Restriction map for
pFN21AB7186
(Linear) (Six-base) MAPSORT of: KIBB7186.seq Check: 363 from: 1 to: 1311
KIBB7186.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1169 1311
Size: 1169 142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 1121 1311
Size: 849 272 190
Fragments arranged by size:
849 272 190
AflIII A'CryG_T
Cuts at: 0 914 1311
Size: 914 397
AleI CACnn'nnGTG
Cuts at: 0 508 1311
Size: 508 803
AlwNI CAG_nnn'CTG
Cuts at: 0 426 645 744 872 1311
Size: 426 219 99 128 439
Fragments arranged by size:
439 426 219 128 99
ApaI G_GGCC'C
Cuts at: 0 163 1307 1311
Size: 163 1144 4
Fragments arranged by size:
1144 163 4
ApaLI G'TGCA_C
Cuts at: 0 1295 1311
Size: 1295 16
ApoI r'AATT_y
Cuts at: 0 1117 1311
Size: 1117 194
AvaI C'yCGr_G
Cuts at: 0 18 124 139 155 162 1311
Size: 18 106 15 16 7 1149
Fragments arranged by size:
1149 106 18 16 15 7
BanII G_rGCy'C
Cuts at: 0 25 110 125 140 156 163 179 787
Size: 25 85 15 15 16 7 16 608
Cuts at: 787 911 1307 1311
Size: 124 396 4
Fragments arranged by size:
608 396 124 85 25 16 16 15
15 7 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 820 1311
Size: 820 491
BlpI GC'TnA_GC
Cuts at: 0 427 520 991 1311
Size: 427 93 471 320
Fragments arranged by size:
471 427 320 93
Bme1580I G_kGCm'C
Cuts at: 0 163 200 1260 1299 1307 1311
Size: 163 37 1060 39 8 4
Fragments arranged by size:
1060 163 39 37 8 4
BmgBI CAC'GTC
Cuts at: 0 456 1311
Size: 456 855
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 1227 1311
Size: 320 907 84
Fragments arranged by size:
907 320 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 1311
Size: 90 1221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 640 925 1066 1311
Size: 302 338 285 141 245
Fragments arranged by size:
338 302 285 245 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1311
Size: 204 1107
BsaI GGTCTCn'nnnn_
Cuts at: 0 320 570 1311
Size: 320 250 741
Fragments arranged by size:
741 320 250
BsaAI yAC'GTr
Cuts at: 0 915 1238 1311
Size: 915 323 73
Fragments arranged by size:
915 323 73
BsaWI w'CCGG_w
Cuts at: 0 110 1036 1311
Size: 110 926 275
Fragments arranged by size:
926 275 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 300 330 1311
Size: 300 30 981
Fragments arranged by size:
981 300 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 1055 1311
Size: 346 709 256
Fragments arranged by size:
709 346 256
BseYI C'CCAG_C
Cuts at: 0 546 932 1021 1176 1311
Size: 546 386 89 155 135
Fragments arranged by size:
546 386 155 135 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1311
Size: 1006 305
BsiEI CG_ry'CG
Cuts at: 0 889 1311
Size: 889 422
BsiHKAI G_wGCw'C
Cuts at: 0 787 1034 1299 1311
Size: 787 247 265 12
Fragments arranged by size:
787 265 247 12
BsmI GAATG_Cn'
Cuts at: 0 538 1311
Size: 538 773
BsmBI CGTCTCn'nnnn_
Cuts at: 0 446 1311
Size: 446 865
Bsp1286I G_dGCh'C
Cuts at: 0 25 110 125 140 156 163 179 200
Size: 25 85 15 15 16 7 16 21
Cuts at: 200 787 911 1034 1260 1299 1307 1311
Size: 587 124 123 226 39 8 4
Fragments arranged by size:
587 226 124 123 85 39 25 21
16 16 15 15 8 7 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 815 1169 1311
Size: 815 354 142
Fragments arranged by size:
815 354 142
BsrBI CCG'CTC
Cuts at: 0 339 1311
Size: 339 972
BsrGI T'GTAC_A
Cuts at: 0 470 1311
Size: 470 841
BssSI C'ACGA_G
Cuts at: 0 616 730 1311
Size: 616 114 581
Fragments arranged by size:
616 581 114
BstAPI GCAn_nnn'nTGC
Cuts at: 0 375 645 1311
Size: 375 270 666
Fragments arranged by size:
666 375 270
BstYI r'GATC_y
Cuts at: 0 360 1199 1311
Size: 360 839 112
Fragments arranged by size:
839 360 112
Bsu36I CC'TnA_GG
Cuts at: 0 587 1311
Size: 587 724
BtgI C'CryG_G
Cuts at: 0 385 893 1260 1311
Size: 385 508 367 51
Fragments arranged by size:
508 385 367 51
BtsI GCAGTG_nn'
Cuts at: 0 842 1299 1311
Size: 842 457 12
Fragments arranged by size:
842 457 12
DraIII CAC_nnn'GTG
Cuts at: 0 1266 1311
Size: 1266 45
DrdI GACnn_nn'nnGTC
Cuts at: 0 460 1311
Size: 460 851
EaeI y'GGCC_r
Cuts at: 0 722 886 1311
Size: 722 164 425
Fragments arranged by size:
722 425 164
EagI C'GGCC_G
Cuts at: 0 886 1311
Size: 886 425
EarI CTCTTCn'nnn_
Cuts at: 0 144 421 1311
Size: 144 277 890
Fragments arranged by size:
890 277 144
EcoICRI GAG'CTC
Cuts at: 0 785 1311
Size: 785 526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 640 849 925 1066 1121 1311
Size: 302 338 209 76 141 55 190
Fragments arranged by size:
338 302 209 190 141 76 55
EcoO109I rG'GnC_Cy
Cuts at: 0 159 296 1303 1304 1311
Size: 159 137 1007 1 7
Fragments arranged by size:
1007 159 137 7 1
HaeII r_GCGC'y
Cuts at: 0 244 1055 1311
Size: 244 811 256
Fragments arranged by size:
811 256 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 300 332 345 377 585 617 785 817
Size: 300 32 13 32 208 32 168 32
Cuts at: 817 1311
Size: 494
Fragments arranged by size:
494 300 208 168 32 32 32 32
13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 993 1311
Size: 993 318
MscI TGG'CCA
Cuts at: 0 724 1311
Size: 724 587
MslI CAynn'nnrTG
Cuts at: 0 508 1311
Size: 508 803
MspA1I CmG'CkG
Cuts at: 0 118 387 645 990 1180 1311
Size: 118 269 258 345 190 131
Fragments arranged by size:
345 269 258 190 131 118
NspI r_CATG'y
Cuts at: 0 471 1311
Size: 471 840
PasI CC'CwG_GG
Cuts at: 0 633 922 1311
Size: 633 289 389
Fragments arranged by size:
633 389 289
PfoI T'CCnGG_A
Cuts at: 0 902 1311
Size: 902 409
PmlI CAC'GTG
Cuts at: 0 915 1238 1311
Size: 915 323 73
Fragments arranged by size:
915 323 73
PspOMI G'GGCC_C
Cuts at: 0 159 1303 1311
Size: 159 1144 8
Fragments arranged by size:
1144 159 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 832 864 1311
Size: 832 32 447
Fragments arranged by size:
832 447 32
PstI C_TGCA'G
Cuts at: 0 650 812 1010 1166 1311
Size: 650 162 198 156 145
Fragments arranged by size:
650 198 162 156 145
PvuII CAG'CTG
Cuts at: 0 645 990 1180 1311
Size: 645 345 190 131
Fragments arranged by size:
645 345 190 131
SacI G_AGCT'C
Cuts at: 0 787 1311
Size: 787 524
SacII CC_GC'GG
Cuts at: 0 388 1311
Size: 388 923
SapI GCTCTTCn'nnn_
Cuts at: 0 144 421 1311
Size: 144 277 890
Fragments arranged by size:
890 277 144
SexAI A'CCwGG_T
Cuts at: 0 560 1311
Size: 560 751
SfcI C'TryA_G
Cuts at: 0 646 808 1006 1162 1311
Size: 646 162 198 156 149
Fragments arranged by size:
646 198 162 156 149
SmaI CCC'GGG
Cuts at: 0 20 126 157 1311
Size: 20 106 31 1154
Fragments arranged by size:
1154 106 31 20
SmlI C'TyrA_G
Cuts at: 0 183 1311
Size: 183 1128
SrfI GCCC'GGGC
Cuts at: 0 20 126 1311
Size: 20 106 1185
Fragments arranged by size:
1185 106 20
StyI C'CwwG_G
Cuts at: 0 951 1109 1190 1311
Size: 951 158 81 121
Fragments arranged by size:
951 158 121 81
TatI w'GTAC_w
Cuts at: 0 470 1311
Size: 470 841
Tth111I GACn'n_nGTC
Cuts at: 0 312 1311
Size: 312 999
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 900 919 1311
Size: 900 19 392
Fragments arranged by size:
900 392 19
XmaI C'CCGG_G
Cuts at: 0 18 124 155 1311
Size: 18 106 31 1156
Fragments arranged by size:
1156 106 31 18
XmnI GAAnn'nnTTC
Cuts at: 0 862 1311
Size: 862 449
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI ApoI
AvaI BanII BbsI BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I MmeI MscI MslI MspA1I NspI PasI PfoI
PmlI PspOMI PsrI PstI PvuII SacI SacII SapI
SexAI SfcI SmaI SmlI SrfI StyI TatI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmtI Bpu10I BsaBI BsaHI BsiWI BspEI BspHI
BsrDI BsrFI BssHII BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PvuI RsrII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TaqII TstI XbaI XhoI
ZraI