(Linear) (Six-base) MAPSORT of: KIEE9452.seq Check: 3814 from: 1 to: 1956 KIEE9452.orf With 182 enzymes: SgfI * January 29, 2014 12:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 139 1956 Size: 139 1817 AccI GT'mk_AC Cuts at: 0 914 1790 1956 Size: 914 876 166 Fragments arranged by size: 914 876 166 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 632 1956 Size: 632 1324 AflIII A'CryG_T Cuts at: 0 190 836 972 1956 Size: 190 646 136 984 Fragments arranged by size: 984 646 190 136 AleI CACnn'nnGTG Cuts at: 0 1081 1956 Size: 1081 875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1186 1956 Size: 1186 770 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 533 565 1956 Size: 533 32 1391 Fragments arranged by size: 1391 533 32 ApaLI G'TGCA_C Cuts at: 0 1324 1956 Size: 1324 632 ApoI r'AATT_y Cuts at: 0 412 1554 1956 Size: 412 1142 402 Fragments arranged by size: 1142 412 402 AseI AT'TA_AT Cuts at: 0 203 1956 Size: 203 1753 BamHI G'GATC_C Cuts at: 0 1056 1956 Size: 1056 900 BbsI GAAGACnn'nnnn_ Cuts at: 0 897 1956 Size: 897 1059 BbvCI CC'TCA_GC Cuts at: 0 1801 1956 Size: 1801 155 BglI GCCn_nnn'nGGC Cuts at: 0 1769 1956 Size: 1769 187 BlpI GC'TnA_GC Cuts at: 0 40 1090 1667 1956 Size: 40 1050 577 289 Fragments arranged by size: 1050 577 289 40 Bme1580I G_kGCm'C Cuts at: 0 94 1328 1956 Size: 94 1234 628 Fragments arranged by size: 1234 628 94 BmrI ACTGGGnnnn_n' Cuts at: 0 695 884 1399 1525 1907 1956 Size: 695 189 515 126 382 49 Fragments arranged by size: 695 515 382 189 126 49 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1115 1956 Size: 1115 841 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1336 1956 Size: 1336 620 Bpu10I CC'TnA_GC Cuts at: 0 1801 1956 Size: 1801 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 572 1353 1956 Size: 572 781 603 Fragments arranged by size: 781 603 572 BsaAI yAC'GTr Cuts at: 0 876 975 1083 1956 Size: 876 99 108 873 Fragments arranged by size: 876 873 108 99 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 346 376 1956 Size: 346 30 1580 Fragments arranged by size: 1580 346 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 354 1066 1102 1201 1789 1956 Size: 354 712 36 99 588 167 Fragments arranged by size: 712 588 354 167 99 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 111 1325 1671 1956 Size: 111 1214 346 285 Fragments arranged by size: 1214 346 285 111 BsiHKAI G_wGCw'C Cuts at: 0 928 1258 1328 1956 Size: 928 330 70 628 Fragments arranged by size: 928 628 330 70 BsmI GAATG_Cn' Cuts at: 0 306 1956 Size: 306 1650 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1101 1956 Size: 1101 855 Bsp1286I G_dGCh'C Cuts at: 0 94 928 1258 1328 1956 Size: 94 834 330 70 628 Fragments arranged by size: 834 628 330 94 70 BspHI T'CATG_A Cuts at: 0 1385 1956 Size: 1385 571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 139 1800 1956 Size: 139 1661 156 Fragments arranged by size: 1661 156 139 BsrDI GCAATG_nn' Cuts at: 0 10 707 1956 Size: 10 697 1249 Fragments arranged by size: 1249 697 10 BsrFI r'CCGG_y Cuts at: 0 1267 1956 Size: 1267 689 BsrGI T'GTAC_A Cuts at: 0 211 1956 Size: 211 1745 BssSI C'ACGA_G Cuts at: 0 1219 1956 Size: 1219 737 BstXI CCAn_nnnn'nTGG Cuts at: 0 1893 1956 Size: 1893 63 BstYI r'GATC_y Cuts at: 0 154 534 1056 1956 Size: 154 380 522 900 Fragments arranged by size: 900 522 380 154 BtgI C'CryG_G Cuts at: 0 138 1956 Size: 138 1818 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1248 1956 Size: 1248 708 BtsI GCAGTG_nn' Cuts at: 0 123 675 1168 1337 1956 Size: 123 552 493 169 619 Fragments arranged by size: 619 552 493 169 123 ClaI AT'CG_AT Cuts at: 0 1482 1956 Size: 1482 474 DraIII CAC_nnn'GTG Cuts at: 0 995 1694 1956 Size: 995 699 262 Fragments arranged by size: 995 699 262 EaeI y'GGCC_r Cuts at: 0 1292 1436 1956 Size: 1292 144 520 Fragments arranged by size: 1292 520 144 EarI CTCTTCn'nnn_ Cuts at: 0 758 916 1201 1219 1336 1678 1750 1939 Size: 758 158 285 18 117 342 72 189 Cuts at: 1939 1956 Size: 17 Fragments arranged by size: 758 342 285 189 158 117 72 18 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 632 1336 1956 Size: 632 704 620 Fragments arranged by size: 704 632 620 EcoNI CCTnn'n_nnAGG Cuts at: 0 1501 1956 Size: 1501 455 EcoO109I rG'GnC_Cy Cuts at: 0 697 1897 1956 Size: 697 1200 59 Fragments arranged by size: 1200 697 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 346 378 452 484 490 522 592 624 Size: 346 32 74 32 6 32 70 32 Cuts at: 624 1475 1507 1956 Size: 851 32 449 Fragments arranged by size: 851 449 346 74 70 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 0 1362 1625 1956 Size: 1362 263 331 Fragments arranged by size: 1362 331 263 MfeI C'AATT_G Cuts at: 0 603 1956 Size: 603 1353 MscI TGG'CCA Cuts at: 0 1438 1956 Size: 1438 518 MslI CAynn'nnrTG Cuts at: 0 1081 1306 1466 1642 1956 Size: 1081 225 160 176 314 Fragments arranged by size: 1081 314 225 176 160 MspA1I CmG'CkG Cuts at: 0 1426 1723 1956 Size: 1426 297 233 Fragments arranged by size: 1426 297 233 NcoI C'CATG_G Cuts at: 0 138 1956 Size: 138 1818 NspI r_CATG'y Cuts at: 0 194 1956 Size: 194 1762 PasI CC'CwG_GG Cuts at: 0 378 1956 Size: 378 1578 PciI A'CATG_T Cuts at: 0 190 1956 Size: 190 1766 PflMI CCAn_nnn'nTGG Cuts at: 0 1734 1956 Size: 1734 222 PfoI T'CCnGG_A Cuts at: 0 494 1932 1956 Size: 494 1438 24 Fragments arranged by size: 1438 494 24 PmlI CAC'GTG Cuts at: 0 876 975 1956 Size: 876 99 981 Fragments arranged by size: 981 876 99 PpuMI rG'GwC_Cy Cuts at: 0 697 1897 1956 Size: 697 1200 59 Fragments arranged by size: 1200 697 59 PshAI GACnn'nnGTC Cuts at: 0 1357 1956 Size: 1357 599 PstI C_TGCA'G Cuts at: 0 1181 1449 1956 Size: 1181 268 507 Fragments arranged by size: 1181 507 268 PvuII CAG'CTG Cuts at: 0 1723 1956 Size: 1723 233 SapI GCTCTTCn'nnn_ Cuts at: 0 916 1678 1956 Size: 916 762 278 Fragments arranged by size: 916 762 278 SfcI C'TryA_G Cuts at: 0 222 1177 1445 1708 1956 Size: 222 955 268 263 248 Fragments arranged by size: 955 268 263 248 222 SgrAI Cr'CCGG_yG Cuts at: 0 1267 1956 Size: 1267 689 SmlI C'TyrA_G Cuts at: 0 587 1368 1956 Size: 587 781 588 Fragments arranged by size: 781 588 587 StuI AGG'CCT Cuts at: 0 26 1956 Size: 26 1930 StyI C'CwwG_G Cuts at: 0 138 182 1244 1524 1767 1956 Size: 138 44 1062 280 243 189 Fragments arranged by size: 1062 280 243 189 138 44 TatI w'GTAC_w Cuts at: 0 211 1956 Size: 211 1745 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 681 713 1956 Size: 681 32 1243 Fragments arranged by size: 1243 681 32 XbaI T'CTAG_A Cuts at: 0 516 1956 Size: 516 1440 XmnI GAAnn'nnTTC Cuts at: 0 169 1599 1956 Size: 169 1430 357 Fragments arranged by size: 1430 357 169 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AloI ApaLI ApoI AseI BamHI BbsI BbvCI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI ClaI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII MfeI MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PstI PvuII SapI SfcI SgrAI SmlI StuI StyI TatI TstI XbaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI BanI BanII BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BspEI BsrBI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XcmI XhoI XmaI ZraI