HCLS1 ORF clone FHC05400

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC05400

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Clone info.

Species

human

Clone Name

pFN21AB7742 Sequence (with vector) Restriction Map

Product ID

FHC05400

Flexi Clone PID

FXC05400

ORF Sequence

DNA sequence (1458 bp)
Protein sequence (486 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463725]

Gene

KIBB7742

Symbol

HCLS1

Alias : CTTNL, HS1, lckBP1, p75

Description

hematopoietic cell-specific Lyn substrate 1, transcript variant 1

Original Clone ID

cs00242

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1458 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 486 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3003.1 1.9e-91 99.6 HCLS1 CCDS77800.1 5.4e-65 92.0 HCLS1 CCDS8197.1 1.1e-27 44.3 CTTN CCDS53676.1 1.3e-27 43.6 CTTN CCDS41680.1 2.1e-27 41.0 CTTN The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000108 275 292 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 431 441 PR00452 Src homology-3 domain IPR000108 433 453 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 445 460 PR00452 Src homology-3 domain IPR000108 453 469 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 462 471 PR00452 Src homology-3 domain IPR000108 469 482 PR00499 Neutrophil cytosol factor 2 p67phox IPR001452 473 485 PR00452 Src homology-3 domain HMMPfam IPR003134 82 118 PF02218 Hs1/Cortactin IPR003134 119 155 PF02218 Hs1/Cortactin IPR003134 156 192 PF02218 Hs1/Cortactin IPR003134 193 213 PF02218 Hs1/Cortactin IPR001452 434 479 PF00018 Src homology-3 domain HMMSmart IPR001452 431 486 SM00326 Src homology-3 domain ProfileScan IPR003134 79 115 PS51090 Hs1/Cortactin IPR003134 116 152 PS51090 Hs1/Cortactin IPR003134 153 189 PS51090 Hs1/Cortactin IPR003134 190 212 PS51090 Hs1/Cortactin IPR001452 428 486 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005326 486 601306 99.8 100.0 NP_001278970 449 601306 92.2 100.0 XP_006718510 476 164765 45.6 99.6 NP_612632 513 164765 44.3 99.6 NP_001171669 634 164765 43.6 97.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7742 Download

>KIBB7742  1458 bp
ATGTGGAAGTCTGTAGTGGGCCATGATGTGTCTGTTTCCGTGGAGACCCAGGGTGATGAT
TGGGACACAGATCCTGACTTTGTGAATGACATCTCTGAAAAGGAGCAACGATGGGGAGCC
AAGACCATCGAGGGGTCTGGACGCACAGAACACATCAACATCCACCAGCTGAGGAACAAA
GTATCAGAGGAGCATGATGTTCTCAGGAAGAAAGAGATGGAGTCAGGGCCCAAAGCATCC
CATGGCTATGGAGGTCGGTTTGGAGTAGAAAGAGACCGAATGGACAAGAGTGCAGTGGGC
CATGAGTATGTTGCCGAGGTGGAGAAGCACTCTTCTCAGACGGATGCTGCCAAAGGCTTT
GGGGGCAAGTACGGAGTTGAGAGGGACAGGGCAGACAAGTCAGCAGTCGGCTTTGATTAT
AAAGGAGAAGTGGAGAAGCATACATCTCAGAAAGATTACTCTCGTGGCTTTGGTGGCCGG
TACGGGGTGGAGAAGGATAAATGGGACAAAGCAGCTCTGGGATATGACTACAAGGGAGAG
ACGGAGAAACACGAGTCCCAGAGAGATTATGCCAAGGGCTTTGGTGGCCAGTATGGAATC
CAGAAGGACCGAGTGGATAAGAGCGCTGTCGGCTTCAATGAAATGGAGGCCCCGACCACA
GCTTATAAGAAGACGACGCCCATAGAAGCCGCTTCTAGTGGTGCCCGTGGGCTGAAGGCG
AAATTTGAGTCCATGGCTGAGGAGAAGAGGAAGCGAGAGGAAGAGGAGAAGGCACAGCAG
GTGGCCAGGAGGCAACAGGAGCGAAAGGCTGTGACAAAGAGGAGCCCTGAGGCTCCACAG
CCAGTGATAGCTATGGAAGAGCCAGCAGTACCGGCCCCACTGCCCAAGAAAATCTCCTCA
GAGGCCTGGCCTCCAGTTGGGACTCCTCCATCATCAGAGTCTGAGCCTGTGAGAACCAGC
AGGGAACACCCAGTGCCCTTGCTGCCCATTAGGCAGACTCTCCCGGAGGACAATGAGGAG
CCCCCAGCTCTGCCCCCTAGGACTCTGGAAGGCCTCCAGGTGGAGGAAGAGCCAGTGTAC
GAAGCAGAGCCTGAGCCTGAGCCCGAGCCTGAGCCCGAGCCTGAGAATGACTATGAGGAC
GTTGAGGAGATGGACAGGCATGAGCAGGAGGATGAACCAGAGGGGGACTATGAGGAGGTG
CTCGAGCCTGAAGATTCTTCTTTTTCTTCTGCTCTGGCTGGATCATCAGGCTGCCCGGCT
GGGGCTGGGGCTGGGGCTGTGGCTCTGGGGATCTCAGCTGTGGCTCTATATGATTACCAA
GGAGAGGGAAGTGATGAGCTTTCCTTTGATCCGGACGACGTAATCACTGACATTGAGATG
GTGGACGAGGGCTGGTGGCGGGGACGTTGCCATGGCCACTTTGGACTCTTCCCTGCAAAT
TATGTCAAGCTTCTGGAG

ORF Protein

Cloned ORF protein sequence for pFN21AB7742 Download

>KIBB7742  486 aa
MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNK
VSEEHDVLRKKEMESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGF
GGKYGVERDRADKSAVGFDYKGEVEKHTSQKDYSRGFGGRYGVEKDKWDKAALGYDYKGE
TEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGARGLKA
KFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISS
EAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVEEEPVY
EAEPEPEPEPEPEPENDYEDVEEMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPA
GAGAGAVALGISAVALYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPAN
YVKLLE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7742 Download

>pFN21AB7742  6163 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGGAAGTCTGTAGTGGGCCATGATGTGTCTGT
TTCCGTGGAGACCCAGGGTGATGATTGGGACACAGATCCTGACTTTGTGAATGACATCTC
TGAAAAGGAGCAACGATGGGGAGCCAAGACCATCGAGGGGTCTGGACGCACAGAACACAT
CAACATCCACCAGCTGAGGAACAAAGTATCAGAGGAGCATGATGTTCTCAGGAAGAAAGA
GATGGAGTCAGGGCCCAAAGCATCCCATGGCTATGGAGGTCGGTTTGGAGTAGAAAGAGA
CCGAATGGACAAGAGTGCAGTGGGCCATGAGTATGTTGCCGAGGTGGAGAAGCACTCTTC
TCAGACGGATGCTGCCAAAGGCTTTGGGGGCAAGTACGGAGTTGAGAGGGACAGGGCAGA
CAAGTCAGCAGTCGGCTTTGATTATAAAGGAGAAGTGGAGAAGCATACATCTCAGAAAGA
TTACTCTCGTGGCTTTGGTGGCCGGTACGGGGTGGAGAAGGATAAATGGGACAAAGCAGC
TCTGGGATATGACTACAAGGGAGAGACGGAGAAACACGAGTCCCAGAGAGATTATGCCAA
GGGCTTTGGTGGCCAGTATGGAATCCAGAAGGACCGAGTGGATAAGAGCGCTGTCGGCTT
CAATGAAATGGAGGCCCCGACCACAGCTTATAAGAAGACGACGCCCATAGAAGCCGCTTC
TAGTGGTGCCCGTGGGCTGAAGGCGAAATTTGAGTCCATGGCTGAGGAGAAGAGGAAGCG
AGAGGAAGAGGAGAAGGCACAGCAGGTGGCCAGGAGGCAACAGGAGCGAAAGGCTGTGAC
AAAGAGGAGCCCTGAGGCTCCACAGCCAGTGATAGCTATGGAAGAGCCAGCAGTACCGGC
CCCACTGCCCAAGAAAATCTCCTCAGAGGCCTGGCCTCCAGTTGGGACTCCTCCATCATC
AGAGTCTGAGCCTGTGAGAACCAGCAGGGAACACCCAGTGCCCTTGCTGCCCATTAGGCA
GACTCTCCCGGAGGACAATGAGGAGCCCCCAGCTCTGCCCCCTAGGACTCTGGAAGGCCT
CCAGGTGGAGGAAGAGCCAGTGTACGAAGCAGAGCCTGAGCCTGAGCCCGAGCCTGAGCC
CGAGCCTGAGAATGACTATGAGGACGTTGAGGAGATGGACAGGCATGAGCAGGAGGATGA
ACCAGAGGGGGACTATGAGGAGGTGCTCGAGCCTGAAGATTCTTCTTTTTCTTCTGCTCT
GGCTGGATCATCAGGCTGCCCGGCTGGGGCTGGGGCTGGGGCTGTGGCTCTGGGGATCTC
AGCTGTGGCTCTATATGATTACCAAGGAGAGGGAAGTGATGAGCTTTCCTTTGATCCGGA
CGACGTAATCACTGACATTGAGATGGTGGACGAGGGCTGGTGGCGGGGACGTTGCCATGG
CCACTTTGGACTCTTCCCTGCAAATTATGTCAAGCTTCTGGAGGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT