HUGE

Gene/Protein Characteristic Table for KIAA0199

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00453
Accession No. :	D83782
Description :	Sterol regulatory element-binding protein cleavage-activating protein.
HUGO Gene Name :	SREBF chaperone (SCAP)
Clone Name :	ha03150s1 [Vector Info]
Flexi ORF Clone :	pF1KSDA0199
Source :	Myeloblast cell line (KG-1)
Note :	We replaced ha03150, former representative clones for KIAA0199 with ha03150s1. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 4226 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 141 bp

Genome contig ID gi89161205r_47330207
PolyA signal sequence
(ATTAAA,-21)
+----*----+----*----+----*----+----
CTGACTGTAATAATATTAAACTTTTTTAAAAAACC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATATCATCATCTGTCAGGCACTTTGGGAGCTACTTGTGTCTCTTGGAATT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 3 r 47430207 47492439 23 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 1283 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q12770	100.0	Sterol regulato...	Homo sapiens
EAW64826	99.9	SREBP cleavage-...	Homo sapiens
XP_001499891	93.6	similar to Ster...	Equus caballus
A2RRU4	92.5	Sterol regulato...	Rattus norvegicus
A6QM06	92.5	Sterol regulato...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR001680 1096 1110 PR00320 WD40 repeat

IPR001680 1137 1151 PR00320 WD40 repeat

IPR001680 1177 1191 PR00320 WD40 repeat

HMMPfam IPR001680 770 806 PF00400 WD40 repeat

IPR001680 1073 1109 PF00400 WD40 repeat

IPR001680 1113 1150 PF00400 WD40 repeat

IPR001680 1154 1190 PF00400 WD40 repeat

HMMSmart IPR001680 769 806 SM00320 WD40 repeat

IPR001680 959 997 SM00320 WD40 repeat

IPR001680 1071 1109 SM00320 WD40 repeat

IPR001680 1112 1150 SM00320 WD40 repeat

IPR001680 1153 1190 SM00320 WD40 repeat

IPR001680 1193 1230 SM00320 WD40 repeat

ProfileScan IPR000731 288 446 PS50156 Sterol-sensing 5TM box

IPR001680 1079 1239 PS50294 WD40 repeat

IPR001680 1119 1159 PS50082 WD40 repeat

ScanRegExp IPR001680 1137 1151 PS00678 WD40 repeat

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001680	1096	1110	PR00320	WD40 repeat
IPR001680	1137	1151	PR00320	WD40 repeat
IPR001680	1177	1191	PR00320	WD40 repeat
HMMPfam	IPR001680	770	806	PF00400	WD40 repeat
IPR001680	1073	1109	PF00400	WD40 repeat
IPR001680	1113	1150	PF00400	WD40 repeat
IPR001680	1154	1190	PF00400	WD40 repeat
HMMSmart	IPR001680	769	806	SM00320	WD40 repeat
IPR001680	959	997	SM00320	WD40 repeat
IPR001680	1071	1109	SM00320	WD40 repeat
IPR001680	1112	1150	SM00320	WD40 repeat
IPR001680	1153	1190	SM00320	WD40 repeat
IPR001680	1193	1230	SM00320	WD40 repeat
ProfileScan	IPR000731	288	446	PS50156	Sterol-sensing 5TM box
IPR001680	1079	1239	PS50294	WD40 repeat
IPR001680	1119	1159	PS50082	WD40 repeat
ScanRegExp	IPR001680	1137	1151	PS00678	WD40 repeat

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 25 LCASYPIPIILFTGFCILACCYP 47 PRIMARY 23

2 284 IGVAELIPLVTTYIILFAYIYFS 306 PRIMARY 23

3 319 LALAAVVTVLSSLLMSVGLCTLF 341 PRIMARY 23

4 353 FPYLVVVIGLENVLVLTKSVVST 375 SECONDARY 23

5 401 ATELGIILIGYFTLVPAIQEFCL 423 PRIMARY 23

6 427 VGLVSDFFLQMLFFTTVLSIDI 448 SECONDARY 22

7 714 KVAALGLATGIVLVLLLLCLYRV 736 PRIMARY 23

8 776 LRGHLMDIECLASDGMLLVSCCL 798 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	LCASYPIPIILFTGFCILACCYP	47	PRIMARY	23
2	284	IGVAELIPLVTTYIILFAYIYFS	306	PRIMARY	23
3	319	LALAAVVTVLSSLLMSVGLCTLF	341	PRIMARY	23
4	353	FPYLVVVIGLENVLVLTKSVVST	375	SECONDARY	23
5	401	ATELGIILIGYFTLVPAIQEFCL	423	PRIMARY	23
6	427	VGLVSDFFLQMLFFTTVLSIDI	448	SECONDARY	22
7	714	KVAALGLATGIVLVLLLLCLYRV	736	PRIMARY	23
8	776	LRGHLMDIECLASDGMLLVSCCL	798	SECONDARY	23

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: 3

Experimental conditions

Panel name	: Genebridge 4
Primer_f	: GCCTGGTGTATGTGCCCTCTG
Primer_r	: CGGCTGGAAGATACTCGGCTC
PCR product length	: 140 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	141 bp
Genome contig ID	gi89161205r_47330207
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- CTGACTGTAATAATATTAAACTTTTTTAAAAAACC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATATCATCATCTGTCAGGCACTTTGGGAGCTACTTGTGTCTCTTGGAATT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	3	r	47430207	47492439	23	100.0	Perfect prediction

Gene/Protein Characteristic Table for KIAA0199

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information