################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BanI BanII BbeI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsoBI BspEI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Alw44I 1324 GTGCAC G^TGCAC ApaLI 1324 GTGCAC G^TGCAC AseI 202 ATTAAT AT^TAAT AsnI 202 ATTAAT AT^TAAT BalI 1436 TGGCCA TGG^CCA BamHI 1056 GGATCC G^GATCC BbsI 904 GTCTTC GAAGAC(2/6) BbvCI 1800 CCTCAGC CCTCAGC(-5/-2) BcnI 495 CCGGG CC^[GC]GG BfaI 517 CTAG C^TAG BglI 1763 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1315 CTGGAG CTGGAG(16/14) BpuAI 904 GTCTTC GAAGAC(2/6) BsmBI 1095 CGTCTC CGTCTC(1/5) BsmI 306 GCATTC GAATGC(1/-1) Bsp1407I 211 TGTACA T^GTACA BspDI 1481 ATCGAT AT^CGAT BspHI 1385 TCATGA T^CATGA BspLU11I 190 ACATGT A^CATGT BsrFI 1267 GCCGGT [GA]^CCGG[CT] BsrGI 211 TGTACA T^GTACA BssSI 1219 CTCGTG CACGAG(-5/-1) BstXI 1886 CCAGCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1267 GCCGGT [GA]^CCGG[CT] ClaI 1481 ATCGAT AT^CGAT DsaI 138 CCATGG C^C[GA][CT]GG EcoNI 1497 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 1423 CCCGC CCCGC(4/6) MaeI 517 CTAG C^TAG MfeI 603 CAATTG C^AATTG MluNI 1436 TGGCCA TGG^CCA MscI 1436 TGGCCA TGG^CCA MunI 603 CAATTG C^AATTG NciI 495 CCGGG CC^[GC]GG NcoI 138 CCATGG C^CATGG NspI 190 ACATGT [GA]CATG^[CT] PflMI 1728 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1353 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 202 ATTAAT AT^TAAT PvuII 1721 CAGCTG CAG^CTG RcaI 1385 TCATGA T^CATGA SgrAI 1266 CGCCGGTG C[GA]^CCGG[CT]G SspBI 211 TGTACA T^GTACA StuI 24 AGGCCT AGG^CCT Van91I 1728 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 516 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 913 GTCTAC GT^[AC][GT]AC 1789 GTCTAC AcsI 412 AAATTT [GA]^AATT[CT] 1554 GAATTT AfaI 212 GTAC GT^AC 893 GTAC ApoI 412 AAATTT [GA]^AATT[CT] 1554 GAATTT Asp700I 165 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1595 GAAAACTTTC BbrPI 874 CACGTG CAC^GTG 973 CACGTG BspMI 130 ACCTGC ACCTGC(4/8) 1809 GCAGGT BsrDI 11 CATTGC GCAATG(2/0) 708 CATTGC DraII 696 AGGTCCC [GA]G^G[ACGT]CC[CT] 1896 GGGTCCT DraIII 990 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 1689 CACATGGTG EaeI 1292 TGGCCG [CT]^GGCC[GA] 1436 TGGCCA EcoO109I 696 AGGTCCC [GA]G^G[ACGT]CC[CT] 1896 GGGTCCT HapII 495 CCGG C^CGG 1268 CCGG HgaI 858 GACGC GACGC(5/10) 1094 GCGTC HincII 1360 GTCAAC GT[CT]^[GA]AC 1623 GTCAAC HindII 1360 GTCAAC GT[CT]^[GA]AC 1623 GTCAAC HpaII 495 CCGG C^CGG 1268 CCGG MseI 203 TTAA T^TAA 242 TTAA MspA1I 1424 CCGCTG C[AC]G^C[GT]G 1721 CAGCTG MspI 495 CCGG C^CGG 1268 CCGG PmaCI 874 CACGTG CAC^GTG 973 CACGTG PmlI 874 CACGTG CAC^GTG 973 CACGTG PpuMI 696 AGGTCCC [GA]G^G[AT]CC[CT] 1896 GGGTCCT PstI 1177 CTGCAG CTGCA^G 1445 CTGCAG RsaI 212 GTAC GT^AC 893 GTAC SapI 921 GAAGAGC GCTCTTC(1/4) 1671 GCTCTTC SmlI 587 CTCAAG C^T[CT][GA]AG 1368 CTCAAG TfiI 505 GAATC G^A[AT]TC 1508 GATTC Tru9I 203 TTAA T^TAA 242 TTAA XmnI 165 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1595 GAAAACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AflIII AluI AlwI AspHI AvaII BbvI BlpI BmyI Bpu1102I BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII