HUGE

Gene/Protein Characteristic Table for KIAA1619

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK02043
Accession No. :	AB046839
Description :	Semaphorin-4G precursor.
HUGO Gene Name :	sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G)
Clone Name :	fj14720 [Vector Info]
Flexi ORF Clone :	pF1KA1619
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 4094 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1484 bp

Genome contig ID gi89161187f_102622582
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
CCTGCCGTTGGTTAATAAACACCCTTTTTCCCCAC
Flanking genome sequence
(112782 - 112831)
----+----*----+----*----+----*----+----*----+----*
AGCTATGACTAGTCTGCTTCATCTAGATGCGCCCACCCCTGATCACCAGG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 10 f 102722582 102735362 14 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 869 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAI10916	100.0	sema domain, im...	Homo sapiens
Q9NTN9	99.4	Semaphorin-4G; ...	Homo sapiens
XP_001109124	98.7	sema domain, im...	Macaca mulatta
XP_001109068	98.1	semaphorin 4G i...	Macaca mulatta
XP_001916730	93.7	sema domain, im...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051526	2.8e-53	41.5	KIAA1739
AB051532	1.5e-40	38.4	KIAA1745
AB002329	8.8e-21	31.5	KIAA0331
AB037789	8.6e-11	32.9	KIAA1368
AB040878	1e-10	29.5	KIAA1445

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR001627 82 515 PF01403 Semaphorin/CD100 antigen

IPR002165 533 589 PF01437 Plexin

HMMSmart IPR001627 82 515 SM00630 Semaphorin/CD100 antigen

IPR003659 533 589 SM00423 Plexin/semaphorin/integrin

IPR003599 600 682 SM00409 Immunoglobulin subtype

ProfileScan IPR001627 61 531 PS51004 Semaphorin/CD100 antigen

IPR007110 598 680 PS50835 Immunoglobulin-like

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	82	515	PF01403	Semaphorin/CD100 antigen
IPR002165	533	589	PF01437	Plexin
HMMSmart	IPR001627	82	515	SM00630	Semaphorin/CD100 antigen
IPR003659	533	589	SM00423	Plexin/semaphorin/integrin
IPR003599	600	682	SM00409	Immunoglobulin subtype
ProfileScan	IPR001627	61	531	PS51004	Semaphorin/CD100 antigen
IPR007110	598	680	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 5 PPPQPVTLAPFGGLVSLGLP 24 SECONDARY 20

2 28 WGRLWPLLLSILTATAVPGPSL 49 SECONDARY 22

3 707 LYVLAIAALGGLCLILASSLLYV 729 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	PPPQPVTLAPFGGLVSLGLP	24	SECONDARY	20
2	28	WGRLWPLLLSILTATAVPGPSL	49	SECONDARY	22
3	707	LYVLAIAALGGLCLILASSLLYV	729	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GTAGCTGAGAACGTGGAATCC

Primer_r
: TGGTTGTAAGTGGGGGCATTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 10

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	1484 bp
Genome contig ID	gi89161187f_102622582
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- CCTGCCGTTGGTTAATAAACACCCTTTTTCCCCAC
Flanking genome sequence (112782 - 112831)	----+--------+--------+--------+--------+----* AGCTATGACTAGTCTGCTTCATCTAGATGCGCCCACCCCTGATCACCAGG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	10	f	102722582	102735362	14	99.4	Perfect prediction

Primer_f	: GTAGCTGAGAACGTGGAATCC
Primer_r	: TGGTTGTAAGTGGGGGCATTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1619

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information