(Circular) (Six-base) MAPSORT of: pFN21AB7186.seq Check: 5909 from: 1 to: 6016 pFN21AB7186 6016 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3174 3174 Size: 6016 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3359 1937 Size: 1422 4594 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2854 3126 4079 5463 5731 1632 Size: 1222 272 953 1384 268 1917 Fragments arranged by size: 1917 1384 1222 953 272 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 2919 4915 5892 1203 Size: 1716 1996 977 1327 Fragments arranged by size: 1996 1716 1327 977 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 2513 1166 Size: 1347 4669 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6016 AlwNI CAG_nnn'CTG Cuts at: 2431 2650 2749 2877 3759 5331 2431 Size: 219 99 128 882 1572 3116 Fragments arranged by size: 3116 1572 882 219 128 99 ApaI G_GGCC'C Cuts at: 2168 3312 2168 Size: 1144 4872 ApaLI G'TGCA_C Cuts at: 3300 4073 5229 3300 Size: 773 1156 4087 Fragments arranged by size: 4087 1156 773 ApoI r'AATT_y Cuts at: 1493 3122 3325 3551 3703 5812 1493 Size: 1629 203 226 152 2109 1697 Fragments arranged by size: 2109 1697 1629 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 2023 2129 2144 2160 2167 3341 1870 Size: 73 80 106 15 16 7 1174 4545 Fragments arranged by size: 4545 1174 106 80 73 16 15 7 BamHI G'GATC_C Cuts at: 3346 3713 3346 Size: 367 5649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3337 4792 618 Size: 325 217 627 1550 1455 1842 Fragments arranged by size: 1842 1550 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2030 2115 2130 2145 2161 2168 2184 2792 Size: 639 85 15 15 16 7 16 608 Cuts at: 2792 2916 3312 3335 1391 Size: 124 396 23 4072 Fragments arranged by size: 4072 639 608 396 124 85 23 16 16 15 15 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2825 928 Size: 971 926 4119 Fragments arranged by size: 4119 971 926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4207 4241 1903 Size: 34 50 34 2186 34 3678 Fragments arranged by size: 3678 2186 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3908 5118 3908 Size: 1210 4806 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4627 136 Size: 107 122 71 631 605 2955 1525 Fragments arranged by size: 2955 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6011 6011 Size: 6016 BlpI GC'TnA_GC Cuts at: 2432 2525 2996 3433 2432 Size: 93 471 437 5015 Fragments arranged by size: 5015 471 437 93 Bme1580I G_kGCm'C Cuts at: 1790 2168 2205 3265 3304 3312 4077 5233 1790 Size: 378 37 1060 39 8 765 1156 2573 Fragments arranged by size: 2573 1156 1060 765 378 39 37 8 BmgBI CAC'GTC Cuts at: 1318 2461 1318 Size: 1143 4873 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 3232 3747 Size: 416 53 83 399 24 908 907 515 Cuts at: 3747 4705 442 Size: 958 1753 Fragments arranged by size: 1753 958 908 907 515 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2307 2645 2930 3071 4676 1239 Size: 1068 338 285 141 1605 2579 Fragments arranged by size: 2579 1605 1068 338 285 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2209 4141 5006 5304 2209 Size: 1932 865 298 2921 Fragments arranged by size: 2921 1932 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2325 2575 4679 882 Size: 617 826 250 2104 2219 Fragments arranged by size: 2219 2104 826 617 250 BsaAI yAC'GTr Cuts at: 493 1204 2920 3243 3807 493 Size: 711 1716 323 564 2702 Fragments arranged by size: 2702 1716 711 564 323 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4205 4840 275 Size: 53 83 186 936 2290 382 635 1451 Fragments arranged by size: 2290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2115 3041 4449 4847 5121 5268 5679 2115 Size: 926 1408 398 274 147 411 2452 Fragments arranged by size: 2452 1408 926 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2305 2335 1178 Size: 30 165 30 902 30 4859 Fragments arranged by size: 4859 902 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 3060 1186 Size: 1165 709 4142 Fragments arranged by size: 4142 1165 709 BseYI C'CCAG_C Cuts at: 2551 2937 3026 3181 5219 5715 2551 Size: 386 89 155 2038 496 2852 Fragments arranged by size: 2852 2038 496 386 155 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3011 1163 Size: 573 1275 4168 Fragments arranged by size: 4168 1275 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2894 3466 4227 5255 1540 Size: 275 186 893 572 761 1028 2301 Fragments arranged by size: 2301 1028 893 761 572 275 186 BsiHKAI G_wGCw'C Cuts at: 2792 3039 3304 4077 4162 5233 2792 Size: 247 265 773 85 1071 3575 Fragments arranged by size: 3575 1071 773 265 247 85 BsmI GAATG_Cn' Cuts at: 2543 3527 3620 2543 Size: 984 93 4939 Fragments arranged by size: 4939 984 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2451 2451 Size: 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2115 2130 2145 2161 2168 2184 Size: 399 240 85 15 15 16 7 16 Cuts at: 2184 2205 2792 2916 3039 3265 3304 3312 3335 Size: 21 587 124 123 226 39 8 23 Cuts at: 3335 4077 4162 5233 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 587 399 240 226 124 123 85 85 39 23 21 16 16 15 15 8 7 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 3905 5635 3905 Size: 1730 4286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2820 3174 3371 844 Size: 1976 354 197 3489 Fragments arranged by size: 3489 1976 354 197 BsrBI CCG'CTC Cuts at: 1394 1823 2344 3903 1394 Size: 429 521 1559 3507 Fragments arranged by size: 3507 1559 521 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4511 4685 65 Size: 1183 3263 174 1396 Fragments arranged by size: 3263 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4660 4847 1700 Size: 110 2850 187 2869 Fragments arranged by size: 2869 2850 187 110 BsrGI T'GTAC_A Cuts at: 96 2475 96 Size: 2379 3637 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 2621 2735 4076 5088 2621 Size: 114 1341 1012 3549 Fragments arranged by size: 3549 1341 1012 114 BstAPI GCAn_nnn'nTGC Cuts at: 794 2380 2650 794 Size: 1586 270 4160 Fragments arranged by size: 4160 1586 270 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstYI r'GATC_y Cuts at: 1972 2365 3204 3346 3713 4098 4115 5567 5653 Size: 393 839 142 367 385 17 1452 86 Cuts at: 5653 5665 6011 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 839 393 385 367 346 142 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2592 2592 Size: 6016 BtgI C'CryG_G Cuts at: 513 1065 2390 2898 3265 513 Size: 552 1325 508 367 3264 Fragments arranged by size: 3264 1325 552 508 367 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5277 Fragments arranged by size: 5277 683 56 BtsI GCAGTG_nn' Cuts at: 2847 3304 3532 4326 4346 2847 Size: 457 228 794 20 4517 Fragments arranged by size: 4517 794 457 228 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DraIII CAC_nnn'GTG Cuts at: 3271 3271 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2465 5023 809 Size: 507 1149 2558 1802 Fragments arranged by size: 2558 1802 1149 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2727 2891 3463 Size: 54 1425 50 275 18 897 164 572 Cuts at: 3463 4352 5981 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 897 889 572 275 164 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2891 3463 1537 Size: 275 1079 572 4090 Fragments arranged by size: 4090 1079 572 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2149 2426 3946 1353 Size: 796 277 1520 3423 Fragments arranged by size: 3423 1520 796 277 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2790 2790 Size: 6016 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2645 2854 2930 3071 3126 4079 Size: 393 675 338 209 76 141 55 953 Cuts at: 4079 4676 5463 5731 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 953 787 675 597 393 338 268 209 141 76 55 EcoO109I rG'GnC_Cy Cuts at: 2164 2301 3308 3309 2164 Size: 137 1007 1 4871 Fragments arranged by size: 4871 1007 137 1 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 2249 3060 5163 1678 Size: 571 811 2103 2531 Fragments arranged by size: 2531 2103 811 571 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2305 2337 2350 2382 2590 Size: 32 135 32 733 32 13 32 208 Cuts at: 2590 2622 2790 2822 4657 4689 4731 4763 1373 Size: 32 168 32 1835 32 42 32 2626 Fragments arranged by size: 2626 1835 733 208 168 135 42 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 3360 3606 669 Size: 988 281 1422 246 3079 Fragments arranged by size: 3079 1422 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2998 5130 5314 1305 Size: 1693 2132 184 2007 Fragments arranged by size: 2132 2007 1693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2729 10 Size: 54 1425 343 897 3297 Fragments arranged by size: 3297 1425 897 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 3974 4333 4492 518 Size: 648 1347 1461 359 159 2042 Fragments arranged by size: 2042 1461 1347 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2123 2392 2650 2995 3185 3432 4109 5257 Size: 517 269 258 345 190 247 677 1148 Cuts at: 5257 5502 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 517 345 269 258 247 245 190 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5464 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 2476 3374 4919 1132 Size: 97 1247 898 1545 2229 Fragments arranged by size: 2229 1545 1247 898 97 PasI CC'CwG_GG Cuts at: 1348 2638 2927 1348 Size: 1290 289 4437 Fragments arranged by size: 4437 1290 289 PciI A'CATG_T Cuts at: 4915 4915 Size: 6016 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6016 PfoI T'CCnGG_A Cuts at: 2907 2907 Size: 6016 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PmlI CAC'GTG Cuts at: 2920 3243 2920 Size: 323 5693 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PspOMI G'GGCC_C Cuts at: 2164 3308 2164 Size: 1144 4872 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2837 2869 2837 Size: 32 5984 PstI C_TGCA'G Cuts at: 830 2655 2817 3015 3171 3368 830 Size: 1825 162 198 156 197 3478 Fragments arranged by size: 3478 1825 198 197 162 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 PvuII CAG'CTG Cuts at: 2650 2995 3185 2650 Size: 345 190 5481 Fragments arranged by size: 5481 345 190 SacI G_AGCT'C Cuts at: 2792 2792 Size: 6016 SacII CC_GC'GG Cuts at: 2393 2393 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SapI GCTCTTCn'nnn_ Cuts at: 2149 2426 2149 Size: 277 5739 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SexAI A'CCwGG_T Cuts at: 2565 2565 Size: 6016 SfcI C'TryA_G Cuts at: 826 1045 2651 2813 3011 3167 3364 4499 5180 Size: 219 1606 162 198 156 197 1135 681 Cuts at: 5180 5371 826 Size: 191 1471 Fragments arranged by size: 1606 1471 1135 681 219 198 197 191 162 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 2025 2131 2162 3343 1872 Size: 153 106 31 1181 4545 Fragments arranged by size: 4545 1181 153 106 31 SmlI C'TyrA_G Cuts at: 820 1017 1943 2188 4120 5021 5283 820 Size: 197 926 245 1932 901 262 1553 Fragments arranged by size: 1932 1553 926 901 262 245 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SrfI GCCC'GGGC Cuts at: 2025 2131 2025 Size: 106 5910 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6016 StyI C'CwwG_G Cuts at: 513 1065 2956 3114 3195 3455 513 Size: 552 1891 158 81 260 3074 Fragments arranged by size: 3074 1891 552 260 158 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4043 4060 4213 4398 4843 325 Size: 1255 2463 17 153 185 445 1498 Fragments arranged by size: 2463 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 4262 5974 Size: 274 80 33 51 1443 498 1787 1712 Cuts at: 5974 96 Size: 138 Fragments arranged by size: 1787 1712 1443 498 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5976 6008 5976 Size: 32 5984 Tth111I GACn'n_nGTC Cuts at: 1316 2317 1316 Size: 1001 5015 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2905 2924 2905 Size: 19 5997 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 2023 2129 2160 3341 1870 Size: 153 106 31 1181 4545 Fragments arranged by size: 4545 1181 153 106 31 XmnI GAAnn'nnTTC Cuts at: 2867 4145 2867 Size: 1278 4738 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI RsrII SanDI SfiI SfoI SwaI