(Linear) (Six-base) MAPSORT of: KIEE0353.seq  Check: 7269  from: 1  to: 1629



KIEE0353.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0     938    1629 
   Size:        938     691
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1397    1629 
   Size:       1397     232
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     174     668    1629 
   Size:        174     494     961

  Fragments arranged by size: 

                 961     494     174
 
AlwNI CAG_nnn'CTG

Cuts at:      0     230    1044    1420    1629 
   Size:        230     814     376     209

  Fragments arranged by size: 

                 814     376     230     209
 
ApoI r'AATT_y

Cuts at:      0    1618    1629 
   Size:       1618      11
 
BamHI G'GATC_C

Cuts at:      0     428    1629 
   Size:        428    1201
 
BanII G_rGCy'C

Cuts at:      0     348    1629 
   Size:        348    1281
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     248     628    1629 
   Size:        248     380    1001

  Fragments arranged by size: 

                1001     380     248
 
BbvCI CC'TCA_GC

Cuts at:      0     122    1629 
   Size:        122    1507
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     856     890    1629 
   Size:        856      34     739

  Fragments arranged by size: 

                 856     739      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     615    1629 
   Size:        615    1014
 
BclI T'GATC_A

Cuts at:      0     221    1629 
   Size:        221    1408
 
BfrBI ATG'CAT

Cuts at:      0     205    1629 
   Size:        205    1424
 
BglII A'GATC_T

Cuts at:      0     363     490    1200    1264    1629 
   Size:        363     127     710      64     365

  Fragments arranged by size: 

                 710     365     363     127      64
 
Bme1580I G_kGCm'C

Cuts at:      0    1427    1629 
   Size:       1427     202
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1395    1430    1629 
   Size:       1395      35     199

  Fragments arranged by size: 

                1395     199      35
 
Bpu10I CC'TnA_GC

Cuts at:      0     122     298    1629 
   Size:        122     176    1331

  Fragments arranged by size: 

                1331     176     122
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     558     588     753     783    1104    1134    1629 
   Size:        558      30     165      30     321      30     495

  Fragments arranged by size: 

                 558     495     321     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     391     420     430    1629 
   Size:        391      29      10    1199

  Fragments arranged by size: 

                1199     391      29      10
 
BseYI C'CCAG_C

Cuts at:      0     800    1629 
   Size:        800     829
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1312    1629 
   Size:       1312     317
 
BsmI GAATG_Cn'

Cuts at:      0     104     205    1629 
   Size:        104     101    1424

  Fragments arranged by size: 

                1424     104     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     348    1427    1629 
   Size:        348    1079     202

  Fragments arranged by size: 

                1079     348     202
 
BspHI T'CATG_A

Cuts at:      0     512    1629 
   Size:        512    1117
 
BsrDI GCAATG_nn'

Cuts at:      0     650     823    1629 
   Size:        650     173     806

  Fragments arranged by size: 

                 806     650     173
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     591    1629 
   Size:        591    1038
 
BstYI r'GATC_y

Cuts at:      0     363     428     490    1200    1264    1629 
   Size:        363      65      62     710      64     365

  Fragments arranged by size: 

                 710     365     363      65      64      62
 
Bsu36I CC'TnA_GG

Cuts at:      0    1182    1629 
   Size:       1182     447
 
BtsI GCAGTG_nn'

Cuts at:      0     747     811    1032    1212    1629 
   Size:        747      64     221     180     417

  Fragments arranged by size: 

                 747     417     221     180      64
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      41      76    1629 
   Size:         41      35    1553

  Fragments arranged by size: 

                1553      41      35
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     418     825    1629 
   Size:         91     327     407     804

  Fragments arranged by size: 

                 804     407     327      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1397    1629 
   Size:       1397     232
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     446    1629 
   Size:        446    1183
 
EcoO109I rG'GnC_Cy

Cuts at:      0     302     766    1170    1629 
   Size:        302     464     404     459

  Fragments arranged by size: 

                 464     459     404     302
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1308    1629 
   Size:       1308     321
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     342     374    1051    1083    1629 
   Size:        130      32     180      32     677      32     546

  Fragments arranged by size: 

                 677     546     180     130      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1548    1629 
   Size:       1548      81
 
MslI CAynn'nnrTG

Cuts at:      0     250    1629 
   Size:        250    1379
 
NsiI A_TGCA'T

Cuts at:      0     207    1629 
   Size:        207    1422
 
PasI CC'CwG_GG

Cuts at:      0    1016    1629 
   Size:       1016     613
 
PpuMI rG'GwC_Cy

Cuts at:      0    1170    1629 
   Size:       1170     459
 
PsiI TTA'TAA

Cuts at:      0     728    1629 
   Size:        728     901
 
PstI C_TGCA'G

Cuts at:      0    1082    1219    1629 
   Size:       1082     137     410

  Fragments arranged by size: 

                1082     410     137
 
SapI GCTCTTCn'nnn_

Cuts at:      0     825    1629 
   Size:        825     804
 
SfcI C'TryA_G

Cuts at:      0    1078    1215    1629 
   Size:       1078     137     414

  Fragments arranged by size: 

                1078     414     137
 
SspI AAT'ATT

Cuts at:      0     689     790    1629 
   Size:        689     101     839

  Fragments arranged by size: 

                 839     689     101
 
StyI C'CwwG_G

Cuts at:      0     922    1629 
   Size:        922     707
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      10    1629 
   Size:         10    1619
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     558     590    1629 
   Size:        558      32    1039

  Fragments arranged by size: 

                1039     558      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     495    1069    1629 
   Size:        495     574     560

  Fragments arranged by size: 

                 574     560     495
 
XmnI GAAnn'nnTTC

Cuts at:      0     689    1629 
   Size:        689     940
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI    BamHI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I     BmrI
   Bpu10I    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspHI
    BsrDI    BstXI    BstYI   Bsu36I     BtsI    CspCI     EarI  Eco57MI
    EcoNI EcoO109I     FalI    Hin4I   HincII     MslI     NsiI     PasI
    PpuMI     PsiI     PstI     SapI     SfcI     SspI     StyI    TaqII
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BglI     BlpI    BmgBI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
     ZraI