Restriction map for
pFN21AB5068
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI BamHI BbeI BbrPI BbvCI
BciVI BclI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsgI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstYI BstZ17I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FbaI FseI HaeII Hin1I HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NsiI NspI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Van91I
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 45 enzymes) #
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AfaI 610 GTAC GT^AC
AocI 1096 CCTGAGG CC^T[ACGT]AGG
Asp700I 1354 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1589 TGCGCA TGC^GCA
AvrII 1402 CCTAGG C^CTAGG
BalI 344 TGGCCA TGG^CCA
BanI 890 GGTGCC G^G[CT][GA]CC
BcgI 1317 GCAGGCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1402 CCTAGG C^CTAGG
BsiEI 173 CGGCCG CG[GA][CT]^CG
BsmI 1365 GCATTC GAATGC(1/-1)
BspMI 1027 GCAGGT ACCTGC(4/8)
BsrFI 815 ACCGGC [GA]^CCGG[CT]
BstBI 25 TTCGAA TT^CGAA
BstEII 645 GGTTACC G^GT[ACGT]ACC
BstPI 645 GGTTACC G^GT[ACGT]ACC
Bsu36I 1096 CCTGAGG CC^T[ACGT]AGG
Cfr10I 815 ACCGGC [GA]^CCGG[CT]
DraIII 1054 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 173 CGGCCG C^GGCCG
EclXI 173 CGGCCG C^GGCCG
Eco52I 173 CGGCCG C^GGCCG
Eco81I 1096 CCTGAGG CC^T[ACGT]AGG
EcoNI 1547 CCTCTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 645 GGTTACC G^GT[ACGT]ACC
FspI 1589 TGCGCA TGC^GCA
HgaI 981 GCGTC GACGC(5/10)
HincII 793 GTCAAC GT[CT]^[GA]AC
HindII 793 GTCAAC GT[CT]^[GA]AC
HindIII 1375 AAGCTT A^AGCTT
MaeII 608 ACGT A^CGT
MluNI 344 TGGCCA TGG^CCA
MscI 344 TGGCCA TGG^CCA
MslI 852 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 82 TCGCGA TCG^CGA
NspV 25 TTCGAA TT^CGAA
RsaI 610 GTAC GT^AC
SacI 535 GAGCTC GAGCT^C
SexAI 1484 ACCTGGT A^CC[AT]GGT
SfuI 25 TTCGAA TT^CGAA
SmaI 1037 CCCGGG CCC^GGG
XbaI 801 TCTAGA T^CTAGA
XmaCI 1037 CCCGGG C^CCGGG
XmaI 1037 CCCGGG C^CCGGG
XmnI 1354 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 83 CGCG CG^CG
1132 CGCG
AccIII 1020 TCCGGA T^CCGGA
1253 TCCGGA
AcsI 730 AAATTC [GA]^AATT[CT]
1615 AAATTC
AflII 652 CTTAAG C^TTAAG
1378 CTTAAG
ApoI 730 AAATTC [GA]^AATT[CT]
1615 AAATTC
AspI 367 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
928 GACTGTGTC
BfrI 652 CTTAAG C^TTAAG
1378 CTTAAG
BseAI 1020 TCCGGA T^CCGGA
1253 TCCGGA
BspEI 1020 TCCGGA T^CCGGA
1253 TCCGGA
BstAPI 198 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
940 GCAACTGATGC
BstUI 83 CGCG CG^CG
1132 CGCG
DsaI 1172 CCACGG C^C[GA][CT]GG
1503 CCGTGG
EaeI 173 CGGCCG [CT]^GGCC[GA]
344 TGGCCA
EarI 532 GAAGAG CTCTTC(1/4)
1354 GAAGAG
FauI 152 CCCGC CCCGC(4/6)
1444 CCCGC
HphI 1452 GGTGA GGTGA(8/7)
65 TCACC
Ksp632I 532 GAAGAG CTCTTC(1/4)
1354 GAAGAG
MroI 1020 TCCGGA T^CCGGA
1253 TCCGGA
MvnI 83 CGCG CG^CG
1132 CGCG
PflFI 367 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
928 GACTGTGTC
PstI 196 CTGCAG CTGCA^G
1287 CTGCAG
SapI 532 GAAGAGC GCTCTTC(1/4)
1354 GAAGAGC
SfcI 196 CTGCAG C^T[GA][CT]AG
1287 CTGCAG
TfiI 303 GATTC G^A[AT]TC
768 GATTC
Tsp509I 731 AATT ^AATT
1616 AATT
Tth111I 367 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
928 GACTGTGTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I