Restriction map for
pFN21AB5068
(Linear) (Six-base) MAPSORT of: KIBB5068.seq Check: 118 from: 1 to: 1641
KIBB5068.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1018 1641
Size: 1018 623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 40 383 971 1641
Size: 32 8 343 588 670
Fragments arranged by size:
670 588 343 32 8
AflII C'TTAA_G
Cuts at: 0 652 1378 1641
Size: 652 726 263
Fragments arranged by size:
726 652 263
AleI CACnn'nnGTG
Cuts at: 0 856 1641
Size: 856 785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 799 1641
Size: 799 842
AlwNI CAG_nnn'CTG
Cuts at: 0 312 361 1419 1641
Size: 312 49 1058 222
Fragments arranged by size:
1058 312 222 49
ApaI G_GGCC'C
Cuts at: 0 153 966 998 1231 1641
Size: 153 813 32 233 410
Fragments arranged by size:
813 410 233 153 32
ApoI r'AATT_y
Cuts at: 0 730 1615 1641
Size: 730 885 26
Fragments arranged by size:
885 730 26
AvaI C'yCGr_G
Cuts at: 0 146 329 1037 1641
Size: 146 183 708 604
Fragments arranged by size:
708 604 183 146
AvrII C'CTAG_G
Cuts at: 0 1402 1641
Size: 1402 239
BanI G'GyrC_C
Cuts at: 0 890 1641
Size: 890 751
BanII G_rGCy'C
Cuts at: 0 153 163 539 966 998 1231 1641
Size: 153 10 376 427 32 233 410
Fragments arranged by size:
427 410 376 233 153 32 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 175 371 731 1400 1535 1641
Size: 175 196 360 669 135 106
Fragments arranged by size:
669 360 196 175 135 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1306 1340 1641
Size: 1306 34 301
Fragments arranged by size:
1306 301 34
Bme1580I G_kGCm'C
Cuts at: 0 153 966 998 1231 1641
Size: 153 813 32 233 410
Fragments arranged by size:
813 410 233 153 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 28 961 1641
Size: 28 933 680
Fragments arranged by size:
933 680 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 870 1396 1641
Size: 499 371 526 245
Fragments arranged by size:
526 499 371 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 604 739 1641
Size: 523 81 135 902
Fragments arranged by size:
902 523 135 81
BsaWI w'CCGG_w
Cuts at: 0 228 1020 1253 1641
Size: 228 792 233 388
Fragments arranged by size:
792 388 233 228
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 1216 1276 1641
Size: 538 678 60 365
Fragments arranged by size:
678 538 365 60
BseYI C'CCAG_C
Cuts at: 0 668 1641
Size: 668 973
BsiEI CG_ry'CG
Cuts at: 0 176 1641
Size: 176 1465
BsiHKAI G_wGCw'C
Cuts at: 0 330 539 863 1073 1641
Size: 330 209 324 210 568
Fragments arranged by size:
568 330 324 210 209
BsmI GAATG_Cn'
Cuts at: 0 1365 1641
Size: 1365 276
Bsp1286I G_dGCh'C
Cuts at: 0 153 163 330 539 863 966 998 1073
Size: 153 10 167 209 324 103 32 75
Cuts at: 1073 1231 1641
Size: 158 410
Fragments arranged by size:
410 324 209 167 158 153 103 75
32 10
BspEI T'CCGG_A
Cuts at: 0 1020 1253 1641
Size: 1020 233 388
Fragments arranged by size:
1020 388 233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1018 1641
Size: 1018 623
BsrFI r'CCGG_y
Cuts at: 0 815 1641
Size: 815 826
BstAPI GCAn_nnn'nTGC
Cuts at: 0 204 946 1641
Size: 204 742 695
Fragments arranged by size:
742 695 204
BstBI TT'CG_AA
Cuts at: 0 26 1641
Size: 26 1615
BstEII G'GTnAC_C
Cuts at: 0 645 1641
Size: 645 996
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 279 676 1117 1641
Size: 117 162 397 441 524
Fragments arranged by size:
524 441 397 162 117
Bsu36I CC'TnA_GG
Cuts at: 0 1097 1641
Size: 1097 544
BtgI C'CryG_G
Cuts at: 0 1172 1503 1641
Size: 1172 331 138
Fragments arranged by size:
1172 331 138
BtsI GCAGTG_nn'
Cuts at: 0 251 877 1454 1585 1641
Size: 251 626 577 131 56
Fragments arranged by size:
626 577 251 131 56
DraIII CAC_nnn'GTG
Cuts at: 0 1059 1641
Size: 1059 582
EaeI y'GGCC_r
Cuts at: 0 173 344 1641
Size: 173 171 1297
Fragments arranged by size:
1297 173 171
EagI C'GGCC_G
Cuts at: 0 173 1641
Size: 173 1468
EarI CTCTTCn'nnn_
Cuts at: 0 527 1349 1641
Size: 527 822 292
Fragments arranged by size:
822 527 292
EcoICRI GAG'CTC
Cuts at: 0 537 1641
Size: 537 1104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 40 383 499 870 971 1396 1641
Size: 32 8 343 116 371 101 425 245
Fragments arranged by size:
425 371 343 245 116 101 32 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1551 1641
Size: 1551 90
EcoO109I rG'GnC_Cy
Cuts at: 0 135 419 962 963 994 1227 1228 1407
Size: 135 284 543 1 31 233 1 179
Cuts at: 1407 1641
Size: 234
Fragments arranged by size:
543 284 234 233 179 135 31 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 523 1404 1641
Size: 523 881 237
Fragments arranged by size:
881 523 237
FspI TGC'GCA
Cuts at: 0 1591 1641
Size: 1591 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 411 443 997 1029 1641
Size: 358 32 21 32 554 32 612
Fragments arranged by size:
612 554 358 32 32 32 21
HincII GTy'rAC
Cuts at: 0 795 1641
Size: 795 846
HindIII A'AGCT_T
Cuts at: 0 1375 1641
Size: 1375 266
MscI TGG'CCA
Cuts at: 0 346 1641
Size: 346 1295
MslI CAynn'nnrTG
Cuts at: 0 856 1641
Size: 856 785
MspA1I CmG'CkG
Cuts at: 0 201 312 358 672 1416 1641
Size: 201 111 46 314 744 225
Fragments arranged by size:
744 314 225 201 111 46
NruI TCG'CGA
Cuts at: 0 84 1641
Size: 84 1557
PfoI T'CCnGG_A
Cuts at: 0 98 163 1641
Size: 98 65 1478
Fragments arranged by size:
1478 98 65
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 242 274 1641
Size: 242 32 1367
Fragments arranged by size:
1367 242 32
PpuMI rG'GwC_Cy
Cuts at: 0 135 419 1407 1641
Size: 135 284 988 234
Fragments arranged by size:
988 284 234 135
PspOMI G'GGCC_C
Cuts at: 0 149 962 994 1227 1641
Size: 149 813 32 233 414
Fragments arranged by size:
813 414 233 149 32
PstI C_TGCA'G
Cuts at: 0 200 1291 1641
Size: 200 1091 350
Fragments arranged by size:
1091 350 200
PvuII CAG'CTG
Cuts at: 0 201 312 358 672 1416 1641
Size: 201 111 46 314 744 225
Fragments arranged by size:
744 314 225 201 111 46
SacI G_AGCT'C
Cuts at: 0 539 1641
Size: 539 1102
SanDI GG'GwC_CC
Cuts at: 0 419 1641
Size: 419 1222
SapI GCTCTTCn'nnn_
Cuts at: 0 527 1349 1641
Size: 527 822 292
Fragments arranged by size:
822 527 292
SexAI A'CCwGG_T
Cuts at: 0 1484 1641
Size: 1484 157
SfcI C'TryA_G
Cuts at: 0 196 1287 1641
Size: 196 1091 354
Fragments arranged by size:
1091 354 196
SmaI CCC'GGG
Cuts at: 0 1039 1641
Size: 1039 602
SmlI C'TyrA_G
Cuts at: 0 538 619 652 754 1378 1641
Size: 538 81 33 102 624 263
Fragments arranged by size:
624 538 263 102 81 33
StuI AGG'CCT
Cuts at: 0 254 1012 1102 1641
Size: 254 758 90 539
Fragments arranged by size:
758 539 254 90
StyI C'CwwG_G
Cuts at: 0 272 915 1402 1641
Size: 272 643 487 239
Fragments arranged by size:
643 487 272 239
Tth111I GACn'n_nGTC
Cuts at: 0 370 931 1641
Size: 370 561 710
Fragments arranged by size:
710 561 370
XbaI T'CTAG_A
Cuts at: 0 801 1641
Size: 801 840
XmaI C'CCGG_G
Cuts at: 0 1037 1641
Size: 1037 604
XmnI GAAnn'nnTTC
Cuts at: 0 1358 1641
Size: 1358 283
Enzymes that do cut:
AarI AcuI AflII AleI AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbsI BcgI Bme1580I BmrI
BpmI BpuEI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrFI BstAPI BstBI BstEII BstXI
Bsu36I BtgI BtsI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI Hin4I HincII HindIII
MscI MslI MspA1I NruI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SanDI SapI SexAI SfcI SmaI
SmlI StuI StyI Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI
BsgI BsiWI BsmBI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI
EciI EcoRI EcoRV FseI FspAI HaeII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI TstI XcmI XhoI ZraI