(Circular) (Six-base) MAPSORT of: pFN21AE3764.seq Check: 573 from: 1 to: 6595 pFN21AE3764 6595 bp With 182 enzymes: SgfI * March 28, 2012 12:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2211 2211 Size: 6595 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2994 4405 278 Size: 53 83 186 936 1458 1411 2468 Fragments arranged by size: 2468 1458 1411 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3938 1937 Size: 2001 4594 Acc65I G'GTAC_C Cuts at: 2333 3916 2333 Size: 1583 5012 AclI AA'CG_TT Cuts at: 1563 4722 5095 1563 Size: 3159 373 3063 Fragments arranged by size: 3159 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 2375 2753 3753 4658 6042 6310 1632 Size: 600 143 378 1000 905 1384 268 1917 Fragments arranged by size: 1917 1384 1000 905 600 378 268 143 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6398 AflIII A'CryG_T Cuts at: 1203 3363 5494 6471 1203 Size: 2160 2131 977 1327 Fragments arranged by size: 2160 2131 1327 977 AgeI A'CCGG_T Cuts at: 5426 5426 Size: 6595 AhdI GACnn_n'nnGTC Cuts at: 5324 5324 Size: 6595 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6595 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6595 AlwNI CAG_nnn'CTG Cuts at: 2452 2557 3500 3565 4338 5910 2452 Size: 105 943 65 773 1572 3137 Fragments arranged by size: 3137 1572 943 773 105 65 ApaLI G'TGCA_C Cuts at: 2254 4652 5808 2254 Size: 2398 1156 3041 Fragments arranged by size: 3041 2398 1156 ApoI r'AATT_y Cuts at: 1493 3135 3904 4130 4282 6391 1493 Size: 1642 769 226 152 2109 1697 Fragments arranged by size: 2109 1697 1642 769 226 152 AseI AT'TA_AT Cuts at: 160 1032 3402 5149 160 Size: 872 2370 1747 1606 Fragments arranged by size: 2370 1747 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2693 3920 1870 Size: 73 126 624 1227 4545 Fragments arranged by size: 4545 1227 624 126 73 BamHI G'GATC_C Cuts at: 2893 3727 3925 4292 2893 Size: 834 198 367 5196 Fragments arranged by size: 5196 834 367 198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2064 2194 2333 2344 3490 Size: 325 217 627 277 130 139 11 1146 Cuts at: 3490 3512 3916 5371 618 Size: 22 404 1455 1842 Fragments arranged by size: 1842 1455 1146 627 404 325 277 217 139 130 22 11 BanII G_rGCy'C Cuts at: 1391 2168 2243 3473 3914 1391 Size: 777 75 1230 441 4072 Fragments arranged by size: 4072 1230 777 441 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5624 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4786 4820 1903 Size: 34 50 34 2765 34 3678 Fragments arranged by size: 3678 2765 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4487 5697 4487 Size: 1210 5385 BclI T'GATC_A Cuts at: 1548 1548 Size: 6595 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2297 5206 136 Size: 107 122 71 631 605 625 2909 1525 Fragments arranged by size: 2909 1525 631 625 605 122 107 71 BglII A'GATC_T Cuts at: 6590 6590 Size: 6595 BlpI GC'TnA_GC Cuts at: 3596 4012 3596 Size: 416 6179 Bme1580I G_kGCm'C Cuts at: 1790 2067 2258 2495 4656 5812 1790 Size: 277 191 237 2161 1156 2573 Fragments arranged by size: 2573 2161 1156 277 237 191 BmgBI CAC'GTC Cuts at: 1318 2121 1318 Size: 803 5792 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3473 4326 5284 Size: 416 53 83 399 24 2056 853 958 Cuts at: 5284 442 Size: 1753 Fragments arranged by size: 2056 1753 958 853 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6595 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3395 3395 Size: 6595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2712 2907 5255 1239 Size: 1106 367 195 2348 2579 Fragments arranged by size: 2579 2348 1106 367 195 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4720 5585 5883 4720 Size: 865 298 5432 Fragments arranged by size: 5432 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5258 882 Size: 617 3759 2219 Fragments arranged by size: 3759 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2923 3366 4386 493 Size: 711 1719 443 1020 2702 Fragments arranged by size: 2702 1719 1020 711 443 BsaBI GATnn'nnATC Cuts at: 3867 6589 3867 Size: 2722 3873 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2991 4402 4784 5419 Size: 53 83 186 936 1458 1411 382 635 Cuts at: 5419 275 Size: 1451 Fragments arranged by size: 1458 1451 1411 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2141 3024 5028 5426 5700 5847 6258 2141 Size: 883 2004 398 274 147 411 2478 Fragments arranged by size: 2478 2004 883 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2136 2166 2419 2449 3702 Size: 30 165 30 733 30 253 30 1253 Cuts at: 3702 3732 1178 Size: 30 4041 Fragments arranged by size: 4041 1253 733 253 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3092 3119 3811 1186 Size: 1906 27 692 3970 Fragments arranged by size: 3970 1906 692 27 BseYI C'CCAG_C Cuts at: 3336 3522 5798 6294 3336 Size: 186 2276 496 3637 Fragments arranged by size: 3637 2276 496 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 2415 3283 3350 1163 Size: 573 327 352 868 67 4408 Fragments arranged by size: 4408 868 573 352 327 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2154 4045 4806 5834 1540 Size: 275 186 153 1891 761 1028 2301 Fragments arranged by size: 2301 1891 1028 761 275 186 153 BsiHKAI G_wGCw'C Cuts at: 2258 4656 4741 5812 2258 Size: 2398 85 1071 3041 Fragments arranged by size: 3041 2398 1071 85 BsmI GAATG_Cn' Cuts at: 2972 3584 3883 4106 4199 2972 Size: 612 299 223 93 5368 Fragments arranged by size: 5368 612 299 223 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2168 2243 2258 2495 3473 3914 Size: 399 277 101 75 15 237 978 441 Cuts at: 3914 4656 4741 5812 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 978 742 441 399 277 237 101 85 75 15 BspEI T'CCGG_A Cuts at: 2141 6258 2141 Size: 4117 2478 BspHI T'CATG_A Cuts at: 3118 4484 6214 3118 Size: 1366 1730 3499 Fragments arranged by size: 3499 1730 1366 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2211 3950 844 Size: 1367 1739 3489 Fragments arranged by size: 3489 1739 1367 BsrBI CCG'CTC Cuts at: 1394 1823 4482 1394 Size: 429 2659 3507 Fragments arranged by size: 3507 2659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5090 5264 65 Size: 1183 3842 174 1396 Fragments arranged by size: 3842 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5239 5426 1700 Size: 110 3429 187 2869 Fragments arranged by size: 3429 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6595 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6595 BssSI C'ACGA_G Cuts at: 2039 4655 5667 2039 Size: 2616 1012 2967 Fragments arranged by size: 2967 2616 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6595 BstBI TT'CG_AA Cuts at: 5399 5399 Size: 6595 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6595 BstYI r'GATC_y Cuts at: 1972 2893 2932 3376 3727 3925 4292 4677 4694 Size: 921 39 444 351 198 367 385 17 Cuts at: 4694 6146 6232 6244 6590 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 921 444 385 367 351 346 198 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 3195 3473 513 Size: 552 2130 278 3635 Fragments arranged by size: 3635 2130 552 278 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5856 Fragments arranged by size: 5856 683 56 BtsI GCAGTG_nn' Cuts at: 3274 3478 4111 4905 4925 3274 Size: 204 633 794 20 4944 Fragments arranged by size: 4944 794 633 204 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6595 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6560 DraI TTT'AAA Cuts at: 3899 3899 Size: 6595 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5602 809 Size: 507 4286 1802 Fragments arranged by size: 4286 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2017 2289 2298 Size: 54 1425 50 275 18 187 272 9 Cuts at: 2298 3670 3775 4042 4931 6560 8 Size: 1372 105 267 889 1629 43 Fragments arranged by size: 1629 1425 1372 889 275 272 267 187 105 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 4042 1537 Size: 275 2230 4090 Fragments arranged by size: 4090 2230 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2401 3420 4525 1353 Size: 710 338 1019 1105 3423 Fragments arranged by size: 3423 1105 1019 710 338 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5182 5556 5702 387 Size: 1064 138 3593 374 146 1280 Fragments arranged by size: 3593 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2345 2375 2712 2753 2907 3753 Size: 393 600 113 30 337 41 154 846 Cuts at: 3753 4658 5255 6042 6310 1239 Size: 905 597 787 268 1524 Fragments arranged by size: 1524 905 846 787 600 597 393 337 268 154 113 41 30 EcoO109I rG'GnC_Cy Cuts at: 2374 2449 2374 Size: 75 6520 EcoRI G'AATT_C Cuts at: 3904 4282 3904 Size: 378 6217 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3857 3857 Size: 6595 FspI TGC'GCA Cuts at: 5101 5101 Size: 6595 HaeII r_GCGC'y Cuts at: 1678 5742 1678 Size: 4064 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2136 2168 2419 2451 3100 Size: 32 135 32 564 32 251 32 649 Cuts at: 3100 3132 3739 3771 5236 5268 5310 5342 1373 Size: 32 607 32 1465 32 42 32 2626 Fragments arranged by size: 2626 1465 649 607 564 251 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3939 4185 669 Size: 988 281 2001 246 3079 Fragments arranged by size: 3079 2001 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6595 HpaI GTT'AAC Cuts at: 4185 4185 Size: 6595 KpnI G_GTAC'C Cuts at: 2337 3920 2337 Size: 1583 5012 MfeI C'AATT_G Cuts at: 4194 4194 Size: 6595 MluI A'CGCG_T Cuts at: 6471 6471 Size: 6595 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3912 5709 5893 1305 Size: 2607 1797 184 2007 Fragments arranged by size: 2607 2007 1797 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2291 3672 10 Size: 54 1425 343 459 1381 2933 Fragments arranged by size: 2933 1425 1381 459 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 4553 4912 5071 518 Size: 648 1656 1731 359 159 2042 Fragments arranged by size: 2042 1731 1656 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2388 3565 4011 4688 5836 6081 1606 Size: 782 1177 446 677 1148 245 2120 Fragments arranged by size: 2120 1177 1148 782 677 446 245 NcoI C'CATG_G Cuts at: 513 1065 3473 513 Size: 552 2408 3635 Fragments arranged by size: 3635 2408 552 NdeI CA'TA_TG Cuts at: 387 2862 3795 387 Size: 2475 933 3187 Fragments arranged by size: 3187 2475 933 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6595 NotI GC'GGCC_GC Cuts at: 4042 4042 Size: 6595 NspI r_CATG'y Cuts at: 1132 1229 2811 3953 5498 1132 Size: 97 1582 1142 1545 2229 Fragments arranged by size: 2229 1582 1545 1142 97 PasI CC'CwG_GG Cuts at: 1348 2507 1348 Size: 1159 5436 PciI A'CATG_T Cuts at: 5494 5494 Size: 6595 PflMI CCAn_nnn'nTGG Cuts at: 1108 3161 3463 1108 Size: 2053 302 4240 Fragments arranged by size: 4240 2053 302 PfoI T'CCnGG_A Cuts at: 2361 2423 2361 Size: 62 6533 PmeI GTTT'AAAC Cuts at: 3899 3899 Size: 6595 PmlI CAC'GTG Cuts at: 3366 3366 Size: 6595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2150 2182 4664 4696 6177 6209 2150 Size: 32 2482 32 1481 32 2536 Fragments arranged by size: 2536 2482 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2374 2449 2374 Size: 75 6520 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6595 PsiI TTA'TAA Cuts at: 4165 4165 Size: 6595 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2638 2670 2638 Size: 32 6563 PstI C_TGCA'G Cuts at: 830 3947 830 Size: 3117 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6595 PvuII CAG'CTG Cuts at: 3565 3565 Size: 6595 SalI G'TCGA_C Cuts at: 1936 3937 1936 Size: 2001 4594 SanDI GG'GwC_CC Cuts at: 2374 2374 Size: 6595 SapI GCTCTTCn'nnn_ Cuts at: 3420 3420 Size: 6595 SbfI CC_TGCA'GG Cuts at: 3947 3947 Size: 6595 ScaI AGT'ACT Cuts at: 3038 4843 6555 3038 Size: 1805 1712 3078 Fragments arranged by size: 3078 1805 1712 SexAI A'CCwGG_T Cuts at: 2451 2451 Size: 6595 SfcI C'TryA_G Cuts at: 826 1045 2549 2904 3692 3943 5078 5759 5950 Size: 219 1504 355 788 251 1135 681 191 Cuts at: 5950 826 Size: 1471 Fragments arranged by size: 1504 1471 1135 788 681 355 251 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2297 2297 Size: 6595 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6595 SmaI CCC'GGG Cuts at: 1872 2071 2695 3922 1872 Size: 199 624 1227 4545 Fragments arranged by size: 4545 1227 624 199 SmlI C'TyrA_G Cuts at: 820 1017 1943 4699 5600 5862 820 Size: 197 926 2756 901 262 1553 Fragments arranged by size: 2756 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2923 4386 493 Size: 2430 1463 2702 Fragments arranged by size: 2702 2430 1463 SpeI A'CTAG_T Cuts at: 152 152 Size: 6595 SphI G_CATG'C Cuts at: 1132 3953 1132 Size: 2821 3774 SspI AAT'ATT Cuts at: 5 52 3125 4519 5 Size: 47 3073 1394 2081 Fragments arranged by size: 3073 2081 1394 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6595 StyI C'CwwG_G Cuts at: 513 1065 2124 2752 3473 3750 4034 513 Size: 552 1059 628 721 277 284 3074 Fragments arranged by size: 3074 1059 721 628 552 284 277 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 4622 4639 4792 4977 5422 325 Size: 1255 560 2482 17 153 185 445 1498 Fragments arranged by size: 2482 1498 1255 560 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 3036 3422 Size: 274 80 33 51 1443 114 945 386 Cuts at: 3422 4841 6553 96 Size: 1419 1712 138 Fragments arranged by size: 1712 1443 1419 945 386 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6555 6587 6555 Size: 32 6563 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6595 XbaI T'CTAG_A Cuts at: 3931 3931 Size: 6595 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3202 3202 Size: 6595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6595 XmaI C'CCGG_G Cuts at: 1870 2069 2693 3920 1870 Size: 199 624 1227 4545 Fragments arranged by size: 4545 1227 624 199 XmnI GAAnn'nnTTC Cuts at: 3501 4724 3501 Size: 1223 5372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2992 4403 276 Size: 53 83 186 936 1458 1411 2468 Fragments arranged by size: 2468 1458 1411 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SfoI SrfI SwaI