(Circular) (Six-base) MAPSORT of: pFN21AB3684.seq Check: 6153 from: 1 to: 6496 pFN21AB3684 6496 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2900 3480 2900 Size: 580 5916 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4306 278 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 3839 1937 Size: 1071 831 4594 Fragments arranged by size: 4594 1071 831 Acc65I G'GTAC_C Cuts at: 3817 3817 Size: 6496 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2112 2669 3552 4559 5943 6211 1632 Size: 480 557 883 1007 1384 268 1917 Fragments arranged by size: 1917 1384 1007 883 557 480 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6299 AflIII A'CryG_T Cuts at: 1203 2836 5395 6372 1203 Size: 1633 2559 977 1327 Fragments arranged by size: 2559 1633 1327 977 AgeI A'CCGG_T Cuts at: 5327 5327 Size: 6496 AhdI GACnn_n'nnGTC Cuts at: 2422 5225 2422 Size: 2803 3693 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6496 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6496 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3143 3175 3143 Size: 32 6464 AlwNI CAG_nnn'CTG Cuts at: 4239 5811 4239 Size: 1572 4924 ApaI G_GGCC'C Cuts at: 2410 3271 3770 2410 Size: 861 499 5136 Fragments arranged by size: 5136 861 499 ApaLI G'TGCA_C Cuts at: 4553 5709 4553 Size: 1156 5340 ApoI r'AATT_y Cuts at: 1493 3805 4031 4183 6292 1493 Size: 2312 226 152 2109 1697 Fragments arranged by size: 2312 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2271 5050 160 Size: 872 1239 2779 1606 Fragments arranged by size: 2779 1606 1239 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 3821 1870 Size: 73 1878 4545 Fragments arranged by size: 4545 1878 73 BamHI G'GATC_C Cuts at: 3826 4193 3826 Size: 367 6129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2349 2618 3520 3817 5272 Size: 325 217 627 562 269 902 297 1455 Cuts at: 5272 618 Size: 1842 Fragments arranged by size: 1842 1455 902 627 562 325 297 269 217 BanII G_rGCy'C Cuts at: 1391 2219 2410 3210 3271 3462 3770 3815 1391 Size: 828 191 800 61 191 308 45 4072 Fragments arranged by size: 4072 828 800 308 191 191 61 45 BbeI G_GCGC'C Cuts at: 2353 2353 Size: 6496 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3237 928 Size: 971 1338 4187 Fragments arranged by size: 4187 1338 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4687 4721 1903 Size: 34 2750 34 3678 Fragments arranged by size: 3678 2750 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4388 5598 4388 Size: 1210 5286 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BfrBI ATG'CAT Cuts at: 3652 3652 Size: 6496 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2528 3198 5107 Size: 107 122 71 631 605 856 670 1909 Cuts at: 5107 136 Size: 1525 Fragments arranged by size: 1909 1525 856 670 631 605 122 107 71 BglII A'GATC_T Cuts at: 3513 6491 3513 Size: 2978 3518 BlpI GC'TnA_GC Cuts at: 3913 3913 Size: 6496 Bme1580I G_kGCm'C Cuts at: 1790 2410 2506 2776 3271 3378 3523 3770 4557 Size: 620 96 270 495 107 145 247 787 Cuts at: 4557 5713 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 787 620 495 270 247 145 107 96 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2166 2428 3265 Size: 416 53 83 399 24 749 262 837 Cuts at: 3265 4227 5185 442 Size: 962 958 1753 Fragments arranged by size: 1753 962 958 837 749 416 399 262 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2106 5156 1239 Size: 867 3050 2579 Fragments arranged by size: 3050 2579 867 Bpu10I CC'TnA_GC Cuts at: 2218 2218 Size: 6496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2591 3107 3476 4621 5486 5784 2591 Size: 516 369 1145 865 298 3303 Fragments arranged by size: 3303 1145 865 516 369 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5159 882 Size: 617 3660 2219 Fragments arranged by size: 3660 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4287 493 Size: 711 3083 2702 Fragments arranged by size: 3083 2702 711 BsaBI GATnn'nnATC Cuts at: 2280 6490 2280 Size: 4210 2286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2350 3321 3670 4303 Size: 53 83 186 936 817 971 349 633 Cuts at: 4303 4685 5320 275 Size: 382 635 1451 Fragments arranged by size: 1451 971 936 817 635 633 382 349 186 83 53 BsaWI w'CCGG_w Cuts at: 4929 5327 5601 5748 6159 4929 Size: 398 274 147 411 5266 Fragments arranged by size: 5266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3429 3459 1178 Size: 30 165 30 2026 30 4215 Fragments arranged by size: 4215 2026 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2330 2342 3241 3730 3773 3776 1186 Size: 904 240 12 899 489 43 3 3906 Fragments arranged by size: 3906 904 899 489 240 43 12 3 BseYI C'CCAG_C Cuts at: 2491 2844 2956 3734 5699 6195 2491 Size: 353 112 778 1965 496 2792 Fragments arranged by size: 2792 1965 778 496 353 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2564 2585 3800 1163 Size: 573 828 21 1215 3859 Fragments arranged by size: 3859 1215 828 573 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3946 4707 5735 1540 Size: 275 186 1945 761 1028 2301 Fragments arranged by size: 2301 1945 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2219 3210 4557 4642 5713 2219 Size: 991 1347 85 1071 3002 Fragments arranged by size: 3002 1347 1071 991 85 BsmI GAATG_Cn' Cuts at: 4007 4100 4007 Size: 93 6403 BsmBI CGTCTCn'nnnn_ Cuts at: 3661 3661 Size: 6496 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2410 2506 2776 3210 3271 3378 Size: 399 429 191 96 270 434 61 107 Cuts at: 3378 3462 3523 3770 3815 4557 4642 5713 1391 Size: 84 61 247 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 434 429 399 270 247 191 107 96 85 84 61 61 45 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 4385 6115 4385 Size: 1730 4766 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2776 2900 3480 3851 844 Size: 1932 124 580 371 3489 Fragments arranged by size: 3489 1932 580 371 124 BsrBI CCG'CTC Cuts at: 1394 1823 2298 2404 2485 3360 3589 4383 1394 Size: 429 475 106 81 875 229 794 3507 Fragments arranged by size: 3507 875 794 475 429 229 106 81 BsrDI GCAATG_nn' Cuts at: 65 1248 4991 5165 65 Size: 1183 3743 174 1396 Fragments arranged by size: 3743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3678 5140 5327 1700 Size: 110 1868 1462 187 2869 Fragments arranged by size: 2869 1868 1462 187 110 BsrGI T'GTAC_A Cuts at: 96 2356 3186 96 Size: 2260 830 3406 Fragments arranged by size: 3406 2260 830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 4556 5568 4556 Size: 1012 5484 BstAPI GCAn_nnn'nTGC Cuts at: 794 3787 794 Size: 2993 3503 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstYI r'GATC_y Cuts at: 1972 3513 3826 4193 4578 4595 6047 6133 6145 Size: 1541 313 367 385 17 1452 86 12 Cuts at: 6145 6491 1972 Size: 346 1977 Fragments arranged by size: 1977 1541 1452 385 367 346 313 86 17 12 BstZ17I GTA'TAC Cuts at: 3009 3009 Size: 6496 Bsu36I CC'TnA_GG Cuts at: 2113 2113 Size: 6496 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5944 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2655 487 Size: 683 56 1429 4328 Fragments arranged by size: 4328 1429 683 56 BtsI GCAGTG_nn' Cuts at: 3054 3788 4012 4806 4826 3054 Size: 734 224 794 20 4724 Fragments arranged by size: 4724 794 734 224 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2820 2855 529 Size: 35 2256 35 4170 Fragments arranged by size: 4170 2256 35 35 DraI TTT'AAA Cuts at: 3800 3800 Size: 6496 DraIII CAC_nnn'GTG Cuts at: 2817 3148 2817 Size: 331 6165 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5503 809 Size: 507 4187 1802 Fragments arranged by size: 4187 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 2704 2763 Size: 54 1425 50 275 18 798 76 59 Cuts at: 2763 3213 3943 4832 6461 8 Size: 450 730 889 1629 43 Fragments arranged by size: 1629 1425 889 798 730 450 275 76 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3943 1537 Size: 275 2131 4090 Fragments arranged by size: 4090 2131 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 2301 2307 2319 2331 3248 4426 1353 Size: 735 213 6 12 12 917 1178 3423 Fragments arranged by size: 3423 1178 917 735 213 12 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2543 5083 5457 5603 387 Size: 1064 138 954 2540 374 146 1280 Fragments arranged by size: 2540 1280 1064 954 374 146 138 EcoICRI GAG'CTC Cuts at: 2217 3208 2217 Size: 991 5505 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2112 2669 3552 4559 5156 5943 Size: 393 474 6 557 883 1007 597 787 Cuts at: 5943 6211 1239 Size: 268 1524 Fragments arranged by size: 1524 1007 883 787 597 557 474 393 268 6 EcoO109I rG'GnC_Cy Cuts at: 2407 3267 3766 3767 2407 Size: 860 499 1 5136 Fragments arranged by size: 5136 860 499 1 EcoRI G'AATT_C Cuts at: 3805 4183 3805 Size: 378 6118 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 2353 3685 5643 1678 Size: 675 1332 1958 2531 Fragments arranged by size: 2531 1958 1332 675 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2219 2251 2372 Size: 32 135 32 494 32 121 32 121 Cuts at: 2372 2404 2408 2440 3383 3415 3658 3690 3704 Size: 32 4 32 943 32 243 32 14 Cuts at: 3704 3736 5137 5169 5211 5243 1373 Size: 32 1401 32 42 32 2626 Fragments arranged by size: 2626 1401 943 494 243 135 121 121 42 32 32 32 32 32 32 32 32 32 32 32 14 4 HincII GTy'rAC Cuts at: 669 1657 1938 3840 4086 669 Size: 988 281 1902 246 3079 Fragments arranged by size: 3079 1902 988 281 246 HindIII A'AGCT_T Cuts at: 748 3534 748 Size: 2786 3710 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KasI G'GCGC_C Cuts at: 2349 2349 Size: 6496 KpnI G_GTAC'C Cuts at: 3821 3821 Size: 6496 MfeI C'AATT_G Cuts at: 4095 4095 Size: 6496 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5610 5794 1305 Size: 4305 184 2007 Fragments arranged by size: 4305 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2630 2706 2765 3215 10 Size: 54 1425 343 798 76 59 450 3291 Fragments arranged by size: 3291 1425 798 450 343 76 59 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 3261 4454 4813 4972 518 Size: 648 957 1138 1193 359 159 2042 Fragments arranged by size: 2042 1193 1138 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3530 3912 4589 5737 5982 1606 Size: 1924 382 677 1148 245 2120 Fragments arranged by size: 2120 1924 1148 677 382 245 NarI GG'CG_CC Cuts at: 2350 2350 Size: 6496 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5944 NdeI CA'TA_TG Cuts at: 387 387 Size: 6496 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NsiI A_TGCA'T Cuts at: 3654 3654 Size: 6496 NspI r_CATG'y Cuts at: 1132 1229 3637 3790 3854 5399 1132 Size: 97 2408 153 64 1545 2229 Fragments arranged by size: 2408 2229 1545 153 97 64 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6496 PciI A'CATG_T Cuts at: 5395 5395 Size: 6496 PflMI CCAn_nnn'nTGG Cuts at: 1108 2869 1108 Size: 1761 4735 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4565 4597 6078 6110 4565 Size: 32 1481 32 4951 Fragments arranged by size: 4951 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2256 1613 Size: 643 5853 PsiI TTA'TAA Cuts at: 4066 4066 Size: 6496 PspOMI G'GGCC_C Cuts at: 2406 3267 3766 2406 Size: 861 499 5136 Fragments arranged by size: 5136 861 499 PstI C_TGCA'G Cuts at: 830 2562 2911 3848 830 Size: 1732 349 937 3478 Fragments arranged by size: 3478 1732 937 349 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 3530 3530 Size: 6496 SacI G_AGCT'C Cuts at: 2219 3210 2219 Size: 991 5505 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SapI GCTCTTCn'nnn_ Cuts at: 2331 2331 Size: 6496 SbfI CC_TGCA'GG Cuts at: 3848 3848 Size: 6496 ScaI AGT'ACT Cuts at: 2688 3120 4744 6456 2688 Size: 432 1624 1712 2728 Fragments arranged by size: 2728 1712 1624 432 SexAI A'CCwGG_T Cuts at: 3144 3144 Size: 6496 SfcI C'TryA_G Cuts at: 826 1045 2558 2907 3844 4979 5660 5851 826 Size: 219 1513 349 937 1135 681 191 1471 Fragments arranged by size: 1513 1471 1135 937 681 349 219 191 SfoI GGC'GCC Cuts at: 2351 2351 Size: 6496 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 3678 1700 Size: 1978 4518 SmaI CCC'GGG Cuts at: 1872 3823 1872 Size: 1951 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2570 3122 3455 4600 5501 5763 Size: 197 926 627 552 333 1145 901 262 Cuts at: 5763 820 Size: 1553 Fragments arranged by size: 1553 1145 926 901 627 552 333 262 197 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6496 SphI G_CATG'C Cuts at: 1132 3790 3854 1132 Size: 2658 64 3774 Fragments arranged by size: 3774 2658 64 SspI AAT'ATT Cuts at: 5 52 4420 5 Size: 47 4368 2081 Fragments arranged by size: 4368 2081 47 StuI AGG'CCT Cuts at: 1517 2132 2522 1517 Size: 615 390 5491 Fragments arranged by size: 5491 615 390 StyI C'CwwG_G Cuts at: 513 1065 3935 513 Size: 552 2870 3074 Fragments arranged by size: 3074 2870 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2410 3041 3538 4523 4540 4693 4878 Size: 1255 830 631 497 985 17 153 185 Cuts at: 4878 5323 325 Size: 445 1498 Fragments arranged by size: 1498 1255 985 830 631 497 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2356 2686 3118 Size: 274 80 33 51 1443 379 330 432 Cuts at: 3118 3186 4742 6454 96 Size: 68 1556 1712 138 Fragments arranged by size: 1712 1556 1443 432 379 330 274 138 80 68 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6456 6488 6456 Size: 32 6464 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 3821 1870 Size: 1951 4545 XmnI GAAnn'nnTTC Cuts at: 4625 4625 Size: 6496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4304 276 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BsiWI BstXI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI