(Circular) (Six-base) MAPSORT of: pFN21AA1516.seq Check: 1996 from: 1 to: 11611 pFN21AA1516 11611 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 8707 8707 Size: 11611 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5498 7692 9421 278 Size: 53 83 186 936 3962 2194 1729 2468 Fragments arranged by size: 3962 2468 2194 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 3663 4836 7686 8954 1937 Size: 1089 637 1173 2850 1268 4594 Fragments arranged by size: 4594 2850 1268 1173 1089 637 Acc65I G'GTAC_C Cuts at: 8932 8932 Size: 11611 AclI AA'CG_TT Cuts at: 1563 9738 10111 1563 Size: 8175 373 3063 Fragments arranged by size: 8175 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 4046 4154 4640 4922 4984 5723 5789 Size: 402 2012 108 486 282 62 739 66 Cuts at: 5789 5969 6833 7886 9674 11058 11326 1632 Size: 180 864 1053 1788 1384 268 1917 Fragments arranged by size: 2012 1917 1788 1384 1053 864 739 486 402 282 268 180 108 66 62 AflII C'TTAA_G Cuts at: 820 1017 8702 820 Size: 197 7685 3729 Fragments arranged by size: 7685 3729 197 AflIII A'CryG_T Cuts at: 1203 4079 10510 11487 1203 Size: 2876 6431 977 1327 Fragments arranged by size: 6431 2876 1327 977 AgeI A'CCGG_T Cuts at: 3404 10442 3404 Size: 7038 4573 AhdI GACnn_n'nnGTC Cuts at: 10340 10340 Size: 11611 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 11611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3671 5749 1184 Size: 2487 2078 7046 Fragments arranged by size: 7046 2487 2078 AlwNI CAG_nnn'CTG Cuts at: 2030 2297 4585 4913 5071 7330 7357 7777 8128 Size: 267 2288 328 158 2259 27 420 351 Cuts at: 8128 9354 10926 2030 Size: 1226 1572 2715 Fragments arranged by size: 2715 2288 2259 1572 1226 420 351 328 267 158 27 ApaLI G'TGCA_C Cuts at: 4574 9668 10824 4574 Size: 5094 1156 5361 Fragments arranged by size: 5361 5094 1156 ApoI r'AATT_y Cuts at: 1493 2421 2457 4282 4760 5045 5654 6139 6501 Size: 928 36 1825 478 285 609 485 362 Cuts at: 6501 6539 6947 7031 7975 8136 8280 8693 8920 Size: 38 408 84 944 161 144 413 227 Cuts at: 8920 9146 9298 11407 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1825 1697 944 928 609 485 478 413 408 362 285 227 226 161 152 144 84 38 36 AseI AT'TA_AT Cuts at: 160 1032 5382 8100 10165 160 Size: 872 4350 2718 2065 1606 Fragments arranged by size: 4350 2718 2065 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11611 AvaI C'yCGr_G Cuts at: 1870 1943 8730 8802 8936 1870 Size: 73 6787 72 134 4545 Fragments arranged by size: 6787 4545 134 73 72 BamHI G'GATC_C Cuts at: 3470 7603 8941 9308 3470 Size: 4133 1338 367 5773 Fragments arranged by size: 5773 4133 1338 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4160 4972 8108 8932 10387 Size: 325 217 627 2373 812 3136 824 1455 Cuts at: 10387 618 Size: 1842 Fragments arranged by size: 3136 2373 1842 1455 824 812 627 325 217 BanII G_rGCy'C Cuts at: 1391 3716 3821 4143 4539 5452 5552 7133 8082 Size: 2325 105 322 396 913 100 1581 949 Cuts at: 8082 8399 8930 1391 Size: 317 531 4072 Fragments arranged by size: 4072 2325 1581 949 913 531 396 322 317 105 100 BbeI G_GCGC'C Cuts at: 4164 4164 Size: 11611 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2406 2946 5526 6819 8612 928 Size: 971 507 540 2580 1293 1793 3927 Fragments arranged by size: 3927 2580 1793 1293 971 540 507 BbvCI CC'TCA_GC Cuts at: 2394 6044 6480 2394 Size: 3650 436 7525 Fragments arranged by size: 7525 3650 436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6284 6318 7666 7700 8711 8740 8745 Size: 34 4347 34 1348 34 1011 29 5 Cuts at: 8745 8774 9802 9836 1903 Size: 29 1028 34 3678 Fragments arranged by size: 4347 3678 1348 1028 1011 34 34 34 34 29 29 5 BciVI GTATCCnnnnn_n' Cuts at: 3216 7422 8303 8855 9503 10713 3216 Size: 4206 881 552 648 1210 4114 Fragments arranged by size: 4206 4114 1210 881 648 552 BclI T'GATC_A Cuts at: 1548 2517 2764 6958 1548 Size: 969 247 4194 6201 Fragments arranged by size: 6201 4194 969 247 BfrBI ATG'CAT Cuts at: 7058 7058 Size: 11611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 3507 4851 Size: 107 122 71 631 605 403 1432 1344 Cuts at: 4851 10222 136 Size: 5371 1525 Fragments arranged by size: 5371 1525 1432 1344 631 605 403 122 107 71 BglII A'GATC_T Cuts at: 3790 5818 7588 7798 11606 3790 Size: 2028 1770 210 3808 3795 Fragments arranged by size: 3808 3795 2028 1770 210 BlpI GC'TnA_GC Cuts at: 4749 9028 4749 Size: 4279 7332 Bme1580I G_kGCm'C Cuts at: 1790 3698 4087 4578 5733 7652 9672 10828 1790 Size: 1908 389 491 1155 1919 2020 1156 2573 Fragments arranged by size: 2573 2020 1919 1908 1156 1155 491 389 BmgBI CAC'GTC Cuts at: 1318 3429 7233 1318 Size: 2111 3804 5696 Fragments arranged by size: 5696 3804 2111 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2065 3193 4365 Size: 416 53 83 399 24 648 1128 1172 Cuts at: 4365 4781 4898 5949 6947 7647 9342 10300 442 Size: 416 117 1051 998 700 1695 958 1753 Fragments arranged by size: 1753 1695 1172 1128 1051 998 958 700 648 416 416 399 117 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11611 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3835 5973 3835 Size: 2138 9473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4367 4372 4634 7515 8424 8869 10271 1239 Size: 3128 5 262 2881 909 445 1402 2579 Fragments arranged by size: 3128 2881 2579 1402 909 445 262 5 Bpu10I CC'TnA_GC Cuts at: 2394 6044 6480 8540 2394 Size: 3650 436 2060 5465 Fragments arranged by size: 5465 3650 2060 436 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5358 8413 8756 9736 10601 10899 5358 Size: 3055 343 980 865 298 6070 Fragments arranged by size: 6070 3055 980 865 343 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2107 4004 4175 10274 882 Size: 617 608 1897 171 6099 2219 Fragments arranged by size: 6099 2219 1897 617 608 171 BsaAI yAC'GTr Cuts at: 493 1204 4082 4552 9402 493 Size: 711 2878 470 4850 2702 Fragments arranged by size: 4850 2878 2702 711 470 BsaBI GATnn'nnATC Cuts at: 6347 11605 6347 Size: 5258 6353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4161 5495 6644 7689 Size: 53 83 186 936 2628 1334 1149 1045 Cuts at: 7689 9418 9800 10435 275 Size: 1729 382 635 1451 Fragments arranged by size: 2628 1729 1451 1334 1149 1045 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3404 4166 4326 10044 10442 10716 10863 11274 3404 Size: 762 160 5718 398 274 147 411 3741 Fragments arranged by size: 5718 3741 762 411 398 274 160 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2977 3007 3169 3199 4148 Size: 30 165 30 1574 30 162 30 949 Cuts at: 4148 4178 4280 4310 5485 5515 5557 5587 8642 Size: 30 102 30 1175 30 42 30 3055 Cuts at: 8642 8672 1178 Size: 30 4117 Fragments arranged by size: 4117 3055 1574 1175 949 165 162 102 42 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2687 3875 4033 4373 6773 8282 8417 8420 Size: 1501 1188 158 340 2400 1509 135 3 Cuts at: 8420 8873 1186 Size: 453 3924 Fragments arranged by size: 3924 2400 1509 1501 1188 453 340 158 135 3 BseYI C'CCAG_C Cuts at: 3283 3551 4041 4705 5180 5468 6324 7335 8337 Size: 268 490 664 475 288 856 1011 1002 Cuts at: 8337 10814 11310 3283 Size: 2477 496 3584 Fragments arranged by size: 3584 2477 1011 1002 856 664 496 490 475 288 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4073 6010 6716 8469 8706 8741 1163 Size: 573 2337 1937 706 1753 237 35 4033 Fragments arranged by size: 4033 2337 1937 1753 706 573 237 35 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9061 9822 10850 1540 Size: 275 186 7060 761 1028 2301 Fragments arranged by size: 7060 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2539 3716 3777 3821 4143 4539 4578 5173 5954 Size: 1177 61 44 322 396 39 595 781 Cuts at: 5954 8399 9672 9757 10828 2539 Size: 2445 1273 85 1071 3322 Fragments arranged by size: 3322 2445 1273 1177 1071 781 595 396 322 85 61 44 39 BsmI GAATG_Cn' Cuts at: 3710 5209 5413 6721 7185 9122 9215 3710 Size: 1499 204 1308 464 1937 93 6106 Fragments arranged by size: 6106 1937 1499 1308 464 204 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6327 6327 Size: 11611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2539 3698 3716 3777 3821 4087 4143 Size: 399 749 1159 18 61 44 266 56 Cuts at: 4143 4539 4578 5173 5452 5552 5733 5954 7133 Size: 396 39 595 279 100 181 221 1179 Cuts at: 7133 7652 8082 8399 8930 9672 9757 10828 1391 Size: 519 430 317 531 742 85 1071 2174 Fragments arranged by size: 2174 1179 1159 1071 749 742 595 531 519 430 399 396 317 279 266 221 181 100 85 61 56 44 39 18 BspEI T'CCGG_A Cuts at: 4326 11274 4326 Size: 6948 4663 BspHI T'CATG_A Cuts at: 4173 5277 9500 11230 4173 Size: 1104 4223 1730 4554 Fragments arranged by size: 4554 4223 1730 1104 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6447 6739 7305 8707 8966 844 Size: 5603 292 566 1402 259 3489 Fragments arranged by size: 5603 3489 1402 566 292 259 BsrBI CCG'CTC Cuts at: 1394 1823 9498 1394 Size: 429 7675 3507 Fragments arranged by size: 7675 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3341 4922 5907 6473 7494 10106 10280 Size: 1183 2093 1581 985 566 1021 2612 174 Cuts at: 10280 65 Size: 1396 Fragments arranged by size: 2612 2093 1581 1396 1183 1021 985 566 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3404 10255 10442 1700 Size: 110 1594 6851 187 2869 Fragments arranged by size: 6851 2869 1594 187 110 BsrGI T'GTAC_A Cuts at: 96 2379 96 Size: 2283 9328 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11611 BssSI C'ACGA_G Cuts at: 6002 9671 10683 6002 Size: 3669 1012 6930 Fragments arranged by size: 6930 3669 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5071 5204 8128 794 Size: 4277 133 2924 4277 Fragments arranged by size: 4277 4277 2924 133 BstBI TT'CG_AA Cuts at: 7794 10415 7794 Size: 2621 8990 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11611 BstXI CCAn_nnnn'nTGG Cuts at: 2348 3433 7539 2348 Size: 1085 4106 6420 Fragments arranged by size: 6420 4106 1085 BstYI r'GATC_y Cuts at: 1972 3254 3470 3790 4813 5707 5818 6381 7588 Size: 1282 216 320 1023 894 111 563 1207 Cuts at: 7588 7603 7798 8941 9308 9693 9710 11162 11248 Size: 15 195 1143 367 385 17 1452 86 Cuts at: 11248 11260 11606 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1282 1207 1143 1023 894 563 385 367 346 320 216 195 111 86 17 15 12 BstZ17I GTA'TAC Cuts at: 3027 4837 3027 Size: 1810 9801 Bsu36I CC'TnA_GG Cuts at: 3956 3956 Size: 11611 BtgI C'CryG_G Cuts at: 513 1065 2484 3301 3855 4187 4525 4599 7423 Size: 552 1419 817 554 332 338 74 2824 Cuts at: 7423 513 Size: 4701 Fragments arranged by size: 4701 2824 1419 817 554 552 338 332 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5398 487 Size: 683 56 4172 6700 Fragments arranged by size: 6700 4172 683 56 BtsI GCAGTG_nn' Cuts at: 2765 3228 4830 5072 5733 6022 6424 8481 9127 Size: 463 1602 242 661 289 402 2057 646 Cuts at: 9127 9921 9941 2765 Size: 794 20 4435 Fragments arranged by size: 4435 2057 1602 794 661 646 463 402 289 242 20 ClaI AT'CG_AT Cuts at: 1554 3573 6455 1554 Size: 2019 2882 6710 Fragments arranged by size: 6710 2882 2019 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 8657 8692 529 Size: 35 8093 35 3448 Fragments arranged by size: 8093 3448 35 35 DraI TTT'AAA Cuts at: 2950 3975 7479 7881 8915 2950 Size: 1025 3504 402 1034 5646 Fragments arranged by size: 5646 3504 1034 1025 402 DraIII CAC_nnn'GTG Cuts at: 4870 4872 4969 5140 4870 Size: 2 97 171 11341 Fragments arranged by size: 11341 171 97 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10618 809 Size: 507 9302 1802 Fragments arranged by size: 9302 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2724 3064 3519 Size: 54 1425 50 275 18 894 340 455 Cuts at: 3519 3897 9058 9947 11576 8 Size: 378 5161 889 1629 43 Fragments arranged by size: 5161 1629 1425 894 889 455 378 340 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9058 1537 Size: 275 7246 4090 Fragments arranged by size: 7246 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3098 3223 5003 5440 6741 7396 8008 8259 Size: 1745 125 1780 437 1301 655 612 251 Cuts at: 8259 8298 8349 8571 9541 1353 Size: 39 51 222 970 3423 Fragments arranged by size: 3423 1780 1745 1301 970 655 612 437 251 222 125 51 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5602 10198 10572 10718 387 Size: 1064 138 4013 4596 374 146 1280 Fragments arranged by size: 4596 4013 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3714 3819 4141 4537 8397 3714 Size: 105 322 396 3860 6928 Fragments arranged by size: 6928 3860 396 322 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 4046 4154 4367 4372 4634 4640 Size: 393 402 2012 108 213 5 262 6 Cuts at: 4640 4922 4984 5723 5789 5969 6833 7515 7886 Size: 282 62 739 66 180 864 682 371 Cuts at: 7886 8424 8869 9674 10271 11058 11326 1239 Size: 538 445 805 597 787 268 1524 Fragments arranged by size: 2012 1524 864 805 787 739 682 597 538 445 402 393 371 282 268 262 213 180 108 66 62 6 5 EcoNI CCTnn'n_nnAGG Cuts at: 4589 5096 4589 Size: 507 11104 EcoO109I rG'GnC_Cy Cuts at: 3509 5472 5698 6286 8065 8635 3509 Size: 1963 226 588 1779 570 6485 Fragments arranged by size: 6485 1963 1779 588 570 226 EcoRI G'AATT_C Cuts at: 2421 2457 4282 8920 9298 2421 Size: 36 1825 4638 378 4734 Fragments arranged by size: 4734 4638 1825 378 36 EcoRV GAT'ATC Cuts at: 5898 6655 7892 5898 Size: 757 1237 9617 Fragments arranged by size: 9617 1237 757 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4742 6403 6629 4742 Size: 1661 226 9724 Fragments arranged by size: 9724 1661 226 FspI TGC'GCA Cuts at: 10117 10117 Size: 11611 HaeII r_GCGC'y Cuts at: 1678 4164 10758 1678 Size: 2486 6594 2531 Fragments arranged by size: 6594 2531 2486 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2326 2358 3097 3129 3169 Size: 32 135 32 754 32 739 32 40 Cuts at: 3169 3201 3246 3278 4148 4180 4280 4312 4321 Size: 32 45 32 870 32 100 32 9 Cuts at: 4321 4353 5485 5517 5939 5971 6770 6802 7130 Size: 32 1132 32 422 32 799 32 328 Cuts at: 7130 7162 7447 7479 10252 10284 10326 10358 1373 Size: 32 285 32 2773 32 42 32 2626 Fragments arranged by size: 2773 2626 1132 870 799 754 739 422 328 285 135 100 45 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 2584 2824 3153 3664 4306 5826 Size: 988 281 646 240 329 511 642 1520 Cuts at: 5826 7047 7687 8790 8955 9201 669 Size: 1221 640 1103 165 246 3079 Fragments arranged by size: 3079 1520 1221 1103 988 646 642 640 511 329 281 246 240 165 HindIII A'AGCT_T Cuts at: 748 4178 6845 7875 748 Size: 3430 2667 1030 4484 Fragments arranged by size: 4484 3430 2667 1030 HpaI GTT'AAC Cuts at: 9201 9201 Size: 11611 KasI G'GCGC_C Cuts at: 4160 4160 Size: 11611 KpnI G_GTAC'C Cuts at: 8936 8936 Size: 11611 MfeI C'AATT_G Cuts at: 3042 5781 9210 3042 Size: 2739 3429 5443 Fragments arranged by size: 5443 3429 2739 MluI A'CGCG_T Cuts at: 11487 11487 Size: 11611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3232 4425 7419 10725 10909 1305 Size: 1927 1193 2994 3306 184 2007 Fragments arranged by size: 3306 2994 2007 1927 1193 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2726 3066 3521 3899 10 Size: 54 1425 343 894 340 455 378 7722 Fragments arranged by size: 7722 1425 894 455 378 343 340 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2346 3620 6077 7479 9569 9928 Size: 648 876 304 1274 2457 1402 2090 359 Cuts at: 9928 10087 518 Size: 159 2042 Fragments arranged by size: 2457 2090 2042 1402 1274 876 648 359 304 159 MspA1I CmG'CkG Cuts at: 1606 2755 3551 4189 4641 4748 5004 5468 7354 Size: 1149 796 638 452 107 256 464 1886 Cuts at: 7354 7713 7844 7906 8292 8632 9027 9704 10852 Size: 359 131 62 386 340 395 677 1148 Cuts at: 10852 11097 1606 Size: 245 2120 Fragments arranged by size: 2120 1886 1149 1148 796 677 638 464 452 395 386 359 340 256 245 131 107 62 NarI GG'CG_CC Cuts at: 4161 4161 Size: 11611 NcoI C'CATG_G Cuts at: 513 1065 2484 3301 3855 4525 7423 513 Size: 552 1419 817 554 670 2898 4701 Fragments arranged by size: 4701 2898 1419 817 670 554 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 11611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11611 NotI GC'GGCC_GC Cuts at: 9058 9058 Size: 11611 NsiI A_TGCA'T Cuts at: 7060 7060 Size: 11611 NspI r_CATG'y Cuts at: 1132 1229 3896 4900 7702 8969 10514 1132 Size: 97 2667 1004 2802 1267 1545 2229 Fragments arranged by size: 2802 2667 2229 1545 1267 1004 97 PasI CC'CwG_GG Cuts at: 1348 5239 7133 8069 1348 Size: 3891 1894 936 4890 Fragments arranged by size: 4890 3891 1894 936 PciI A'CATG_T Cuts at: 10510 10510 Size: 11611 PflMI CCAn_nnn'nTGG Cuts at: 1108 2663 4121 5562 1108 Size: 1555 1458 1441 7157 Fragments arranged by size: 7157 1555 1458 1441 PfoI T'CCnGG_A Cuts at: 4388 7036 4388 Size: 2648 8963 PmeI GTTT'AAAC Cuts at: 8915 8915 Size: 11611 PmlI CAC'GTG Cuts at: 4082 4082 Size: 11611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9680 9712 11193 11225 9680 Size: 32 1481 32 10066 Fragments arranged by size: 10066 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5472 5698 6286 8065 8635 5472 Size: 226 588 1779 570 8448 Fragments arranged by size: 8448 1779 588 570 226 PshAI GACnn'nnGTC Cuts at: 1613 6097 1613 Size: 4484 7127 PsiI TTA'TAA Cuts at: 4430 6705 9181 4430 Size: 2275 2476 6860 Fragments arranged by size: 6860 2476 2275 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6174 6206 6174 Size: 32 11579 PstI C_TGCA'G Cuts at: 830 2083 2760 3582 4593 6189 7612 8133 8963 Size: 1253 677 822 1011 1596 1423 521 830 Cuts at: 8963 830 Size: 3478 Fragments arranged by size: 3478 1596 1423 1253 1011 830 822 677 521 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11611 PvuII CAG'CTG Cuts at: 2755 3551 4748 5468 7354 7713 7906 8292 2755 Size: 796 1197 720 1886 359 193 386 6074 Fragments arranged by size: 6074 1886 1197 796 720 386 359 193 SacI G_AGCT'C Cuts at: 3716 3821 4143 4539 8399 3716 Size: 105 322 396 3860 6928 Fragments arranged by size: 6928 3860 396 322 105 SacII CC_GC'GG Cuts at: 4190 4190 Size: 11611 SalI G'TCGA_C Cuts at: 1936 3662 7685 8953 1936 Size: 1726 4023 1268 4594 Fragments arranged by size: 4594 4023 1726 1268 SanDI GG'GwC_CC Cuts at: 5472 8065 5472 Size: 2593 9018 SapI GCTCTTCn'nnn_ Cuts at: 5003 5440 5003 Size: 437 11174 SbfI CC_TGCA'GG Cuts at: 8963 8963 Size: 11611 ScaI AGT'ACT Cuts at: 3460 9859 11571 3460 Size: 6399 1712 3500 Fragments arranged by size: 6399 3500 1712 SfcI C'TryA_G Cuts at: 826 1045 2079 2756 3578 4589 6185 6277 7608 Size: 219 1034 677 822 1011 1596 92 1331 Cuts at: 7608 8129 8530 8959 10094 10775 10966 826 Size: 521 401 429 1135 681 191 1471 Fragments arranged by size: 1596 1471 1331 1135 1034 1011 822 681 677 521 429 401 219 191 92 SfoI GGC'GCC Cuts at: 4162 4162 Size: 11611 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11611 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11611 SmaI CCC'GGG Cuts at: 1872 8938 1872 Size: 7066 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5373 8392 8702 8730 8771 9715 Size: 197 926 3430 3019 310 28 41 944 Cuts at: 9715 10616 10878 820 Size: 901 262 1553 Fragments arranged by size: 3430 3019 1553 944 926 901 310 262 197 41 28 SnaBI TAC'GTA Cuts at: 493 9402 493 Size: 8909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11611 SphI G_CATG'C Cuts at: 1132 7702 8969 1132 Size: 6570 1267 3774 Fragments arranged by size: 6570 3774 1267 SspI AAT'ATT Cuts at: 5 52 2116 8201 8525 9535 5 Size: 47 2064 6085 324 1010 2081 Fragments arranged by size: 6085 2081 2064 1010 324 47 StuI AGG'CCT Cuts at: 1517 4517 1517 Size: 3000 8611 StyI C'CwwG_G Cuts at: 513 1065 2484 3301 3855 4525 4544 6013 7423 Size: 552 1419 817 554 670 19 1469 1410 Cuts at: 7423 8112 8853 9050 513 Size: 689 741 197 3074 Fragments arranged by size: 3074 1469 1419 1410 817 741 689 670 554 552 197 19 SwaI ATTT'AAAT Cuts at: 7479 7479 Size: 11611 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3814 8861 9638 9655 9808 9993 10438 Size: 1255 2234 5047 777 17 153 185 445 Cuts at: 10438 325 Size: 1498 Fragments arranged by size: 5047 2234 1498 1255 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 3396 3458 Size: 274 80 33 51 1443 402 1017 62 Cuts at: 3458 4062 4732 9857 11569 96 Size: 604 670 5125 1712 138 Fragments arranged by size: 5125 1712 1443 1017 670 604 402 274 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2390 2422 8636 8668 11571 11603 2390 Size: 32 6214 32 2903 32 2398 Fragments arranged by size: 6214 2903 2398 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11611 XbaI T'CTAG_A Cuts at: 8947 8947 Size: 11611 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3619 5559 6124 7660 8252 8893 3619 Size: 1940 565 1536 592 641 6337 Fragments arranged by size: 6337 1940 1536 641 592 565 XhoI C'TCGA_G Cuts at: 1943 8730 1943 Size: 6787 4824 XmaI C'CCGG_G Cuts at: 1870 8936 1870 Size: 7066 4545 XmnI GAAnn'nnTTC Cuts at: 2425 4517 7171 7185 9740 2425 Size: 2092 2654 14 2555 4296 Fragments arranged by size: 4296 2654 2555 2092 14 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5496 7690 9419 276 Size: 53 83 186 936 3962 2194 1729 2468 Fragments arranged by size: 3962 2468 2194 1729 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI RsrII SexAI SfiI SrfI