DCAF8 ORF clone FHC10767

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10767

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Clone info.

Species

human

Clone Name

pFN21AB3684 Sequence (with vector) Restriction Map

Product ID

FHC10767

Flexi Clone PID

FXC10767

ORF Sequence

DNA sequence (1791 bp)
Protein sequence (597 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587369]

Gene

KIBB3684

Symbol

DCAF8

Alias : GAN2, H326, WDR42A

Description

DDB1 and CUL4 associated factor 8, transcript variant 1

Original Clone ID

hk04023

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1791 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 597 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1200.1 6e-146 100.0 DCAF8 CCDS35222.1 1.1e-93 67.9 DCAF8L1 CCDS59162.1 9.3e-92 67.7 DCAF8L2 CCDS30933.1 9.2e-12 32.5 DCAF6 CCDS1267.2 9.4e-12 32.5 DCAF6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019781 187 221 PF00400 WD40 repeat IPR019781 233 264 PF00400 WD40 repeat IPR019781 435 463 PF00400 WD40 repeat IPR019781 480 505 PF00400 WD40 repeat HMMSmart IPR001680 182 221 SM00320 WD40 repeat IPR001680 224 266 SM00320 WD40 repeat IPR001680 270 312 SM00320 WD40 repeat IPR001680 320 360 SM00320 WD40 repeat IPR001680 375 415 SM00320 WD40 repeat IPR001680 421 463 SM00320 WD40 repeat IPR001680 466 506 SM00320 WD40 repeat ProfileScan IPR019782 189 221 PS50082 WD40 repeat 2 IPR017986 189 515 PS50294 WD40-repeat-containing domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056541 597 610100,615820 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3684 Download

>KIBB3684  1791 bp
ATGTCCAGCAAAGGGAGCAGCACAGATGGCAGAACAGACTTAGCTAATGGAAGCCTGTCT
AGCAGTCCAGAGGAGATGTCTGGAGCTGAAGAGGGGAGGGAGACATCCTCAGGCATTGAA
GTGGAGGCCTCAGACCTGAGTTTGAGCTTGACTGGGGATGATGGTGGCCCCAACCGCACC
AGCACAGAAAGTCGAGGCACAGACACAGAGAGCTCAGGTGAAGATAAGGACTCTGACAGC
ATGGAGGACACTGGTCATTACTCCATTAATGATGAAAATCGAGTCCATGACCGCTCAGAG
GAAGAGGAAGAGGAGGAAGAAGAGGAGGAAGAAGAGCAGCCTCGGCGCCGTGTACAGCGC
AAGCGGGCTAACCGTGACCAGGACTCATCAGATGATGAGCGGGCCCTAGAGGACTGGGTG
TCCTCAGAAACATCAGCTCTACCCCGACCTCGCTGGCAAGCCCTCCCTGCCCTTCGGGAG
CGGGAGCTGGGTTCAAGTGCCCGCTTTGTCTATGAGGCCTGTGGGGCAAGAGTCTTTGTG
CAGCGTTTCCGCCTGCAGCATGGGCTTGAGGGCCATACTGGTTGTGTCAATACCCTGCAC
TTTAACCAGCGCGGCACCTGGCTGGCCAGTGGCAGCGATGACCTGAAGGTGGTGGTGTGG
GATTGGGTACGGCGGCAGCCAGTACTGGACTTTGAGAGTGGCCACAAAAGTAATGTGTTC
CAGGCCAAGTTTCTTCCTAACAGTGGTGATTCTACTCTGGCCATGTGTGCCCGTGACGGG
CAGGTTCGAGTAGCAGAACTGTCTGCCACACAGTGTTGCAAGAATACAAAACGTGTGGCC
CAGCACAAGGGAGCGTCCCACAAGTTGGCACTGGAACCAGACTCTCCCTGTACGTTCTTA
TCTGCAGGTGAAGATGCAGTTGTTTTCACCATTGACCTGAGACAAGACCGCCCAGCGTCG
AAACTGGTGGTGACAAAAGAGAAAGAGAAGAAAGTGGGGCTGTATACGATCTATGTGAAT
CCTGCCAATACCCACCAGTTTGCAGTGGGTGGACGAGATCAGTTTGTAAGGATTTATGAC
CAGAGGAAAATTGATGAGAATGAGAACAATGGAGTACTCAAGAAGTTCTGTCCTCATCAC
CTGGTGAACAGTGAGTCCAAAGCAAACATCACCTGTCTTGTGTACAGCCACGACGGCACA
GAGCTCCTGGCCAGTTACAATGATGAAGACATTTACCTCTTCAACTCCTCTCACAGTGAT
GGGGCCCAGTATGTTAAGAGATACAAGGGCCACAGAAATAATGCCACAGTAAAAGGCGTC
AATTTCTATGGCCCCAAGAGTGAGTTTGTGGTGAGCGGTAGTGACTGTGGGCACATCTTC
CTCTGGGAGAAATCATCCTGCCAGATTATTCAGTTCATGGAGGGGGACAAGGGAGGCGTG
GTAAACTGTCTTGAGCCCCACCCTCACCTGCCTGTGCTGGCAACCAGTGGCCTAGACCAT
GATGTGAAGATCTGGGCACCCACAGCTGAAGCTTCCACTGAGCTGACAGGGTTAAAAGAT
GTGATTAAGAAGAACAAGCGGGAGCGGGATGAAGATAGCTTGCACCAAACTGACCTGTTT
GATAGTCACATGCTGTGGTTCCTTATGCATCACCTGAGACAGAGACGCCATCACCGGCGC
TGGCGAGAACCTGGGGTTGGGGCCACAGACGCGGACTCTGATGAGTCTCCCAGCTCCTCA
GACACATCGGACGAGGAGGAGGGCCCTGACCGGGTGCAGTGCATGCCATCT

ORF Protein

Cloned ORF protein sequence for pFN21AB3684 Download

>KIBB3684  597 aa
MSSKGSSTDGRTDLANGSLSSSPEEMSGAEEGRETSSGIEVEASDLSLSLTGDDGGPNRT
STESRGTDTESSGEDKDSDSMEDTGHYSINDENRVHDRSEEEEEEEEEEEEEQPRRRVQR
KRANRDQDSSDDERALEDWVSSETSALPRPRWQALPALRERELGSSARFVYEACGARVFV
QRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKVVVWDWVRRQPVLDFESGHKSNVF
QAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTKRVAQHKGASHKLALEPDSPCTFL
SAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVGLYTIYVNPANTHQFAVGGRDQFVRIYD
QRKIDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSD
GAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGV
VNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLF
DSHMLWFLMHHLRQRRHHRRWREPGVGATDADSDESPSSSDTSDEEEGPDRVQCMPS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3684 Download

>pFN21AB3684  6496 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCAGCAAAGGGAGCAGCACAGATGGCAGAAC
AGACTTAGCTAATGGAAGCCTGTCTAGCAGTCCAGAGGAGATGTCTGGAGCTGAAGAGGG
GAGGGAGACATCCTCAGGCATTGAAGTGGAGGCCTCAGACCTGAGTTTGAGCTTGACTGG
GGATGATGGTGGCCCCAACCGCACCAGCACAGAAAGTCGAGGCACAGACACAGAGAGCTC
AGGTGAAGATAAGGACTCTGACAGCATGGAGGACACTGGTCATTACTCCATTAATGATGA
AAATCGAGTCCATGACCGCTCAGAGGAAGAGGAAGAGGAGGAAGAAGAGGAGGAAGAAGA
GCAGCCTCGGCGCCGTGTACAGCGCAAGCGGGCTAACCGTGACCAGGACTCATCAGATGA
TGAGCGGGCCCTAGAGGACTGGGTGTCCTCAGAAACATCAGCTCTACCCCGACCTCGCTG
GCAAGCCCTCCCTGCCCTTCGGGAGCGGGAGCTGGGTTCAAGTGCCCGCTTTGTCTATGA
GGCCTGTGGGGCAAGAGTCTTTGTGCAGCGTTTCCGCCTGCAGCATGGGCTTGAGGGCCA
TACTGGTTGTGTCAATACCCTGCACTTTAACCAGCGCGGCACCTGGCTGGCCAGTGGCAG
CGATGACCTGAAGGTGGTGGTGTGGGATTGGGTACGGCGGCAGCCAGTACTGGACTTTGA
GAGTGGCCACAAAAGTAATGTGTTCCAGGCCAAGTTTCTTCCTAACAGTGGTGATTCTAC
TCTGGCCATGTGTGCCCGTGACGGGCAGGTTCGAGTAGCAGAACTGTCTGCCACACAGTG
TTGCAAGAATACAAAACGTGTGGCCCAGCACAAGGGAGCGTCCCACAAGTTGGCACTGGA
ACCAGACTCTCCCTGTACGTTCTTATCTGCAGGTGAAGATGCAGTTGTTTTCACCATTGA
CCTGAGACAAGACCGCCCAGCGTCGAAACTGGTGGTGACAAAAGAGAAAGAGAAGAAAGT
GGGGCTGTATACGATCTATGTGAATCCTGCCAATACCCACCAGTTTGCAGTGGGTGGACG
AGATCAGTTTGTAAGGATTTATGACCAGAGGAAAATTGATGAGAATGAGAACAATGGAGT
ACTCAAGAAGTTCTGTCCTCATCACCTGGTGAACAGTGAGTCCAAAGCAAACATCACCTG
TCTTGTGTACAGCCACGACGGCACAGAGCTCCTGGCCAGTTACAATGATGAAGACATTTA
CCTCTTCAACTCCTCTCACAGTGATGGGGCCCAGTATGTTAAGAGATACAAGGGCCACAG
AAATAATGCCACAGTAAAAGGCGTCAATTTCTATGGCCCCAAGAGTGAGTTTGTGGTGAG
CGGTAGTGACTGTGGGCACATCTTCCTCTGGGAGAAATCATCCTGCCAGATTATTCAGTT
CATGGAGGGGGACAAGGGAGGCGTGGTAAACTGTCTTGAGCCCCACCCTCACCTGCCTGT
GCTGGCAACCAGTGGCCTAGACCATGATGTGAAGATCTGGGCACCCACAGCTGAAGCTTC
CACTGAGCTGACAGGGTTAAAAGATGTGATTAAGAAGAACAAGCGGGAGCGGGATGAAGA
TAGCTTGCACCAAACTGACCTGTTTGATAGTCACATGCTGTGGTTCCTTATGCATCACCT
GAGACAGAGACGCCATCACCGGCGCTGGCGAGAACCTGGGGTTGGGGCCACAGACGCGGA
CTCTGATGAGTCTCCCAGCTCCTCAGACACATCGGACGAGGAGGAGGGCCCTGACCGGGT
GCAGTGCATGCCATCTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT