################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AclI AflII AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BclI BcnI BfaI BfrI BglI BlnI BsaAI BsiEI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrDI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MfeI MunI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 859 GTCTAC GT^[AC][GT]AC AccIII 734 TCCGGA T^CCGGA AcsI 361 AAATTT [GA]^AATT[CT] AcyI 58 GGCGCC G[GA]^CG[CT]C AflIII 50 ACGCGT A^C[GA][CT]GT AgeI 1019 ACCGGT A^CCGGT Aor51HI 729 AGCGCT AGC^GCT ApoI 361 AAATTT [GA]^AATT[CT] BalI 515 TGGCCA TGG^CCA BbeI 58 GGCGCC GGCGC^C BcgI 83 GCAGCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1291 GTATCC GTATCC(6/5) BglII 1136 AGATCT A^GATCT BlpI 1187 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1187 GCTCAGC GC^T[ACGT]AGC BsaHI 58 GGCGCC G[GA]^CG[CT]C BsaI 1399 GAGACC GGTCTC(1/5) BseAI 734 TCCGGA T^CCGGA BseRI 1382 CTCCTC GAGGAG(10/8) Bsp1407I 491 TGTACA T^GTACA BspEI 734 TCCGGA T^CCGGA BsrBI 1492 GAGCGG CCGCTC(-3/-3) BsrGI 491 TGTACA T^GTACA BssHII 86 GCGCGC G^CGCGC BstXI 809 CCATGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1187 GCTCAGC GC^T[ACGT]AGC DraIII 604 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 886 GACACTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 515 TGGCCA [CT]^GGCC[GA] Eco47III 729 AGCGCT AGC^GCT HgaI 52 GCGTC GACGC(5/10) Hin1I 58 GGCGCC G[GA]^CG[CT]C HincII 192 GTCAAC GT[CT]^[GA]AC HindII 192 GTCAAC GT[CT]^[GA]AC KasI 58 GGCGCC G^GCGCC MluI 50 ACGCGT A^CGCGT MluNI 515 TGGCCA TGG^CCA MroI 734 TCCGGA T^CCGGA MscI 515 TGGCCA TGG^CCA NarI 58 GGCGCC GG^CGCC NcoI 809 CCATGG C^CATGG PinAI 1019 ACCGGT A^CCGGT PleI 1285 GACTC GAGTC(4/5) PpuMI 997 GGGACCC [GA]G^G[AT]CC[CT] PstI 1351 CTGCAG CTGCA^G SexAI 605 ACCTGGT A^CC[AT]GGT SfaNI 466 GATGC GCATC(5/9) SfcI 1351 CTGCAG C^T[GA][CT]AG SfoI 58 GGCGCC GGC^GCC SspBI 491 TGTACA T^GTACA TaqI 290 TCGA T^CGA TfiI 1540 GATTC G^A[AT]TC Tsp509I 362 AATT ^AATT TthHB8I 290 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 51 CGCG CG^CG 87 CGCG AlwNI 1102 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1310 CAGCCCCTG ApaI 500 GGGCCC GGGCC^C 689 GGGCCC AspHI 871 GTGCTC G[AT]GC[AT]^C 1275 GAGCAC AvaII 737 GGACC G^G[AT]CC 998 GGACC BanII 500 GGGCCC G[GA]GC[CT]^C 689 GGGCCC BbsI 820 GTCTTC GAAGAC(2/6) 895 GTCTTC BpuAI 820 GTCTTC GAAGAC(2/6) 895 GTCTTC BsaBI 489 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 675 GATACTGATC BsaWI 734 TCCGGA [AT]^CCGG[AT] 1019 ACCGGT BsiHKAI 871 GTGCTC G[AT]GC[AT]^C 1275 GAGCAC BsmAI 1399 GAGAC GTCTC(1/5) 931 GTCTC BsrFI 1019 ACCGGT [GA]^CCGG[CT] 1305 ACCGGC BstUI 51 CGCG CG^CG 87 CGCG Cfr10I 1019 ACCGGT [GA]^CCGG[CT] 1305 ACCGGC DraII 689 GGGCCCT [GA]G^G[ACGT]CC[CT] 997 GGGACCC DsaI 635 CCACGG C^C[GA][CT]GG 809 CCATGG EcoO109I 689 GGGCCCT [GA]G^G[ACGT]CC[CT] 997 GGGACCC FauI 70 GCGGG CCCGC(4/6) 777 GCGGG HaeII 58 GGCGCC [GA]GCGC^[CT] 729 AGCGCT HinfI 1285 GACTC G^A[ACGT]TC 1540 GATTC MaeII 565 ACGT A^CGT 614 ACGT MamI 489 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 675 GATACTGATC MseI 239 TTAA T^TAA 1142 TTAA MvnI 51 CGCG CG^CG 87 CGCG PflMI 504 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1101 CCAGCTCCTGG PvuII 112 CAGCTG CAG^CTG 1190 CAGCTG SapI 1450 GAAGAGC GCTCTTC(1/4) 342 GCTCTTC Tru9I 239 TTAA T^TAA 1142 TTAA Tsp45I 223 GTCAC ^GT[GC]AC 388 GTGAC Van91I 504 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1101 CCAGCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI BanI BbvCI BbvI BmyI BpmI BsaJI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TseI TspRI XhoII