EHD3 ORF clone FHC10763

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10763

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Clone info.

Species

human

Clone Name

pFN21AE0094 Sequence (with vector) Restriction Map

Product ID

FHC10763

Flexi Clone PID

FXC10763

ORF Sequence

DNA sequence (1605 bp)
Protein sequence (535 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527358]

Gene

KIEE0094

Symbol

EHD3

Alias : PAST3

Description

EH-domain containing 3

Original Clone ID

oc00794

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1605 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 535 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1774.1 8.9e-209 100.0 EHD3 CCDS8084.1 2e-186 87.0 EHD1 CCDS73315.1 2e-186 87.0 EHD1 CCDS10081.1 7.6e-160 75.3 EHD4 CCDS12704.1 2.2e-151 71.7 EHD2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001401 61 221 PF00350 Dynamin HMMSmart IPR000261 438 531 SM00027 EPS15 homology (EH) ProfileScan IPR000261 444 532 PS50031 EPS15 homology (EH) IPR018249 476 511 PS50222 EF-HAND 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055415 535 605891 100.0 100.0 XP_011543041 534 605888 87.0 99.4 NP_006786 534 605888 87.0 99.4 NP_001269373 534 605888 87.0 99.4 NP_001269374 548 605888 87.0 99.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0094 Download

>KIEE0094  1605 bp
ATGTTCAGCTGGCTGGGTACGGACGACCGCCGGAGGAAGGACCCCGAGGTTTTCCAGACG
GTGAGTGAGGGGCTCAAGAAACTCTACAAGAGCAAGCTGCTGCCCTTGGAAGAGCATTAC
CGCTTCCACGAGTTCCACTCGCCCGCCCTGGAGGATGCCGACTTCGACAACAAGCCCATG
GTTCTGCTGGTGGGCCAGTACTCCACTGGGAAGACCACCTTCATCAGGTACCTGCTGGAA
CAGGACTTCCCAGGCATGAGGATTGGGCCTGAGCCCACCACAGACTCCTTCATTGCGGTG
ATGCAGGGAGACATGGAGGGGATCATCCCTGGGAACGCCCTGGTGGTGGATCCCAAGAAA
CCCTTCAGGAAACTCAACGCCTTTGGCAACGCCTTCTTGAACAGGTTCGTGTGTGCCCAG
CTACCTAACCCTGTGCTGGAGAGCATCAGCGTCATCGACACACCAGGGATCCTCTCTGGG
GAGAAGCAGAGGATCAGCCGGGGGTATGACTTTGCAGCTGTCCTTGAGTGGTTTGCCGAG
CGGGTTGACCGCATCATTCTGCTCTTCGATGCCCACAAACTGGACATCTCTGATGAGTTC
TCAGAAGTCATCAAAGCCCTCAAGAACCACGAGGACAAGATGCGAGTGGTGCTGAACAAA
GCTGACCAGATCGAGACGCAGCAGCTGATGCGGGTGTACGGGGCCCTCATGTGGTCCTTG
GGGAAGATCGTGAACACCCCAGAGGTGATCCGGGTCTACATCGGCTCCTTCTGGTCCCAC
CCCCTCCTCATCCCTGACAACCGGAAGCTCTTTGAGGCTGAGGAACAGGACCTATTCAGG
GACATCCAGAGTCTGCCCCGAAATGCTGCCCTGCGCAAGCTCAACGACCTCATCAAAAGG
GCCAGGCTGGCCAAGGTCCACGCCTACATCATCAGCTCTCTGAAGAAGGAGATGCCCTCG
GTGTTCGGGAAGGACAACAAGAAGAAGGAGCTGGTCAACAACCTGGCCGAGATCTATGGC
CGGATCGAGCGGGAGCACCAGATCTCACCTGGGGACTTCCCCAATCTGAAGAGGATGCAG
GACCAGCTGCAGGCCCAGGACTTTAGCAAGTTCCAGCCGCTGAAGAGCAAGCTGCTGGAG
GTAGTGGACGACATGCTGGCCCATGACATTGCCCAGCTCATGGTGCTAGTGCGCCAGGAG
GAGTCACAGCGGCCCATCCAGATGGTGAAGGGCGGAGCGTTCGAGGGCACCCTGCACGGC
CCCTTTGGGCATGGCTATGGGGAGGGGGCTGGAGAAGGTATCGATGATGCTGAGTGGGTG
GTGGCCAGGGACAAGCCCATGTACGACGAGATCTTCTACACCCTGTCACCGGTGGATGGC
AAGATCACAGGCGCTAATGCCAAGAAGGAGATGGTGCGCTCCAAGCTGCCCAACAGTGTG
CTGGGCAAGATCTGGAAGCTGGCCGACATTGACAAGGATGGCATGCTGGACGACGACGAG
TTTGCACTGGCCAACCACCTCATCAAAGTCAAGCTGGAGGGGCACGAGCTGCCCAACGAG
CTGCCTGCCCACCTCCTGCCCCCGTCCAAGAGGAAAGTTGCCGAG

ORF Protein

Cloned ORF protein sequence for pFN21AE0094 Download

>KIEE0094  535 aa
MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPM
VLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKK
PFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAE
RVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSL
GKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKR
ARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQ
DQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHG
PFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSV
LGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0094 Download

>pFN21AE0094  6310 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTCAGCTGGCTGGGTACGGACGACCGCCGGAG
GAAGGACCCCGAGGTTTTCCAGACGGTGAGTGAGGGGCTCAAGAAACTCTACAAGAGCAA
GCTGCTGCCCTTGGAAGAGCATTACCGCTTCCACGAGTTCCACTCGCCCGCCCTGGAGGA
TGCCGACTTCGACAACAAGCCCATGGTTCTGCTGGTGGGCCAGTACTCCACTGGGAAGAC
CACCTTCATCAGGTACCTGCTGGAACAGGACTTCCCAGGCATGAGGATTGGGCCTGAGCC
CACCACAGACTCCTTCATTGCGGTGATGCAGGGAGACATGGAGGGGATCATCCCTGGGAA
CGCCCTGGTGGTGGATCCCAAGAAACCCTTCAGGAAACTCAACGCCTTTGGCAACGCCTT
CTTGAACAGGTTCGTGTGTGCCCAGCTACCTAACCCTGTGCTGGAGAGCATCAGCGTCAT
CGACACACCAGGGATCCTCTCTGGGGAGAAGCAGAGGATCAGCCGGGGGTATGACTTTGC
AGCTGTCCTTGAGTGGTTTGCCGAGCGGGTTGACCGCATCATTCTGCTCTTCGATGCCCA
CAAACTGGACATCTCTGATGAGTTCTCAGAAGTCATCAAAGCCCTCAAGAACCACGAGGA
CAAGATGCGAGTGGTGCTGAACAAAGCTGACCAGATCGAGACGCAGCAGCTGATGCGGGT
GTACGGGGCCCTCATGTGGTCCTTGGGGAAGATCGTGAACACCCCAGAGGTGATCCGGGT
CTACATCGGCTCCTTCTGGTCCCACCCCCTCCTCATCCCTGACAACCGGAAGCTCTTTGA
GGCTGAGGAACAGGACCTATTCAGGGACATCCAGAGTCTGCCCCGAAATGCTGCCCTGCG
CAAGCTCAACGACCTCATCAAAAGGGCCAGGCTGGCCAAGGTCCACGCCTACATCATCAG
CTCTCTGAAGAAGGAGATGCCCTCGGTGTTCGGGAAGGACAACAAGAAGAAGGAGCTGGT
CAACAACCTGGCCGAGATCTATGGCCGGATCGAGCGGGAGCACCAGATCTCACCTGGGGA
CTTCCCCAATCTGAAGAGGATGCAGGACCAGCTGCAGGCCCAGGACTTTAGCAAGTTCCA
GCCGCTGAAGAGCAAGCTGCTGGAGGTAGTGGACGACATGCTGGCCCATGACATTGCCCA
GCTCATGGTGCTAGTGCGCCAGGAGGAGTCACAGCGGCCCATCCAGATGGTGAAGGGCGG
AGCGTTCGAGGGCACCCTGCACGGCCCCTTTGGGCATGGCTATGGGGAGGGGGCTGGAGA
AGGTATCGATGATGCTGAGTGGGTGGTGGCCAGGGACAAGCCCATGTACGACGAGATCTT
CTACACCCTGTCACCGGTGGATGGCAAGATCACAGGCGCTAATGCCAAGAAGGAGATGGT
GCGCTCCAAGCTGCCCAACAGTGTGCTGGGCAAGATCTGGAAGCTGGCCGACATTGACAA
GGATGGCATGCTGGACGACGACGAGTTTGCACTGGCCAACCACCTCATCAAAGTCAAGCT
GGAGGGGCACGAGCTGCCCAACGAGCTGCCTGCCCACCTCCTGCCCCCGTCCAAGAGGAA
AGTTGCCGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT