(Circular) (Six-base) MAPSORT of: pFN21AE9433.seq Check: 9390 from: 1 to: 6250 pFN21AE9433 6250 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2585 3236 2585 Size: 651 5599 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4060 278 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2865 3593 1937 Size: 928 728 4594 Fragments arranged by size: 4594 928 728 Acc65I G'GTAC_C Cuts at: 3571 3571 Size: 6250 AclI AA'CG_TT Cuts at: 1563 4377 4750 1563 Size: 2814 373 3063 Fragments arranged by size: 3063 2814 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4313 5697 5965 1632 Size: 2681 1384 268 1917 Fragments arranged by size: 2681 1917 1384 268 AfeI AGC'GCT Cuts at: 2736 2736 Size: 6250 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6053 AflIII A'CryG_T Cuts at: 1203 2055 5149 6126 1203 Size: 852 3094 977 1327 Fragments arranged by size: 3094 1327 977 852 AgeI A'CCGG_T Cuts at: 3024 5081 3024 Size: 2057 4193 AhdI GACnn_n'nnGTC Cuts at: 4979 4979 Size: 6250 AleI CACnn'nnGTG Cuts at: 1166 2141 2213 1166 Size: 975 72 5203 Fragments arranged by size: 5203 975 72 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6250 AlwNI CAG_nnn'CTG Cuts at: 3112 3320 3993 5565 3112 Size: 208 673 1572 3797 Fragments arranged by size: 3797 1572 673 208 ApaI G_GGCC'C Cuts at: 2509 2698 2509 Size: 189 6061 ApaLI G'TGCA_C Cuts at: 4307 5463 4307 Size: 1156 5094 ApoI r'AATT_y Cuts at: 1493 2366 3559 3785 3937 6046 1493 Size: 873 1193 226 152 2109 1697 Fragments arranged by size: 2109 1697 1193 873 226 152 AseI AT'TA_AT Cuts at: 160 1032 4804 160 Size: 872 3772 1606 Fragments arranged by size: 3772 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2260 3097 3575 1870 Size: 73 153 164 837 478 4545 Fragments arranged by size: 4545 837 478 164 153 73 BamHI G'GATC_C Cuts at: 3580 3947 3580 Size: 367 5883 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 3307 3475 3571 5026 Size: 325 217 627 276 1244 168 96 1455 Cuts at: 5026 618 Size: 1842 Fragments arranged by size: 1842 1455 1244 627 325 276 217 168 96 BanII G_rGCy'C Cuts at: 1391 2509 2698 3569 1391 Size: 1118 189 871 4072 Fragments arranged by size: 4072 1118 871 189 BbeI G_GCGC'C Cuts at: 2067 2067 Size: 6250 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2818 2893 928 Size: 971 919 75 4285 Fragments arranged by size: 4285 971 919 75 BbvCI CC'TCA_GC Cuts at: 2306 2402 3069 2306 Size: 96 667 5487 Fragments arranged by size: 5487 667 96 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2077 2111 4441 4475 1903 Size: 34 140 34 2330 34 3678 Fragments arranged by size: 3678 2330 140 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3307 4142 5352 3307 Size: 835 1210 4205 Fragments arranged by size: 4205 1210 835 BclI T'GATC_A Cuts at: 1548 1548 Size: 6250 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4861 136 Size: 107 122 71 631 605 3189 1525 Fragments arranged by size: 3189 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3141 6245 3141 Size: 3104 3146 BlpI GC'TnA_GC Cuts at: 3193 3667 3193 Size: 474 5776 Bme1580I G_kGCm'C Cuts at: 1790 2292 2509 2698 2847 3480 4311 5467 1790 Size: 502 217 189 149 633 831 1156 2573 Fragments arranged by size: 2573 1156 831 633 502 217 189 149 BmgBI CAC'GTC Cuts at: 1318 2571 1318 Size: 1253 4997 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3981 4939 442 Size: 416 53 83 399 24 2564 958 1753 Fragments arranged by size: 2564 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 2611 3134 3254 4910 1239 Size: 1235 137 523 120 1656 2579 Fragments arranged by size: 2579 1656 1235 523 137 120 Bpu10I CC'TnA_GC Cuts at: 2306 2402 3069 3278 2306 Size: 96 667 209 5278 Fragments arranged by size: 5278 667 209 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4375 5240 5538 4375 Size: 865 298 5087 Fragments arranged by size: 5087 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3398 4913 882 Size: 617 1899 1515 2219 Fragments arranged by size: 2219 1899 1515 617 BsaAI yAC'GTr Cuts at: 493 1204 4041 493 Size: 711 2837 2702 Fragments arranged by size: 2837 2702 711 BsaBI GATnn'nnATC Cuts at: 2498 2684 6244 2498 Size: 186 3560 2504 Fragments arranged by size: 3560 2504 186 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 4057 4439 5074 Size: 53 83 186 936 531 1993 382 635 Cuts at: 5074 275 Size: 1451 Fragments arranged by size: 1993 1451 936 635 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2739 3024 4683 5081 5355 5502 5913 2739 Size: 285 1659 398 274 147 411 3076 Fragments arranged by size: 3076 1659 411 398 285 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6025 Fragments arranged by size: 6025 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3378 1186 Size: 2192 4058 BseYI C'CCAG_C Cuts at: 2778 3086 5453 5949 2778 Size: 308 2367 496 3079 Fragments arranged by size: 3079 2367 496 308 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 2563 2780 1163 Size: 573 429 398 217 4633 Fragments arranged by size: 4633 573 429 398 217 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3700 4461 5489 1540 Size: 275 186 1699 761 1028 2301 Fragments arranged by size: 2301 1699 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2880 3284 4311 4396 5467 2880 Size: 404 1027 85 1071 3663 Fragments arranged by size: 3663 1071 1027 404 85 BsmI GAATG_Cn' Cuts at: 3761 3854 3761 Size: 93 6157 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2292 2509 2698 2847 2880 3284 3480 Size: 399 502 217 189 149 33 404 196 Cuts at: 3480 3569 4311 4396 5467 1391 Size: 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 502 404 399 217 196 189 149 89 85 33 BspEI T'CCGG_A Cuts at: 2739 5913 2739 Size: 3174 3076 BspHI T'CATG_A Cuts at: 4139 5869 4139 Size: 1730 4520 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2137 2585 3236 3605 844 Size: 1293 448 651 369 3489 Fragments arranged by size: 3489 1293 651 448 369 BsrBI CCG'CTC Cuts at: 1394 1823 3499 4137 1394 Size: 429 1676 638 3507 Fragments arranged by size: 3507 1676 638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4745 4919 65 Size: 1183 3497 174 1396 Fragments arranged by size: 3497 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3024 3310 4894 5081 1700 Size: 110 1214 286 1584 187 2869 Fragments arranged by size: 2869 1584 1214 286 187 110 BsrGI T'GTAC_A Cuts at: 96 2496 96 Size: 2400 3850 BssHII G'CGCG_C Cuts at: 1925 2091 1925 Size: 166 6084 BssSI C'ACGA_G Cuts at: 4310 5322 4310 Size: 1012 5238 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6250 BstBI TT'CG_AA Cuts at: 5054 5054 Size: 6250 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6250 BstXI CCAn_nnnn'nTGG Cuts at: 2821 2821 Size: 6250 BstYI r'GATC_y Cuts at: 1972 2025 2334 3141 3580 3947 4332 4349 5801 Size: 53 309 807 439 367 385 17 1452 Cuts at: 5801 5887 5899 6245 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 807 439 385 367 346 309 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2640 2814 513 Size: 552 939 636 174 3949 Fragments arranged by size: 3949 939 636 552 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5511 Fragments arranged by size: 5511 683 56 BtsI GCAGTG_nn' Cuts at: 2520 2913 3766 4560 4580 2520 Size: 393 853 794 20 4190 Fragments arranged by size: 4190 853 794 393 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2629 2664 529 Size: 35 2065 35 4115 Fragments arranged by size: 4115 2065 35 35 DraI TTT'AAA Cuts at: 3554 3554 Size: 6250 DraIII CAC_nnn'GTG Cuts at: 2614 2614 Size: 6250 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2897 5257 809 Size: 507 1581 2360 1802 Fragments arranged by size: 2360 1802 1581 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2520 3697 4586 Size: 54 1425 50 275 18 690 1177 889 Cuts at: 4586 6215 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1177 889 690 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3697 1537 Size: 275 1885 4090 Fragments arranged by size: 4090 1885 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2354 2678 3162 3450 4180 1353 Size: 1001 324 484 288 730 3423 Fragments arranged by size: 3423 1001 730 484 324 288 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4837 5211 5357 387 Size: 1064 138 3248 374 146 1280 Fragments arranged by size: 3248 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2474 2611 3134 3254 4313 4910 5697 Size: 393 842 137 523 120 1059 597 787 Cuts at: 5697 5965 1239 Size: 268 1524 Fragments arranged by size: 1524 1059 842 787 597 523 393 268 137 120 EcoO109I rG'GnC_Cy Cuts at: 2695 3003 2695 Size: 308 5942 EcoRI G'AATT_C Cuts at: 3559 3937 3559 Size: 378 5872 FspI TGC'GCA Cuts at: 4756 4756 Size: 6250 HaeII r_GCGC'y Cuts at: 1678 2067 2738 5397 1678 Size: 389 671 2659 2531 Fragments arranged by size: 2659 2531 671 389 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 3281 3313 4891 Size: 32 135 32 890 32 787 32 1578 Cuts at: 4891 4923 4965 4997 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1578 890 787 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2199 3594 3840 669 Size: 988 281 261 1395 246 3079 Fragments arranged by size: 3079 1395 988 281 261 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6250 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KasI G'GCGC_C Cuts at: 2063 2063 Size: 6250 KpnI G_GTAC'C Cuts at: 3575 3575 Size: 6250 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 2055 6126 2055 Size: 4071 2179 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2713 5364 5548 1305 Size: 1408 2651 184 2007 Fragments arranged by size: 2651 2007 1408 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2522 10 Size: 54 1425 343 690 3738 Fragments arranged by size: 3738 1425 690 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2213 2819 4208 4567 4726 518 Size: 648 975 72 606 1389 359 159 2042 Fragments arranged by size: 2042 1389 975 648 606 359 159 72 MspA1I CmG'CkG Cuts at: 1606 2119 2782 3197 3666 4343 5491 5736 1606 Size: 513 663 415 469 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 663 513 469 415 245 NarI GG'CG_CC Cuts at: 2064 2064 Size: 6250 NcoI C'CATG_G Cuts at: 513 1065 2004 2814 513 Size: 552 939 810 3949 Fragments arranged by size: 3949 939 810 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6250 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6250 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NspI r_CATG'y Cuts at: 1132 1229 3608 5153 1132 Size: 97 2379 1545 2229 Fragments arranged by size: 2379 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6250 PciI A'CATG_T Cuts at: 5149 5149 Size: 6250 PflMI CCAn_nnn'nTGG Cuts at: 1108 2515 3112 1108 Size: 1407 597 4246 Fragments arranged by size: 4246 1407 597 PfoI T'CCnGG_A Cuts at: 3111 3111 Size: 6250 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2744 2776 4319 4351 5832 5864 2744 Size: 32 1543 32 1481 32 3130 Fragments arranged by size: 3130 1543 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3003 3003 Size: 6250 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 3820 3820 Size: 6250 PspOMI G'GGCC_C Cuts at: 2505 2694 2505 Size: 189 6061 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2114 2146 3075 3107 2114 Size: 32 929 32 5257 Fragments arranged by size: 5257 929 32 32 PstI C_TGCA'G Cuts at: 830 3360 3602 830 Size: 2530 242 3478 Fragments arranged by size: 3478 2530 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 PvuII CAG'CTG Cuts at: 2119 3197 2119 Size: 1078 5172 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SanDI GG'GwC_CC Cuts at: 3003 3003 Size: 6250 SapI GCTCTTCn'nnn_ Cuts at: 2354 3450 2354 Size: 1096 5154 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 4498 6210 4498 Size: 1712 4538 SexAI A'CCwGG_T Cuts at: 2610 2610 Size: 6250 SfcI C'TryA_G Cuts at: 826 1045 3356 3598 4733 5414 5605 826 Size: 219 2311 242 1135 681 191 1471 Fragments arranged by size: 2311 1471 1135 681 242 219 191 SfoI GGC'GCC Cuts at: 2065 2065 Size: 6250 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 3577 1872 Size: 1705 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4354 5255 5517 820 Size: 197 926 2411 901 262 1553 Fragments arranged by size: 2411 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6250 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SspI AAT'ATT Cuts at: 5 52 4174 5 Size: 47 4122 2081 Fragments arranged by size: 4122 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6250 StyI C'CwwG_G Cuts at: 513 1065 2004 2601 2814 3210 3438 3480 3689 Size: 552 939 597 213 396 228 42 209 Cuts at: 3689 513 Size: 3074 Fragments arranged by size: 3074 939 597 552 396 228 213 209 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4277 4294 4447 4632 5077 325 Size: 1255 2697 17 153 185 445 1498 Fragments arranged by size: 2697 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 3050 3183 Size: 274 80 33 51 1443 519 554 133 Cuts at: 3183 4496 6208 96 Size: 1313 1712 138 Fragments arranged by size: 1712 1443 1313 554 519 274 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 6210 6242 2042 Size: 32 4136 32 2050 Fragments arranged by size: 4136 2050 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6250 XbaI T'CTAG_A Cuts at: 3586 3586 Size: 6250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 3575 1870 Size: 1705 4545 XmnI GAAnn'nnTTC Cuts at: 4379 4379 Size: 6250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4058 276 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SfiI SrfI SwaI XcmI