GPR176 ORF clone FHC25339

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC25339

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Clone info.

Species

human

Clone Name

pFN21AE9433 Sequence (with vector) Restriction Map

Product ID

FHC25339

ORF Sequence

DNA sequence (1545 bp)
Protein sequence (515 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9433

Symbol

GPR176

Alias : HB-954

Description

G protein-coupled receptor 176, transcript variant 1

Original Clone ID

cp99433

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1545 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 515 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10051.1 3e-160 100.0 GPR176 CCDS61589.1 3.2e-152 96.1 GPR176 CCDS61588.1 1.5e-142 98.7 GPR176 CCDS7606.1 1.9e-12 25.6 PRLHR The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 43 67 PR00237 GPCR IPR000276 76 97 PR00237 GPCR IPR000276 124 146 PR00237 GPCR IPR000276 158 179 PR00237 GPCR IPR000276 206 229 PR00237 GPCR IPR000276 264 288 PR00237 GPCR IPR000276 302 328 PR00237 GPCR HMMPfam IPR000276 58 319 PF00001 GPCR ProfileScan IPR017452 58 320 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 44 TTTVQVVIFIGSLLGNFMVLWST 66 PRIMARY 23 2 82 NLACSGICASLVCVPFDIILSTS 104 SECONDARY 23 3 113 TMLFCKVVKFLHKVFCSVTILSF 135 PRIMARY 23 4 161 ELVMYIWAHAVVASVPVFAVTNV 183 PRIMARY 23 5 206 VLVYNITTVIVPVVVVFLFLILI 228 PRIMARY 23 6 267 TLLSMVMVFILCSVPYATLVVYQ 289 PRIMARY 23 7 306 VWLPKVSLLANPVLFLTVNKSV 327 SECONDARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_009154 515 612183 100.0 100.0 NP_001258783 514 612183 96.1 100.0 NP_001258784 470 612183 98.7 90.5 XP_011519469 483 612183 99.6 89.5 XP_016877362 483 612183 99.6 89.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9433 Download

>KIEE9433  1545 bp
ATGGGACATAACGGGAGCTGGATCTCTCCAAATGCCAGCGAGCCGCACAACGCGTCCGGC
GCCGAGGCTGCGGGTGTGAACCGCAGCGCGCTCGGGGAGTTCGGCGAGGCGCAGCTGTAC
CGCCAGTTCACCACCACCGTGCAGGTCGTCATCTTCATAGGCTCGCTGCTCGGAAACTTC
ATGGTGTTATGGTCAACTTGCCGCACAACCGTGTTCAAATCTGTCACCAACAGGTTCATT
AAAAACCTGGCCTGCTCGGGGATTTGTGCCAGCCTGGTCTGTGTGCCCTTCGACATCATC
CTCAGCACCAGTCCTCACTGTTGCTGGTGGATCTACACCATGCTCTTCTGCAAGGTCGTC
AAATTTTTGCACAAAGTATTCTGCTCTGTGACCATCCTCAGCTTCCCTGCTATTGCTTTG
GACAGGTACTACTCAGTCCTCTATCCACTGGAGAGGAAAATATCTGATGCCAAGTCCCGT
GAACTGGTGATGTACATCTGGGCCCATGCAGTGGTGGCCAGTGTCCCTGTGTTTGCAGTA
ACCAATGTGGCTGACATCTATGCCACGTCCACCTGCACGGAAGTCTGGAGCAACTCCTTG
GGCCACCTGGTGTACGTTCTGGTGTATAACATCACCACGGTCATTGTGCCTGTGGTGGTG
GTGTTCCTCTTCTTGATACTGATCCGACGGGCCCTGAGTGCCAGCCAGAAGAAGAAGGTC
ATCATAGCAGCGCTCCGGACCCCACAGAACACCATCTCTATTCCCTATGCCTCCCAGCGG
GAGGCCGAGCTGCACGCCACCCTGCTCTCCATGGTGATGGTCTTCATCTTGTGTAGCGTG
CCCTATGCCACCCTGGTCGTCTACCAGACTGTGCTCAATGTCCCTGACACTTCCGTCTTC
TTGCTGCTCACTGCTGTTTGGCTGCCCAAAGTCTCCCTGCTGGCAAACCCTGTTCTCTTT
CTTACTGTGAACAAATCTGTCCGCAAGTGCTTGATAGGGACCCTGGTGCAACTACACCAC
CGGTACAGTCGCCGTAATGTGGTCAGTACAGGGAGTGGCATGGCTGAGGCCAGCCTGGAA
CCCAGCATACGCTCGGGTAGCCAGCTCCTGGAGATGTTCCACATTGGGCAGCAGCAGATC
TTTAAGCCCACAGAGGATGAGGAAGAGAGTGAGGCCAAGTACATTGGCTCAGCTGACTTC
CAGGCCAAGGAGATATTTAGCACCTGCCTGGAGGGAGAGCAGGGGCCACAGTTTGCGCCC
TCTGCCCCACCCCTGAGCACAGTGGACTCTGTATCCCAGGTGGCACCGGCAGCCCCTGTG
GAACCTGAAACATTCCCTGATAAGTATTCCCTGCAGTTTGGCTTTGGGCCTTTTGAGTTG
CCTCCTCAGTGGCTCTCAGAGACCCGAAACAGCAAGAAGCGGCTGCTTCCCCCCTTGGGC
AACACCCCAGAAGAGCTGATCCAGACAAAGGTGCCCAAGGTAGGCAGGGTGGAGCGGAAG
ATGAGCAGAAACAATAAAGTGAGCATTTTTCCAAAGGTGGATTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE9433 Download

>KIEE9433  515 aa
MGHNGSWISPNASEPHNASGAEAAGVNRSALGEFGEAQLYRQFTTTVQVVIFIGSLLGNF
MVLWSTCRTTVFKSVTNRFIKNLACSGICASLVCVPFDIILSTSPHCCWWIYTMLFCKVV
KFLHKVFCSVTILSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAV
TNVADIYATSTCTEVWSNSLGHLVYVLVYNITTVIVPVVVVFLFLILIRRALSASQKKKV
IIAALRTPQNTISIPYASQREAELHATLLSMVMVFILCSVPYATLVVYQTVLNVPDTSVF
LLLTAVWLPKVSLLANPVLFLTVNKSVRKCLIGTLVQLHHRYSRRNVVSTGSGMAEASLE
PSIRSGSQLLEMFHIGQQQIFKPTEDEEESEAKYIGSADFQAKEIFSTCLEGEQGPQFAP
SAPPLSTVDSVSQVAPAAPVEPETFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLG
NTPEELIQTKVPKVGRVERKMSRNNKVSIFPKVDS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9433 Download

>pFN21AE9433  6250 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGACATAACGGGAGCTGGATCTCTCCAAATGC
CAGCGAGCCGCACAACGCGTCCGGCGCCGAGGCTGCGGGTGTGAACCGCAGCGCGCTCGG
GGAGTTCGGCGAGGCGCAGCTGTACCGCCAGTTCACCACCACCGTGCAGGTCGTCATCTT
CATAGGCTCGCTGCTCGGAAACTTCATGGTGTTATGGTCAACTTGCCGCACAACCGTGTT
CAAATCTGTCACCAACAGGTTCATTAAAAACCTGGCCTGCTCGGGGATTTGTGCCAGCCT
GGTCTGTGTGCCCTTCGACATCATCCTCAGCACCAGTCCTCACTGTTGCTGGTGGATCTA
CACCATGCTCTTCTGCAAGGTCGTCAAATTTTTGCACAAAGTATTCTGCTCTGTGACCAT
CCTCAGCTTCCCTGCTATTGCTTTGGACAGGTACTACTCAGTCCTCTATCCACTGGAGAG
GAAAATATCTGATGCCAAGTCCCGTGAACTGGTGATGTACATCTGGGCCCATGCAGTGGT
GGCCAGTGTCCCTGTGTTTGCAGTAACCAATGTGGCTGACATCTATGCCACGTCCACCTG
CACGGAAGTCTGGAGCAACTCCTTGGGCCACCTGGTGTACGTTCTGGTGTATAACATCAC
CACGGTCATTGTGCCTGTGGTGGTGGTGTTCCTCTTCTTGATACTGATCCGACGGGCCCT
GAGTGCCAGCCAGAAGAAGAAGGTCATCATAGCAGCGCTCCGGACCCCACAGAACACCAT
CTCTATTCCCTATGCCTCCCAGCGGGAGGCCGAGCTGCACGCCACCCTGCTCTCCATGGT
GATGGTCTTCATCTTGTGTAGCGTGCCCTATGCCACCCTGGTCGTCTACCAGACTGTGCT
CAATGTCCCTGACACTTCCGTCTTCTTGCTGCTCACTGCTGTTTGGCTGCCCAAAGTCTC
CCTGCTGGCAAACCCTGTTCTCTTTCTTACTGTGAACAAATCTGTCCGCAAGTGCTTGAT
AGGGACCCTGGTGCAACTACACCACCGGTACAGTCGCCGTAATGTGGTCAGTACAGGGAG
TGGCATGGCTGAGGCCAGCCTGGAACCCAGCATACGCTCGGGTAGCCAGCTCCTGGAGAT
GTTCCACATTGGGCAGCAGCAGATCTTTAAGCCCACAGAGGATGAGGAAGAGAGTGAGGC
CAAGTACATTGGCTCAGCTGACTTCCAGGCCAAGGAGATATTTAGCACCTGCCTGGAGGG
AGAGCAGGGGCCACAGTTTGCGCCCTCTGCCCCACCCCTGAGCACAGTGGACTCTGTATC
CCAGGTGGCACCGGCAGCCCCTGTGGAACCTGAAACATTCCCTGATAAGTATTCCCTGCA
GTTTGGCTTTGGGCCTTTTGAGTTGCCTCCTCAGTGGCTCTCAGAGACCCGAAACAGCAA
GAAGCGGCTGCTTCCCCCCTTGGGCAACACCCCAGAAGAGCTGATCCAGACAAAGGTGCC
CAAGGTAGGCAGGGTGGAGCGGAAGATGAGCAGAAACAATAAAGTGAGCATTTTTCCAAA
GGTGGATTCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT