SHC4 ORF clone FHC25382

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC25382

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Clone info.

Species

human

Clone Name

pFN21AE3764 Sequence (with vector) Restriction Map

Product ID

FHC25382

ORF Sequence

DNA sequence (1890 bp)
Protein sequence (630 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3764

Symbol

SHC4

Alias : RaLP, SHCD

Description

SHC (Src homology 2 domain containing) family, member 4

Original Clone ID

cp93764

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1890 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 630 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10130.1 1.8e-170 100.0 SHC4 CCDS44233.1 8.2e-58 49.1 SHC1 CCDS30881.1 1.7e-57 49.0 SHC1 CCDS1076.1 3.7e-54 55.8 SHC1 CCDS44234.1 7.9e-54 55.7 SHC1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR006019 187 202 PR00629 Phosphotyrosine interaction (PID/PI) IPR006019 205 227 PR00629 Phosphotyrosine interaction (PID/PI) IPR006019 230 241 PR00629 Phosphotyrosine interaction (PID/PI) IPR006019 258 267 PR00629 Phosphotyrosine interaction (PID/PI) IPR006019 281 309 PR00629 Phosphotyrosine interaction (PID/PI) IPR006019 316 342 PR00629 Phosphotyrosine interaction (PID/PI) IPR000980 526 540 PR00401 SH2 motif IPR000980 543 553 PR00401 SH2 motif IPR000980 555 566 PR00401 SH2 motif IPR000980 586 600 PR00401 SH2 motif HMMPfam IPR006020 192 347 PF00640 Phosphotyrosine interaction domain IPR000980 527 597 PF00017 SH2 motif HMMSmart IPR006020 187 351 SM00462 Phosphotyrosine interaction domain IPR000980 524 603 SM00252 SH2 motif ProfileScan IPR006020 189 349 PS01179 Phosphotyrosine interaction domain IPR000980 526 617 PS50001 SH2 motif
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001123512 584 600560 49.1 94.1 NP_892113 583 600560 49.0 94.1 XP_011508194 593 600560 48.5 94.1 XP_011508195 592 600560 48.5 94.1 XP_005245506 598 600560 47.6 87.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3764 Download

>KIEE3764  1890 bp
ATGCGAGAACGCGGCCAGGACAGCCTGGCAGGACTCGTGCTGTATGTAGGACTCTTCGGG
CACCCCGGGATGCTGCACAGGGCCAAGTACAGCCGCTTTCGGAACGAGTCGATCACGTCC
TTGGACGAAGGTAGCTCCGGAGGCTCGGTCGGGAACAAGGGCTCGCCGCAGCCTCCCCAC
CCCGCCCTGGCACCTCACCTGCCGACTGAAGATGCCACCTTGCCGTCGCAGGAGAGCCCC
ACCCCACTGTGCACCTTGATCCCCCGCATGGCAAGCATGAAGCTGGCCAACCCGGCCACT
TTGCTGAGTCTGAAAAACTTTTGCCTGGGTACCAAAGAGGTGCCTCGGCTGAAGCTCCAG
GAAAGCCGGGACCCAGGTTCCAGCGGCCCCTCTTCCCCAGAAACCAGTTTAAGTAGGTCC
GGGACTGCACCTCCACCGCAGCAGGACCTGGTGGGACACAGGGCAACCGCCCTAACCCCT
GATTCGTGCCCGCTTCCTGGCCCTGGGGAGCCAACACTTAGGAGCAGGCAGGACAGGCAC
TTTCTACAGCACCTGTTGGGGATGGGCATGAACTACTGTGTGAGGTACATGGGCTGTGTT
GAAGTGCTGCAATCAATGAGATCACTGGATTTTGGAATGAGAACCCAAGTTACAAGGGAA
GCAATAAGTCGCCTGTGTGAAGCTGTCCCCGGGGCAAATGGAGCCATTAAAAAGCGAAAG
CCTCCAGTTAAGTTCCTATCAACAGTCCTTGGCAAAAGTAATCTTCAGTTTTCAGGAATG
AATATAAAACTGACCATCTCAACATGCAGTCTCACATTGATGAATCTTGACAACCAACAG
ATTATTGCAAATCATCATATGCAGTCTATTTCATTTGCCTCTGGAGGGGATCCTGATACT
ACAGACTATGTTGCCTACGTAGCTAAAGATCCAGTTAATCAACGAGCCTGTCACATATTG
GAATGCCACAATGGAATGGCCCAAGACGTCATAAGTACCATAGGGCAGGCTTTTGAACTC
CGGTTCAAACAGTACTTGAAAAATCCTTCTTTGAATACTTCTTGTGAAAGTGAGGAGGTG
CATATTGATAGCCATGCCGAGGAGAGAGAAGATCATGAATATTACAATGAAATTCCAGGG
AAGCAGCCACCAGTAGGTGGTGTTTCAGATATGCGGATCAAAGTTCAAGCCACGGAACAA
ATGGCTTACTGCCCCATACAGTGTGAAAAGTTGTGCTATTTGCCTGGAAACTCCAAGTGC
AGCAGTGTATATGAGAACTGTTTAGAACAAAGCAGGGCAATAGGTAATGTCCATCCAAGA
GGGGTGCAGTCCCAGCGAGATACCTCATTATTGAAGCACACGTGCCGAGTGGATCTCTTT
GATGACCCCTGCTACATTAATACACAGGCTCTTCAAAGTACACCTGGCTCTGCTGGAAAT
CAAAGGTCAGCCCAACCACTGGGGAGCCCATGGCACTGCGGAAAGGCACCAGAAACTGTT
CAGCCGGGTGCCACAGCCCAGCCTGCCAGCTCACATTCTTTGCCACACATTAAGCAGCAG
CTGTGGAGCGAAGAATGCTATCATGGCAAGCTGAGCAGGAAGGCGGCAGAGAGCCTCTTG
GTAAAGGATGGGGACTTTTTGGTTCGAGAGAGTGCAACATCCCCTGGCCAATATGTGCTG
AGTGGACTACAGGGAGGCCAAGCAAAACATCTTCTCCTGGTGGATCCTGAAGGCAAGGTG
AGGACCAAGGATCATGTATTTGATAATGTCGGCCACCTTATCAGATACCATATGGATAAC
AGTTTGCCAATCATCTCCTCTGGAAGCGAAGTAAGCCTTAAACAACCAGTGAGAAAAGAT
AATAATCCAGCACTTTTGCATTCCAACAAA

ORF Protein

Cloned ORF protein sequence for pFN21AE3764 Download

>KIEE3764  630 aa
MRERGQDSLAGLVLYVGLFGHPGMLHRAKYSRFRNESITSLDEGSSGGSVGNKGSPQPPH
PALAPHLPTEDATLPSQESPTPLCTLIPRMASMKLANPATLLSLKNFCLGTKEVPRLKLQ
ESRDPGSSGPSSPETSLSRSGTAPPPQQDLVGHRATALTPDSCPLPGPGEPTLRSRQDRH
FLQHLLGMGMNYCVRYMGCVEVLQSMRSLDFGMRTQVTREAISRLCEAVPGANGAIKKRK
PPVKFLSTVLGKSNLQFSGMNIKLTISTCSLTLMNLDNQQIIANHHMQSISFASGGDPDT
TDYVAYVAKDPVNQRACHILECHNGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEV
HIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKC
SSVYENCLEQSRAIGNVHPRGVQSQRDTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGN
QRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLL
VKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDN
SLPIISSGSEVSLKQPVRKDNNPALLHSNK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3764 Download

>pFN21AE3764  6595 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCGAGAACGCGGCCAGGACAGCCTGGCAGGACT
CGTGCTGTATGTAGGACTCTTCGGGCACCCCGGGATGCTGCACAGGGCCAAGTACAGCCG
CTTTCGGAACGAGTCGATCACGTCCTTGGACGAAGGTAGCTCCGGAGGCTCGGTCGGGAA
CAAGGGCTCGCCGCAGCCTCCCCACCCCGCCCTGGCACCTCACCTGCCGACTGAAGATGC
CACCTTGCCGTCGCAGGAGAGCCCCACCCCACTGTGCACCTTGATCCCCCGCATGGCAAG
CATGAAGCTGGCCAACCCGGCCACTTTGCTGAGTCTGAAAAACTTTTGCCTGGGTACCAA
AGAGGTGCCTCGGCTGAAGCTCCAGGAAAGCCGGGACCCAGGTTCCAGCGGCCCCTCTTC
CCCAGAAACCAGTTTAAGTAGGTCCGGGACTGCACCTCCACCGCAGCAGGACCTGGTGGG
ACACAGGGCAACCGCCCTAACCCCTGATTCGTGCCCGCTTCCTGGCCCTGGGGAGCCAAC
ACTTAGGAGCAGGCAGGACAGGCACTTTCTACAGCACCTGTTGGGGATGGGCATGAACTA
CTGTGTGAGGTACATGGGCTGTGTTGAAGTGCTGCAATCAATGAGATCACTGGATTTTGG
AATGAGAACCCAAGTTACAAGGGAAGCAATAAGTCGCCTGTGTGAAGCTGTCCCCGGGGC
AAATGGAGCCATTAAAAAGCGAAAGCCTCCAGTTAAGTTCCTATCAACAGTCCTTGGCAA
AAGTAATCTTCAGTTTTCAGGAATGAATATAAAACTGACCATCTCAACATGCAGTCTCAC
ATTGATGAATCTTGACAACCAACAGATTATTGCAAATCATCATATGCAGTCTATTTCATT
TGCCTCTGGAGGGGATCCTGATACTACAGACTATGTTGCCTACGTAGCTAAAGATCCAGT
TAATCAACGAGCCTGTCACATATTGGAATGCCACAATGGAATGGCCCAAGACGTCATAAG
TACCATAGGGCAGGCTTTTGAACTCCGGTTCAAACAGTACTTGAAAAATCCTTCTTTGAA
TACTTCTTGTGAAAGTGAGGAGGTGCATATTGATAGCCATGCCGAGGAGAGAGAAGATCA
TGAATATTACAATGAAATTCCAGGGAAGCAGCCACCAGTAGGTGGTGTTTCAGATATGCG
GATCAAAGTTCAAGCCACGGAACAAATGGCTTACTGCCCCATACAGTGTGAAAAGTTGTG
CTATTTGCCTGGAAACTCCAAGTGCAGCAGTGTATATGAGAACTGTTTAGAACAAAGCAG
GGCAATAGGTAATGTCCATCCAAGAGGGGTGCAGTCCCAGCGAGATACCTCATTATTGAA
GCACACGTGCCGAGTGGATCTCTTTGATGACCCCTGCTACATTAATACACAGGCTCTTCA
AAGTACACCTGGCTCTGCTGGAAATCAAAGGTCAGCCCAACCACTGGGGAGCCCATGGCA
CTGCGGAAAGGCACCAGAAACTGTTCAGCCGGGTGCCACAGCCCAGCCTGCCAGCTCACA
TTCTTTGCCACACATTAAGCAGCAGCTGTGGAGCGAAGAATGCTATCATGGCAAGCTGAG
CAGGAAGGCGGCAGAGAGCCTCTTGGTAAAGGATGGGGACTTTTTGGTTCGAGAGAGTGC
AACATCCCCTGGCCAATATGTGCTGAGTGGACTACAGGGAGGCCAAGCAAAACATCTTCT
CCTGGTGGATCCTGAAGGCAAGGTGAGGACCAAGGATCATGTATTTGATAATGTCGGCCA
CCTTATCAGATACCATATGGATAACAGTTTGCCAATCATCTCCTCTGGAAGCGAAGTAAG
CCTTAAACAACCAGTGAGAAAAGATAATAATCCAGCACTTTTGCATTCCAACAAAGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT