(Circular) (Six-base) MAPSORT of: pFN21AE0353.seq Check: 7630 from: 1 to: 6334 pFN21AE0353 6334 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2943 3677 1937 Size: 1006 734 4594 Fragments arranged by size: 4594 1006 734 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3402 4397 5781 6049 1632 Size: 1770 995 1384 268 1917 Fragments arranged by size: 1917 1770 1384 995 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2179 2673 1184 Size: 995 494 4845 Fragments arranged by size: 4845 995 494 AlwNI CAG_nnn'CTG Cuts at: 2235 3049 3425 4077 5649 2235 Size: 814 376 652 1572 2920 Fragments arranged by size: 2920 1572 814 652 376 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 3623 3643 3869 4021 6130 1493 Size: 2130 20 226 152 2109 1697 Fragments arranged by size: 2130 2109 1697 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 3659 1870 Size: 73 1716 4545 Fragments arranged by size: 4545 1716 73 BamHI G'GATC_C Cuts at: 2433 3664 4031 2433 Size: 1231 367 4736 Fragments arranged by size: 4736 1231 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3655 5110 618 Size: 325 217 627 1868 1455 1842 Fragments arranged by size: 1868 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2353 3653 1391 Size: 962 1300 4072 Fragments arranged by size: 4072 1300 962 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 2633 928 Size: 971 354 380 4629 Fragments arranged by size: 4629 971 380 354 BbvCI CC'TCA_GC Cuts at: 2127 2127 Size: 6334 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2861 2895 4525 4559 1903 Size: 34 924 34 1630 34 3678 Fragments arranged by size: 3678 1630 924 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2620 4226 5436 2620 Size: 1606 1210 3518 Fragments arranged by size: 3518 1606 1210 BclI T'GATC_A Cuts at: 1548 2226 1548 Size: 678 5656 BfrBI ATG'CAT Cuts at: 2210 2210 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4945 136 Size: 107 122 71 631 605 3273 1525 Fragments arranged by size: 3273 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2368 2495 3205 3269 6329 2368 Size: 127 710 64 3060 2373 Fragments arranged by size: 3060 2373 710 127 64 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 3432 4395 5551 1790 Size: 1642 963 1156 2573 Fragments arranged by size: 2573 1642 1156 963 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3400 3435 4065 Size: 416 53 83 399 24 1983 35 630 Cuts at: 4065 5023 442 Size: 958 1753 Fragments arranged by size: 1983 1753 958 630 416 399 83 53 35 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4994 1239 Size: 3755 2579 Bpu10I CC'TnA_GC Cuts at: 2127 2303 2127 Size: 176 6158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4459 5324 5622 4459 Size: 865 298 5171 Fragments arranged by size: 5171 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4997 882 Size: 617 3498 2219 Fragments arranged by size: 3498 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4125 493 Size: 711 2921 2702 Fragments arranged by size: 2921 2702 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4141 4523 5158 275 Size: 53 83 186 936 2608 382 635 1451 Fragments arranged by size: 2608 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2563 2593 2758 2788 3109 Size: 30 165 30 1160 30 165 30 321 Cuts at: 3109 3139 1178 Size: 30 4373 Fragments arranged by size: 4373 1160 321 165 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 2425 2435 1186 Size: 1210 29 10 5085 Fragments arranged by size: 5085 1210 29 10 BseYI C'CCAG_C Cuts at: 2805 5537 6033 2805 Size: 2732 496 3106 Fragments arranged by size: 3106 2732 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3317 1163 Size: 573 1581 4180 Fragments arranged by size: 4180 1581 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4395 4480 5551 4395 Size: 85 1071 5178 Fragments arranged by size: 5178 1071 85 BsmI GAATG_Cn' Cuts at: 2109 2210 3845 3938 2109 Size: 101 1635 93 4505 Fragments arranged by size: 4505 1635 101 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2353 3432 3653 4395 4480 5551 1391 Size: 399 563 1079 221 742 85 1071 2174 Fragments arranged by size: 2174 1079 1071 742 563 399 221 85 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 2517 4223 5953 2517 Size: 1706 1730 2898 Fragments arranged by size: 2898 1730 1706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3689 844 Size: 2845 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4221 1394 Size: 429 2398 3507 Fragments arranged by size: 3507 2398 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2655 2828 4829 5003 65 Size: 1183 1407 173 2001 174 1396 Fragments arranged by size: 2001 1407 1396 1183 174 173 BsrFI r'CCGG_y Cuts at: 1700 1810 4978 5165 1700 Size: 110 3168 187 2869 Fragments arranged by size: 3168 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 2596 2596 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2368 2433 2495 3205 3269 3664 4031 4416 Size: 396 65 62 710 64 395 367 385 Cuts at: 4416 4433 5885 5971 5983 6329 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 710 396 395 385 367 346 86 65 64 62 17 12 Bsu36I CC'TnA_GG Cuts at: 3187 3187 Size: 6334 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4843 Fragments arranged by size: 4843 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 2752 2816 3037 3217 3850 4644 4664 2752 Size: 64 221 180 633 794 20 4422 Fragments arranged by size: 4422 794 633 221 180 64 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2046 2081 529 Size: 35 1482 35 4782 Fragments arranged by size: 4782 1482 35 35 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3781 4670 6299 Size: 54 1425 50 275 18 1951 889 1629 Cuts at: 6299 8 Size: 43 Fragments arranged by size: 1951 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2423 2830 4264 1353 Size: 743 327 407 1434 3423 Fragments arranged by size: 3423 1434 743 407 327 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4921 5295 5441 387 Size: 1064 138 3332 374 146 1280 Fragments arranged by size: 3332 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3402 4397 4994 5781 6049 1239 Size: 393 1770 995 597 787 268 1524 Fragments arranged by size: 1770 1524 995 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2451 2451 Size: 6334 EcoO109I rG'GnC_Cy Cuts at: 2307 2771 3175 2307 Size: 464 404 5466 Fragments arranged by size: 5466 464 404 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3313 3313 Size: 6334 FspI TGC'GCA Cuts at: 4840 4840 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 5481 1678 Size: 3803 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2347 2379 3056 Size: 32 135 32 563 32 180 32 677 Cuts at: 3056 3088 4975 5007 5049 5081 1373 Size: 32 1887 32 42 32 2626 Fragments arranged by size: 2626 1887 677 563 180 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3553 3678 3924 669 Size: 988 281 1615 125 246 3079 Fragments arranged by size: 3079 1615 988 281 246 125 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5448 5632 1305 Size: 4143 184 2007 Fragments arranged by size: 4143 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4512 Fragments arranged by size: 4512 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 4292 4651 4810 518 Size: 648 1089 2037 359 159 2042 Fragments arranged by size: 2042 2037 1089 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3750 4427 5575 5820 1606 Size: 2144 677 1148 245 2120 Fragments arranged by size: 2144 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4843 Fragments arranged by size: 4843 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NsiI A_TGCA'T Cuts at: 2212 2212 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 3692 5237 1132 Size: 97 2463 1545 2229 Fragments arranged by size: 2463 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3021 1348 Size: 1673 4661 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3175 3175 Size: 6334 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 2733 3904 2733 Size: 1171 5163 PstI C_TGCA'G Cuts at: 830 3087 3224 3686 830 Size: 2257 137 462 3478 Fragments arranged by size: 3478 2257 462 137 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SapI GCTCTTCn'nnn_ Cuts at: 2830 2830 Size: 6334 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SfcI C'TryA_G Cuts at: 826 1045 3083 3220 3682 4817 5498 5689 826 Size: 219 2038 137 462 1135 681 191 1471 Fragments arranged by size: 2038 1471 1135 681 462 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3661 1872 Size: 1789 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4438 5339 5601 820 Size: 197 926 2495 901 262 1553 Fragments arranged by size: 2495 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SspI AAT'ATT Cuts at: 5 52 2694 2795 4258 5 Size: 47 2642 101 1463 2081 Fragments arranged by size: 2642 2081 1463 101 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6334 StyI C'CwwG_G Cuts at: 513 1065 2004 2927 3773 513 Size: 552 939 923 846 3074 Fragments arranged by size: 3074 939 923 846 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2015 4361 4378 4531 4716 5161 325 Size: 1255 435 2346 17 153 185 445 1498 Fragments arranged by size: 2346 1498 1255 445 435 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4580 6292 96 Size: 274 80 33 51 1443 2603 1712 138 Fragments arranged by size: 2603 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2563 2595 6294 6326 2563 Size: 32 3699 32 2571 Fragments arranged by size: 3699 2571 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2500 3074 2500 Size: 574 5760 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6334 XmaI C'CCGG_G Cuts at: 1870 3659 1870 Size: 1789 4545 XmnI GAAnn'nnTTC Cuts at: 2694 4463 2694 Size: 1769 4565 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI