SPG20 ORF clone FHC00108

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00108

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Clone info.

Species

human

Clone Name

pFN21AA0610 Sequence (with vector) Restriction Map

Product ID

FHC00108

Flexi Clone PID

FXC00108

ORF Sequence

DNA sequence (1998 bp)
Protein sequence (666 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385362]

Gene

KIAA0610

Symbol

SPG20

Description

spastic paraplegia 20 (Troyer syndrome), transcript variant 1

Original Clone ID

hh01733b

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1998 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 666 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9356.1 6e-203 100.0 SPG20 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007330 20 95 PF04212 MIT IPR009686 415 615 PF06911 Senescence/spartin-associated HMMSmart IPR007330 16 94 SM00745 MIT
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005266374 666 275900,607111 100.0 100.0 XP_011533314 666 275900,607111 100.0 100.0 NP_001135766 666 275900,607111 100.0 100.0 NP_055902 666 275900,607111 100.0 100.0 XP_005266372 666 275900,607111 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0610 Download

>KIAA0610  1998 bp
ATGGAGCAAGAGCCACAAAATGGAGAACCTGCTGAAATTAAGATCATCAGAGAAGCATAT
AAGAAGGCCTTTTTATTTGTTAACAAAGGTCTGAATACAGATGAATTAGGTCAGAAGGAA
GAAGCAAAGAACTACTATAAGCAAGGAATAGGACACCTGCTCAGAGGGATCAGCATTTCA
TCAAAAGAGTCTGAACACACAGGTCCTGGGTGGGAATCTGCTAGACAGATGCAACAGAAA
ATGAAAGAAACTCTACAGAATGTACGCACCAGGCTGGAAATTCTAGAGAAGGGTCTTGCC
ACTTCTCTGCAGAATGATCTTCAGGAGGTGCCCAAGTTATATCCAGAATTTCCACCTAAA
GACATGTGTGAAAAATTACCAGAGCCTCAGTCTTTTAGTTCAGCTCCTCAGCATGCTGAA
GTAAATGGAAACACCTCAACTCCAAGTGCAGGGGCAGTTGCTGCACCTGCTTCTCTGTCT
TTACCATCACAAAGTTGTCCAGCAGAAGCTCCTCCTGCTTATACTCCTCAAGCTGCTGAA
GGTCACTACACTGTATCCTATGGAACAGATTCTGGGGAGTTTTCATCAGTTGGAGAGGAG
TTTTATAGGAATCATTCTCAGCCACCGCCTCTTGAGACCTTAGGGCTGGATGCAGATGAA
TTGATTTTGATACCAAATGGAGTACAGATTTTTTTTGTAAATCCTGCAGGGGAGGTTAGT
GCACCTTCGTATCCTGGGTACCTTCGAATTGTGAGGTTTTTGGATAATTCTCTCGATACG
GTTCTAAACCGTCCTCCCGGGTTTCTTCAGGTTTGTGACTGGTTATATCCTCTAGTTCCT
GATAGATCTCCGGTTCTGAAATGTACTGCGGGAGCCTACATGTTTCCTGATACAATGCTA
CAAGCAGCAGGATGCTTTGTGGGGGTCGTCCTGTCCTCTGAGTTACCAGAGGATGATAGA
GAGCTCTTTGAGGATCTGTTAAGGCAAATGTCTGACCTTCGGCTCCAGGCCAACTGGAAC
AGAGCAGAAGAAGAAAATGAATTCCAAATCCCTGGAAGAACTAGACCCTCCTCTGACCAA
CTAAAAGAAGCCTCTGGCACTGATGTGAAACAGTTGGACCAAGGCAATAAGGATGTACGT
CATAAAGGAAAACGTGGAAAAAGGGCTAAAGATACTTCAAGTGAAGAAGTTAACCTGAGT
CACATTGTACCATGTGAGCCAGTTCCAGAAGAAAAGCCAAAAGAATTACCTGAATGGAGT
GAAAAAGTGGCTCACAACATTTTGTCAGGTGCTTCCTGGGTGAGTTGGGGTTTAGTCAAA
GGTGCTGAGATTACTGGTAAGGCAATCCAGAAAGGTGCTTCTAAACTCCGAGAGCGGATT
CAACCAGAAGAAAAACCCGTGGAAGTTAGTCCAGCTGTCACCAAGGGACTTTATATAGCG
AAGCAAGCTACAGGAGGAGCAGCAAAAGTCAGTCAGTTCCTGGTTGATGGAGTTTGCACT
GTAGCAAATTGCGTTGGAAAAGAACTAGCTCCACATGTCAAGAAGCATGGAAGCAAACTT
GTTCCAGAATCTCTTAAAAAAGACAAAGATGGGAAATCTCCTCTGGATGGTGCTATGGTT
GTAGCAGCAAGTAGTGTTCAAGGATTTTCAACTGTCTGGCAAGGATTGGAATGTGCAGCT
AAATGCATCGTTAACAATGTTTCAGCAGAAACTGTACAAACTGTCAGATACAAATACGGA
TATAATGCAGGAGAAGCTACCCACCATGCGGTGGATTCTGCGGTCAATGTTGGCGTAACT
GCCTACAATATTAACAACATTGGTATCAAAGCAATGGTGAAGAAAACTGCAACACAAACA
GGACACACTCTCCTTGAGGACTATCAGATAGTTGATAATTCTCAGAGGGAAAATCAAGAA
GGAGCAGCAAATGTCAACGTGAGAGGGGAGAAGGATGAGCAGACGAAGGAAGTAAAGGAG
GCAAAGAAGAAAGATAAA

ORF Protein

Cloned ORF protein sequence for pFN21AA0610 Download

>KIAA0610  666 aa
MEQEPQNGEPAEIKIIREAYKKAFLFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIS
SKESEHTGPGWESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPK
DMCEKLPEPQSFSSAPQHAEVNGNTSTPSAGAVAAPASLSLPSQSCPAEAPPAYTPQAAE
GHYTVSYGTDSGEFSSVGEEFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVS
APSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTAGAYMFPDTML
QAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEEENEFQIPGRTRPSSDQ
LKEASGTDVKQLDQGNKDVRHKGKRGKRAKDTSSEEVNLSHIVPCEPVPEEKPKELPEWS
EKVAHNILSGASWVSWGLVKGAEITGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYIA
KQATGGAAKVSQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDKDGKSPLDGAMV
VAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGYNAGEATHHAVDSAVNVGVT
AYNINNIGIKAMVKKTATQTGHTLLEDYQIVDNSQRENQEGAANVNVRGEKDEQTKEVKE
AKKKDK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0610 Download

>pFN21AA0610  6703 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCAAGAGCCACAAAATGGAGAACCTGCTGA
AATTAAGATCATCAGAGAAGCATATAAGAAGGCCTTTTTATTTGTTAACAAAGGTCTGAA
TACAGATGAATTAGGTCAGAAGGAAGAAGCAAAGAACTACTATAAGCAAGGAATAGGACA
CCTGCTCAGAGGGATCAGCATTTCATCAAAAGAGTCTGAACACACAGGTCCTGGGTGGGA
ATCTGCTAGACAGATGCAACAGAAAATGAAAGAAACTCTACAGAATGTACGCACCAGGCT
GGAAATTCTAGAGAAGGGTCTTGCCACTTCTCTGCAGAATGATCTTCAGGAGGTGCCCAA
GTTATATCCAGAATTTCCACCTAAAGACATGTGTGAAAAATTACCAGAGCCTCAGTCTTT
TAGTTCAGCTCCTCAGCATGCTGAAGTAAATGGAAACACCTCAACTCCAAGTGCAGGGGC
AGTTGCTGCACCTGCTTCTCTGTCTTTACCATCACAAAGTTGTCCAGCAGAAGCTCCTCC
TGCTTATACTCCTCAAGCTGCTGAAGGTCACTACACTGTATCCTATGGAACAGATTCTGG
GGAGTTTTCATCAGTTGGAGAGGAGTTTTATAGGAATCATTCTCAGCCACCGCCTCTTGA
GACCTTAGGGCTGGATGCAGATGAATTGATTTTGATACCAAATGGAGTACAGATTTTTTT
TGTAAATCCTGCAGGGGAGGTTAGTGCACCTTCGTATCCTGGGTACCTTCGAATTGTGAG
GTTTTTGGATAATTCTCTCGATACGGTTCTAAACCGTCCTCCCGGGTTTCTTCAGGTTTG
TGACTGGTTATATCCTCTAGTTCCTGATAGATCTCCGGTTCTGAAATGTACTGCGGGAGC
CTACATGTTTCCTGATACAATGCTACAAGCAGCAGGATGCTTTGTGGGGGTCGTCCTGTC
CTCTGAGTTACCAGAGGATGATAGAGAGCTCTTTGAGGATCTGTTAAGGCAAATGTCTGA
CCTTCGGCTCCAGGCCAACTGGAACAGAGCAGAAGAAGAAAATGAATTCCAAATCCCTGG
AAGAACTAGACCCTCCTCTGACCAACTAAAAGAAGCCTCTGGCACTGATGTGAAACAGTT
GGACCAAGGCAATAAGGATGTACGTCATAAAGGAAAACGTGGAAAAAGGGCTAAAGATAC
TTCAAGTGAAGAAGTTAACCTGAGTCACATTGTACCATGTGAGCCAGTTCCAGAAGAAAA
GCCAAAAGAATTACCTGAATGGAGTGAAAAAGTGGCTCACAACATTTTGTCAGGTGCTTC
CTGGGTGAGTTGGGGTTTAGTCAAAGGTGCTGAGATTACTGGTAAGGCAATCCAGAAAGG
TGCTTCTAAACTCCGAGAGCGGATTCAACCAGAAGAAAAACCCGTGGAAGTTAGTCCAGC
TGTCACCAAGGGACTTTATATAGCGAAGCAAGCTACAGGAGGAGCAGCAAAAGTCAGTCA
GTTCCTGGTTGATGGAGTTTGCACTGTAGCAAATTGCGTTGGAAAAGAACTAGCTCCACA
TGTCAAGAAGCATGGAAGCAAACTTGTTCCAGAATCTCTTAAAAAAGACAAAGATGGGAA
ATCTCCTCTGGATGGTGCTATGGTTGTAGCAGCAAGTAGTGTTCAAGGATTTTCAACTGT
CTGGCAAGGATTGGAATGTGCAGCTAAATGCATCGTTAACAATGTTTCAGCAGAAACTGT
ACAAACTGTCAGATACAAATACGGATATAATGCAGGAGAAGCTACCCACCATGCGGTGGA
TTCTGCGGTCAATGTTGGCGTAACTGCCTACAATATTAACAACATTGGTATCAAAGCAAT
GGTGAAGAAAACTGCAACACAAACAGGACACACTCTCCTTGAGGACTATCAGATAGTTGA
TAATTCTCAGAGGGAAAATCAAGAAGGAGCAGCAAATGTCAACGTGAGAGGGGAGAAGGA
TGAGCAGACGAAGGAAGTAAAGGAGGCAAAGAAGAAAGATAAAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT