GJA10 ORF clone FHC05295

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC05295

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Clone info.

Species

human

Clone Name

pFN21AE0353 Sequence (with vector) Restriction Map

Product ID

FHC05295

Flexi Clone PID

FXC05295

ORF Sequence

DNA sequence (1629 bp)
Protein sequence (543 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527151]

Gene

KIEE0353

Symbol

GJA10

Alias : CX62

Description

gap junction protein, alpha 10, 62kDa

Original Clone ID

oc01053

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1629 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 543 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5025.1 7.6e-211 100.0 GJA10 CCDS432.1 1.8e-63 47.7 GJA9 CCDS30834.1 9.8e-49 38.2 GJA8 CCDS9289.1 9.9e-49 52.9 GJA3 CCDS929.1 1.4e-46 44.4 GJA5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000500 21 45 PR00206 Connexin IPR000500 52 74 PR00206 Connexin IPR000500 77 97 PR00206 Connexin IPR000500 153 179 PR00206 Connexin IPR000500 189 209 PR00206 Connexin IPR000500 210 233 PR00206 Connexin HMMPfam IPR013092 3 108 PF00029 Connexin IPR019570 167 233 PF10582 Gap junction protein HMMSmart IPR013092 43 76 SM00037 Connexin
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_115991 543 611924 100.0 100.0 XP_011507719 427 116200,600897,612474 38.4 80.1 XP_011507718 433 116200,600897,612474 38.2 80.8 NP_005258 433 116200,600897,612474 38.2 80.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0353 Download

>KIEE0353  1629 bp
ATGGGGGACTGGAACTTATTGGGTGGCATCCTAGAGGAAGTTCACTCCCACTCAACCATA
GTGGGGAAAATCTGGCTGACCATCCTCTTCATCTTCCGAATGCTGGTACTTCGTGTGGCT
GCTGAGGATGTCTGGGATGATGAACAGTCAGCATTTGCCTGCAACACCCGGCAGCCAGGT
TGCAACAATATCTGTTATGATGATGCATTCCCTATCTCTTTGATCAGGTTCTGGGTTTTA
CAGATCATCTTTGTGTCTTCTCCTTCTTTGGTCTATATGGGCCATGCACTTTATAGGCTC
AGGGCCTTTGAGAAAGACAGGCAGAGGAAAAAGTCACACCTTAGAGCCCAGATGGAGAAT
CCAGATCTTGACTTGGAGGAGCAGCAAAGAATAGATAGGGAACTGAGGAGGTTAGAGGAG
CAGAAGAGGATCCATAAAGTCCCTCTGAAAGGATGTCTGCTGCGTACTTATGTCTTACAC
ATCTTGACCAGATCTGTGCTGGAAGTAGGATTCATGATAGGCCAATATATTCTCTATGGG
TTTCAAATGCACCCCCTTTACAAATGCACTCAACCTCCTTGCCCCAATGCGGTGGATTGC
TTTGTATCCAGGCCCACTGAGAAGACAATTTTCATGCTTTTTATGCACAGCATTGCAGCC
ATTTCCTTGTTACTCAATATACTGGAAATATTTCATCTAGGCATCAGAAAAATTATGAGG
ACACTTTATAAGAAATCCAGCAGTGAGGGCATTGAGGATGAAACAGGCCCTCCATTCCAT
TTGAAGAAATATTCTGTGGCCCAGCAGTGTATGATTTGCTCTTCATTGCCTGAAAGAATC
TCTCCACTTCAAGCTAACAATCAACAGCAAGTCATTCGAGTTAATGTGCCAAAGTCTAAA
ACCATGTGGCAAATCCCACAGCCAAGGCAACTTGAAGTAGACCCTTCCAATGGGAAAAAG
GACTGGTCTGAGAAGGATCAGCATAGCGGACAGCTCCATGTTCACAGCCCGTGTCCCTGG
GCTGGCAGTGCTGGAAATCAGCACCTGGGACAGCAATCAGACCATTCCTCATTTGGCCTG
CAGAATACAATGTCTCAGTCCTGGCTAGGTACAACTACGGCTCCTAGAAACTGTCCATCC
TTTGCAGTAGGAACCTGGGAGCAGTCCCAGGACCCAGAACCCTCAGGTGAGCCTCTCACA
GATCTTCATAGTCACTGCAGAGACAGTGAAGGCAGCATGAGAGAGAGTGGGGTCTGGATA
GACAGATCTCGCCCAGGCAGTCGCAAGGCCAGCTTTCTGTCCAGATTGTTGTCTGAAAAG
CGACATCTGCACAGTGACTCAGGAAGCTCTGGTTCTCGGAATAGCTCCTGCTTGGATTTT
CCTCACTGGGAAAACAGCCCCTCACCTCTGCCTTCAGTCACTGGGCACAGAACATCAATG
GTAAGACAGGCAGCCCTACCGATCATGGAACTATCACAAGAGCTGTTCCATTCTGGATGC
TTTCTTTTTCCTTTCTTTCTTCCTGGGGTGTGTATGTATGTTTGTGTTGACAGAGAGGCA
GATGGAGGGGGAGATTATTTATGGAGAGATAAAATTATTCATTCGATACATTCAGTTAAA
TTCAATTCA

ORF Protein

Cloned ORF protein sequence for pFN21AE0353 Download

>KIEE0353  543 aa
MGDWNLLGGILEEVHSHSTIVGKIWLTILFIFRMLVLRVAAEDVWDDEQSAFACNTRQPG
CNNICYDDAFPISLIRFWVLQIIFVSSPSLVYMGHALYRLRAFEKDRQRKKSHLRAQMEN
PDLDLEEQQRIDRELRRLEEQKRIHKVPLKGCLLRTYVLHILTRSVLEVGFMIGQYILYG
FQMHPLYKCTQPPCPNAVDCFVSRPTEKTIFMLFMHSIAAISLLLNILEIFHLGIRKIMR
TLYKKSSSEGIEDETGPPFHLKKYSVAQQCMICSSLPERISPLQANNQQQVIRVNVPKSK
TMWQIPQPRQLEVDPSNGKKDWSEKDQHSGQLHVHSPCPWAGSAGNQHLGQQSDHSSFGL
QNTMSQSWLGTTTAPRNCPSFAVGTWEQSQDPEPSGEPLTDLHSHCRDSEGSMRESGVWI
DRSRPGSRKASFLSRLLSEKRHLHSDSGSSGSRNSSCLDFPHWENSPSPLPSVTGHRTSM
VRQAALPIMELSQELFHSGCFLFPFFLPGVCMYVCVDREADGGGDYLWRDKIIHSIHSVK
FNS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0353 Download

>pFN21AE0353  6334 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGGACTGGAACTTATTGGGTGGCATCCTAGA
GGAAGTTCACTCCCACTCAACCATAGTGGGGAAAATCTGGCTGACCATCCTCTTCATCTT
CCGAATGCTGGTACTTCGTGTGGCTGCTGAGGATGTCTGGGATGATGAACAGTCAGCATT
TGCCTGCAACACCCGGCAGCCAGGTTGCAACAATATCTGTTATGATGATGCATTCCCTAT
CTCTTTGATCAGGTTCTGGGTTTTACAGATCATCTTTGTGTCTTCTCCTTCTTTGGTCTA
TATGGGCCATGCACTTTATAGGCTCAGGGCCTTTGAGAAAGACAGGCAGAGGAAAAAGTC
ACACCTTAGAGCCCAGATGGAGAATCCAGATCTTGACTTGGAGGAGCAGCAAAGAATAGA
TAGGGAACTGAGGAGGTTAGAGGAGCAGAAGAGGATCCATAAAGTCCCTCTGAAAGGATG
TCTGCTGCGTACTTATGTCTTACACATCTTGACCAGATCTGTGCTGGAAGTAGGATTCAT
GATAGGCCAATATATTCTCTATGGGTTTCAAATGCACCCCCTTTACAAATGCACTCAACC
TCCTTGCCCCAATGCGGTGGATTGCTTTGTATCCAGGCCCACTGAGAAGACAATTTTCAT
GCTTTTTATGCACAGCATTGCAGCCATTTCCTTGTTACTCAATATACTGGAAATATTTCA
TCTAGGCATCAGAAAAATTATGAGGACACTTTATAAGAAATCCAGCAGTGAGGGCATTGA
GGATGAAACAGGCCCTCCATTCCATTTGAAGAAATATTCTGTGGCCCAGCAGTGTATGAT
TTGCTCTTCATTGCCTGAAAGAATCTCTCCACTTCAAGCTAACAATCAACAGCAAGTCAT
TCGAGTTAATGTGCCAAAGTCTAAAACCATGTGGCAAATCCCACAGCCAAGGCAACTTGA
AGTAGACCCTTCCAATGGGAAAAAGGACTGGTCTGAGAAGGATCAGCATAGCGGACAGCT
CCATGTTCACAGCCCGTGTCCCTGGGCTGGCAGTGCTGGAAATCAGCACCTGGGACAGCA
ATCAGACCATTCCTCATTTGGCCTGCAGAATACAATGTCTCAGTCCTGGCTAGGTACAAC
TACGGCTCCTAGAAACTGTCCATCCTTTGCAGTAGGAACCTGGGAGCAGTCCCAGGACCC
AGAACCCTCAGGTGAGCCTCTCACAGATCTTCATAGTCACTGCAGAGACAGTGAAGGCAG
CATGAGAGAGAGTGGGGTCTGGATAGACAGATCTCGCCCAGGCAGTCGCAAGGCCAGCTT
TCTGTCCAGATTGTTGTCTGAAAAGCGACATCTGCACAGTGACTCAGGAAGCTCTGGTTC
TCGGAATAGCTCCTGCTTGGATTTTCCTCACTGGGAAAACAGCCCCTCACCTCTGCCTTC
AGTCACTGGGCACAGAACATCAATGGTAAGACAGGCAGCCCTACCGATCATGGAACTATC
ACAAGAGCTGTTCCATTCTGGATGCTTTCTTTTTCCTTTCTTTCTTCCTGGGGTGTGTAT
GTATGTTTGTGTTGACAGAGAGGCAGATGGAGGGGGAGATTATTTATGGAGAGATAAAAT
TATTCATTCGATACATTCAGTTAAATTCAATTCAGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT