GCC^GGC
              348  GCCGGC              
NdeII          69  GATC                 ^GATC
              408  GATC                
NgoMIV        118  GCCGGC               G^CCGGC
              348  GCCGGC              
PflFI          34  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              781  GACCAGGTC           
PleI          650  GACTC                GAGTC(4/5)
              124  GAGTC               
SapI          307  GAAGAGC              GCTCTTC(1/4)
               84  GCTCTTC             
Sau3AI         69  GATC                 ^GATC
              408  GATC                
SexAI          35  ACCAGGT              A^CC[AT]GGT
              782  ACCAGGT             
SfaNI          98  GCATC                GCATC(5/9)
              437  GCATC               
StyI          353  CCAAGG               C^C[AT][AT]GG
              539  CCATGG              
TaqI           52  TCGA                 T^CGA
              155  TCGA                
Tsp45I          5  GTGAC                ^GT[GC]AC
              137  GTGAC               
Tth111I        34  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              781  GACCAGGTC           
TthHB8I        52  TCGA                 T^CGA
              155  TCGA                
XhoII          68  AGATCT               [GA]^GATC[CT]
              407  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AspHI       AvaII       BalI        BanI        
BanII       BbsI        BbvI        BmyI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HpaII       ItaI        Ksp632I     MaeIII      
MboII       MluNI       MnlI        MscI        MspI        
MvaI        MwoI        NlaIII      NlaIV       Sau96I      
ScrFI       TseI        TspRI       
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