################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AccI AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AspEI AspI AviII AvrII BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HindIII HpaI KasI KspI MaeI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI SfoI SfuI SgrAI SmlI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 985 GACGTC GACGT^C Acc65I 328 GGTACC G^GTACC AccII 10 CGCG CG^CG AccIII 136 TCCGGA T^CCGGA AcsI 1130 AAATTC [GA]^AATT[CT] AcyI 985 GACGTC G[GA]^CG[CT]C AflIII 1358 ACACGT A^C[GA][CT]GT Alw44I 249 GTGCAC G^TGCAC ApaLI 249 GTGCAC G^TGCAC ApoI 1130 AAATTC [GA]^AATT[CT] AseI 1396 ATTAAT AT^TAAT AsnI 1396 ATTAAT AT^TAAT Asp700I 1492 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 328 GGTACC G^GTACC AspHI 249 GTGCAC G[AT]GC[AT]^C BbrPI 1359 CACGTG CAC^GTG BglI 286 GCCAACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1590 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1590 GCTGAGC GC^T[ACGT]AGC BsaBI 1858 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 985 GACGTC G[GA]^CG[CT]C BseAI 136 TCCGGA T^CCGGA BsiEI 146 CGGTCG CG[GA][CT]^CG BsiHKAI 249 GTGCAC G[AT]GC[AT]^C BsmAI 809 GTCTC GTCTC(1/5) BspEI 136 TCCGGA T^CCGGA BspHI 1113 TCATGA T^CATGA BspMI 197 ACCTGC ACCTGC(4/8) BssSI 34 CTCGTG CACGAG(-5/-1) BstUI 10 CGCG CG^CG CelII 1590 GCTGAGC GC^T[ACGT]AGC Hin1I 985 GACGTC G[GA]^CG[CT]C KpnI 328 GGTACC GGTAC^C MamI 1858 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 136 TCCGGA T^CCGGA MslI 813 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 10 CGCG CG^CG NcoI 1468 CCATGG C^CATGG NspI 802 ACATGC [GA]CATG^[CT] PmaCI 1359 CACGTG CAC^GTG PmlI 1359 CACGTG CAC^GTG PshBI 1396 ATTAAT AT^TAAT PvuII 1558 CAGCTG CAG^CTG RcaI 1113 TCATGA T^CATGA SapI 1408 GCTCTTC GCTCTTC(1/4) ScaI 1031 AGTACT AGT^ACT SexAI 446 ACCTGGT A^CC[AT]GGT SfiI 285 GGCCAACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 916 TACGTA TAC^GTA SspI 1118 AATATT AAT^ATT Tsp45I 950 GTCAC ^GT[GC]AC Tsp509I 1131 AATT ^AATT XcmI 1190 CCACGGAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1492 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaI 64 CCCGGG C^[CT]CG[GA]G 688 CCCGGG BalI 284 TGGCCA TGG^CCA 1665 TGGCCA BamHI 888 GGATCC G^GATCC 1722 GGATCC BsaAI 916 TACGTA [CT]AC^GT[GA] 1359 CACGTG BsaWI 136 TCCGGA [AT]^CCGG[AT] 1019 TCCGGT BsoBI 64 CCCGGG C^[CT]CG[GA]G 688 CCCGGG DraII 368 GGGACCC [GA]G^G[ACGT]CC[CT] 443 AGGACCT DsaI 1190 CCACGG C^C[GA][CT]GG 1468 CCATGG EcoO109I 368 GGGACCC [GA]G^G[ACGT]CC[CT] 443 AGGACCT HphI 1737 GGTGA GGTGA(8/7) 195 TCACC MaeIII 649 GTTAC ^GT[ACGT]AC 950 GTCAC MluNI 284 TGGCCA TGG^CCA 1665 TGGCCA MscI 284 TGGCCA TGG^CCA 1665 TGGCCA MspA1I 381 CAGCGG C[AC]G^C[GT]G 1558 CAGCTG NdeI 856 CATATG CA^TATG 1789 CATATG PflMI 1150 CCAGTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1452 CCAACCACTGG PpuMI 368 GGGACCC [GA]G^G[AT]CC[CT] 443 AGGACCT SfaNI 69 GATGC GCATC(5/9) 211 GATGC SmaI 64 CCCGGG CCC^GGG 688 CCCGGG TaqI 109 TCGA T^CGA 1644 TCGA TfiI 481 GATTC G^A[AT]TC 822 GAATC TthHB8I 109 TCGA T^CGA 1644 TCGA Van91I 1150 CCAGTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1452 CCAACCACTGG XmaCI 64 CCCGGG C^CCGGG 688 CCCGGG XmaI 64 CCCGGG C^CCGGG 688 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI StyI Tru9I TseI TspRI XhoII