HUGE

Gene/Protein Characteristic Table for KIAA1533

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB040966
Description :	GRAM domain-containing protein 1A.
HUGO Gene Name :
Clone Name :	fh00910 [Vector Info]
Flexi ORF Clone :	pF1KA1533
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 5246 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
YES YES

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 223 bp

Genome contig ID gi42406306f_40092632
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
GGGGCCGACAGTGCCCCAATAAAGGGTCAGAAGTG
Flanking genome sequence
(116583 - 116632)
----+----*----+----*----+----*----+----*----+----*
GCTGTGGCTGTGCCTGTGGGGCCCTGGGAGTGGGTGGGAGCCTCCTGTTG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 19 f 40192163 40209213 18 99.8 Terminal No-hit

Features of the protein sequence	Description

Length: 651 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q96CP6	99.5	GRAM domain-con...	Homo sapiens
BAG10472	99.8	GRAM domain-con...	synthetic construct
BAG53435	99.5	unnamed protein...	Homo sapiens
XP_001157243	99.1	GRAM domain con...	Pan troglodytes
XP_524216	99.1	GRAM domain con...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB033027	3.1e-67	47.3	KIAA1201
AB020689	0.0002	34.8	KIAA0882

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR004182 21 88 PF02893 GRAM

HMMSmart IPR004182 21 88 SM00568 GRAM

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004182	21	88	PF02893	GRAM
HMMSmart	IPR004182	21	88	SM00568	GRAM

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 534 IPSALVLISIVSLIILIALNVLL 556 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	534	IPSALVLISIVSLIILIALNVLL	556	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TGCTGCTATCATCACTTACTG

Primer_r
: CTTTACAGAGCATACGGAGTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 19

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	YES	YES
Warning for coding interruption:	YES	NO

Length of 3'UTR	223 bp
Genome contig ID	gi42406306f_40092632
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- GGGGCCGACAGTGCCCCAATAAAGGGTCAGAAGTG
Flanking genome sequence (116583 - 116632)	----+--------+--------+--------+--------+----* GCTGTGGCTGTGCCTGTGGGGCCCTGGGAGTGGGTGGGAGCCTCCTGTTG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	19	f	40192163	40209213	18	99.8	Terminal No-hit

Primer_f	: TGCTGCTATCATCACTTACTG
Primer_r	: CTTTACAGAGCATACGGAGTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1533

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information