|
Order Kazusa clone(s) from : |
| Product ID | ORK00958 |
|---|---|
| Accession No | AB075842 |
| Description | zinc finger protein 483, transcript variant 1 |
| Clone name | hm00158 |
| Vector information | |
| cDNA sequence | DNA sequence (3655 bp) Predicted protein sequence (746 aa) |
|
HaloTag ORF Clone |
FHC00958
|
| Flexi ORF Clone | FXC00958 |
| Source | Human adult brain |
Length: 3655 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 1262 bp |
|---|---|
| Genome contig ID | gi89161216f_113229366 |
| PolyA signal sequence (ACTAAA,-21) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (117169 - 117218) |
----+----*----+----*----+----*----+----*----+----* |
Ensembl ContigView (Add our DAS server as a DAS source)
| Chr | f/r | start | end | exon | identity | class | |
|---|---|---|---|---|---|---|---|
|
| 9 | f | 113327334 | 113346533 | 6 | 99.2 | Perfect prediction |
Length: 746 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| BlastProDom | IPR007087 | 441 | 464 | PD000003 | Zinc finger |
| IPR007087 | 469 | 492 | PD000003 | Zinc finger | |
| IPR007087 | 497 | 520 | PD000003 | Zinc finger | |
| IPR007087 | 525 | 548 | PD000003 | Zinc finger | |
| IPR007087 | 581 | 604 | PD000003 | Zinc finger | |
| IPR007087 | 637 | 660 | PD000003 | Zinc finger | |
| IPR007087 | 665 | 688 | PD000003 | Zinc finger | |
| IPR007087 | 693 | 716 | PD000003 | Zinc finger | |
| HMMPfam | IPR003309 | 48 | 142 | PF02023 | Transcriptional regulator SCAN |
| IPR001909 | 172 | 212 | PF01352 | KRAB box | |
| IPR007087 | 441 | 463 | PF00096 | Zinc finger | |
| IPR007087 | 469 | 491 | PF00096 | Zinc finger | |
| IPR007087 | 497 | 519 | PF00096 | Zinc finger | |
| IPR007087 | 525 | 547 | PF00096 | Zinc finger | |
| IPR007087 | 553 | 575 | PF00096 | Zinc finger | |
| IPR007087 | 581 | 603 | PF00096 | Zinc finger | |
| IPR007087 | 609 | 631 | PF00096 | Zinc finger | |
| IPR007087 | 637 | 659 | PF00096 | Zinc finger | |
| IPR007087 | 665 | 687 | PF00096 | Zinc finger | |
| IPR007087 | 693 | 715 | PF00096 | Zinc finger | |
| IPR007087 | 721 | 743 | PF00096 | Zinc finger | |
| HMMSmart | IPR003309 | 50 | 158 | SM00431 | Transcriptional regulator SCAN |
| IPR001909 | 172 | 232 | SM00349 | KRAB box | |
| IPR015880 | 441 | 463 | SM00355 | Zinc finger | |
| IPR015880 | 469 | 491 | SM00355 | Zinc finger | |
| IPR015880 | 497 | 519 | SM00355 | Zinc finger | |
| IPR015880 | 525 | 547 | SM00355 | Zinc finger | |
| IPR015880 | 553 | 575 | SM00355 | Zinc finger | |
| IPR015880 | 581 | 603 | SM00355 | Zinc finger | |
| IPR015880 | 609 | 631 | SM00355 | Zinc finger | |
| IPR015880 | 637 | 659 | SM00355 | Zinc finger | |
| IPR015880 | 665 | 687 | SM00355 | Zinc finger | |
| IPR015880 | 693 | 715 | SM00355 | Zinc finger | |
| IPR015880 | 721 | 743 | SM00355 | Zinc finger | |
| ProfileScan | IPR003309 | 54 | 136 | PS50804 | Transcriptional regulator SCAN |
| IPR001909 | 172 | 243 | PS50805 | KRAB box | |
| IPR007087 | 441 | 468 | PS50157 | Zinc finger | |
| IPR007087 | 469 | 496 | PS50157 | Zinc finger | |
| IPR007087 | 497 | 524 | PS50157 | Zinc finger | |
| IPR007087 | 525 | 552 | PS50157 | Zinc finger | |
| IPR007087 | 553 | 580 | PS50157 | Zinc finger | |
| IPR007087 | 581 | 608 | PS50157 | Zinc finger | |
| IPR007087 | 609 | 636 | PS50157 | Zinc finger | |
| IPR007087 | 637 | 664 | PS50157 | Zinc finger | |
| IPR007087 | 665 | 692 | PS50157 | Zinc finger | |
| IPR007087 | 693 | 720 | PS50157 | Zinc finger | |
| IPR007087 | 721 | 746 | PS50157 | Zinc finger | |
| ScanRegExp | IPR007087 | 443 | 463 | PS00028 | Zinc finger |
| IPR007087 | 471 | 491 | PS00028 | Zinc finger | |
| IPR007087 | 499 | 519 | PS00028 | Zinc finger | |
| IPR007087 | 527 | 547 | PS00028 | Zinc finger | |
| IPR007087 | 555 | 575 | PS00028 | Zinc finger | |
| IPR007087 | 583 | 603 | PS00028 | Zinc finger | |
| IPR007087 | 611 | 631 | PS00028 | Zinc finger | |
| IPR007087 | 639 | 659 | PS00028 | Zinc finger | |
| IPR007087 | 667 | 687 | PS00028 | Zinc finger | |
| IPR007087 | 695 | 715 | PS00028 | Zinc finger | |
| IPR007087 | 723 | 743 | PS00028 | Zinc finger |
RT-PCR-ELISA
|
Experimental conditions| Primer_f | TGGAAAACCTCAGGAACCTAG |
|---|---|
| Primer_r | AAAGCTGATTCATCTAGTCGG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 9
Experimental conditions| Panel name | genbank |
|---|---|
| Primer_f | - |
| Primer_r | - |
| PCR product length | - |
| PCR conditions | - |