(Circular) (Six-base) MAPSORT of: pFN21AE9452.seq Check: 4226 from: 1 to: 6661 pFN21AE9452 6661 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2144 2144 Size: 6661 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4471 278 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2919 3795 4004 1937 Size: 982 876 209 4594 Fragments arranged by size: 4594 982 876 209 Acc65I G'GTAC_C Cuts at: 3982 3982 Size: 6661 AclI AA'CG_TT Cuts at: 1563 4788 5161 1563 Size: 3225 373 3063 Fragments arranged by size: 3225 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2637 4724 6108 6376 1632 Size: 1005 2087 1384 268 1917 Fragments arranged by size: 2087 1917 1384 1005 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6464 AflIII A'CryG_T Cuts at: 1203 2195 2841 2977 5560 6537 1203 Size: 992 646 136 2583 977 1327 Fragments arranged by size: 2583 1327 992 977 646 136 AgeI A'CCGG_T Cuts at: 5492 5492 Size: 6661 AhdI GACnn_n'nnGTC Cuts at: 5390 5390 Size: 6661 AleI CACnn'nnGTG Cuts at: 1166 3086 1166 Size: 1920 4741 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3191 1184 Size: 2007 4654 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2538 2570 2538 Size: 32 6629 AlwNI CAG_nnn'CTG Cuts at: 4404 5976 4404 Size: 1572 5089 ApaLI G'TGCA_C Cuts at: 3329 4718 5874 3329 Size: 1389 1156 4116 Fragments arranged by size: 4116 1389 1156 ApoI r'AATT_y Cuts at: 1493 2417 3559 3970 4196 4348 6457 1493 Size: 924 1142 411 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 924 411 226 152 AseI AT'TA_AT Cuts at: 160 1032 2208 5215 160 Size: 872 1176 3007 1606 Fragments arranged by size: 3007 1606 1176 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 AvaI C'yCGr_G Cuts at: 1870 1943 3986 1870 Size: 73 2043 4545 Fragments arranged by size: 4545 2043 73 BamHI G'GATC_C Cuts at: 3061 3991 4358 3061 Size: 930 367 5364 Fragments arranged by size: 5364 930 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3982 5437 618 Size: 325 217 627 2195 1455 1842 Fragments arranged by size: 2195 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3980 1391 Size: 2589 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2902 928 Size: 971 1003 4687 Fragments arranged by size: 4687 1003 971 BbvCI CC'TCA_GC Cuts at: 3806 3806 Size: 6661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4852 4886 1903 Size: 34 50 34 2831 34 3678 Fragments arranged by size: 3678 2831 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4553 5763 4553 Size: 1210 5451 BclI T'GATC_A Cuts at: 1548 1548 Size: 6661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3774 5272 136 Size: 107 122 71 631 605 2102 1498 1525 Fragments arranged by size: 2102 1525 1498 631 605 122 107 71 BglII A'GATC_T Cuts at: 6656 6656 Size: 6661 BlpI GC'TnA_GC Cuts at: 2045 3095 3672 4078 2045 Size: 1050 577 406 4628 Fragments arranged by size: 4628 1050 577 406 Bme1580I G_kGCm'C Cuts at: 1790 2099 3333 4722 5878 1790 Size: 309 1234 1389 1156 2573 Fragments arranged by size: 2573 1389 1234 1156 309 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6661 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2700 2889 3404 Size: 416 53 83 399 24 1283 189 515 Cuts at: 3404 3530 3912 4392 5350 442 Size: 126 382 480 958 1753 Fragments arranged by size: 1753 1283 958 515 480 416 399 382 189 126 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6661 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3120 3120 Size: 6661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3341 5321 1239 Size: 2102 1980 2579 Fragments arranged by size: 2579 2102 1980 Bpu10I CC'TnA_GC Cuts at: 3806 3806 Size: 6661 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2577 3358 4786 5651 5949 2577 Size: 781 1428 865 298 3289 Fragments arranged by size: 3289 1428 865 781 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5324 882 Size: 617 3825 2219 Fragments arranged by size: 3825 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2881 2980 3088 4452 493 Size: 711 1677 99 108 1364 2702 Fragments arranged by size: 2702 1677 1364 711 108 99 BsaBI GATnn'nnATC Cuts at: 6655 6655 Size: 6661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4468 4850 5485 275 Size: 53 83 186 936 2935 382 635 1451 Fragments arranged by size: 2935 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5094 5492 5766 5913 6324 5094 Size: 398 274 147 411 5431 Fragments arranged by size: 5431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2351 2381 1178 Size: 30 165 30 948 30 5458 Fragments arranged by size: 5458 948 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2359 3071 3107 3206 3794 1186 Size: 1173 712 36 99 588 4053 Fragments arranged by size: 4053 1173 712 588 99 36 BseYI C'CCAG_C Cuts at: 5864 6360 5864 Size: 496 6165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2116 3330 3676 1163 Size: 573 380 1214 346 4148 Fragments arranged by size: 4148 1214 573 380 346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4111 4872 5900 1540 Size: 275 186 2110 761 1028 2301 Fragments arranged by size: 2301 2110 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2933 3263 3333 4722 4807 5878 2933 Size: 330 70 1389 85 1071 3716 Fragments arranged by size: 3716 1389 1071 330 85 70 BsmI GAATG_Cn' Cuts at: 2311 4172 4265 2311 Size: 1861 93 4707 Fragments arranged by size: 4707 1861 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3106 3106 Size: 6661 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2933 3263 3333 3980 4722 4807 Size: 399 309 834 330 70 647 742 85 Cuts at: 4807 5878 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 834 742 647 399 330 309 85 70 BspEI T'CCGG_A Cuts at: 6324 6324 Size: 6661 BspHI T'CATG_A Cuts at: 3390 4550 6280 3390 Size: 1160 1730 3771 Fragments arranged by size: 3771 1730 1160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2144 3805 4016 844 Size: 1300 1661 211 3489 Fragments arranged by size: 3489 1661 1300 211 BsrBI CCG'CTC Cuts at: 1394 1823 4548 1394 Size: 429 2725 3507 Fragments arranged by size: 3507 2725 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2015 2712 5156 5330 65 Size: 1183 767 697 2444 174 1396 Fragments arranged by size: 2444 1396 1183 767 697 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3272 5305 5492 1700 Size: 110 1462 2033 187 2869 Fragments arranged by size: 2869 2033 1462 187 110 BsrGI T'GTAC_A Cuts at: 96 2216 96 Size: 2120 4541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6661 BssSI C'ACGA_G Cuts at: 3224 4721 5733 3224 Size: 1497 1012 4152 Fragments arranged by size: 4152 1497 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6661 BstBI TT'CG_AA Cuts at: 5465 5465 Size: 6661 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6661 BstXI CCAn_nnnn'nTGG Cuts at: 3898 3898 Size: 6661 BstYI r'GATC_y Cuts at: 1972 2159 2539 3061 3991 4358 4743 4760 6212 Size: 187 380 522 930 367 385 17 1452 Cuts at: 6212 6298 6310 6656 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 930 522 385 380 367 346 187 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2143 513 Size: 552 1078 5031 Fragments arranged by size: 5031 1078 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3253 487 Size: 683 56 2027 3895 Fragments arranged by size: 3895 2027 683 56 BtsI GCAGTG_nn' Cuts at: 2128 2680 3173 3342 4177 4971 4991 2128 Size: 552 493 169 835 794 20 3798 Fragments arranged by size: 3798 835 794 552 493 169 20 ClaI AT'CG_AT Cuts at: 1554 3487 1554 Size: 1933 4728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6626 DraI TTT'AAA Cuts at: 3965 3965 Size: 6661 DraIII CAC_nnn'GTG Cuts at: 3000 3699 3000 Size: 699 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5668 809 Size: 507 4352 1802 Fragments arranged by size: 4352 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3297 3441 4108 Size: 54 1425 50 275 18 1467 144 667 Cuts at: 4108 4997 6626 8 Size: 889 1629 43 Fragments arranged by size: 1629 1467 1425 889 667 275 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 1537 Size: 275 2296 4090 Fragments arranged by size: 4090 2296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2763 2921 3206 3224 3341 3683 3755 3944 Size: 1410 158 285 18 117 342 72 189 Cuts at: 3944 4591 1353 Size: 647 3423 Fragments arranged by size: 3423 1410 647 342 285 189 158 117 72 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5248 5622 5768 387 Size: 1064 138 3659 374 146 1280 Fragments arranged by size: 3659 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2637 3341 4724 5321 6108 6376 1239 Size: 393 1005 704 1383 597 787 268 1524 Fragments arranged by size: 1524 1383 1005 787 704 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3506 3506 Size: 6661 EcoO109I rG'GnC_Cy Cuts at: 2702 3902 2702 Size: 1200 5461 EcoRI G'AATT_C Cuts at: 3970 4348 3970 Size: 378 6283 FspI TGC'GCA Cuts at: 5167 5167 Size: 6661 HaeII r_GCGC'y Cuts at: 1678 5808 1678 Size: 4130 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 2457 2489 2495 Size: 32 135 32 779 32 74 32 6 Cuts at: 2495 2527 2597 2629 3480 3512 5302 5334 5376 Size: 32 70 32 851 32 1790 32 42 Cuts at: 5376 5408 1373 Size: 32 2626 Fragments arranged by size: 2626 1790 851 779 135 74 70 42 32 32 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 3367 3630 4005 4251 669 Size: 988 281 1429 263 375 246 3079 Fragments arranged by size: 3079 1429 988 375 281 263 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6661 HpaI GTT'AAC Cuts at: 4251 4251 Size: 6661 KpnI G_GTAC'C Cuts at: 3986 3986 Size: 6661 MfeI C'AATT_G Cuts at: 2608 4260 2608 Size: 1652 5009 MluI A'CGCG_T Cuts at: 6537 6537 Size: 6661 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5775 5959 1305 Size: 4470 184 2007 Fragments arranged by size: 4470 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3443 10 Size: 54 1425 343 1611 3228 Fragments arranged by size: 3228 1611 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3086 3311 3471 3647 4619 4978 5137 Size: 648 1920 225 160 176 972 359 159 Cuts at: 5137 518 Size: 2042 Fragments arranged by size: 2042 1920 972 648 359 225 176 160 159 MspA1I CmG'CkG Cuts at: 1606 3431 3728 4077 4754 5902 6147 1606 Size: 1825 297 349 677 1148 245 2120 Fragments arranged by size: 2120 1825 1148 677 349 297 245 NcoI C'CATG_G Cuts at: 513 1065 2143 513 Size: 552 1078 5031 Fragments arranged by size: 5031 1078 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6661 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6661 NotI GC'GGCC_GC Cuts at: 4108 4108 Size: 6661 NspI r_CATG'y Cuts at: 1132 1229 2199 4019 5564 1132 Size: 97 970 1820 1545 2229 Fragments arranged by size: 2229 1820 1545 970 97 PasI CC'CwG_GG Cuts at: 1348 2383 1348 Size: 1035 5626 PciI A'CATG_T Cuts at: 2195 5560 2195 Size: 3365 3296 PflMI CCAn_nnn'nTGG Cuts at: 1108 3739 1108 Size: 2631 4030 PfoI T'CCnGG_A Cuts at: 2499 3937 2499 Size: 1438 5223 PmeI GTTT'AAAC Cuts at: 3965 3965 Size: 6661 PmlI CAC'GTG Cuts at: 2881 2980 2881 Size: 99 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4730 4762 6243 6275 4730 Size: 32 1481 32 5116 Fragments arranged by size: 5116 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2702 3902 2702 Size: 1200 5461 PshAI GACnn'nnGTC Cuts at: 1613 3362 1613 Size: 1749 4912 PsiI TTA'TAA Cuts at: 4231 4231 Size: 6661 PstI C_TGCA'G Cuts at: 830 3186 3454 4013 830 Size: 2356 268 559 3478 Fragments arranged by size: 3478 2356 559 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6661 PvuII CAG'CTG Cuts at: 3728 3728 Size: 6661 SalI G'TCGA_C Cuts at: 1936 4003 1936 Size: 2067 4594 SapI GCTCTTCn'nnn_ Cuts at: 2921 3683 2921 Size: 762 5899 SbfI CC_TGCA'GG Cuts at: 4013 4013 Size: 6661 ScaI AGT'ACT Cuts at: 4909 6621 4909 Size: 1712 4949 SfcI C'TryA_G Cuts at: 826 1045 2227 3182 3450 3713 4009 5144 5825 Size: 219 1182 955 268 263 296 1135 681 Cuts at: 5825 6016 826 Size: 191 1471 Fragments arranged by size: 1471 1182 1135 955 681 296 268 263 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 SgrAI Cr'CCGG_yG Cuts at: 1700 3272 1700 Size: 1572 5089 SmaI CCC'GGG Cuts at: 1872 3988 1872 Size: 2116 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2592 3373 4765 5666 5928 820 Size: 197 926 649 781 1392 901 262 1553 Fragments arranged by size: 1553 1392 926 901 781 649 262 197 SnaBI TAC'GTA Cuts at: 493 4452 493 Size: 3959 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6661 SphI G_CATG'C Cuts at: 1132 4019 1132 Size: 2887 3774 SspI AAT'ATT Cuts at: 5 52 4585 5 Size: 47 4533 2081 Fragments arranged by size: 4533 2081 47 StuI AGG'CCT Cuts at: 1517 2031 1517 Size: 514 6147 StyI C'CwwG_G Cuts at: 513 1065 2143 2187 3249 3529 3772 4100 513 Size: 552 1078 44 1062 280 243 328 3074 Fragments arranged by size: 3074 1078 1062 552 328 280 243 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4688 4705 4858 5043 5488 325 Size: 1255 3108 17 153 185 445 1498 Fragments arranged by size: 3108 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2216 4907 6619 Size: 274 80 33 51 1443 239 2691 1712 Cuts at: 6619 96 Size: 138 Fragments arranged by size: 2691 1712 1443 274 239 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2686 2718 6621 6653 2686 Size: 32 3903 32 2694 Fragments arranged by size: 3903 2694 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6661 XbaI T'CTAG_A Cuts at: 2521 3997 2521 Size: 1476 5185 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6661 XmaI C'CCGG_G Cuts at: 1870 3986 1870 Size: 2116 4545 XmnI GAAnn'nnTTC Cuts at: 2174 3604 4790 2174 Size: 1430 1186 4045 Fragments arranged by size: 4045 1430 1186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4469 276 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI