HUGE

Gene/Protein Characteristic Table for KIAA0422

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00533
Accession No. :	AB007882
Description :	Adenylate cyclase type 6.
HUGO Gene Name :
Clone Name :	hh01205s1 [Vector Info]
Flexi ORF Clone :	pF1KSDA0422
Source :	Human adult brain
Note :	We replaced hh01205, former representative clones for KIAA0422 with hh01205s1. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 5877 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 2393 bp

Genome contig ID gi89161190r_47346248
PolyA signal sequence
(AATAAA,-20)
+----*----+----*----+----*----+----
ATATATTTAAATATCAATAAAATCAAACTCTTTGT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAATTCTCTGTTCTCTGTAGCTGTTCTGGGCCCTGGGTGGGTCAGAAGT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 12 r 47446248 47469087 21 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 1160 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW58001	100.0	adenylate cycla...	Homo sapiens
XP_001102392	99.2	similar to aden...	Macaca mulatta
XP_863292	96.6	similar to aden...	Canis lupus fam...
XP_587001	95.5	adenylate cycla...	Bos taurus
O43306	95.5	Adenylate cycla...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB011083	7.7e-46	40.4	KIAA0511
AB028983	2.7e-37	42.4	KIAA1060
D25538	5.3e-37	39.1	KIAA0037
AB011092	5.3e-28	28.9	KIAA0520

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR001054 415 599 PF00211 Adenylyl cyclase class-3/4/guanylyl cyclase

IPR009398 605 714 PF06327 Adenylate cyclase-like

IPR001054 962 1156 PF00211 Adenylyl cyclase class-3/4/guanylyl cyclase

HMMSmart IPR001054 378 577 SM00044 Adenylyl cyclase class-3/4/guanylyl cyclase

IPR001054 931 1139 SM00044 Adenylyl cyclase class-3/4/guanylyl cyclase

ProfileScan IPR001054 424 551 PS50125 Adenylyl cyclase class-3/4/guanylyl cyclase

IPR001054 971 1110 PS50125 Adenylyl cyclase class-3/4/guanylyl cyclase

ScanRegExp IPR001054 528 551 PS00452 Adenylyl cyclase class-3/4/guanylyl cyclase

IPR001054 1087 1110 PS00452 Adenylyl cyclase class-3/4/guanylyl cyclase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001054	415	599	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
IPR009398	605	714	PF06327	Adenylate cyclase-like
IPR001054	962	1156	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	378	577	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
IPR001054	931	1139	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	424	551	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
IPR001054	971	1110	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	528	551	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase
IPR001054	1087	1110	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 195 SSLTLLMAVLVLLTAVLLAFHA 216 PRIMARY 22

2 226 VALLACAAALFVGLMVVCNRHSF 248 PRIMARY 23

3 253 MWVVSYVVLGILAAVQVGGALAA 275 PRIMARY 23

4 284 LWCPVFFVYIAYTLLPIR 301 PRIMARY 18

5 333 QLGANVLLFLCTNVIGICTHYP 354 SECONDARY 22

6 713 RFGAYVACALLVFCFICFIQLLI 735 PRIMARY 23

7 744 GIYASIFLLLLITVLICAVYSCG 766 PRIMARY 23

8 794 SVLLVFTSAIANMYFIGNMLLSL 816 PRIMARY 23

9 832 AMIFVLGLIYLVLLLLGPPATIF 854 PRIMARY 23

10 886 LKYMTPVILLVFALALYLHAQQV 908 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	195	SSLTLLMAVLVLLTAVLLAFHA	216	PRIMARY	22
2	226	VALLACAAALFVGLMVVCNRHSF	248	PRIMARY	23
3	253	MWVVSYVVLGILAAVQVGGALAA	275	PRIMARY	23
4	284	LWCPVFFVYIAYTLLPIR	301	PRIMARY	18
5	333	QLGANVLLFLCTNVIGICTHYP	354	SECONDARY	22
6	713	RFGAYVACALLVFCFICFIQLLI	735	PRIMARY	23
7	744	GIYASIFLLLLITVLICAVYSCG	766	PRIMARY	23
8	794	SVLLVFTSAIANMYFIGNMLLSL	816	PRIMARY	23
9	832	AMIFVLGLIYLVLLLLGPPATIF	854	PRIMARY	23
10	886	LKYMTPVILLVFALALYLHAQQV	908	PRIMARY	23

Expression profile	Description

: RT-PCR Description

Experimental conditions

Primer_f
: ATGGGGTAGGGGTGAGGGTAT

Primer_r
: GGGTGAGGAAAGGGACAATGG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR	Description

RH mapping information	Description

Chromosome No.

: 12

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CAACTGGGTGAGGAAAGGGAC
Primer_r	: TGCTTCTTGCCAGGTGGGAGG
PCR product length	: 159 bp
PCR conditions	: 95 °C15 sec68 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	2393 bp
Genome contig ID	gi89161190r_47346248
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- ATATATTTAAATATCAATAAAATCAAACTCTTTGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAATTCTCTGTTCTCTGTAGCTGTTCTGGGCCCTGGGTGGGTCAGAAGT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	12	r	47446248	47469087	21	100.0	Perfect prediction

Primer_f	: ATGGGGTAGGGGTGAGGGTAT
Primer_r	: GGGTGAGGAAAGGGACAATGG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0422

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information