(Circular) (Six-base) MAPSORT of: pFN21AE0094.seq Check: 4607 from: 1 to: 6310 pFN21AE0094 6310 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4120 278 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 3653 1937 Size: 823 893 4594 Fragments arranged by size: 4594 893 823 Acc65I G'GTAC_C Cuts at: 2232 3631 2232 Size: 1399 4911 AclI AA'CG_TT Cuts at: 1563 4437 4810 1563 Size: 2874 373 3063 Fragments arranged by size: 3063 2874 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2353 2966 3092 3146 4373 5757 6025 1632 Size: 721 613 126 54 1227 1384 268 1917 Fragments arranged by size: 1917 1384 1227 721 613 268 126 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6113 AflIII A'CryG_T Cuts at: 1203 5209 6186 1203 Size: 4006 977 1327 Fragments arranged by size: 4006 1327 977 AgeI A'CCGG_T Cuts at: 3373 5141 3373 Size: 1768 4542 AhdI GACnn_n'nnGTC Cuts at: 5039 5039 Size: 6310 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2120 2579 1184 Size: 936 459 4915 Fragments arranged by size: 4915 936 459 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2235 2267 2235 Size: 32 6278 AlwNI CAG_nnn'CTG Cuts at: 2689 2857 3124 4053 5625 2689 Size: 168 267 929 1572 3374 Fragments arranged by size: 3374 1572 929 267 168 ApaI G_GGCC'C Cuts at: 2710 2710 Size: 6310 ApaLI G'TGCA_C Cuts at: 4367 5523 4367 Size: 1156 5154 ApoI r'AATT_y Cuts at: 1493 3619 3845 3997 6106 1493 Size: 2126 226 152 2109 1697 Fragments arranged by size: 2126 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4864 160 Size: 872 3832 1606 Fragments arranged by size: 3832 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 AvaI C'yCGr_G Cuts at: 1870 1943 2048 3635 1870 Size: 73 105 1587 4545 Fragments arranged by size: 4545 1587 105 73 BamHI G'GATC_C Cuts at: 2353 2472 3640 4007 2353 Size: 119 1168 367 4656 Fragments arranged by size: 4656 1168 367 119 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 3251 3631 5086 618 Size: 325 217 627 445 1019 380 1455 1842 Fragments arranged by size: 1842 1455 1019 627 445 380 325 217 BanII G_rGCy'C Cuts at: 1391 2079 2280 2710 3629 1391 Size: 688 201 430 919 4072 Fragments arranged by size: 4072 919 688 430 201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2222 928 Size: 971 323 5016 Fragments arranged by size: 5016 971 323 BbvCI CC'TCA_GC Cuts at: 2823 2823 Size: 6310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3488 3522 3551 3585 4501 4535 1903 Size: 34 1551 34 29 34 916 34 3678 Fragments arranged by size: 3678 1551 916 34 34 34 34 29 BciVI GTATCCnnnnn_n' Cuts at: 4202 5412 4202 Size: 1210 5100 BclI T'GATC_A Cuts at: 1548 1548 Size: 6310 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2912 4921 136 Size: 107 122 71 631 605 1240 2009 1525 Fragments arranged by size: 2009 1525 1240 631 605 122 107 71 BglII A'GATC_T Cuts at: 3015 3045 3354 3453 6305 3015 Size: 30 309 99 2852 3020 Fragments arranged by size: 3020 2852 309 99 30 BlpI GC'TnA_GC Cuts at: 3727 3727 Size: 6310 Bme1580I G_kGCm'C Cuts at: 1790 2422 2710 3254 3549 4371 5527 1790 Size: 632 288 544 295 822 1156 2573 Fragments arranged by size: 2573 1156 822 632 544 295 288 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6310 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2220 4041 4999 Size: 416 53 83 399 24 803 1821 958 Cuts at: 4999 442 Size: 1753 Fragments arranged by size: 1821 1753 958 803 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6310 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2158 2977 2158 Size: 819 5491 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2462 3161 3315 3560 4970 1239 Size: 935 288 699 154 245 1410 2579 Fragments arranged by size: 2579 1410 935 699 288 245 154 Bpu10I CC'TnA_GC Cuts at: 2274 2823 2274 Size: 549 5761 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 2549 2609 4435 5300 5598 2063 Size: 486 60 1826 865 298 2775 Fragments arranged by size: 2775 1826 865 486 298 60 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4973 882 Size: 617 3474 2219 Fragments arranged by size: 3474 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4101 493 Size: 711 2897 2702 Fragments arranged by size: 2897 2702 711 BsaBI GATnn'nnATC Cuts at: 6304 6304 Size: 6310 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4117 4499 5134 275 Size: 53 83 186 936 2584 382 635 1451 Fragments arranged by size: 2584 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2805 3373 4743 5141 5415 5562 5973 2805 Size: 568 1370 398 274 147 411 3142 Fragments arranged by size: 3142 1370 568 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 1178 Size: 30 165 30 1350 30 4705 Fragments arranged by size: 4705 1350 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2780 3218 1186 Size: 1594 438 4278 Fragments arranged by size: 4278 1594 438 BseYI C'CCAG_C Cuts at: 2017 2421 3177 3445 5513 6009 2017 Size: 404 756 268 2068 496 2318 Fragments arranged by size: 2318 2068 756 496 404 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3242 1163 Size: 573 1506 4231 Fragments arranged by size: 4231 1506 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2032 3760 4521 5549 1540 Size: 275 186 31 1728 761 1028 2301 Fragments arranged by size: 2301 1728 1028 761 275 186 31 BsiHKAI G_wGCw'C Cuts at: 3042 4371 4456 5527 3042 Size: 1329 85 1071 3825 Fragments arranged by size: 3825 1329 1071 85 BsmI GAATG_Cn' Cuts at: 3821 3914 3821 Size: 93 6217 BsmBI CGTCTCn'nnnn_ Cuts at: 2672 2672 Size: 6310 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2280 2422 2710 3042 3254 3549 Size: 399 289 201 142 288 332 212 295 Cuts at: 3549 3629 4371 4456 5527 1391 Size: 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 332 295 289 288 212 201 142 85 80 BspEI T'CCGG_A Cuts at: 5973 5973 Size: 6310 BspHI T'CATG_A Cuts at: 4199 5929 4199 Size: 1730 4580 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 3665 844 Size: 1400 1421 3489 Fragments arranged by size: 3489 1421 1400 BsrBI CCG'CTC Cuts at: 1394 1823 2545 3034 4197 1394 Size: 429 722 489 1163 3507 Fragments arranged by size: 3507 1163 722 489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2295 3171 4805 4979 65 Size: 1183 1047 876 1634 174 1396 Fragments arranged by size: 1634 1396 1183 1047 876 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3373 4954 5141 1700 Size: 110 1563 1581 187 2869 Fragments arranged by size: 2869 1581 1563 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6310 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6310 BssSI C'ACGA_G Cuts at: 2132 2633 3548 4370 5382 2132 Size: 501 915 822 1012 3060 Fragments arranged by size: 3060 1012 915 822 501 BstAPI GCAn_nnn'nTGC Cuts at: 794 2692 3553 794 Size: 1898 861 3551 Fragments arranged by size: 3551 1898 861 BstBI TT'CG_AA Cuts at: 5114 5114 Size: 6310 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6310 BstXI CCAn_nnnn'nTGG Cuts at: 3226 3226 Size: 6310 BstYI r'GATC_y Cuts at: 1972 2353 2472 3015 3045 3354 3453 3640 4007 Size: 381 119 543 30 309 99 187 367 Cuts at: 4007 4392 4409 5861 5947 5959 6305 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 543 385 381 367 346 309 187 119 99 86 30 17 12 BtgI C'CryG_G Cuts at: 513 1065 2181 513 Size: 552 1116 4642 Fragments arranged by size: 4642 1116 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5571 Fragments arranged by size: 5571 683 56 BtsI GCAGTG_nn' Cuts at: 3826 4620 4640 3826 Size: 794 20 5496 Fragments arranged by size: 5496 794 20 ClaI AT'CG_AT Cuts at: 1554 3306 1554 Size: 1752 4558 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6275 DraI TTT'AAA Cuts at: 3614 3614 Size: 6310 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5317 809 Size: 507 4001 1802 Fragments arranged by size: 4001 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2913 3009 3022 Size: 54 1425 50 275 18 1083 96 13 Cuts at: 3022 3327 3465 3513 3757 4646 6275 8 Size: 305 138 48 244 889 1629 43 Fragments arranged by size: 1629 1425 1083 889 305 275 244 138 96 54 50 48 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3757 1537 Size: 275 1945 4090 Fragments arranged by size: 4090 1945 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2573 3068 3122 4240 1353 Size: 756 464 495 54 1118 3423 Fragments arranged by size: 3423 1118 756 495 464 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3252 4897 5271 5417 387 Size: 1064 138 1663 1645 374 146 1280 Fragments arranged by size: 1663 1645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2353 2462 2966 3092 3146 3161 Size: 393 542 179 109 504 126 54 15 Cuts at: 3161 3315 3560 4373 4970 5757 6025 1239 Size: 154 245 813 597 787 268 1524 Fragments arranged by size: 1524 813 787 597 542 504 393 268 245 179 154 126 109 54 15 EcoNI CCTnn'n_nnAGG Cuts at: 2227 2227 Size: 6310 EcoO109I rG'GnC_Cy Cuts at: 2044 2706 2707 2833 2044 Size: 662 1 126 5521 Fragments arranged by size: 5521 662 126 1 EcoRI G'AATT_C Cuts at: 3619 3997 3619 Size: 378 5932 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 2383 2239 Size: 144 6166 FspI TGC'GCA Cuts at: 2879 4816 2879 Size: 1937 4373 HaeII r_GCGC'y Cuts at: 1678 3399 5457 1678 Size: 1721 2058 2531 Fragments arranged by size: 2531 2058 1721 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 3288 3320 4951 Size: 32 135 32 1016 32 668 32 1631 Cuts at: 4951 4983 5025 5057 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1631 1016 668 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2551 3001 3654 3900 669 Size: 988 281 613 450 653 246 3079 Fragments arranged by size: 3079 988 653 613 450 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6310 HpaI GTT'AAC Cuts at: 3900 3900 Size: 6310 KpnI G_GTAC'C Cuts at: 2236 3635 2236 Size: 1399 4911 MfeI C'AATT_G Cuts at: 3909 3909 Size: 6310 MluI A'CGCG_T Cuts at: 6186 6186 Size: 6310 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5424 5608 1305 Size: 4119 184 2007 Fragments arranged by size: 4119 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2915 3329 3515 10 Size: 54 1425 343 1083 414 186 2805 Fragments arranged by size: 2805 1425 1083 414 343 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 3224 3279 4268 4627 4786 518 Size: 648 1134 924 55 989 359 159 2042 Fragments arranged by size: 2042 1134 989 924 648 359 159 55 MspA1I CmG'CkG Cuts at: 1606 2013 2522 2689 3091 3124 3214 3726 4403 Size: 407 509 167 402 33 90 512 677 Cuts at: 4403 5551 5796 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 512 509 407 402 245 167 90 33 NcoI C'CATG_G Cuts at: 513 1065 2181 513 Size: 552 1116 4642 Fragments arranged by size: 4642 1116 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6310 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6310 NotI GC'GGCC_GC Cuts at: 3757 3757 Size: 6310 NspI r_CATG'y Cuts at: 1132 1229 3160 3490 3668 5213 1132 Size: 97 1931 330 178 1545 2229 Fragments arranged by size: 2229 1931 1545 330 178 97 PasI CC'CwG_GG Cuts at: 1348 2333 1348 Size: 985 5325 PciI A'CATG_T Cuts at: 5209 5209 Size: 6310 PflMI CCAn_nnn'nTGG Cuts at: 1108 2584 3184 1108 Size: 1476 600 4234 Fragments arranged by size: 4234 1476 600 PmeI GTTT'AAAC Cuts at: 3614 3614 Size: 6310 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4379 4411 5892 5924 4379 Size: 32 1481 32 4765 Fragments arranged by size: 4765 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2044 2833 2044 Size: 789 5521 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6310 PsiI TTA'TAA Cuts at: 3880 3880 Size: 6310 PspOMI G'GGCC_C Cuts at: 2706 2706 Size: 6310 PstI C_TGCA'G Cuts at: 830 3096 3662 830 Size: 2266 566 3478 Fragments arranged by size: 3478 2266 566 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6310 PvuII CAG'CTG Cuts at: 2013 2522 2689 3091 2013 Size: 509 167 402 5232 Fragments arranged by size: 5232 509 402 167 SalI G'TCGA_C Cuts at: 1936 3652 1936 Size: 1716 4594 SapI GCTCTTCn'nnn_ Cuts at: 2109 2573 3122 2109 Size: 464 549 5297 Fragments arranged by size: 5297 549 464 SbfI CC_TGCA'GG Cuts at: 3662 3662 Size: 6310 ScaI AGT'ACT Cuts at: 2204 4558 6270 2204 Size: 2354 1712 2244 Fragments arranged by size: 2354 2244 1712 SfcI C'TryA_G Cuts at: 826 1045 3092 3658 4793 5474 5665 826 Size: 219 2047 566 1135 681 191 1471 Fragments arranged by size: 2047 1471 1135 681 566 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2912 2912 Size: 6310 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 SgrAI Cr'CCGG_yG Cuts at: 1700 3373 1700 Size: 1673 4637 SmaI CCC'GGG Cuts at: 1872 3637 1872 Size: 1765 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2528 2624 4414 5315 5577 Size: 197 926 135 450 96 1790 901 262 Cuts at: 5577 820 Size: 1553 Fragments arranged by size: 1790 1553 926 901 450 262 197 135 96 SnaBI TAC'GTA Cuts at: 493 4101 493 Size: 3608 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6310 SphI G_CATG'C Cuts at: 1132 3490 3668 1132 Size: 2358 178 3774 Fragments arranged by size: 3774 2358 178 SspI AAT'ATT Cuts at: 5 52 4234 5 Size: 47 4182 2081 Fragments arranged by size: 4182 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6310 StyI C'CwwG_G Cuts at: 513 1065 2109 2181 2721 2916 3749 513 Size: 552 1044 72 540 195 833 3074 Fragments arranged by size: 3074 1044 833 552 540 195 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3310 4337 4354 4507 4692 5137 325 Size: 1255 1730 1027 17 153 185 445 1498 Fragments arranged by size: 1730 1498 1255 1027 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 4556 6268 Size: 274 80 33 51 1443 225 2354 1712 Cuts at: 6268 96 Size: 138 Fragments arranged by size: 2354 1712 1443 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2457 2489 6270 6302 2457 Size: 32 3781 32 2465 Fragments arranged by size: 3781 2465 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6310 XbaI T'CTAG_A Cuts at: 3646 3646 Size: 6310 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2188 2207 3050 3412 3442 2188 Size: 19 843 362 30 5056 Fragments arranged by size: 5056 843 362 30 19 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6310 XmaI C'CCGG_G Cuts at: 1870 3635 1870 Size: 1765 4545 XmnI GAAnn'nnTTC Cuts at: 2366 2408 4439 2366 Size: 42 2031 4237 Fragments arranged by size: 4237 2031 42 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4118 276 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI