##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp718I AviII BbrPI
BcgI BciVI BclI BsaAI BsiWI
Bsp1407I BspDI BspHI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII KpnI
KspI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2309 GACGTC GACGT^C
AccIII 68 TCCGGA T^CCGGA
AcsI 972 GAATTT [GA]^AATT[CT]
AflII 3599 CTTAAG C^TTAAG
AflIII 244 ACATGT A^C[GA][CT]GT
AhdI 2097 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 972 GAATTT [GA]^AATT[CT]
AspEI 2097 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4559 CCTAGG C^CTAGG
BamHI 4162 GGATCC G^GATCC
BbeI 5154 GGCGCC GGCGC^C
BfrI 3599 CTTAAG C^TTAAG
BglII 293 AGATCT A^GATCT
BlnI 4559 CCTAGG C^CTAGG
BsaBI 219 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 68 TCCGGA [AT]^CCGG[AT]
BseAI 68 TCCGGA T^CCGGA
BsmBI 1645 CGTCTC CGTCTC(1/5)
BspEI 68 TCCGGA T^CCGGA
BspLU11I 244 ACATGT A^CATGT
BstEII 1446 GGTGACC G^GT[ACGT]ACC
BstPI 1446 GGTGACC G^GT[ACGT]ACC
DrdI 2348 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2764 CGGCCG C^GGCCG
Eam1105I 2097 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2764 CGGCCG C^GGCCG
Eco52I 2764 CGGCCG C^GGCCG
EcoO65I 1446 GGTGACC G^GT[ACGT]ACC
HpaI 1292 GTTAAC GTT^AAC
KasI 5154 GGCGCC G^GCGCC
MamI 219 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 68 TCCGGA T^CCGGA
NarI 5154 GGCGCC GG^CGCC
PshAI 4687 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4118 CCTGCAGG CCTGCA^GG
ScaI 989 AGTACT AGT^ACT
SfiI 3527 GGCCACACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5154 GGCGCC GGC^GCC
Sse8387I 4118 CCTGCAGG CCTGCA^GG
StuI 3535 AGGCCT AGG^CCT
TaqI 1613 TCGA T^CGA
TthHB8I 1613 TCGA T^CGA
XbaI 4661 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AocI 867 CCTAAGG CC^T[ACGT]AGG
5019 CCTGAGG
AspI 1433 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2176 GACTATGTC
BsiEI 2258 CGGTCG CG[GA][CT]^CG
2764 CGGCCG
BspMI 3543 ACCTGC ACCTGC(4/8)
2554 GCAGGT
BsrDI 3643 GCAATG GCAATG(2/0)
4604 GCAATG
BssSI 1598 CACGAG CACGAG(-5/-1)
3205 CTCGTG
Bsu36I 867 CCTAAGG CC^T[ACGT]AGG
5019 CCTGAGG
Eco81I 867 CCTAAGG CC^T[ACGT]AGG
5019 CCTGAGG
HincII 1251 GTTGAC GT[CT]^[GA]AC
1292 GTTAAC
HindII 1251 GTTGAC GT[CT]^[GA]AC
1292 GTTAAC
PflFI 1433 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2176 GACTATGTC
SexAI 1255 ACCAGGT A^CC[AT]GGT
2123 ACCTGGT
Tth111I 1433 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2176 GACTATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII XmaCI XmaI XmnI