SPAG4 ORF clone FHC02306

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Halotag ORF Clone FHC02306

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Clone info.

Species

human

Clone Name

pFN21AB7186 Sequence (with vector) Restriction Map

Product ID

FHC02306

Flexi Clone PID

FXC02306

ORF Sequence

DNA sequence (1311 bp)
Protein sequence (437 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464242]

Gene

KIBB7186

Symbol

SPAG4

Alias : CT127, SUN4

Description

sperm associated antigen 4

Original Clone ID

oc00490

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1311 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 437 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13259.1 2.3e-144 100.0 SPAG4 CCDS13209.1 2.3e-33 37.1 SUN5 CCDS13978.1 1.3e-21 39.7 SUN2 CCDS56231.1 1.4e-21 39.7 SUN2 CCDS64647.1 2.4e-21 36.4 SUN3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR012919 292 423 PF07738 Sad1/UNC-like ProfileScan IPR012919 265 425 PS51469 Sad1/UNC-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 134 SFLSLLFQGLSVLLSLAGDVLVS 156 SECONDARY 23 2 166 FLFTAVSLLSLFLSAFWLGLLYL 188 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003107 437 603038 100.0 100.0 XP_011527311 393 603038 89.9 100.0 NP_542406 379 613942 37.1 85.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7186 Download

>KIBB7186  1311 bp
ATGCGGCGAAGCTCCCGCCCGGGCTCGGCCTCGTCCTCGCGCAAGCACACGCCCAACTTT
TTCAGCGAGAACAGCTCAATGAGCATCACCTCGGAGGACAGCAAAGGGCTCCGGTCAGCG
GAGCCCGGGCCTGGGGAGCCCGAGGGCAGAAGAGCCCGGGGCCCGAGCTGCGGTGAGCCC
GCCTTGAGCGCGGGAGTGCCCGGAGGAACCACATGGGCAGGAAGCTCTCAGCAGAAGCCA
GCGCCTCGGAGCCACAACTGGCAGACAGCCTGTGGCGCGGCAACCGTGAGGGGCGGGGCC
TCGGAACCGACTGGGTCTCCAGTAGTCTCTGAGGAGCCGCTCGACCTTCTCCCGACCCTG
GATCTGAGGCAGGAGATGCCTCCCCCGCGGGTGTTCAAGAGCTTTCTGAGCCTGCTCTTC
CAGGGGCTGAGCGTGTTGTTATCCCTGGCAGGAGACGTGCTGGTCAGCATGTACAGGGAG
GTCTGTTCCATCCGCTTCCTGTTCACGGCTGTGTCGCTGCTGAGCCTCTTTCTGTCAGCA
TTCTGGCTGGGGCTTCTGTACCTGGTCTCTCCTTTGGAGAATGAACCTAAGGAGATGCTG
ACTCTAAGTGAGTACCACGAGCGCGTGCGCTCCCAGGGGCAGCAGCTGCAGCAGCTCCAG
GCCGAGCTGGATAAACTCCACAAGGAGGTGTCCACTGTTCGGGCAGCCAACAGCGAGAGA
GTGGCCAAGCTCGTGTTCCAGAGGCTGAATGAGGATTTTGTGCGGAAGCCCGACTATGCT
TTGAGCTCTGTGGGAGCCTCCATCGACCTGCAGAAGACATCCCACGATTACGCAGACAGG
AACACTGCCTACTTCTGGAATCGCTTCAGCTTCTGGAACTACGCACGGCCGCCCACGGTT
ATCCTGGAGCCCCACGTGTTCCCTGGGAATTGCTGGGCTTTTGAAGGCGACCAAGGCCAG
GTGGTGATCCAACTGCCGGGCCGAGTGCAGCTGAGCGACATCACTCTGCAGCATCCACCG
CCCAGCGTGGAGCACACCGGAGGAGCCAACAGCGCCCCCCGCGATTTCGCGGTCTTTGGC
CTCCAGGTTTATGATGAAACTGAAGTTTCCTTGGGGAAATTCACCTTCGATGTTGAGAAA
TCGGAGATTCAGACTTTCCACCTGCAGAATGACCCCCCAGCTGCCTTTCCCAAGGTGAAG
ATCCAGATTCTAAGCAACTGGGGCCACCCCCGTTTCACGTGCTTGTATCGAGTCCGTGCC
CACGGTGTGCGAACCTCAGAGGGGGCAGAGGGCAGTGCACAGGGGCCCCAT

ORF Protein

Cloned ORF protein sequence for pFN21AB7186 Download

>KIBB7186  437 aa
MRRSSRPGSASSSRKHTPNFFSENSSMSITSEDSKGLRSAEPGPGEPEGRRARGPSCGEP
ALSAGVPGGTTWAGSSQQKPAPRSHNWQTACGAATVRGGASEPTGSPVVSEEPLDLLPTL
DLRQEMPPPRVFKSFLSLLFQGLSVLLSLAGDVLVSMYREVCSIRFLFTAVSLLSLFLSA
FWLGLLYLVSPLENEPKEMLTLSEYHERVRSQGQQLQQLQAELDKLHKEVSTVRAANSER
VAKLVFQRLNEDFVRKPDYALSSVGASIDLQKTSHDYADRNTAYFWNRFSFWNYARPPTV
ILEPHVFPGNCWAFEGDQGQVVIQLPGRVQLSDITLQHPPPSVEHTGGANSAPRDFAVFG
LQVYDETEVSLGKFTFDVEKSEIQTFHLQNDPPAAFPKVKIQILSNWGHPRFTCLYRVRA
HGVRTSEGAEGSAQGPH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7186 Download

>pFN21AB7186  6016 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCGGCGAAGCTCCCGCCCGGGCTCGGCCTCGTC
CTCGCGCAAGCACACGCCCAACTTTTTCAGCGAGAACAGCTCAATGAGCATCACCTCGGA
GGACAGCAAAGGGCTCCGGTCAGCGGAGCCCGGGCCTGGGGAGCCCGAGGGCAGAAGAGC
CCGGGGCCCGAGCTGCGGTGAGCCCGCCTTGAGCGCGGGAGTGCCCGGAGGAACCACATG
GGCAGGAAGCTCTCAGCAGAAGCCAGCGCCTCGGAGCCACAACTGGCAGACAGCCTGTGG
CGCGGCAACCGTGAGGGGCGGGGCCTCGGAACCGACTGGGTCTCCAGTAGTCTCTGAGGA
GCCGCTCGACCTTCTCCCGACCCTGGATCTGAGGCAGGAGATGCCTCCCCCGCGGGTGTT
CAAGAGCTTTCTGAGCCTGCTCTTCCAGGGGCTGAGCGTGTTGTTATCCCTGGCAGGAGA
CGTGCTGGTCAGCATGTACAGGGAGGTCTGTTCCATCCGCTTCCTGTTCACGGCTGTGTC
GCTGCTGAGCCTCTTTCTGTCAGCATTCTGGCTGGGGCTTCTGTACCTGGTCTCTCCTTT
GGAGAATGAACCTAAGGAGATGCTGACTCTAAGTGAGTACCACGAGCGCGTGCGCTCCCA
GGGGCAGCAGCTGCAGCAGCTCCAGGCCGAGCTGGATAAACTCCACAAGGAGGTGTCCAC
TGTTCGGGCAGCCAACAGCGAGAGAGTGGCCAAGCTCGTGTTCCAGAGGCTGAATGAGGA
TTTTGTGCGGAAGCCCGACTATGCTTTGAGCTCTGTGGGAGCCTCCATCGACCTGCAGAA
GACATCCCACGATTACGCAGACAGGAACACTGCCTACTTCTGGAATCGCTTCAGCTTCTG
GAACTACGCACGGCCGCCCACGGTTATCCTGGAGCCCCACGTGTTCCCTGGGAATTGCTG
GGCTTTTGAAGGCGACCAAGGCCAGGTGGTGATCCAACTGCCGGGCCGAGTGCAGCTGAG
CGACATCACTCTGCAGCATCCACCGCCCAGCGTGGAGCACACCGGAGGAGCCAACAGCGC
CCCCCGCGATTTCGCGGTCTTTGGCCTCCAGGTTTATGATGAAACTGAAGTTTCCTTGGG
GAAATTCACCTTCGATGTTGAGAAATCGGAGATTCAGACTTTCCACCTGCAGAATGACCC
CCCAGCTGCCTTTCCCAAGGTGAAGATCCAGATTCTAAGCAACTGGGGCCACCCCCGTTT
CACGTGCTTGTATCGAGTCCGTGCCCACGGTGTGCGAACCTCAGAGGGGGCAGAGGGCAG
TGCACAGGGGCCCCATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT