(Circular) (Six-base) MAPSORT of: pFN21AA0610.seq Check: 4892 from: 1 to: 6703 pFN21AA0610 6703 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2169 2479 2169 Size: 310 6393 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4513 278 Size: 53 83 186 936 2977 2468 Fragments arranged by size: 2977 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4046 1937 Size: 2109 4594 Acc65I G'GTAC_C Cuts at: 2742 4024 2742 Size: 1282 5421 AclI AA'CG_TT Cuts at: 1563 4830 5203 1563 Size: 3267 373 3063 Fragments arranged by size: 3267 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2442 2562 2795 4766 6150 6418 1632 Size: 677 133 120 233 1971 1384 268 1917 Fragments arranged by size: 1971 1917 1384 677 268 233 133 120 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6506 AflIII A'CryG_T Cuts at: 1203 2367 2883 3538 5602 6579 1203 Size: 1164 516 655 2064 977 1327 Fragments arranged by size: 2064 1327 1164 977 655 516 AgeI A'CCGG_T Cuts at: 5534 5534 Size: 6703 AhdI GACnn_n'nnGTC Cuts at: 5432 5432 Size: 6703 AleI CACnn'nnGTG Cuts at: 1166 3107 1166 Size: 1941 4762 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2932 3703 1184 Size: 1748 771 4184 Fragments arranged by size: 4184 1748 771 AlwNI CAG_nnn'CTG Cuts at: 2210 2465 2576 3484 3716 4446 6018 2210 Size: 255 111 908 232 730 1572 2895 Fragments arranged by size: 2895 1572 908 730 255 232 111 ApaLI G'TGCA_C Cuts at: 2724 4760 5916 2724 Size: 2036 1156 3511 Fragments arranged by size: 3511 2036 1156 ApoI r'AATT_y Cuts at: 1493 2283 2351 3044 4012 4238 4390 6499 1493 Size: 790 68 693 968 226 152 2109 1697 Fragments arranged by size: 2109 1697 968 790 693 226 152 68 AseI AT'TA_AT Cuts at: 160 1032 5257 160 Size: 872 4225 1606 Fragments arranged by size: 4225 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6703 AvaI C'yCGr_G Cuts at: 1870 1943 2801 4028 1870 Size: 73 858 1227 4545 Fragments arranged by size: 4545 1227 858 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2717 2750 3811 3844 2717 Size: 33 1061 33 5576 Fragments arranged by size: 5576 1061 33 33 BamHI G'GATC_C Cuts at: 4033 4400 4033 Size: 367 6336 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2332 2742 4024 5479 618 Size: 325 217 627 545 410 1282 1455 1842 Fragments arranged by size: 1842 1455 1282 627 545 410 325 217 BanII G_rGCy'C Cuts at: 1391 2970 4022 1391 Size: 1579 1052 4072 Fragments arranged by size: 4072 1579 1052 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5732 BbvCI CC'TCA_GC Cuts at: 2412 2412 Size: 6703 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4894 4928 1903 Size: 34 2957 34 3678 Fragments arranged by size: 3678 2957 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2569 2745 4595 5805 2569 Size: 176 1850 1210 3467 Fragments arranged by size: 3467 1850 1210 176 BclI T'GATC_A Cuts at: 1548 1548 Size: 6703 BfrBI ATG'CAT Cuts at: 3690 3690 Size: 6703 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5314 136 Size: 107 122 71 631 605 3642 1525 Fragments arranged by size: 3642 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2849 6698 2849 Size: 3849 2854 BlpI GC'TnA_GC Cuts at: 4120 4120 Size: 6703 Bme1580I G_kGCm'C Cuts at: 1790 2337 2728 4764 5920 1790 Size: 547 391 2036 1156 2573 Fragments arranged by size: 2573 2036 1156 547 391 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6703 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4434 5392 442 Size: 416 53 83 399 24 3017 958 1753 Fragments arranged by size: 3017 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6703 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2993 5363 1239 Size: 1754 2370 2579 Fragments arranged by size: 2579 2370 1754 Bpu10I CC'TnA_GC Cuts at: 2412 2412 Size: 6703 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2517 2657 3899 4828 5693 5991 2517 Size: 140 1242 929 865 298 3229 Fragments arranged by size: 3229 1242 929 865 298 140 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2633 5366 882 Size: 617 1134 2733 2219 Fragments arranged by size: 2733 2219 1134 617 BsaAI yAC'GTr Cuts at: 493 1204 4494 493 Size: 711 3290 2702 Fragments arranged by size: 3290 2702 711 BsaBI GATnn'nnATC Cuts at: 6697 6697 Size: 6703 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4510 4892 5527 275 Size: 53 83 186 936 2977 382 635 1451 Fragments arranged by size: 2977 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2854 5136 5534 5808 5955 6366 2854 Size: 2282 398 274 147 411 3191 Fragments arranged by size: 3191 2282 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2428 2458 3858 3888 1178 Size: 30 165 30 1025 30 1400 30 3993 Fragments arranged by size: 3993 1400 1025 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2400 2505 2520 2615 3064 3474 3594 1186 Size: 1214 105 15 95 449 410 120 4295 Fragments arranged by size: 4295 1214 449 410 120 105 95 15 BseYI C'CCAG_C Cuts at: 5906 6402 5906 Size: 496 6207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2451 2472 3699 1163 Size: 573 715 21 1227 4167 Fragments arranged by size: 4167 1227 715 573 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4153 4914 5942 1540 Size: 275 186 2152 761 1028 2301 Fragments arranged by size: 2301 2152 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2728 2970 4764 4849 5920 2728 Size: 242 1794 85 1071 3511 Fragments arranged by size: 3511 1794 1071 242 85 BsmI GAATG_Cn' Cuts at: 4214 4307 4214 Size: 93 6610 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2728 2970 4022 4764 4849 5920 Size: 399 547 391 242 1052 742 85 1071 Cuts at: 5920 1391 Size: 2174 Fragments arranged by size: 2174 1071 1052 742 547 399 391 242 85 BspEI T'CCGG_A Cuts at: 6366 6366 Size: 6703 BspHI T'CATG_A Cuts at: 4592 6322 4592 Size: 1730 4973 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2041 2169 2479 4058 844 Size: 1197 128 310 1579 3489 Fragments arranged by size: 3489 1579 1197 310 128 BsrBI CCG'CTC Cuts at: 1394 1823 3379 4590 1394 Size: 429 1556 1211 3507 Fragments arranged by size: 3507 1556 1211 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3843 5198 5372 65 Size: 1183 2595 1355 174 1396 Fragments arranged by size: 2595 1396 1355 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5347 5534 1700 Size: 110 3537 187 2869 Fragments arranged by size: 3537 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3718 96 Size: 3622 3081 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6703 BssSI C'ACGA_G Cuts at: 4763 5775 4763 Size: 1012 5691 BstAPI GCAn_nnn'nTGC Cuts at: 794 2465 794 Size: 1671 5032 BstBI TT'CG_AA Cuts at: 2749 5507 2749 Size: 2758 3945 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6703 BstXI CCAn_nnnn'nTGG Cuts at: 3056 3056 Size: 6703 BstYI r'GATC_y Cuts at: 1972 2849 2977 4033 4400 4785 4802 6254 6340 Size: 877 128 1056 367 385 17 1452 86 Cuts at: 6340 6352 6698 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1056 877 385 367 346 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2644 2644 Size: 6703 BtgI C'CryG_G Cuts at: 513 1065 2004 3402 513 Size: 552 939 1398 3814 Fragments arranged by size: 3814 1398 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5964 Fragments arranged by size: 5964 683 56 BtsI GCAGTG_nn' Cuts at: 4219 5013 5033 4219 Size: 794 20 5889 Fragments arranged by size: 5889 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6703 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6668 DraI TTT'AAA Cuts at: 4007 4007 Size: 6703 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5710 809 Size: 507 4394 1802 Fragments arranged by size: 4394 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4150 5039 6668 Size: 54 1425 50 275 18 2320 889 1629 Cuts at: 6668 8 Size: 43 Fragments arranged by size: 2320 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4150 1537 Size: 275 2338 4090 Fragments arranged by size: 4090 2338 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4633 1353 Size: 3280 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5290 5664 5810 387 Size: 1064 138 3701 374 146 1280 Fragments arranged by size: 3701 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2968 2968 Size: 6703 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2309 2442 2562 2795 2993 4766 5363 Size: 393 677 133 120 233 198 1773 597 Cuts at: 5363 6150 6418 1239 Size: 787 268 1524 Fragments arranged by size: 1773 1524 787 677 597 393 268 233 198 133 120 EcoO109I rG'GnC_Cy Cuts at: 2207 2207 Size: 6703 EcoRI G'AATT_C Cuts at: 3044 4012 4390 3044 Size: 968 378 5357 Fragments arranged by size: 5357 968 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2089 2089 Size: 6703 FspI TGC'GCA Cuts at: 5209 5209 Size: 6703 HaeII r_GCGC'y Cuts at: 1678 5850 1678 Size: 4172 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2378 2410 2573 Size: 32 135 32 576 32 198 32 163 Cuts at: 2573 2605 3584 3616 3858 3890 3901 3933 5344 Size: 32 979 32 242 32 11 32 1411 Cuts at: 5344 5376 5418 5450 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1411 979 576 242 198 163 135 42 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 2086 3196 3697 3940 4047 4293 Size: 988 281 148 1110 501 243 107 246 Cuts at: 4293 669 Size: 3079 Fragments arranged by size: 3079 1110 988 501 281 246 243 148 107 HindIII A'AGCT_T Cuts at: 748 748 Size: 6703 HpaI GTT'AAC Cuts at: 2086 3196 3697 4293 2086 Size: 1110 501 596 4496 Fragments arranged by size: 4496 1110 596 501 KpnI G_GTAC'C Cuts at: 2746 4028 2746 Size: 1282 5421 MfeI C'AATT_G Cuts at: 4302 4302 Size: 6703 MluI A'CGCG_T Cuts at: 6579 6579 Size: 6703 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2575 3099 3499 5817 6001 1305 Size: 1270 524 400 2318 184 2007 Fragments arranged by size: 2318 2007 1270 524 400 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4881 Fragments arranged by size: 4881 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3107 4661 5020 5179 518 Size: 648 1941 1554 359 159 2042 Fragments arranged by size: 2042 1941 1554 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3419 4119 4796 5944 6189 1606 Size: 1813 700 677 1148 245 2120 Fragments arranged by size: 2120 1813 1148 700 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5212 Fragments arranged by size: 5212 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6703 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6703 NotI GC'GGCC_GC Cuts at: 4150 4150 Size: 6703 NsiI A_TGCA'T Cuts at: 3692 3692 Size: 6703 NspI r_CATG'y Cuts at: 1132 1229 2371 2420 2887 3542 4061 5606 1132 Size: 97 1142 49 467 655 519 1545 2229 Fragments arranged by size: 2229 1545 1142 655 519 467 97 49 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6703 PciI A'CATG_T Cuts at: 2367 2883 3538 5602 2367 Size: 516 655 2064 3468 Fragments arranged by size: 3468 2064 655 516 PflMI CCAn_nnn'nTGG Cuts at: 1108 2024 3775 1108 Size: 916 1751 4036 Fragments arranged by size: 4036 1751 916 PmeI GTTT'AAAC Cuts at: 4007 4007 Size: 6703 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4772 4804 6285 6317 4772 Size: 32 1481 32 5158 Fragments arranged by size: 5158 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2207 2207 Size: 6703 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6703 PsiI TTA'TAA Cuts at: 4273 4273 Size: 6703 PstI C_TGCA'G Cuts at: 830 2316 2713 4055 830 Size: 1486 397 1342 3478 Fragments arranged by size: 3478 1486 1342 397 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6703 PvuII CAG'CTG Cuts at: 3419 3419 Size: 6703 SacI G_AGCT'C Cuts at: 2970 2970 Size: 6703 SalI G'TCGA_C Cuts at: 1936 4045 1936 Size: 2109 4594 SbfI CC_TGCA'GG Cuts at: 2713 4055 2713 Size: 1342 5361 ScaI AGT'ACT Cuts at: 4951 6663 4951 Size: 1712 4991 SfcI C'TryA_G Cuts at: 826 1045 2258 2312 2709 3453 3504 4051 5186 Size: 219 1213 54 397 744 51 547 1135 Cuts at: 5186 5867 6058 826 Size: 681 191 1471 Fragments arranged by size: 1471 1213 1135 744 681 547 397 219 191 54 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6703 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6703 SmaI CCC'GGG Cuts at: 1872 2803 4030 1872 Size: 931 1227 4545 Fragments arranged by size: 4545 1227 931 SmlI C'TyrA_G Cuts at: 820 1017 1943 2532 2636 3878 4807 5708 5970 Size: 197 926 589 104 1242 929 901 262 Cuts at: 5970 820 Size: 1553 Fragments arranged by size: 1553 1242 929 926 901 589 262 197 104 SnaBI TAC'GTA Cuts at: 493 4494 493 Size: 4001 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6703 SphI G_CATG'C Cuts at: 1132 2420 4061 1132 Size: 1288 1641 3774 Fragments arranged by size: 3774 1641 1288 SspI AAT'ATT Cuts at: 5 52 3814 4627 5 Size: 47 3762 813 2081 Fragments arranged by size: 3762 2081 813 47 StuI AGG'CCT Cuts at: 1517 2072 1517 Size: 555 6148 StyI C'CwwG_G Cuts at: 513 1065 2004 3124 3426 4142 513 Size: 552 939 1120 302 716 3074 Fragments arranged by size: 3074 1120 939 716 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2202 3292 4730 4747 4900 5085 5530 Size: 1255 622 1090 1438 17 153 185 445 Cuts at: 5530 325 Size: 1498 Fragments arranged by size: 1498 1438 1255 1090 622 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2686 2867 3718 Size: 274 80 33 51 1443 709 181 851 Cuts at: 3718 4949 6661 96 Size: 1231 1712 138 Fragments arranged by size: 1712 1443 1231 851 709 274 181 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2428 2460 2907 2939 6663 6695 2428 Size: 32 447 32 3724 32 2436 Fragments arranged by size: 3724 2436 447 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6703 XbaI T'CTAG_A Cuts at: 2287 4039 2287 Size: 1752 4951 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6703 XmaI C'CCGG_G Cuts at: 1870 2801 4028 1870 Size: 931 1227 4545 Fragments arranged by size: 4545 1227 931 XmnI GAAnn'nnTTC Cuts at: 2572 4832 2572 Size: 2260 4443 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4511 276 Size: 53 83 186 936 2977 2468 Fragments arranged by size: 2977 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI