(Linear) (Six-base) MAPSORT of: KIEE2013.seq Check: 3588 from: 1 to: 1773 KIEE2013.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 580 1773 Size: 580 1193 AccI GT'mk_AC Cuts at: 0 1175 1773 Size: 1175 598 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 937 1736 1773 Size: 937 799 37 Fragments arranged by size: 937 799 37 AflIII A'CryG_T Cuts at: 0 223 1773 Size: 223 1550 AleI CACnn'nnGTG Cuts at: 0 1255 1773 Size: 1255 518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 172 612 1773 Size: 172 440 1161 Fragments arranged by size: 1161 440 172 AlwNI CAG_nnn'CTG Cuts at: 0 1042 1326 1476 1747 1773 Size: 1042 284 150 271 26 Fragments arranged by size: 1042 284 271 150 26 ApaI G_GGCC'C Cuts at: 0 565 1773 Size: 565 1208 ApaLI G'TGCA_C Cuts at: 0 380 1773 Size: 380 1393 ApoI r'AATT_y Cuts at: 0 6 537 682 1773 Size: 6 531 145 1091 Fragments arranged by size: 1091 531 145 6 BamHI G'GATC_C Cuts at: 0 1582 1773 Size: 1582 191 BanI G'GyrC_C Cuts at: 0 30 1159 1773 Size: 30 1129 614 Fragments arranged by size: 1129 614 30 BanII G_rGCy'C Cuts at: 0 565 1773 Size: 565 1208 BbeI G_GCGC'C Cuts at: 0 34 1773 Size: 34 1739 BbsI GAAGACnn'nnnn_ Cuts at: 0 329 1655 1683 1773 Size: 329 1326 28 90 Fragments arranged by size: 1326 329 90 28 BclI T'GATC_A Cuts at: 0 573 1773 Size: 573 1200 BglII A'GATC_T Cuts at: 0 1002 1773 Size: 1002 771 Bme1580I G_kGCm'C Cuts at: 0 384 565 1773 Size: 384 181 1208 Fragments arranged by size: 1208 384 181 BmrI ACTGGGnnnn_n' Cuts at: 0 516 559 1773 Size: 516 43 1214 Fragments arranged by size: 1214 516 43 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 253 997 1498 1773 Size: 72 181 744 501 275 Fragments arranged by size: 744 501 275 181 72 BsaI GGTCTCn'nnnn_ Cuts at: 0 434 1611 1773 Size: 434 1177 162 Fragments arranged by size: 1177 434 162 BsaHI Gr'CG_yC Cuts at: 0 31 1773 Size: 31 1742 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1349 1379 1393 1423 1526 1556 1773 Size: 1349 30 14 30 103 30 217 Fragments arranged by size: 1349 217 103 30 30 30 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1115 1298 1495 1706 1773 Size: 1115 183 197 211 67 Fragments arranged by size: 1115 211 197 183 67 BseYI C'CCAG_C Cuts at: 0 1377 1773 Size: 1377 396 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1387 1773 Size: 1387 386 BsiHKAI G_wGCw'C Cuts at: 0 384 1301 1518 1773 Size: 384 917 217 255 Fragments arranged by size: 917 384 255 217 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1352 1773 Size: 1352 421 Bsp1286I G_dGCh'C Cuts at: 0 384 565 1301 1518 1773 Size: 384 181 736 217 255 Fragments arranged by size: 736 384 255 217 181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 580 1773 Size: 580 1193 BsrDI GCAATG_nn' Cuts at: 0 426 732 1773 Size: 426 306 1041 Fragments arranged by size: 1041 426 306 BsrFI r'CCGG_y Cuts at: 0 33 1773 Size: 33 1740 BssSI C'ACGA_G Cuts at: 0 621 1773 Size: 621 1152 BstXI CCAn_nnnn'nTGG Cuts at: 0 1531 1773 Size: 1531 242 BstYI r'GATC_y Cuts at: 0 1002 1582 1773 Size: 1002 580 191 Fragments arranged by size: 1002 580 191 BtgI C'CryG_G Cuts at: 0 285 1091 1253 1773 Size: 285 806 162 520 Fragments arranged by size: 806 520 285 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 375 1773 Size: 375 1398 BtsI GCAGTG_nn' Cuts at: 0 1705 1773 Size: 1705 68 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 417 452 1416 1451 1773 Size: 417 35 964 35 322 Fragments arranged by size: 964 417 322 35 35 DraI TTT'AAA Cuts at: 0 774 1149 1773 Size: 774 375 624 Fragments arranged by size: 774 624 375 DraIII CAC_nnn'GTG Cuts at: 0 1561 1773 Size: 1561 212 DrdI GACnn_nn'nnGTC Cuts at: 0 316 1270 1773 Size: 316 954 503 Fragments arranged by size: 954 503 316 EaeI y'GGCC_r Cuts at: 0 524 1773 Size: 524 1249 EarI CTCTTCn'nnn_ Cuts at: 0 1012 1163 1773 Size: 1012 151 610 Fragments arranged by size: 1012 610 151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1351 1773 Size: 1351 422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 253 937 997 1498 1736 1773 Size: 72 181 684 60 501 238 37 Fragments arranged by size: 684 501 238 181 72 60 37 EcoNI CCTnn'n_nnAGG Cuts at: 0 1345 1773 Size: 1345 428 EcoO109I rG'GnC_Cy Cuts at: 0 561 1022 1744 1773 Size: 561 461 722 29 Fragments arranged by size: 722 561 461 29 EcoRI G'AATT_C Cuts at: 0 6 682 1773 Size: 6 676 1091 Fragments arranged by size: 1091 676 6 HaeII r_GCGC'y Cuts at: 0 34 1773 Size: 34 1739 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 688 720 1526 1558 1773 Size: 688 32 806 32 215 Fragments arranged by size: 806 688 215 32 32 HincII GTy'rAC Cuts at: 0 129 1773 Size: 129 1644 KasI G'GCGC_C Cuts at: 0 30 1773 Size: 30 1743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1560 1773 Size: 1560 213 MscI TGG'CCA Cuts at: 0 526 1773 Size: 526 1247 MslI CAynn'nnrTG Cuts at: 0 1130 1255 1773 Size: 1130 125 518 Fragments arranged by size: 1130 518 125 NarI GG'CG_CC Cuts at: 0 31 1773 Size: 31 1742 NcoI C'CATG_G Cuts at: 0 285 1091 1253 1773 Size: 285 806 162 520 Fragments arranged by size: 806 520 285 162 NspI r_CATG'y Cuts at: 0 227 883 1773 Size: 227 656 890 Fragments arranged by size: 890 656 227 PasI CC'CwG_GG Cuts at: 0 63 1773 Size: 63 1710 PciI A'CATG_T Cuts at: 0 223 1773 Size: 223 1550 PflMI CCAn_nnn'nTGG Cuts at: 0 17 1773 Size: 17 1756 PfoI T'CCnGG_A Cuts at: 0 10 1773 Size: 10 1763 PpuMI rG'GwC_Cy Cuts at: 0 1022 1773 Size: 1022 751 PshAI GACnn'nnGTC Cuts at: 0 233 1773 Size: 233 1540 PsiI TTA'TAA Cuts at: 0 476 1773 Size: 476 1297 PspOMI G'GGCC_C Cuts at: 0 561 1773 Size: 561 1212 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1722 1754 1773 Size: 1722 32 19 Fragments arranged by size: 1722 32 19 SanDI GG'GwC_CC Cuts at: 0 1022 1773 Size: 1022 751 SfcI C'TryA_G Cuts at: 0 462 1196 1773 Size: 462 734 577 Fragments arranged by size: 734 577 462 SfoI GGC'GCC Cuts at: 0 32 1773 Size: 32 1741 SgrAI Cr'CCGG_yG Cuts at: 0 33 1773 Size: 33 1740 SpeI A'CTAG_T Cuts at: 0 1416 1773 Size: 1416 357 SphI G_CATG'C Cuts at: 0 883 1773 Size: 883 890 SspI AAT'ATT Cuts at: 0 788 1773 Size: 788 985 StuI AGG'CCT Cuts at: 0 1592 1773 Size: 1592 181 StyI C'CwwG_G Cuts at: 0 285 669 1091 1253 1524 1587 1773 Size: 285 384 422 162 271 63 186 Fragments arranged by size: 422 384 285 271 186 162 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1177 1773 Size: 1177 596 TatI w'GTAC_w Cuts at: 0 980 1370 1773 Size: 980 390 403 Fragments arranged by size: 980 403 390 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 210 242 1349 1381 1773 Size: 210 32 1107 32 392 Fragments arranged by size: 1107 392 210 32 32 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbeI BbsI BclI BglII Bme1580I BmrI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI MscI MslI NarI NcoI NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PsrI SanDI SfcI SfoI SgrAI SpeI SphI SspI StuI StyI TaqII TatI TstI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI EagI EcoICRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SmaI SmlI SnaBI SrfI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI