Restriction map for
pFN21AA0610
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SwaI
Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 737 GGTACC G^GTACC
Alw44I 719 GTGCAC G^TGCAC
AocI 638 CCTTAGG CC^T[ACGT]AGG
ApaLI 719 GTGCAC G^TGCAC
Asp700I 563 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 737 GGTACC G^GTACC
AvaI 796 CCCGGG C^[CT]CG[GA]G
BanII 961 GAGCTC G[GA]GC[CT]^C
BbvCI 406 CCTCAGC CCTCAGC(-5/-2)
BglII 844 AGATCT A^GATCT
BpmI 1003 CTCCAG CTGGAG(16/14)
BsaI 634 GAGACC GGTCTC(1/5)
BsaWI 849 TCCGGT [AT]^CCGG[AT]
BsmAI 634 GAGAC GTCTC(1/5)
BsmFI 1425 GGGAC GGGAC(10/14)
BsoBI 796 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1713 TGTACA T^GTACA
BsrBI 1372 GAGCGG CCGCTC(-3/-3)
BsrDI 1831 GCAATG GCAATG(2/0)
BsrGI 1713 TGTACA T^GTACA
BstAPI 454 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 743 TTCGAA TT^CGAA
BstXI 1044 CCAAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 638 CCTTAGG CC^T[ACGT]AGG
DraII 201 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1397 CCGTGG C^C[GA][CT]GG
Eco81I 638 CCTTAGG CC^T[ACGT]AGG
EcoO109I 201 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1039 GAATTC G^AATTC
EcoT22I 1683 ATGCAT ATGCA^T
FauI 868 GCGGG CCCGC(4/6)
KpnI 737 GGTACC GGTAC^C
MslI 1098 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1412 CAGCTG C[AC]G^C[GT]G
NsiI 1683 ATGCAT ATGCA^T
NspV 743 TTCGAA TT^CGAA
PpuMI 201 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1412 CAGCTG CAG^CTG
SacI 961 GAGCTC GAGCT^C
SbfI 703 CCTGCAGG CCTGCA^GG
SfuI 743 TTCGAA TT^CGAA
SmaI 796 CCCGGG CCC^GGG
SphI 411 GCATGC GCATG^C
Sse8387I 703 CCTGCAGG CCTGCA^GG
SspBI 1713 TGTACA T^GTACA
SspI 1807 AATATT AAT^ATT
StuI 65 AGGCCT AGG^CCT
XbaI 282 TCTAGA T^CTAGA
XmaCI 796 CCCGGG C^CCGGG
XmaI 796 CCCGGG C^CCGGG
XmnI 563 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 167 GGATC GGATC(4/5)
972 GGATC
AspHI 719 GTGCAC G[AT]GC[AT]^C
961 GAGCTC
AvaII 202 GGTCC G^G[AT]CC
1116 GGACC
BanI 327 GGTGCC G^G[CT][GA]CC
737 GGTACC
BciVI 553 GTATCC GTATCC(6/5)
729 GTATCC
BcnI 796 CCCGG CC^[GC]GG
797 CCGGG
BsiHKAI 719 GTGCAC G[AT]GC[AT]^C
961 GAGCTC
BstYI 844 AGATCT [GA]^GATC[CT]
972 GGATCT
Cac8I 411 GCATGC GC[ACGT]^[ACGT]GC
1443 GCAAGC
Cfr13I 202 GGTCC G^G[ACGT]CC
1116 GGACC
EcoT14I 1119 CCAAGG C^C[AT][AT]GG
1421 CCAAGG
HaeIII 66 GGCC GG^CC
1008 GGCC
HapII 797 CCGG C^CGG
850 CCGG
HpaII 797 CCGG C^CGG
850 CCGG
MflI 844 AGATCT [GA]^GATC[CT]
972 GGATCT
MspI 797 CCGG C^CGG
850 CCGG
NciI 796 CCCGG CC^[GC]GG
797 CCGGG
PflMI 13 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1764 CCATGCGGTGG
PleI 187 GAGTC GAGTC(4/5)
1197 GAGTC
PstI 307 CTGCAG CTGCA^G
704 CTGCAG
Sau96I 202 GGTCC G^G[ACGT]CC
1116 GGACC
StyI 1119 CCAAGG C^C[AT][AT]GG
1421 CCAAGG
TaqI 744 TCGA T^CGA
773 TCGA
TthHB8I 744 TCGA T^CGA
773 TCGA
Van91I 13 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1764 CCATGCGGTGG
XhoII 844 AGATCT [GA]^GATC[CT]
972 GGATCT
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI BbvI BfaI BmyI
BsaJI BseRI BsgI BsiYI BslI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI DdeI DpnII EcoRII
Fnu4HI FokI HincII HindII HinfI
HpaI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI