C8B ORF clone FHC31629

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Halotag ORF Clone FHC31629

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Clone info.

Species

human

Clone Name

pFN21AE2013 Sequence (with vector) Restriction Map

Product ID

FHC31629

ORF Sequence

DNA sequence (1773 bp)
Protein sequence (591 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB591028]

Gene

KIEE2013

Symbol

C8B

Alias : C82

Description

complement component 8, beta polypeptide, transcript variant 1

Original Clone ID

cs02913

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1773 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 591 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS30730.1 5.5e-207 99.8 C8B CCDS60152.1 2.2e-188 99.8 C8B CCDS60151.1 1.5e-176 99.6 C8B CCDS3936.1 2.2e-49 31.0 C6 CCDS606.1 4.3e-38 33.6 C8A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001862 84 100 PR00764 Membrane attack complex component/perforin/complement C9 IPR001862 126 146 PR00764 Membrane attack complex component/perforin/complement C9 IPR001862 322 344 PR00764 Membrane attack complex component/perforin/complement C9 IPR001862 451 470 PR00764 Membrane attack complex component/perforin/complement C9 IPR001862 482 501 PR00764 Membrane attack complex component/perforin/complement C9 IPR001862 505 525 PR00764 Membrane attack complex component/perforin/complement C9 HMMPfam IPR002172 123 155 PF00057 Low-density lipoprotein (LDL) receptor class A repeat IPR020864 283 495 PF01823 Membrane attack complex component/perforin (MACPF) domain HMMSmart IPR000884 67 117 SM00209 Thrombospondin IPR002172 121 157 SM00192 Low-density lipoprotein (LDL) receptor class A repeat IPR020864 291 498 SM00457 Membrane attack complex component/perforin (MACPF) domain IPR000884 548 591 SM00209 Thrombospondin ProfileScan IPR000884 64 117 PS50092 Thrombospondin IPR002172 121 156 PS50068 Low-density lipoprotein (LDL) receptor class A repeat IPR020864 158 504 PS51412 Membrane attack complex component/perforin (MACPF) domain IPR000884 545 591 PS50092 Thrombospondin
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000057 591 120960,613789 100.0 100.0 XP_016857724 591 120960,613789 99.8 100.0 NP_001265472 539 120960,613789 100.0 91.2 NP_001265473 529 120960,613789 99.8 86.1 NP_001108603 934 217050,612446 31.0 89.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2013 Download

>KIEE2013  1773 bp
ATGAAGAATTCCAGGACATGGGCTTGGAGGGCGCCGGTGGAGCTATTTCTTCTCTGTGCT
GCCCTGGGCTGTCTCAGTTTGCCTGGCTCCAGAGGTGAAAGGCCACATTCCTTTGGGTCA
AATGCAGTCAACAAGAGCTTTGCTAAGAGCAGACAGATGCGGAGTGTGGATGTTACCCTG
ATGCCCATTGATTGTGAGCTGTCTAGTTGGTCCTCTTGGACCACATGTGACCCCTGTCAG
AAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGC
AACTTCTCTGACAAGGAAGTCGAAGACTGTGTTACCAACAGACCATGCAGAAGTCAAGTG
CGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGC
AATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAA
AAATGTCAGCATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGTGGGATAAAT
TTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGC
TCCCCGCATTACATCCTGAACACGAGGTTTAGGAAGCCCTACAATGTGGAAAGCTACACG
CCACAGACCCAAGGCAAATACGAATTCATATTAAAAGAGTATGAATCATACTCAGATTTT
GAACGCAATGTCACAGAGAAAATGGCAAGCAAGTCTGGTTTCAGTTTTGGTTTTAAAATA
CCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGTGATCGAGGCAAACACTATATTAGG
AGAACCAAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCACGCTCTGACCTTGAA
GTAGCACATTACAAGCTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCAGAGA
GTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGAATACAGAGATCTCTTCCGTGATTTT
GGGACCCACTACATCACAGAGGCTGTGCTTGGGGGCATTTATGAATACACCCTCGTTATG
AACAAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACAACGTCCATGCCTGTGCCAAA
AATGATTTTAAAATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGGTGTGTCTGTA
GGCAAATGCAGAGGTATTCTGAATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTG
GAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCACCACCCTGGCATAC
CAGGAGCTGCCGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAACCCA
GCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCC
TATTCCAGCACAGTGAGGCAGAACATGAAGCAGGCACTGGAGGAGTTCCAGAAGGAAGTT
AGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCCCTGTCCTGAAAGGATCACGC
TGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTCCTATCGGAAG
AATACCCCCATTGATGGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGA
CGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGT
TCAGGCCCTGCTTCAGAAACACTTGACTGCTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE2013 Download

>KIEE2013  591 aa
MKNSRTWAWRAPVELFLLCAALGCLSLPGSRGERPHSFGSNAVNKSFAKSRQMRSVDVTL
MPIDCELSSWSSWTTCDPCQKKRYRYAYLLQPSQFHGEPCNFSDKEVEDCVTNRPCRSQV
RCEGFVCAQTGRCVNRRLLCNGDNDCGDQSDEANCRRIYKKCQHEMDQYWGIGSLASGIN
LFTNSFEGPVLDHRYYAGGCSPHYILNTRFRKPYNVESYTPQTQGKYEFILKEYESYSDF
ERNVTEKMASKSGFSFGFKIPGIFELGISSQSDRGKHYIRRTKRFSHTKSVFLHARSDLE
VAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFRDFGTHYITEAVLGGIYEYTLVM
NKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGILNEIKDRNKRDTMV
EDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTATDFA
YSSTVRQNMKQALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRK
NTPIDGKWNCWSNWSSCSGRRKTRQRQCNNPPPQNGGSPCSGPASETLDCS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2013 Download

>pFN21AE2013  6478 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGAATTCCAGGACATGGGCTTGGAGGGCGCC
GGTGGAGCTATTTCTTCTCTGTGCTGCCCTGGGCTGTCTCAGTTTGCCTGGCTCCAGAGG
TGAAAGGCCACATTCCTTTGGGTCAAATGCAGTCAACAAGAGCTTTGCTAAGAGCAGACA
GATGCGGAGTGTGGATGTTACCCTGATGCCCATTGATTGTGAGCTGTCTAGTTGGTCCTC
TTGGACCACATGTGACCCCTGTCAGAAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCC
CTCTCAGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAAGACTGTGTTAC
CAACAGACCATGCAGAAGTCAAGTGCGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAG
GTGTGTAAACCGCAGACTTCTTTGCAATGGGGACAATGACTGTGGAGACCAGTCAGATGA
AGCAAACTGTAGAAGGATTTATAAAAAATGTCAGCATGAAATGGACCAATACTGGGGAAT
TGGCAGTCTGGCCAGTGGGATAAATTTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGA
TCACAGGTATTATGCAGGTGGATGCTCCCCGCATTACATCCTGAACACGAGGTTTAGGAA
GCCCTACAATGTGGAAAGCTACACGCCACAGACCCAAGGCAAATACGAATTCATATTAAA
AGAGTATGAATCATACTCAGATTTTGAACGCAATGTCACAGAGAAAATGGCAAGCAAGTC
TGGTTTCAGTTTTGGTTTTAAAATACCTGGAATATTTGAACTTGGCATCAGTAGTCAAAG
TGATCGAGGCAAACACTATATTAGGAGAACCAAACGATTCTCTCATACTAAAAGCGTATT
TCTGCATGCACGCTCTGACCTTGAAGTAGCACATTACAAGCTGAAACCCAGAAGCCTCAT
GCTCCATTACGAGTTCCTTCAGAGAGTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGA
ATACAGAGATCTCTTCCGTGATTTTGGGACCCACTACATCACAGAGGCTGTGCTTGGGGG
CATTTATGAATACACCCTCGTTATGAACAAAGAGGCCATGGAGAGAGGAGATTATACTCT
TAACAACGTCCATGCCTGTGCCAAAAATGATTTTAAAATTGGTGGTGCCATTGAAGAGGT
CTACGTCAGTCTGGGTGTGTCTGTAGGCAAATGCAGAGGTATTCTGAATGAAATAAAAGA
CAGAAACAAGAGGGACACCATGGTGGAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAG
TGAGCACATCACCACCCTGGCATACCAGGAGCTGCCGACGGCGGACCTGATGCAGGAGTG
GGGAGACGCTGTGCAGTACAACCCAGCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGA
ACTAGTGACAGCCACAGATTTTGCCTATTCCAGCACAGTGAGGCAGAACATGAAGCAGGC
ACTGGAGGAGTTCCAGAAGGAAGTTAGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGG
AGTCCCTGTCCTGAAAGGATCACGCTGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCT
AGCCTGTGAGGTCTCCTATCGGAAGAATACCCCCATTGATGGGAAGTGGAATTGCTGGTC
AAATTGGTCTTCATGCTCTGGAAGACGTAAGACAAGACAAAGGCAGTGTAACAATCCACC
TCCTCAAAATGGGGGTAGCCCCTGTTCAGGCCCTGCTTCAGAAACACTTGACTGCTCCGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT