Restriction map for
pFN21AA1516
(Linear) (Six-base) MAPSORT of: KIAA1516.seq Check: 599 from: 1 to: 6906
KIAA1516.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 6702 6906
Size: 6702 204
AatII G_ACGT'C
Cuts at: 0 3493 5687 6906
Size: 3493 2194 1219
Fragments arranged by size:
3493 2194 1219
AccI GT'mk_AC
Cuts at: 0 1021 1658 2831 5681 6906
Size: 1021 637 1173 2850 1225
Fragments arranged by size:
2850 1225 1173 1021 637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 2041 2149 2635 2917 2979 3718 3784
Size: 29 2012 108 486 282 62 739 66
Cuts at: 3784 3964 4828 5881 6906
Size: 180 864 1053 1025
Fragments arranged by size:
2012 1053 1025 864 739 486 282 180
108 66 62 29
AflII C'TTAA_G
Cuts at: 0 6697 6906
Size: 6697 209
AflIII A'CryG_T
Cuts at: 0 2074 6906
Size: 2074 4832
AgeI A'CCGG_T
Cuts at: 0 1399 6906
Size: 1399 5507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1666 3744 6906
Size: 1666 2078 3162
Fragments arranged by size:
3162 2078 1666
AlwNI CAG_nnn'CTG
Cuts at: 0 25 292 2580 2908 3066 5325 5352 5772
Size: 25 267 2288 328 158 2259 27 420
Cuts at: 5772 6123 6906
Size: 351 783
Fragments arranged by size:
2288 2259 783 420 351 328 267 158
27 25
ApaLI G'TGCA_C
Cuts at: 0 2569 6906
Size: 2569 4337
ApoI r'AATT_y
Cuts at: 0 416 452 2277 2755 3040 3649 4134 4496
Size: 416 36 1825 478 285 609 485 362
Cuts at: 4496 4534 4942 5026 5970 6131 6275 6688 6906
Size: 38 408 84 944 161 144 413 218
Fragments arranged by size:
1825 944 609 485 478 416 413 408
362 285 218 161 144 84 38 36
AseI AT'TA_AT
Cuts at: 0 3377 6095 6906
Size: 3377 2718 811
Fragments arranged by size:
3377 2718 811
AvaI C'yCGr_G
Cuts at: 0 6725 6797 6906
Size: 6725 72 109
Fragments arranged by size:
6725 109 72
BamHI G'GATC_C
Cuts at: 0 1465 5598 6906
Size: 1465 4133 1308
Fragments arranged by size:
4133 1465 1308
BanI G'GyrC_C
Cuts at: 0 2155 2967 6103 6906
Size: 2155 812 3136 803
Fragments arranged by size:
3136 2155 812 803
BanII G_rGCy'C
Cuts at: 0 1711 1816 2138 2534 3447 3547 5128 6077
Size: 1711 105 322 396 913 100 1581 949
Cuts at: 6077 6394 6906
Size: 317 512
Fragments arranged by size:
1711 1581 949 913 512 396 322 317
105 100
BbeI G_GCGC'C
Cuts at: 0 2159 6906
Size: 2159 4747
BbsI GAAGACnn'nnnn_
Cuts at: 0 401 941 3521 4814 6607 6906
Size: 401 540 2580 1293 1793 299
Fragments arranged by size:
2580 1793 1293 540 401 299
BbvCI CC'TCA_GC
Cuts at: 0 389 4039 4475 6906
Size: 389 3650 436 2431
Fragments arranged by size:
3650 2431 436 389
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4279 4313 5661 5695 6706 6735 6740 6769
Size: 4279 34 1348 34 1011 29 5 29
Cuts at: 6769 6906
Size: 137
Fragments arranged by size:
4279 1348 1011 137 34 34 29 29
5
BciVI GTATCCnnnnn_n'
Cuts at: 0 1211 5417 6298 6850 6906
Size: 1211 4206 881 552 56
Fragments arranged by size:
4206 1211 881 552 56
BclI T'GATC_A
Cuts at: 0 512 759 4953 6906
Size: 512 247 4194 1953
Fragments arranged by size:
4194 1953 512 247
BfrBI ATG'CAT
Cuts at: 0 5053 6906
Size: 5053 1853
BglI GCCn_nnn'nGGC
Cuts at: 0 70 1502 2846 6906
Size: 70 1432 1344 4060
Fragments arranged by size:
4060 1432 1344 70
BglII A'GATC_T
Cuts at: 0 1785 3813 5583 5793 6906
Size: 1785 2028 1770 210 1113
Fragments arranged by size:
2028 1785 1770 1113 210
BlpI GC'TnA_GC
Cuts at: 0 2744 6906
Size: 2744 4162
Bme1580I G_kGCm'C
Cuts at: 0 1693 2082 2573 3728 5647 6906
Size: 1693 389 491 1155 1919 1259
Fragments arranged by size:
1919 1693 1259 1155 491 389
BmgBI CAC'GTC
Cuts at: 0 1424 5228 6906
Size: 1424 3804 1678
Fragments arranged by size:
3804 1678 1424
BmrI ACTGGGnnnn_n'
Cuts at: 0 60 1188 2360 2776 2893 3944 4942 5642
Size: 60 1128 1172 416 117 1051 998 700
Cuts at: 5642 6906
Size: 1264
Fragments arranged by size:
1264 1172 1128 1051 998 700 416 117
60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1830 3968 6906
Size: 1830 2138 2938
Fragments arranged by size:
2938 2138 1830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2362 2367 2629 5510 6419 6864 6906
Size: 2362 5 262 2881 909 445 42
Fragments arranged by size:
2881 2362 909 445 262 42 5
Bpu10I CC'TnA_GC
Cuts at: 0 389 4039 4475 6535 6906
Size: 389 3650 436 2060 371
Fragments arranged by size:
3650 2060 436 389 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3353 6408 6751 6906
Size: 3353 3055 343 155
Fragments arranged by size:
3353 3055 343 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 102 1999 2170 6906
Size: 102 1897 171 4736
Fragments arranged by size:
4736 1897 171 102
BsaAI yAC'GTr
Cuts at: 0 2077 2547 6906
Size: 2077 470 4359
Fragments arranged by size:
4359 2077 470
BsaBI GATnn'nnATC
Cuts at: 0 4342 6906
Size: 4342 2564
BsaHI Gr'CG_yC
Cuts at: 0 2156 3490 4639 5684 6906
Size: 2156 1334 1149 1045 1222
Fragments arranged by size:
2156 1334 1222 1149 1045
BsaWI w'CCGG_w
Cuts at: 0 1399 2161 2321 6906
Size: 1399 762 160 4585
Fragments arranged by size:
4585 1399 762 160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 972 1002 1164 1194 2143 2173 2275 2305
Size: 972 30 162 30 949 30 102 30
Cuts at: 2305 3480 3510 3552 3582 6637 6667 6906
Size: 1175 30 42 30 3055 30 239
Fragments arranged by size:
3055 1175 972 949 239 162 102 42
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 1870 2028 2368 4768 6277 6412 6415
Size: 682 1188 158 340 2400 1509 135 3
Cuts at: 6415 6868 6906
Size: 453 38
Fragments arranged by size:
2400 1509 1188 682 453 340 158 135
38 3
BseYI C'CCAG_C
Cuts at: 0 1278 1546 2036 2700 3175 3463 4319 5330
Size: 1278 268 490 664 475 288 856 1011
Cuts at: 5330 6332 6906
Size: 1002 574
Fragments arranged by size:
1278 1011 1002 856 664 574 490 475
288 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2068 4005 4711 6464 6701 6736 6906
Size: 2068 1937 706 1753 237 35 170
Fragments arranged by size:
2068 1937 1753 706 237 170 35
BsiHKAI G_wGCw'C
Cuts at: 0 534 1711 1772 1816 2138 2534 2573 3168
Size: 534 1177 61 44 322 396 39 595
Cuts at: 3168 3949 6394 6906
Size: 781 2445 512
Fragments arranged by size:
2445 1177 781 595 534 512 396 322
61 44 39
BsmI GAATG_Cn'
Cuts at: 0 1705 3204 3408 4716 5180 6906
Size: 1705 1499 204 1308 464 1726
Fragments arranged by size:
1726 1705 1499 1308 464 204
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4322 6906
Size: 4322 2584
Bsp1286I G_dGCh'C
Cuts at: 0 534 1693 1711 1772 1816 2082 2138 2534
Size: 534 1159 18 61 44 266 56 396
Cuts at: 2534 2573 3168 3447 3547 3728 3949 5128 5647
Size: 39 595 279 100 181 221 1179 519
Cuts at: 5647 6077 6394 6906
Size: 430 317 512
Fragments arranged by size:
1179 1159 595 534 519 512 430 396
317 279 266 221 181 100 61 56
44 39 18
BspEI T'CCGG_A
Cuts at: 0 2321 6906
Size: 2321 4585
BspHI T'CATG_A
Cuts at: 0 2168 3272 6906
Size: 2168 1104 3634
Fragments arranged by size:
3634 2168 1104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4442 4734 5300 6702 6906
Size: 4442 292 566 1402 204
Fragments arranged by size:
4442 1402 566 292 204
BsrDI GCAATG_nn'
Cuts at: 0 1336 2917 3902 4468 5489 6906
Size: 1336 1581 985 566 1021 1417
Fragments arranged by size:
1581 1417 1336 1021 985 566
BsrFI r'CCGG_y
Cuts at: 0 1399 6906
Size: 1399 5507
BsrGI T'GTAC_A
Cuts at: 0 374 6906
Size: 374 6532
BssSI C'ACGA_G
Cuts at: 0 3997 6906
Size: 3997 2909
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3066 3199 6123 6906
Size: 3066 133 2924 783
Fragments arranged by size:
3066 2924 783 133
BstBI TT'CG_AA
Cuts at: 0 5789 6906
Size: 5789 1117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 343 1428 5534 6906
Size: 343 1085 4106 1372
Fragments arranged by size:
4106 1372 1085 343
BstYI r'GATC_y
Cuts at: 0 1249 1465 1785 2808 3702 3813 4376 5583
Size: 1249 216 320 1023 894 111 563 1207
Cuts at: 5583 5598 5793 6906
Size: 15 195 1113
Fragments arranged by size:
1249 1207 1113 1023 894 563 320 216
195 111 15
BstZ17I GTA'TAC
Cuts at: 0 1022 2832 6906
Size: 1022 1810 4074
Fragments arranged by size:
4074 1810 1022
Bsu36I CC'TnA_GG
Cuts at: 0 1951 6906
Size: 1951 4955
BtgI C'CryG_G
Cuts at: 0 479 1296 1850 2182 2520 2594 5418 6906
Size: 479 817 554 332 338 74 2824 1488
Fragments arranged by size:
2824 1488 817 554 479 338 332 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3393 6906
Size: 3393 3513
BtsI GCAGTG_nn'
Cuts at: 0 760 1223 2825 3067 3728 4017 4419 6476
Size: 760 463 1602 242 661 289 402 2057
Cuts at: 6476 6906
Size: 430
Fragments arranged by size:
2057 1602 760 661 463 430 402 289
242
ClaI AT'CG_AT
Cuts at: 0 1568 4450 6906
Size: 1568 2882 2456
Fragments arranged by size:
2882 2456 1568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6652 6687 6906
Size: 6652 35 219
Fragments arranged by size:
6652 219 35
DraI TTT'AAA
Cuts at: 0 945 1970 5474 5876 6906
Size: 945 1025 3504 402 1030
Fragments arranged by size:
3504 1030 1025 945 402
DraIII CAC_nnn'GTG
Cuts at: 0 2865 2867 2964 3135 6906
Size: 2865 2 97 171 3771
Fragments arranged by size:
3771 2865 171 97 2
EaeI y'GGCC_r
Cuts at: 0 719 1059 1514 1892 6906
Size: 719 340 455 378 5014
Fragments arranged by size:
5014 719 455 378 340
EarI CTCTTCn'nnn_
Cuts at: 0 1093 1218 2998 3435 4736 5391 6003 6254
Size: 1093 125 1780 437 1301 655 612 251
Cuts at: 6254 6293 6344 6566 6906
Size: 39 51 222 340
Fragments arranged by size:
1780 1301 1093 655 612 437 340 251
222 125 51 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3597 6906
Size: 3597 3309
EcoICRI GAG'CTC
Cuts at: 0 1709 1814 2136 2532 6392 6906
Size: 1709 105 322 396 3860 514
Fragments arranged by size:
3860 1709 514 396 322 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 2041 2149 2362 2367 2629 2635 2917
Size: 29 2012 108 213 5 262 6 282
Cuts at: 2917 2979 3718 3784 3964 4828 5510 5881 6419
Size: 62 739 66 180 864 682 371 538
Cuts at: 6419 6864 6906
Size: 445 42
Fragments arranged by size:
2012 864 739 682 538 445 371 282
262 213 180 108 66 62 42 29
6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2584 3091 6906
Size: 2584 507 3815
Fragments arranged by size:
3815 2584 507
EcoO109I rG'GnC_Cy
Cuts at: 0 1504 3467 3693 4281 6060 6630 6906
Size: 1504 1963 226 588 1779 570 276
Fragments arranged by size:
1963 1779 1504 588 570 276 226
EcoRI G'AATT_C
Cuts at: 0 416 452 2277 6906
Size: 416 36 1825 4629
Fragments arranged by size:
4629 1825 416 36
EcoRV GAT'ATC
Cuts at: 0 3893 4650 5887 6906
Size: 3893 757 1237 1019
Fragments arranged by size:
3893 1237 1019 757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2737 4398 4624 6906
Size: 2737 1661 226 2282
Fragments arranged by size:
2737 2282 1661 226
HaeII r_GCGC'y
Cuts at: 0 2159 6906
Size: 2159 4747
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 321 353 1092 1124 1164 1196 1241 1273
Size: 321 32 739 32 40 32 45 32
Cuts at: 1273 2143 2175 2275 2307 2316 2348 3480 3512
Size: 870 32 100 32 9 32 1132 32
Cuts at: 3512 3934 3966 4765 4797 5125 5157 5442 5474
Size: 422 32 799 32 328 32 285 32
Cuts at: 5474 6906
Size: 1432
Fragments arranged by size:
1432 1132 870 799 739 422 328 321
285 100 45 40 32 32 32 32
32 32 32 32 32 32 32 32
9
HincII GTy'rAC
Cuts at: 0 579 819 1148 1659 2301 3821 5042 5682
Size: 579 240 329 511 642 1520 1221 640
Cuts at: 5682 6785 6906
Size: 1103 121
Fragments arranged by size:
1520 1221 1103 642 640 579 511 329
240 121
HindIII A'AGCT_T
Cuts at: 0 2173 4840 5870 6906
Size: 2173 2667 1030 1036
Fragments arranged by size:
2667 2173 1036 1030
KasI G'GCGC_C
Cuts at: 0 2155 6906
Size: 2155 4751
MfeI C'AATT_G
Cuts at: 0 1037 3776 6906
Size: 1037 2739 3130
Fragments arranged by size:
3130 2739 1037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1227 2420 5414 6906
Size: 1227 1193 2994 1492
Fragments arranged by size:
2994 1492 1227 1193
MscI TGG'CCA
Cuts at: 0 721 1061 1516 1894 6906
Size: 721 340 455 378 5012
Fragments arranged by size:
5012 721 455 378 340
MslI CAynn'nnrTG
Cuts at: 0 37 341 1615 4072 5474 6906
Size: 37 304 1274 2457 1402 1432
Fragments arranged by size:
2457 1432 1402 1274 304 37
MspA1I CmG'CkG
Cuts at: 0 750 1546 2184 2636 2743 2999 3463 5349
Size: 750 796 638 452 107 256 464 1886
Cuts at: 5349 5708 5839 5901 6287 6627 6906
Size: 359 131 62 386 340 279
Fragments arranged by size:
1886 796 750 638 464 452 386 359
340 279 256 131 107 62
NarI GG'CG_CC
Cuts at: 0 2156 6906
Size: 2156 4750
NcoI C'CATG_G
Cuts at: 0 479 1296 1850 2520 5418 6906
Size: 479 817 554 670 2898 1488
Fragments arranged by size:
2898 1488 817 670 554 479
NsiI A_TGCA'T
Cuts at: 0 5055 6906
Size: 5055 1851
NspI r_CATG'y
Cuts at: 0 1891 2895 5697 6906
Size: 1891 1004 2802 1209
Fragments arranged by size:
2802 1891 1209 1004
PasI CC'CwG_GG
Cuts at: 0 3234 5128 6064 6906
Size: 3234 1894 936 842
Fragments arranged by size:
3234 1894 936 842
PflMI CCAn_nnn'nTGG
Cuts at: 0 658 2116 3557 6906
Size: 658 1458 1441 3349
Fragments arranged by size:
3349 1458 1441 658
PfoI T'CCnGG_A
Cuts at: 0 2383 5031 6906
Size: 2383 2648 1875
Fragments arranged by size:
2648 2383 1875
PmlI CAC'GTG
Cuts at: 0 2077 6906
Size: 2077 4829
PpuMI rG'GwC_Cy
Cuts at: 0 3467 3693 4281 6060 6630 6906
Size: 3467 226 588 1779 570 276
Fragments arranged by size:
3467 1779 588 570 276 226
PshAI GACnn'nnGTC
Cuts at: 0 4092 6906
Size: 4092 2814
PsiI TTA'TAA
Cuts at: 0 2425 4700 6906
Size: 2425 2275 2206
Fragments arranged by size:
2425 2275 2206
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4169 4201 6906
Size: 4169 32 2705
Fragments arranged by size:
4169 2705 32
PstI C_TGCA'G
Cuts at: 0 78 755 1577 2588 4184 5607 6128 6906
Size: 78 677 822 1011 1596 1423 521 778
Fragments arranged by size:
1596 1423 1011 822 778 677 521 78
PvuII CAG'CTG
Cuts at: 0 750 1546 2743 3463 5349 5708 5901 6287
Size: 750 796 1197 720 1886 359 193 386
Cuts at: 6287 6906
Size: 619
Fragments arranged by size:
1886 1197 796 750 720 619 386 359
193
SacI G_AGCT'C
Cuts at: 0 1711 1816 2138 2534 6394 6906
Size: 1711 105 322 396 3860 512
Fragments arranged by size:
3860 1711 512 396 322 105
SacII CC_GC'GG
Cuts at: 0 2185 6906
Size: 2185 4721
SalI G'TCGA_C
Cuts at: 0 1657 5680 6906
Size: 1657 4023 1226
Fragments arranged by size:
4023 1657 1226
SanDI GG'GwC_CC
Cuts at: 0 3467 6060 6906
Size: 3467 2593 846
Fragments arranged by size:
3467 2593 846
SapI GCTCTTCn'nnn_
Cuts at: 0 2998 3435 6906
Size: 2998 437 3471
Fragments arranged by size:
3471 2998 437
ScaI AGT'ACT
Cuts at: 0 1455 6906
Size: 1455 5451
SfcI C'TryA_G
Cuts at: 0 74 751 1573 2584 4180 4272 5603 6124
Size: 74 677 822 1011 1596 92 1331 521
Cuts at: 6124 6525 6906
Size: 401 381
Fragments arranged by size:
1596 1331 1011 822 677 521 401 381
92 74
SfoI GGC'GCC
Cuts at: 0 2157 6906
Size: 2157 4749
SmlI C'TyrA_G
Cuts at: 0 3368 6387 6697 6725 6766 6906
Size: 3368 3019 310 28 41 140
Fragments arranged by size:
3368 3019 310 140 41 28
SphI G_CATG'C
Cuts at: 0 5697 6906
Size: 5697 1209
SspI AAT'ATT
Cuts at: 0 111 6196 6520 6906
Size: 111 6085 324 386
Fragments arranged by size:
6085 386 324 111
StuI AGG'CCT
Cuts at: 0 2512 6906
Size: 2512 4394
StyI C'CwwG_G
Cuts at: 0 479 1296 1850 2520 2539 4008 5418 6107
Size: 479 817 554 670 19 1469 1410 689
Cuts at: 6107 6848 6906
Size: 741 58
Fragments arranged by size:
1469 1410 817 741 689 670 554 479
58 19
SwaI ATTT'AAAT
Cuts at: 0 5474 6906
Size: 5474 1432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1809 6856 6906
Size: 1809 5047 50
Fragments arranged by size:
5047 1809 50
TatI w'GTAC_w
Cuts at: 0 374 1391 1453 2057 2727 6906
Size: 374 1017 62 604 670 4179
Fragments arranged by size:
4179 1017 670 604 374 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 385 417 6631 6663 6906
Size: 385 32 6214 32 243
Fragments arranged by size:
6214 385 243 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1614 3554 4119 5655 6247 6888 6906
Size: 1614 1940 565 1536 592 641 18
Fragments arranged by size:
1940 1614 1536 641 592 565 18
XhoI C'TCGA_G
Cuts at: 0 6725 6906
Size: 6725 181
XmnI GAAnn'nnTTC
Cuts at: 0 420 2512 5166 5180 6906
Size: 420 2092 2654 14 1726
Fragments arranged by size:
2654 2092 1726 420 14
ZraI GAC'GTC
Cuts at: 0 3491 5685 6906
Size: 3491 2194 1221
Fragments arranged by size:
3491 2194 1221
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MscI MslI MspA1I NarI NcoI
NsiI NspI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PsrI PstI PvuII SacI SacII SalI SanDI
SapI ScaI SfcI SfoI SmlI SphI SspI StuI
StyI SwaI TaqII TatI TstI XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AhdI AleI AloI ApaI AscI
AsiSI AvrII BaeI BmtI BsiEI BsiWI BsrBI BssHII
BstEII DrdI EagI FseI FspI FspAI HpaI KpnI
MluI NaeI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PpiI PspOMI PspXI PvuI RsrII SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
Tth111I XbaI XmaI