Restriction map for
pFN21AB7742
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MseI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 1350 TCCGGA T^CCGGA
AcsI 721 AAATTT [GA]^AATT[CT]
AcyI 675 GACGCC G[GA]^CG[CT]C
AocI 826 CCTGAGG CC^T[ACGT]AGG
Aor51HI 622 AGCGCT AGC^GCT
ApaI 226 GGGCCC GGGCC^C
ApoI 721 AAATTT [GA]^AATT[CT]
AspHI 1198 GTGCTC G[AT]GC[AT]^C
AvaII 606 GGACC G^G[AT]CC
AvrII 1036 CCTAGG C^CTAGG
BanI 700 GGTGCC G^G[CT][GA]CC
BbsI 669 GAAGAC GAAGAC(2/6)
BglI 984 GCCCATTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1036 CCTAGG C^CTAGG
BpuAI 669 GAAGAC GAAGAC(2/6)
BsaHI 675 GACGCC G[GA]^CG[CT]C
BsaWI 1350 TCCGGA [AT]^CCGG[AT]
BseAI 1350 TCCGGA T^CCGGA
BsgI 290 GTGCAG GTGCAG(16/14)
BsiHKAI 1198 GTGCTC G[AT]GC[AT]^C
BsmBI 538 GAGACG CGTCTC(1/5)
BspEI 1350 TCCGGA T^CCGGA
BspMI 777 GCAGGT ACCTGC(4/8)
BstXI 240 CCATGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 826 CCTGAGG CC^T[ACGT]AGG
Cac8I 861 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 623 GCGC GCG^C
DraIII 967 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 622 AGCGCT AGC^GCT
Eco81I 826 CCTGAGG CC^T[ACGT]AGG
FauI 1398 GCGGG CCCGC(4/6)
HaeII 622 AGCGCT [GA]GCGC^[CT]
HhaI 623 GCGC GCG^C
Hin1I 675 GACGCC G[GA]^CG[CT]C
HinP1I 623 GCGC G^CGC
HindIII 1447 AAGCTT A^AGCTT
HphI 52 GGTGA GGTGA(8/7)
MaeIII 811 GTGAC ^GT[ACGT]AC
MroI 1350 TCCGGA T^CCGGA
PaeR7I 1201 CTCGAG C^TCGAG
SfcI 11 CTGTAG C^T[GA][CT]AG
SmlI 1201 CTCGAG C^T[CT][GA]AG
Tsp45I 811 GTGAC ^GT[GC]AC
XcmI 1410 CCATGGCCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1201 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 689 CCGC CCGC(-3/-1)
1398 GCGG
BbvCI 168 GCTGAGG CCTCAGC(-5/-2)
736 GCTGAGG
BcnI 1002 CCCGG CC^[GC]GG
1254 CCCGG
BfaI 695 CTAG C^TAG
1037 CTAG
BsaI 43 GAGACC GGTCTC(1/5)
272 GAGACC
BsrFI 476 GCCGGT [GA]^CCGG[CT]
870 ACCGGC
BssSI 550 CACGAG CACGAG(-5/-1)
461 CTCGTG
BstYI 69 AGATCC [GA]^GATC[CT]
1289 GGATCT
Cfr10I 476 GCCGGT [GA]^CCGG[CT]
870 ACCGGC
DraII 225 AGGGCCC [GA]G^G[ACGT]CC[CT]
647 AGGCCCC
EcoO109I 225 AGGGCCC [GA]G^G[ACGT]CC[CT]
647 AGGCCCC
HgaI 140 GACGC GACGC(5/10)
675 GACGC
MaeI 695 CTAG C^TAG
1037 CTAG
MflI 69 AGATCC [GA]^GATC[CT]
1289 GGATCT
MspA1I 166 CAGCTG C[AC]G^C[GT]G
1295 CAGCTG
NciI 1002 CCCGG CC^[GC]GG
1254 CCCGG
PvuII 166 CAGCTG CAG^CTG
1295 CAGCTG
SapI 856 GAAGAGC GCTCTTC(1/4)
1066 GAAGAGC
SfaNI 343 GATGC GCATC(5/9)
235 GCATC
StuI 902 AGGCCT AGG^CCT
1050 AGGCCT
TaqI 128 TCGA T^CGA
1202 TCGA
TfiI 596 GAATC G^A[AT]TC
1213 GATTC
Tsp509I 722 AATT ^AATT
1438 AATT
TthHB8I 128 TCGA T^CGA
1202 TCGA
XhoII 69 AGATCC [GA]^GATC[CT]
1289 GGATCT
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AvaI
BalI BanII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeII MboI
MboII MluNI MnlI MscI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI StyI TseI TspRI