ADGRE3 ORF clone FHC26501

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Halotag ORF Clone FHC26501

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Clone info.

Species

human

Clone Name

pFN21AE9452 Sequence (with vector) Restriction Map

Product ID

FHC26501

ORF Sequence

DNA sequence (1956 bp)
Protein sequence (652 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9452

Symbol

ADGRE3

Alias : EMR3

Description

adhesion G protein-coupled receptor E3, transcript variant 1

Original Clone ID

cp99452

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F3)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

Features of the cloned ORF sequence

Description

Length: 1956 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 652 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12315.1 1.1e-208 99.7 ADGRE3 CCDS74297.1 9.8e-170 91.7 ADGRE3 CCDS74296.1 5.7e-144 80.5 ADGRE3 CCDS32935.1 2.8e-81 51.5 ADGRE2 CCDS59361.1 3.9e-71 49.7 ADGRE2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001740 266 283 PR01128 GPCR IPR001740 288 307 PR01128 GPCR IPR001740 307 325 PR01128 GPCR IPR000832 358 382 PR00249 GPCR IPR001740 381 398 PR01128 GPCR IPR000832 421 444 PR00249 GPCR IPR001740 449 468 PR01128 GPCR IPR001740 482 496 PR01128 GPCR IPR000832 506 531 PR00249 GPCR IPR001740 540 561 PR01128 GPCR IPR000832 580 601 PR00249 GPCR IPR001740 618 642 PR01128 GPCR HMMPfam IPR013091 67 116 PF07645 EGF calcium-binding IPR000203 303 345 PF01825 GPS domain IPR000832 355 594 PF00002 GPCR HMMSmart IPR006210 27 66 SM00181 Epidermal growth factor-like IPR001881 67 118 SM00179 EGF-like calcium-binding IPR006210 70 118 SM00181 Epidermal growth factor-like IPR000203 300 350 SM00303 GPS domain ProfileScan IPR000742 67 106 PS50026 Epidermal growth factor-like IPR000203 301 350 PS50221 GPS domain IPR017981 356 602 PS50261 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 4 PLLLPGLCFLLSLFGAVTQK 23 SECONDARY 20 2 358 VITYVGLSVSLLCLLLAALTFLL 380 PRIMARY 23 3 390 SLHLQLSLCLFLAHLLFLVGIDR 412 PRIMARY 23 4 421 IIAGALHYLYLAAFTWMLLEGVH 443 PRIMARY 23 5 465 WIMFPVGYGVPAVTVAISAASWP 487 SECONDARY 23 6 509 LGPVCAIFSANLVLFILVFWIL 530 PRIMARY 22 7 554 ATAQLFILGCTWCLGLLQVGPAA 576 PRIMARY 23 8 582 LFTIINSLQGFFIFLVYCLLSQQ 604 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_115960 652 606101 99.7 100.0 XP_011526676 635 606101 96.9 100.0 NP_001276087 600 606101 91.7 100.0 NP_001276088 526 606101 80.5 100.0 XP_011526251 826 125630,606100 51.8 93.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9452 Download

>KIEE9452  1956 bp
ATGCAGGGACCATTGCTTCTTCCAGGCCTCTGCTTTCTGCTGAGCCTCTTTGGAGCTGTG
ACTCAGAAAACCAAAACTTCCTGTGCTAAGTGCCCCCCAAATGCTTCCTGTGTCAATAAC
ACTCACTGCACCTGCAACCATGGATATACTTCTGGATCTGGGCAGAAACTATTCACATTC
CCCTTGGAGACATGTAACGACATTAATGAATGTACACCACCCTATAGTGTATATTGTGGA
TTTAACGCTGTGTGTTACAATGTCGAAGGAAGTTTCTACTGTCAATGTGTCCCAGGATAT
AGACTGCATTCTGGGAATGAACAATTCAGTAATTCCAATGAGAACACCTGTCAGGACACC
ACCTCCTCAAAGACAACCCAGGGCAGGAAAGAGCTGCAAAAGATTGTGGACAAATTTGAG
TCACTTCTCACCAATCAGACTTTATGGAGAACAGAAGGGAGACAAGAAATCTCATCCACA
GCTACCACTATTCTCCGGGATGTGGAATCGAAAGTTCTAGAAACTGCCTTGAAAGATCCA
GAACAAAAAGTCCTGAAAATCCAAAACGATAGTGTAGCTATTGAAACTCAAGCGATTACA
GACAATTGCTCTGAAGAAAGAAAGACATTCAACTTGAACGTCCAAATGAACTCAATGGAC
ATCCGTTGCAGTGACATCATCCAGGGAGACACACAAGGTCCCAGTGCCATTGCCTTTATC
TCATATTCTTCTCTTGGAAACATCATAAATGCAACTTTTTTTGAAGAGATGGATAAGAAA
GATCAAGTGTATCTGAACTCTCAGGTTGTGAGTGCTGCTATTGGACCCAAAAGGAACGTG
TCTCTCTCCAAGTCTGTGACGCTGACTTTCCAGCACGTGAAGATGACCCCCAGTACCAAA
AAGGTCTTCTGTGTCTACTGGAAGAGCACAGGGCAGGGCAGCCAGTGGTCCAGGGATGGC
TGCTTCCTGATACACGTGAACAAGAGTCACACCATGTGTAATTGCAGTCACCTGTCCAGC
TTCGCTGTCCTGATGGCCCTGACCAGCCAGGAGGAGGATCCCGTGCTGACTGTCATCACC
TACGTGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTG
TGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTC
CTGGCCCACCTCCTCTTCCTCGTGGGGATTGATCGAACTGAACCCAAGGTGCTGTGCTCC
ATCATCGCCGGTGCTTTGCACTATCTCTACCTGGCCGCCTTCACCTGGATGCTGCTGGAG
GGTGTGCACCTCTTCCTCACTGCACGGAACCTGACAGTGGTCAACTACTCAAGCATCAAT
AGACTCATGAAGTGGATCATGTTCCCAGTCGGCTATGGCGTTCCCGCTGTGACTGTGGCC
ATTTCTGCAGCCTCCTGGCCTCACCTTTATGGAACTGCTGATCGATGCTGGCTCCACCTG
GACCAGGGATTCATGTGGAGTTTCCTTGGCCCAGTCTGTGCCATTTTCTCTGCGAATTTA
GTATTGTTTATCTTGGTCTTTTGGATTTTGAAAAGAAAACTTTCCTCCCTCAATAGTGAA
GTGTCAACCATCCAGAACACAAGGATGCTGGCTTTCAAAGCAACAGCTCAGCTCTTCATC
CTGGGCTGCACATGGTGTCTGGGCTTGCTACAGGTGGGTCCAGCTGCCCAGGTCATGGCC
TACCTCTTCACCATCATCAACAGCCTCCAAGGCTTCTTCATCTTCTTGGTCTACTGCCTC
CTCAGCCAGCAGGTCCAGAAACAATATCAAAAGTGGTTTAGAGAGATCGTAAAATCAAAA
TCTGAGTCTGAGACATACACACTTTCCAGCAAGATGGGTCCTGACTCAAAACCCAGTGAG
GGGGATGTTTTTCCAGGACAAGTGAAGAGAAAATAT

ORF Protein

Cloned ORF protein sequence for pFN21AE9452 Download

>KIEE9452  652 aa
MQGPLLLPGLCFLLSLFGAVTQKTKTSCAKCPPNASCVNNTHCTCNHGYTSGSGQKLFTF
PLETCNDINECTPPYSVYCGFNAVCYNVEGSFYCQCVPGYRLHSGNEQFSNSNENTCQDT
TSSKTTQGRKELQKIVDKFESLLTNQTLWRTEGRQEISSTATTILRDVESKVLETALKDP
EQKVLKIQNDSVAIETQAITDNCSEERKTFNLNVQMNSMDIRCSDIIQGDTQGPSAIAFI
SYSSLGNIINATFFEEMDKKDQVYLNSQVVSAAIGPKRNVSLSKSVTLTFQHVKMTPSTK
KVFCVYWKSTGQGSQWSRDGCFLIHVNKSHTMCNCSHLSSFAVLMALTSQEEDPVLTVIT
YVGLSVSLLCLLLAALTFLLCKAIQNTSTSLHLQLSLCLFLAHLLFLVGIDRTEPKVLCS
IIAGALHYLYLAAFTWMLLEGVHLFLTARNLTVVNYSSINRLMKWIMFPVGYGVPAVTVA
ISAASWPHLYGTADRCWLHLDQGFMWSFLGPVCAIFSANLVLFILVFWILKRKLSSLNSE
VSTIQNTRMLAFKATAQLFILGCTWCLGLLQVGPAAQVMAYLFTIINSLQGFFIFLVYCL
LSQQVQKQYQKWFREIVKSKSESETYTLSSKMGPDSKPSEGDVFPGQVKRKY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9452 Download

>pFN21AE9452  6661 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCAGGGACCATTGCTTCTTCCAGGCCTCTGCTT
TCTGCTGAGCCTCTTTGGAGCTGTGACTCAGAAAACCAAAACTTCCTGTGCTAAGTGCCC
CCCAAATGCTTCCTGTGTCAATAACACTCACTGCACCTGCAACCATGGATATACTTCTGG
ATCTGGGCAGAAACTATTCACATTCCCCTTGGAGACATGTAACGACATTAATGAATGTAC
ACCACCCTATAGTGTATATTGTGGATTTAACGCTGTGTGTTACAATGTCGAAGGAAGTTT
CTACTGTCAATGTGTCCCAGGATATAGACTGCATTCTGGGAATGAACAATTCAGTAATTC
CAATGAGAACACCTGTCAGGACACCACCTCCTCAAAGACAACCCAGGGCAGGAAAGAGCT
GCAAAAGATTGTGGACAAATTTGAGTCACTTCTCACCAATCAGACTTTATGGAGAACAGA
AGGGAGACAAGAAATCTCATCCACAGCTACCACTATTCTCCGGGATGTGGAATCGAAAGT
TCTAGAAACTGCCTTGAAAGATCCAGAACAAAAAGTCCTGAAAATCCAAAACGATAGTGT
AGCTATTGAAACTCAAGCGATTACAGACAATTGCTCTGAAGAAAGAAAGACATTCAACTT
GAACGTCCAAATGAACTCAATGGACATCCGTTGCAGTGACATCATCCAGGGAGACACACA
AGGTCCCAGTGCCATTGCCTTTATCTCATATTCTTCTCTTGGAAACATCATAAATGCAAC
TTTTTTTGAAGAGATGGATAAGAAAGATCAAGTGTATCTGAACTCTCAGGTTGTGAGTGC
TGCTATTGGACCCAAAAGGAACGTGTCTCTCTCCAAGTCTGTGACGCTGACTTTCCAGCA
CGTGAAGATGACCCCCAGTACCAAAAAGGTCTTCTGTGTCTACTGGAAGAGCACAGGGCA
GGGCAGCCAGTGGTCCAGGGATGGCTGCTTCCTGATACACGTGAACAAGAGTCACACCAT
GTGTAATTGCAGTCACCTGTCCAGCTTCGCTGTCCTGATGGCCCTGACCAGCCAGGAGGA
GGATCCCGTGCTGACTGTCATCACCTACGTGGGGCTGAGCGTCTCTCTGCTGTGCCTCCT
CCTGGCGGCCCTCACTTTTCTCCTGTGTAAAGCCATCCAGAACACCAGCACCTCACTGCA
TCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACCTCCTCTTCCTCGTGGGGATTGATCG
AACTGAACCCAAGGTGCTGTGCTCCATCATCGCCGGTGCTTTGCACTATCTCTACCTGGC
CGCCTTCACCTGGATGCTGCTGGAGGGTGTGCACCTCTTCCTCACTGCACGGAACCTGAC
AGTGGTCAACTACTCAAGCATCAATAGACTCATGAAGTGGATCATGTTCCCAGTCGGCTA
TGGCGTTCCCGCTGTGACTGTGGCCATTTCTGCAGCCTCCTGGCCTCACCTTTATGGAAC
TGCTGATCGATGCTGGCTCCACCTGGACCAGGGATTCATGTGGAGTTTCCTTGGCCCAGT
CTGTGCCATTTTCTCTGCGAATTTAGTATTGTTTATCTTGGTCTTTTGGATTTTGAAAAG
AAAACTTTCCTCCCTCAATAGTGAAGTGTCAACCATCCAGAACACAAGGATGCTGGCTTT
CAAAGCAACAGCTCAGCTCTTCATCCTGGGCTGCACATGGTGTCTGGGCTTGCTACAGGT
GGGTCCAGCTGCCCAGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTCCAAGGCTT
CTTCATCTTCTTGGTCTACTGCCTCCTCAGCCAGCAGGTCCAGAAACAATATCAAAAGTG
GTTTAGAGAGATCGTAAAATCAAAATCTGAGTCTGAGACATACACACTTTCCAGCAAGAT
GGGTCCTGACTCAAAACCCAGTGAGGGGGATGTTTTTCCAGGACAAGTGAAGAGAAAATA
TGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T