Restriction map for
pFN21AB3684
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I AlwI AlwNI
Aor51HI ApaLI ApoI AscI Asp700I
Asp718I AspI AvaI AvaII AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI BstBI BstEII BstPI BstXI
CelII ClaI CpoI DraI DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SspI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1002 GTATAC GT^[AC][GT]AC
AflIII 831 ACGTGT A^C[GA][CT]GT
AhdI 412 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 107 CCTCAGG CC^T[ACGT]AGG
AseI 265 ATTAAT AT^TAAT
AsnI 265 ATTAAT AT^TAAT
AspEI 412 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 344 GGCGCC GGCGC^C
BbsI 1225 GAAGAC GAAGAC(2/6)
BcnI 1770 CCGGG CC^[GC]GG
BglII 1508 AGATCT A^GATCT
BpmI 80 CTGGAG CTGGAG(16/14)
BpuAI 1225 GAAGAC GAAGAC(2/6)
BsaBI 271 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1662 GAGACG CGTCTC(1/5)
BsrFI 1673 ACCGGC [GA]^CCGG[CT]
Bst1107I 1002 GTATAC GTA^TAC
BstAPI 1776 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1508 AGATCT [GA]^GATC[CT]
BstZ17I 1002 GTATAC GTA^TAC
Bsu36I 107 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1673 ACCGGC [GA]^CCGG[CT]
Eam1105I 412 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 107 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1645 ATGCAT ATGCA^T
HindIII 1529 AAGCTT A^AGCTT
KasI 344 GGCGCC G^GCGCC
MamI 271 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1508 AGATCT [GA]^GATC[CT]
MspA1I 1523 CAGCTG C[AC]G^C[GT]G
NarI 344 GGCGCC GG^CGCC
NciI 1770 CCGGG CC^[GC]GG
NsiI 1645 ATGCAT ATGCA^T
PflMI 858 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 247 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 265 ATTAAT AT^TAAT
PvuII 1523 CAGCTG CAG^CTG
SapI 331 GAAGAGC GCTCTTC(1/4)
SexAI 1139 ACCTGGT A^CC[AT]GGT
SfoI 344 GGCGCC GGC^GCC
SgrAI 1672 CACCGGCG C[GA]^CCGG[CT]G
SphI 1781 GCATGC GCATG^C
Van91I 858 CCACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1508 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 610 CGCG CG^CG
1710 CGCG
AspHI 210 GAGCTC G[AT]GC[AT]^C
1201 GAGCTC
BglI 517 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1187 GCCACGACGGC
BsaJI 340 CCTCGG C^C[ACGT][ACGT]GG
1690 CCTGGG
BsiHKAI 210 GAGCTC G[AT]GC[AT]^C
1201 GAGCTC
BsmFI 1424 GGGAC GGGAC(10/14)
855 GTCCC
Bsp1407I 351 TGTACA T^GTACA
1181 TGTACA
BsrGI 351 TGTACA T^GTACA
1181 TGTACA
BstUI 610 CGCG CG^CG
1710 CGCG
DraIII 807 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1138 CACCTGGTG
HaeII 344 GGCGCC [GA]GCGC^[CT]
1676 GGCGCT
HapII 1674 CCGG C^CGG
1770 CCGG
HpaII 1674 CCGG C^CGG
1770 CCGG
MaeII 831 ACGT A^CGT
892 ACGT
MslI 114 CATTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1252 CACAGTGATG
MspI 1674 CCGG C^CGG
1770 CCGG
MvnI 610 CGCG CG^CG
1710 CGCG
NspI 1628 ACATGC [GA]CATG^[CT]
1781 GCATGC
PstI 553 CTGCAG CTGCA^G
902 CTGCAG
SacI 210 GAGCTC GAGCT^C
1201 GAGCTC
ScaI 681 AGTACT AGT^ACT
1113 AGTACT
SfaNI 913 GATGC GCATC(5/9)
1647 GCATC
SfcI 553 CTGCAG C^T[GA][CT]AG
902 CTGCAG
SspBI 351 TGTACA T^GTACA
1181 TGTACA
StuI 125 AGGCCT AGG^CCT
515 AGGCCT
TfiI 748 GATTC G^A[AT]TC
1017 GAATC
Tsp509I 1089 AATT ^AATT
1321 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AfaI AluI ApaI
BalI BanI BanII BbvI BfaI
BmyI BsaHI BseRI BsgI BsiYI
BslI BsmAI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HgaI HhaI
Hin1I HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SmlI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I