DDX56 ORF clone FHC01461

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01461

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Clone info.

Species

human

Clone Name

pFN21AB5068 Sequence (with vector) Restriction Map

Product ID

FHC01461

Flexi Clone PID

FXC01461

Original Clone PID

ORK01461

ORF Sequence

DNA sequence (1641 bp)
Protein sequence (547 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385026]

Gene

KIBB5068

Symbol

DDX56

Alias : DDX21, DDX26, NOH61

Description

DEAD (Asp-Glu-Ala-Asp) box helicase 56, transcript variant 1

Original Clone ID

bm05962

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1641 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 547 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5492.1 9.3e-218 100.0 DDX56 CCDS59053.1 1.5e-111 92.7 DDX56 CCDS13416.1 8.5e-30 28.7 DDX27 CCDS2120.1 9.6e-23 28.5 DDX18 CCDS32704.1 1.1e-21 27.7 DDX42 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 32 206 PF00270 DNA/RNA helicase IPR001650 276 319 PF00271 Helicase IPR001650 344 380 PF00271 Helicase HMMSmart IPR014001 26 234 SM00487 DEAD-like helicase IPR001650 272 380 SM00490 Helicase ProfileScan IPR014014 7 35 PS51195 RNA helicase IPR014001 38 218 PS51192 DEAD-like helicase IPR001650 230 424 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_061955 547 608023 100.0 100.0 NP_001244118 507 608023 92.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5068 Download

>KIBB5068  1641 bp
ATGGAGGACTCTGAAGCACTGGGCTTCGAACACATGGGCCTCGATCCCCGGCTCCTTCAG
GCTGTCACCGATCTGGGCTGGTCGCGACCTACGCTGATCCAGGAGAAGGCCATCCCACTG
GCCCTAGAAGGGAAGGACCTCCTGGCTCGGGCCCGCACGGGCTCCGGGAAGACGGCCGCT
TATGCTATTCCGATGCTGCAGCTGTTGCTCCATAGGAAGGCGACAGGTCCGGTGGTAGAA
CAGGCAGTGAGAGGCCTTGTTCTTGTTCCTACCAAGGAGCTGGCACGGCAAGCACAGTCC
ATGATTCAGCAGCTGGCTACCTACTGTGCTCGGGATGTCCGAGTGGCCAATGTCTCAGCT
GCTGAAGACTCAGTCTCTCAGAGAGCTGTGCTGATGGAGAAGCCAGATGTGGTAGTAGGG
ACCCCATCTCGCATATTAAGCCACTTGCAGCAAGACAGCCTGAAACTTCGTGACTCCCTG
GAGCTTTTGGTGGTGGACGAAGCTGACCTTCTTTTTTCCTTTGGCTTTGAAGAAGAGCTC
AAGAGTCTCCTCTGTCACTTGCCCCGGATTTACCAGGCTTTTCTCATGTCAGCTACTTTT
AACGAGGACGTACAAGCACTCAAGGAGCTGATATTACATAACCCGGTTACCCTTAAGTTA
CAGGAGTCCCAGCTGCCTGGGCCAGACCAGTTACAGCAGTTTCAGGTGGTCTGTGAGACT
GAGGAAGACAAATTCCTCCTGCTGTATGCCCTGCTCAAGCTGTCATTGATTCGGGGCAAG
TCTCTGCTCTTTGTCAACACTCTAGAACGGAGTTACCGGCTACGCCTGTTCTTGGAACAG
TTCAGCATCCCCACCTGTGTGCTCAATGGAGAGCTTCCACTGCGCTCCAGGTGCCACATC
ATCTCACAGTTCAACCAAGGCTTCTACGACTGTGTCATAGCAACTGATGCTGAAGTCCTG
GGGGCCCCAGTCAAGGGCAAGCGTCGGGGCCGAGGGCCCAAAGGGGACAAGGCCTCTGAT
CCGGAAGCAGGTGTGGCCCGGGGCATAGACTTCCACCATGTGTCTGCTGTGCTCAACTTT
GATCTTCCCCCAACCCCTGAGGCCTACATCCATCGAGCTGGCAGGACAGCACGCGCTAAC
AACCCAGGCATAGTCTTAACCTTTGTGCTTCCCACGGAGCAGTTCCACTTAGGCAAGATT
GAGGAGCTTCTCAGTGGAGAGAACAGGGGCCCCATTCTGCTCCCCTACCAGTTCCGGATG
GAGGAGATCGAGGGCTTCCGCTATCGCTGCAGGGATGCCATGCGCTCAGTGACTAAGCAG
GCCATTCGGGAGGCAAGATTGAAGGAGATCAAGGAAGAGCTTCTGCATTCTGAGAAGCTT
AAGACATACTTTGAAGACAACCCTAGGGACCTCCAGCTGCTGCGGCATGACCTACCTTTG
CACCCCGCAGTGGTGAAGCCCCACCTGGGCCATGTTCCTGACTACCTGGTTCCTCCTGCT
CTCCGTGGCCTGGTGCGCCCTCACAAGAAGCGGAAGAAGCTGTCTTCCTCTTGTAGGAAG
GCCAAGAGAGCAAAGTCCCAGAACCCACTGCGCAGCTTCAAGCACAAAGGAAAGAAATTC
AGACCCACAGCCAAGCCCTCC

ORF Protein

Cloned ORF protein sequence for pFN21AB5068 Download

>KIBB5068  547 aa
MEDSEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAA
YAIPMLQLLLHRKATGPVVEQAVRGLVLVPTKELARQAQSMIQQLATYCARDVRVANVSA
AEDSVSQRAVLMEKPDVVVGTPSRILSHLQQDSLKLRDSLELLVVDEADLLFSFGFEEEL
KSLLCHLPRIYQAFLMSATFNEDVQALKELILHNPVTLKLQESQLPGPDQLQQFQVVCET
EEDKFLLLYALLKLSLIRGKSLLFVNTLERSYRLRLFLEQFSIPTCVLNGELPLRSRCHI
ISQFNQGFYDCVIATDAEVLGAPVKGKRRGRGPKGDKASDPEAGVARGIDFHHVSAVLNF
DLPPTPEAYIHRAGRTARANNPGIVLTFVLPTEQFHLGKIEELLSGENRGPILLPYQFRM
EEIEGFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPL
HPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKF
RPTAKPS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5068 Download

>pFN21AB5068  6346 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGGACTCTGAAGCACTGGGCTTCGAACACAT
GGGCCTCGATCCCCGGCTCCTTCAGGCTGTCACCGATCTGGGCTGGTCGCGACCTACGCT
GATCCAGGAGAAGGCCATCCCACTGGCCCTAGAAGGGAAGGACCTCCTGGCTCGGGCCCG
CACGGGCTCCGGGAAGACGGCCGCTTATGCTATTCCGATGCTGCAGCTGTTGCTCCATAG
GAAGGCGACAGGTCCGGTGGTAGAACAGGCAGTGAGAGGCCTTGTTCTTGTTCCTACCAA
GGAGCTGGCACGGCAAGCACAGTCCATGATTCAGCAGCTGGCTACCTACTGTGCTCGGGA
TGTCCGAGTGGCCAATGTCTCAGCTGCTGAAGACTCAGTCTCTCAGAGAGCTGTGCTGAT
GGAGAAGCCAGATGTGGTAGTAGGGACCCCATCTCGCATATTAAGCCACTTGCAGCAAGA
CAGCCTGAAACTTCGTGACTCCCTGGAGCTTTTGGTGGTGGACGAAGCTGACCTTCTTTT
TTCCTTTGGCTTTGAAGAAGAGCTCAAGAGTCTCCTCTGTCACTTGCCCCGGATTTACCA
GGCTTTTCTCATGTCAGCTACTTTTAACGAGGACGTACAAGCACTCAAGGAGCTGATATT
ACATAACCCGGTTACCCTTAAGTTACAGGAGTCCCAGCTGCCTGGGCCAGACCAGTTACA
GCAGTTTCAGGTGGTCTGTGAGACTGAGGAAGACAAATTCCTCCTGCTGTATGCCCTGCT
CAAGCTGTCATTGATTCGGGGCAAGTCTCTGCTCTTTGTCAACACTCTAGAACGGAGTTA
CCGGCTACGCCTGTTCTTGGAACAGTTCAGCATCCCCACCTGTGTGCTCAATGGAGAGCT
TCCACTGCGCTCCAGGTGCCACATCATCTCACAGTTCAACCAAGGCTTCTACGACTGTGT
CATAGCAACTGATGCTGAAGTCCTGGGGGCCCCAGTCAAGGGCAAGCGTCGGGGCCGAGG
GCCCAAAGGGGACAAGGCCTCTGATCCGGAAGCAGGTGTGGCCCGGGGCATAGACTTCCA
CCATGTGTCTGCTGTGCTCAACTTTGATCTTCCCCCAACCCCTGAGGCCTACATCCATCG
AGCTGGCAGGACAGCACGCGCTAACAACCCAGGCATAGTCTTAACCTTTGTGCTTCCCAC
GGAGCAGTTCCACTTAGGCAAGATTGAGGAGCTTCTCAGTGGAGAGAACAGGGGCCCCAT
TCTGCTCCCCTACCAGTTCCGGATGGAGGAGATCGAGGGCTTCCGCTATCGCTGCAGGGA
TGCCATGCGCTCAGTGACTAAGCAGGCCATTCGGGAGGCAAGATTGAAGGAGATCAAGGA
AGAGCTTCTGCATTCTGAGAAGCTTAAGACATACTTTGAAGACAACCCTAGGGACCTCCA
GCTGCTGCGGCATGACCTACCTTTGCACCCCGCAGTGGTGAAGCCCCACCTGGGCCATGT
TCCTGACTACCTGGTTCCTCCTGCTCTCCGTGGCCTGGTGCGCCCTCACAAGAAGCGGAA
GAAGCTGTCTTCCTCTTGTAGGAAGGCCAAGAGAGCAAAGTCCCAGAACCCACTGCGCAG
CTTCAAGCACAAAGGAAAGAAATTCAGACCCACAGCCAAGCCCTCCGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT