(Circular) (Six-base) MAPSORT of: pFN21AE2013.seq Check: 815 from: 1 to: 6478 pFN21AE2013 6478 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2585 2585 Size: 6478 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4288 278 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 3821 1937 Size: 1243 641 4594 Fragments arranged by size: 4594 1243 641 Acc65I G'GTAC_C Cuts at: 3799 3799 Size: 6478 AclI AA'CG_TT Cuts at: 1563 4605 4978 1563 Size: 3042 373 3063 Fragments arranged by size: 3063 3042 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2942 3741 4541 5925 6193 1632 Size: 1310 799 800 1384 268 1917 Fragments arranged by size: 1917 1384 1310 800 799 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6281 AflIII A'CryG_T Cuts at: 1203 2228 5377 6354 1203 Size: 1025 3149 977 1327 Fragments arranged by size: 3149 1327 1025 977 AgeI A'CCGG_T Cuts at: 5309 5309 Size: 6478 AhdI GACnn_n'nnGTC Cuts at: 5207 5207 Size: 6478 AleI CACnn'nnGTG Cuts at: 1166 3260 1166 Size: 2094 4384 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2177 2617 1184 Size: 993 440 5045 Fragments arranged by size: 5045 993 440 AlwNI CAG_nnn'CTG Cuts at: 3047 3331 3481 3752 4221 5793 3047 Size: 284 150 271 469 1572 3732 Fragments arranged by size: 3732 1572 469 284 271 150 ApaI G_GGCC'C Cuts at: 2570 2570 Size: 6478 ApaLI G'TGCA_C Cuts at: 2385 4535 5691 2385 Size: 2150 1156 3172 Fragments arranged by size: 3172 2150 1156 ApoI r'AATT_y Cuts at: 1493 2011 2542 2687 3787 4013 4165 6274 1493 Size: 518 531 145 1100 226 152 2109 1697 Fragments arranged by size: 2109 1697 1100 531 518 226 152 145 AseI AT'TA_AT Cuts at: 160 1032 5032 160 Size: 872 4000 1606 Fragments arranged by size: 4000 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 AvaI C'yCGr_G Cuts at: 1870 1943 3803 1870 Size: 73 1860 4545 Fragments arranged by size: 4545 1860 73 BamHI G'GATC_C Cuts at: 3587 3808 4175 3587 Size: 221 367 5890 Fragments arranged by size: 5890 367 221 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 3164 3799 5254 618 Size: 325 217 627 248 1129 635 1455 1842 Fragments arranged by size: 1842 1455 1129 635 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2570 3797 1391 Size: 1179 1227 4072 Fragments arranged by size: 4072 1227 1179 BbeI G_GCGC'C Cuts at: 2039 2039 Size: 6478 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2334 3660 3688 928 Size: 971 435 1326 28 3718 Fragments arranged by size: 3718 1326 971 435 28 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4669 4703 1903 Size: 34 2732 34 3678 Fragments arranged by size: 3678 2732 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4370 5580 4370 Size: 1210 5268 BclI T'GATC_A Cuts at: 1548 2578 1548 Size: 1030 5448 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5089 136 Size: 107 122 71 631 605 3417 1525 Fragments arranged by size: 3417 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3007 6473 3007 Size: 3466 3012 BlpI GC'TnA_GC Cuts at: 3895 3895 Size: 6478 Bme1580I G_kGCm'C Cuts at: 1790 2389 2570 4539 5695 1790 Size: 599 181 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 599 181 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6478 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2521 2564 4209 Size: 416 53 83 399 24 1104 43 1645 Cuts at: 4209 5167 442 Size: 958 1753 Fragments arranged by size: 1753 1645 1104 958 416 399 83 53 43 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2258 3002 3503 5138 1239 Size: 838 181 744 501 1635 2579 Fragments arranged by size: 2579 1635 838 744 501 181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4603 5468 5766 4603 Size: 865 298 5315 Fragments arranged by size: 5315 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 3616 5141 882 Size: 617 940 1177 1525 2219 Fragments arranged by size: 2219 1525 1177 940 617 BsaAI yAC'GTr Cuts at: 493 1204 4269 493 Size: 711 3065 2702 Fragments arranged by size: 3065 2702 711 BsaBI GATnn'nnATC Cuts at: 6472 6472 Size: 6478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 4285 4667 5302 Size: 53 83 186 936 503 2249 382 635 Cuts at: 5302 275 Size: 1451 Fragments arranged by size: 2249 1451 936 635 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4911 5309 5583 5730 6141 4911 Size: 398 274 147 411 5248 Fragments arranged by size: 5248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3354 3384 3398 3428 3531 Size: 30 165 30 1951 30 14 30 103 Cuts at: 3531 3561 1178 Size: 30 4095 Fragments arranged by size: 4095 1951 165 103 30 30 30 30 30 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3120 3303 3500 3711 1186 Size: 1934 183 197 211 3953 Fragments arranged by size: 3953 1934 211 197 183 BseYI C'CCAG_C Cuts at: 3382 5681 6177 3382 Size: 2299 496 3683 Fragments arranged by size: 3683 2299 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3392 1163 Size: 573 1656 4249 Fragments arranged by size: 4249 1656 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3928 4689 5717 1540 Size: 275 186 1927 761 1028 2301 Fragments arranged by size: 2301 1927 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2389 3306 3523 4539 4624 5695 2389 Size: 917 217 1016 85 1071 3172 Fragments arranged by size: 3172 1071 1016 917 217 85 BsmI GAATG_Cn' Cuts at: 3989 4082 3989 Size: 93 6385 BsmBI CGTCTCn'nnnn_ Cuts at: 3357 3357 Size: 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2389 2570 3306 3523 3797 4539 4624 Size: 399 599 181 736 217 274 742 85 Cuts at: 4624 5695 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 736 599 399 274 217 181 85 BspEI T'CCGG_A Cuts at: 6141 6141 Size: 6478 BspHI T'CATG_A Cuts at: 4367 6097 4367 Size: 1730 4748 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2585 3833 844 Size: 1741 1248 3489 Fragments arranged by size: 3489 1741 1248 BsrBI CCG'CTC Cuts at: 1394 1823 4365 1394 Size: 429 2542 3507 Fragments arranged by size: 3507 2542 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2431 2737 4973 5147 65 Size: 1183 1183 306 2236 174 1396 Fragments arranged by size: 2236 1396 1183 1183 306 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 5122 5309 1700 Size: 110 228 3084 187 2869 Fragments arranged by size: 3084 2869 228 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6478 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6478 BssSI C'ACGA_G Cuts at: 2626 4538 5550 2626 Size: 1912 1012 3554 Fragments arranged by size: 3554 1912 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6478 BstBI TT'CG_AA Cuts at: 5282 5282 Size: 6478 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6478 BstXI CCAn_nnnn'nTGG Cuts at: 3536 3536 Size: 6478 BstYI r'GATC_y Cuts at: 1972 3007 3587 3808 4175 4560 4577 6029 6115 Size: 1035 580 221 367 385 17 1452 86 Cuts at: 6115 6127 6473 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1035 580 385 367 346 221 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2290 3096 3258 513 Size: 552 1225 806 162 3733 Fragments arranged by size: 3733 1225 806 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2380 487 Size: 683 56 1154 4585 Fragments arranged by size: 4585 1154 683 56 BtsI GCAGTG_nn' Cuts at: 3710 3994 4788 4808 3710 Size: 284 794 20 5380 Fragments arranged by size: 5380 794 284 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6478 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2422 2457 3421 3456 529 Size: 35 1858 35 964 35 3551 Fragments arranged by size: 3551 1858 964 35 35 35 DraI TTT'AAA Cuts at: 2779 3154 3782 2779 Size: 375 628 5475 Fragments arranged by size: 5475 628 375 DraIII CAC_nnn'GTG Cuts at: 3566 3566 Size: 6478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2321 3275 5485 809 Size: 507 1005 954 2210 1802 Fragments arranged by size: 2210 1802 1005 954 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2529 3925 4814 Size: 54 1425 50 275 18 699 1396 889 Cuts at: 4814 6443 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1396 889 699 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3925 1537 Size: 275 2113 4090 Fragments arranged by size: 4090 2113 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3017 3168 4408 1353 Size: 1664 151 1240 3423 Fragments arranged by size: 3423 1664 1240 151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3356 5065 5439 5585 387 Size: 1064 138 1767 1709 374 146 1280 Fragments arranged by size: 1767 1709 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2258 2942 3002 3503 3741 4541 Size: 393 445 181 684 60 501 238 800 Cuts at: 4541 5138 5925 6193 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 800 787 684 597 501 445 393 268 238 181 60 EcoNI CCTnn'n_nnAGG Cuts at: 3350 3350 Size: 6478 EcoO109I rG'GnC_Cy Cuts at: 2566 3027 3749 2566 Size: 461 722 5295 Fragments arranged by size: 5295 722 461 EcoRI G'AATT_C Cuts at: 2011 2687 3787 4165 2011 Size: 676 1100 378 4324 Fragments arranged by size: 4324 1100 676 378 FspI TGC'GCA Cuts at: 4984 4984 Size: 6478 HaeII r_GCGC'y Cuts at: 1678 2039 5625 1678 Size: 361 3586 2531 Fragments arranged by size: 3586 2531 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2693 2725 3531 3563 5119 Size: 32 135 32 1121 32 806 32 1556 Cuts at: 5119 5151 5193 5225 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1556 1121 806 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 3822 4068 669 Size: 988 281 196 1688 246 3079 Fragments arranged by size: 3079 1688 988 281 246 196 HindIII A'AGCT_T Cuts at: 748 748 Size: 6478 HpaI GTT'AAC Cuts at: 4068 4068 Size: 6478 KasI G'GCGC_C Cuts at: 2035 2035 Size: 6478 KpnI G_GTAC'C Cuts at: 3803 3803 Size: 6478 MfeI C'AATT_G Cuts at: 4077 4077 Size: 6478 MluI A'CGCG_T Cuts at: 6354 6354 Size: 6478 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3565 5592 5776 1305 Size: 2260 2027 184 2007 Fragments arranged by size: 2260 2027 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2531 10 Size: 54 1425 343 699 3957 Fragments arranged by size: 3957 1425 699 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3135 3260 4436 4795 4954 518 Size: 648 1969 125 1176 359 159 2042 Fragments arranged by size: 2042 1969 1176 648 359 159 125 MspA1I CmG'CkG Cuts at: 1606 3894 4571 5719 5964 1606 Size: 2288 677 1148 245 2120 Fragments arranged by size: 2288 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2036 2036 Size: 6478 NcoI C'CATG_G Cuts at: 513 1065 2290 3096 3258 513 Size: 552 1225 806 162 3733 Fragments arranged by size: 3733 1225 806 552 162 NdeI CA'TA_TG Cuts at: 387 387 Size: 6478 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6478 NotI GC'GGCC_GC Cuts at: 3925 3925 Size: 6478 NspI r_CATG'y Cuts at: 1132 1229 2232 2888 3836 5381 1132 Size: 97 1003 656 948 1545 2229 Fragments arranged by size: 2229 1545 1003 948 656 97 PasI CC'CwG_GG Cuts at: 1348 2068 1348 Size: 720 5758 PciI A'CATG_T Cuts at: 2228 5377 2228 Size: 3149 3329 PflMI CCAn_nnn'nTGG Cuts at: 1108 2022 1108 Size: 914 5564 PfoI T'CCnGG_A Cuts at: 2015 2015 Size: 6478 PmeI GTTT'AAAC Cuts at: 3782 3782 Size: 6478 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4547 4579 6060 6092 4547 Size: 32 1481 32 4933 Fragments arranged by size: 4933 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3027 3027 Size: 6478 PshAI GACnn'nnGTC Cuts at: 1613 2238 1613 Size: 625 5853 PsiI TTA'TAA Cuts at: 2481 4048 2481 Size: 1567 4911 PspOMI G'GGCC_C Cuts at: 2566 2566 Size: 6478 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3727 3759 3727 Size: 32 6446 PstI C_TGCA'G Cuts at: 830 3830 830 Size: 3000 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6478 SalI G'TCGA_C Cuts at: 1936 3820 1936 Size: 1884 4594 SanDI GG'GwC_CC Cuts at: 3027 3027 Size: 6478 SbfI CC_TGCA'GG Cuts at: 3830 3830 Size: 6478 ScaI AGT'ACT Cuts at: 4726 6438 4726 Size: 1712 4766 SfcI C'TryA_G Cuts at: 826 1045 2467 3201 3826 4961 5642 5833 826 Size: 219 1422 734 625 1135 681 191 1471 Fragments arranged by size: 1471 1422 1135 734 681 625 219 191 SfoI GGC'GCC Cuts at: 2037 2037 Size: 6478 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 SgrAI Cr'CCGG_yG Cuts at: 1700 2038 1700 Size: 338 6140 SmaI CCC'GGG Cuts at: 1872 3805 1872 Size: 1933 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4582 5483 5745 820 Size: 197 926 2639 901 262 1553 Fragments arranged by size: 2639 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4269 493 Size: 3776 2702 SpeI A'CTAG_T Cuts at: 152 3421 152 Size: 3269 3209 SphI G_CATG'C Cuts at: 1132 2888 3836 1132 Size: 1756 948 3774 Fragments arranged by size: 3774 1756 948 SspI AAT'ATT Cuts at: 5 52 2793 4402 5 Size: 47 2741 1609 2081 Fragments arranged by size: 2741 2081 1609 47 StuI AGG'CCT Cuts at: 1517 3597 1517 Size: 2080 4398 StyI C'CwwG_G Cuts at: 513 1065 2290 2674 3096 3258 3529 3592 3917 Size: 552 1225 384 422 162 271 63 325 Cuts at: 3917 513 Size: 3074 Fragments arranged by size: 3074 1225 552 422 384 325 271 162 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3182 4505 4522 4675 4860 5305 325 Size: 1255 1602 1323 17 153 185 445 1498 Fragments arranged by size: 1602 1498 1323 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2985 3375 4724 Size: 274 80 33 51 1443 1008 390 1349 Cuts at: 4724 6436 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1349 1008 390 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2215 2247 3354 3386 6438 6470 2215 Size: 32 1107 32 3052 32 2223 Fragments arranged by size: 3052 2223 1107 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6478 XbaI T'CTAG_A Cuts at: 3814 3814 Size: 6478 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6478 XmaI C'CCGG_G Cuts at: 1870 3803 1870 Size: 1933 4545 XmnI GAAnn'nnTTC Cuts at: 4607 4607 Size: 6478 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4286 276 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI