(Circular) (Six-base) MAPSORT of: pFN21AB7742.seq Check: 48 from: 1 to: 6163 pFN21AB7742 6163 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2773 2773 Size: 6163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3506 1937 Size: 1569 4594 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2738 3234 4226 5610 5878 1632 Size: 1106 496 992 1384 268 1917 Fragments arranged by size: 1917 1384 1106 992 496 268 AfeI AGC'GCT Cuts at: 2629 2629 Size: 6163 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5966 AflIII A'CryG_T Cuts at: 1203 5062 6039 1203 Size: 3859 977 1327 Fragments arranged by size: 3859 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AlwNI CAG_nnn'CTG Cuts at: 2078 2945 3095 3906 5478 2078 Size: 867 150 811 1572 2763 Fragments arranged by size: 2763 1572 867 811 150 ApaI G_GGCC'C Cuts at: 2235 2235 Size: 6163 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 2726 3472 3698 3850 5959 1493 Size: 1233 746 226 152 2109 1697 Fragments arranged by size: 2109 1697 1233 746 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 3107 3119 3206 3488 1870 Size: 73 1164 12 87 282 4545 Fragments arranged by size: 4545 1164 282 87 73 12 AvrII C'CTAG_G Cuts at: 3041 3041 Size: 6163 BamHI G'GATC_C Cuts at: 3493 3860 3493 Size: 367 5796 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2705 3484 4939 618 Size: 325 217 627 918 779 1455 1842 Fragments arranged by size: 1842 1455 918 779 627 325 217 BanII G_rGCy'C Cuts at: 1391 2235 2831 3027 3108 3120 3482 1391 Size: 844 596 196 81 12 362 4072 Fragments arranged by size: 4072 844 596 362 196 81 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2681 928 Size: 971 782 4410 Fragments arranged by size: 4410 971 782 BbvCI CC'TCA_GC Cuts at: 2174 2742 2174 Size: 568 5595 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4354 4388 1903 Size: 34 2417 34 3678 Fragments arranged by size: 3678 2417 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 1548 Size: 6163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2995 4774 136 Size: 107 122 71 631 605 1323 1779 1525 Fragments arranged by size: 1779 1525 1323 631 605 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 3580 3580 Size: 6163 Bme1580I G_kGCm'C Cuts at: 1790 2235 2710 2982 4224 5380 1790 Size: 445 475 272 1242 1156 2573 Fragments arranged by size: 2573 1242 1156 475 445 272 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2969 3894 4852 Size: 416 53 83 399 24 1552 925 958 Cuts at: 4852 442 Size: 1753 Fragments arranged by size: 1753 1552 958 925 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2350 2350 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2901 3044 3479 4823 1239 Size: 1662 143 435 1344 2579 Fragments arranged by size: 2579 1662 1344 435 143 Bpu10I CC'TnA_GC Cuts at: 2174 2742 3096 3102 3114 2174 Size: 568 354 6 12 5223 Fragments arranged by size: 5223 568 354 12 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4288 5153 5451 4288 Size: 865 298 5000 Fragments arranged by size: 5000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 2271 4826 882 Size: 617 543 229 2555 2219 Fragments arranged by size: 2555 2219 617 543 229 BsaAI yAC'GTr Cuts at: 493 1204 3954 493 Size: 711 2750 2702 Fragments arranged by size: 2750 2702 711 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2681 3970 4352 4987 Size: 53 83 186 936 1148 1289 382 635 Cuts at: 4987 275 Size: 1451 Fragments arranged by size: 1451 1289 1148 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3355 4596 4994 5268 5415 5826 3355 Size: 1241 398 274 147 411 3692 Fragments arranged by size: 3692 1241 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5938 Fragments arranged by size: 5938 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2759 2783 2839 2890 2919 3035 3164 Size: 1021 552 24 56 51 29 116 129 Cuts at: 3164 3212 1186 Size: 48 4137 Fragments arranged by size: 4137 1021 552 129 116 56 51 48 29 24 BseYI C'CCAG_C Cuts at: 3028 3263 3269 3275 5366 5862 3028 Size: 235 6 6 2091 496 3329 Fragments arranged by size: 3329 2091 496 235 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 1163 Size: 573 580 5010 Fragments arranged by size: 5010 580 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3207 4224 4309 5380 3207 Size: 1017 85 1071 3990 Fragments arranged by size: 3990 1071 1017 85 BsmI GAATG_Cn' Cuts at: 3674 3767 3674 Size: 93 6070 BsmBI CGTCTCn'nnnn_ Cuts at: 2537 2537 Size: 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2710 2831 2982 3027 3108 3120 Size: 399 445 475 121 151 45 81 12 Cuts at: 3120 3207 3482 4224 4309 5380 1391 Size: 87 275 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 475 445 399 275 151 121 87 85 81 45 12 BspEI T'CCGG_A Cuts at: 3355 5826 3355 Size: 2471 3692 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2773 3518 844 Size: 1929 745 3489 Fragments arranged by size: 3489 1929 745 BsrBI CCG'CTC Cuts at: 1394 1823 4050 1394 Size: 429 2227 3507 Fragments arranged by size: 3507 2227 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4658 4832 65 Size: 1183 3410 174 1396 Fragments arranged by size: 3410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2481 2875 4807 4994 1700 Size: 110 671 394 1932 187 2869 Fragments arranged by size: 2869 1932 671 394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6163 BssSI C'ACGA_G Cuts at: 2466 2555 4223 5235 2466 Size: 89 1668 1012 3394 Fragments arranged by size: 3394 1668 1012 89 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6163 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstXI CCAn_nnnn'nTGG Cuts at: 2252 2252 Size: 6163 BstYI r'GATC_y Cuts at: 1972 2074 3294 3493 3860 4245 4262 5714 5800 Size: 102 1220 199 367 385 17 1452 86 Cuts at: 5800 5812 6158 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1220 385 367 346 199 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2832 2832 Size: 6163 BtgI C'CryG_G Cuts at: 513 1065 2043 2245 2710 2736 3415 513 Size: 552 978 202 465 26 679 3261 Fragments arranged by size: 3261 978 679 552 465 202 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5424 Fragments arranged by size: 5424 683 56 BtsI GCAGTG_nn' Cuts at: 2304 2882 3679 4473 4493 2304 Size: 578 797 794 20 3974 Fragments arranged by size: 3974 797 794 578 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DraIII CAC_nnn'GTG Cuts at: 2977 2977 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2479 2590 2787 Size: 54 1425 50 275 18 649 111 197 Cuts at: 2787 3418 3610 4499 6128 8 Size: 631 192 889 1629 43 Fragments arranged by size: 1629 1425 889 649 631 275 197 192 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2341 2744 2760 2856 3066 3437 4093 1353 Size: 988 403 16 96 210 371 656 3423 Fragments arranged by size: 3423 988 656 403 371 210 96 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4750 5124 5270 387 Size: 1064 138 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2738 2901 3044 3234 3479 4226 4823 Size: 393 1106 163 143 190 245 747 597 Cuts at: 4823 5610 5878 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1106 787 747 597 393 268 245 190 163 143 EcoO109I rG'GnC_Cy Cuts at: 2231 2653 2231 Size: 422 5741 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FspI TGC'GCA Cuts at: 4669 4669 Size: 6163 HaeII r_GCGC'y Cuts at: 1678 2631 5310 1678 Size: 953 2679 2531 Fragments arranged by size: 2679 2531 953 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 4804 4836 4878 Size: 32 135 32 638 32 2562 32 42 Cuts at: 4878 4910 1373 Size: 32 2626 Fragments arranged by size: 2626 2562 638 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3507 3753 669 Size: 988 281 1569 246 3079 Fragments arranged by size: 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 3452 748 Size: 2704 3459 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5277 5461 1305 Size: 3972 184 2007 Fragments arranged by size: 3972 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2592 2789 3420 10 Size: 54 1425 343 760 197 631 2753 Fragments arranged by size: 2753 1425 760 631 343 197 54 MslI CAynn'nnrTG Cuts at: 518 1166 2250 2310 3380 4121 4480 4639 518 Size: 648 1084 60 1070 741 359 159 2042 Fragments arranged by size: 2042 1084 1070 741 648 359 159 60 MspA1I CmG'CkG Cuts at: 1606 2173 3302 3579 4256 5404 5649 1606 Size: 567 1129 277 677 1148 245 2120 Fragments arranged by size: 2120 1148 1129 677 567 277 245 NcoI C'CATG_G Cuts at: 513 1065 2245 2736 3415 513 Size: 552 1180 491 679 3261 Fragments arranged by size: 3261 1180 679 552 491 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 3521 5066 1132 Size: 97 2292 1545 2229 Fragments arranged by size: 2292 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2053 1348 Size: 705 5458 PciI A'CATG_T Cuts at: 5062 5062 Size: 6163 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6163 PfoI T'CCnGG_A Cuts at: 3006 3006 Size: 6163 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 2424 2670 3733 2424 Size: 246 1063 4854 Fragments arranged by size: 4854 1063 246 PspOMI G'GGCC_C Cuts at: 2231 2231 Size: 6163 PspXI vC'TCGA_Gb Cuts at: 3206 3206 Size: 6163 PstI C_TGCA'G Cuts at: 830 3515 830 Size: 2685 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 PvuII CAG'CTG Cuts at: 2173 3302 2173 Size: 1129 5034 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SapI GCTCTTCn'nnn_ Cuts at: 2856 3066 2856 Size: 210 5953 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 2016 3511 4646 5327 5518 826 Size: 219 971 1495 1135 681 191 1471 Fragments arranged by size: 1495 1471 1135 971 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 3490 1872 Size: 1618 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3206 4267 5168 5430 820 Size: 197 926 1263 1061 901 262 1553 Fragments arranged by size: 1553 1263 1061 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 2909 3057 1517 Size: 1392 148 4623 Fragments arranged by size: 4623 1392 148 StyI C'CwwG_G Cuts at: 513 1065 2245 2577 2736 3041 3322 3415 3602 Size: 552 1180 332 159 305 281 93 187 Cuts at: 3602 513 Size: 3074 Fragments arranged by size: 3074 1180 552 332 305 281 187 159 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2295 2628 2988 4190 4207 4360 4545 Size: 1255 715 333 360 1202 17 153 185 Cuts at: 4545 4990 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1202 715 445 360 333 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4409 6121 96 Size: 274 80 33 51 1443 2432 1712 138 Fragments arranged by size: 2432 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6123 6155 6123 Size: 32 6131 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6163 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3422 3422 Size: 6163 XhoI C'TCGA_G Cuts at: 1943 3206 1943 Size: 1263 4900 XmaI C'CCGG_G Cuts at: 1870 3488 1870 Size: 1618 4545 XmnI GAAnn'nnTTC Cuts at: 4292 4292 Size: 6163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI