HUGE

Gene/Protein Characteristic Table for KIAA0520

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK01670
Accession No. :	AB011092
Description :	Adenylate cyclase type 9.
HUGO Gene Name :
Clone Name :	hg02989s1 [Vector Info]
Flexi ORF Clone :	pF1KA0520
Source :	Human adult brain
Note :	We replaced hg01313 and hg02989, former representative clones for KIAA0520 with hg02989s1. (2002/5/10,2005/08/06)

Features of the cloned DNA sequence	Description

Length: 7544 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3102 bp

Genome contig ID gi51511732r_3852654
PolyA signal sequence
(ATTAAA,-24)
+----*----+----*----+----*----+----
AAGAAATGGACATTAAAATATTCTAAAATTTAAAT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATATGATGCCTGGTGTTTTCTTATTGAAAAGATGAGCACTGCTTCAGAGA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 16 r 3952654 4106029 12 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 1364 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAI51230	100.0	ADCY9 protein [...	Homo sapiens
O60503	99.9	Adenylate cycla...	Homo sapiens
AAY27880	99.9	adenylyl cyclas...	Homo sapiens
CAB65084	99.8	adenylyl cyclas...	Homo sapiens
XP_510775	97.8	adenylate cycla...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB007882	1.7e-35	28.9	KIAA0422
AB011083	5.7e-33	31.2	KIAA0511
D25538	5.1e-32	30.2	KIAA0037
AB028983	1.9e-29	32.3	KIAA1060

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR001054 396 584 PF00211 Adenylyl cyclase class-3/4/guanylyl cyclase

IPR001054 1060 1255 PF00211 Adenylyl cyclase class-3/4/guanylyl cyclase

HMMSmart IPR001054 336 558 SM00044 Adenylyl cyclase class-3/4/guanylyl cyclase

IPR001054 1034 1238 SM00044 Adenylyl cyclase class-3/4/guanylyl cyclase

ProfileScan IPR001054 405 532 PS50125 Adenylyl cyclase class-3/4/guanylyl cyclase

IPR001054 1069 1209 PS50125 Adenylyl cyclase class-3/4/guanylyl cyclase

ScanRegExp IPR001054 509 532 PS00452 Adenylyl cyclase class-3/4/guanylyl cyclase

IPR001054 1186 1209 PS00452 Adenylyl cyclase class-3/4/guanylyl cyclase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001054	396	584	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
IPR001054	1060	1255	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	336	558	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
IPR001054	1034	1238	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	405	532	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
IPR001054	1069	1209	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	509	532	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase
IPR001054	1186	1209	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 128 RYALFYIGFACLLWSIYFAVHMR 150 PRIMARY 23

2 154 IVMVAPALCFLLVCVGFFLFTFT 176 PRIMARY 23

3 187 SLALTLLVFALTLAAQFQVLTPV 209 SECONDARY 23

4 242 LFLLYTVMHLPLYLSLCLGVAYS 264 PRIMARY 23

5 295 RGLLHGCIHAIGVHLFVMSQVR 316 SECONDARY 22

6 796 FSSLLDVFLSTTVFLTLSTTCFL 818 SECONDARY 23

7 840 LLEVLSLAVSIRMVFFLEDVMAC 862 PRIMARY 23

8 876 RHCIGAILVSLPALAVYSHVTSE 898 PRIMARY 23

9 902 NIHFPVFTGSAALIAVVHYCNFC 924 SECONDARY 23

10 930 MRSSLATVVGAGPLLLLYVSLCP 952 PRIMARY 23

11 986 ASLIGQEVVLVFFLLLLLVWFLN 1008 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	128	RYALFYIGFACLLWSIYFAVHMR	150	PRIMARY	23
2	154	IVMVAPALCFLLVCVGFFLFTFT	176	PRIMARY	23
3	187	SLALTLLVFALTLAAQFQVLTPV	209	SECONDARY	23
4	242	LFLLYTVMHLPLYLSLCLGVAYS	264	PRIMARY	23
5	295	RGLLHGCIHAIGVHLFVMSQVR	316	SECONDARY	22
6	796	FSSLLDVFLSTTVFLTLSTTCFL	818	SECONDARY	23
7	840	LLEVLSLAVSIRMVFFLEDVMAC	862	PRIMARY	23
8	876	RHCIGAILVSLPALAVYSHVTSE	898	PRIMARY	23
9	902	NIHFPVFTGSAALIAVVHYCNFC	924	SECONDARY	23
10	930	MRSSLATVVGAGPLLLLYVSLCP	952	PRIMARY	23
11	986	ASLIGQEVVLVFFLLLLLVWFLN	1008	PRIMARY	23

Expression profile	Description

: RT-PCR Description

Experimental conditions

Primer_f
: ACAGTCGGTTCTTGCTAATTC

Primer_r
: TGCTTCCTGTCACATGGGCTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR	Description

RH mapping information	Description

Chromosome No.

: 16

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: ACAGTCGGTTCTTGCTAATTC
Primer_r	: TGCTTCCTGTCACATGGGCTG
PCR product length	: 157 bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	3102 bp
Genome contig ID	gi51511732r_3852654
PolyA signal sequence (ATTAAA,-24)	+--------+--------+----*----+---- AAGAAATGGACATTAAAATATTCTAAAATTTAAAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATATGATGCCTGGTGTTTTCTTATTGAAAAGATGAGCACTGCTTCAGAGA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	16	r	3952654	4106029	12	99.2	Perfect prediction

Gene/Protein Characteristic Table for KIAA0520

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information