##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII BamHI
BbeI BbrPI BbvCI BcgI BfrI
BglI BglII BlpI Bpu1102I BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstUI BstXI
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FauI
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MluI MroI
MspA1I MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 1812 ACATGT A^C[GA][CT]GT
AseI 2830 ATTAAT AT^TAAT
AsnI 2830 ATTAAT AT^TAAT
BalI 3310 TGGCCA TGG^CCA
BanI 910 GGTGCC G^G[CT][GA]CC
BciVI 2872 GTATCC GTATCC(6/5)
BcnI 1942 CCCGG CC^[GC]GG
BsaBI 921 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2298 ACCGGA [AT]^CCGG[AT]
BsgI 3199 GTGCAG GTGCAG(16/14)
BspLU11I 1812 ACATGT A^CATGT
BstAPI 27 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 527 GGTGACC G^GT[ACGT]ACC
BstPI 527 GGTGACC G^GT[ACGT]ACC
BstYI 569 AGATCC [GA]^GATC[CT]
CfoI 1510 GCGC GCG^C
DsaI 840 CCATGG C^C[GA][CT]GG
EaeI 3310 TGGCCA [CT]^GGCC[GA]
EcoO65I 527 GGTGACC G^GT[ACGT]ACC
HaeII 1509 AGCGCC [GA]GCGC^[CT]
HhaI 1510 GCGC GCG^C
HinP1I 1510 GCGC G^CGC
HincII 370 GTCAAC GT[CT]^[GA]AC
HindII 370 GTCAAC GT[CT]^[GA]AC
MamI 921 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 569 AGATCC [GA]^GATC[CT]
MluNI 3310 TGGCCA TGG^CCA
MscI 3310 TGGCCA TGG^CCA
NciI 1942 CCCGG CC^[GC]GG
NcoI 840 CCATGG C^CATGG
PacI 1107 TTAATTAA TTAAT^TAA
PshAI 2708 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2830 ATTAAT AT^TAAT
SacI 381 GAGCTC GAGCT^C
SspI 955 AATATT AAT^ATT
StuI 2354 AGGCCT AGG^CCT
XcmI 950 CCAGCAATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 569 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 764 CCTCAGG CC^T[ACGT]AGG
914 CCTCAGG
AvrII 3143 CCTAGG C^CTAGG
3375 CCTAGG
BanII 381 GAGCTC G[GA]GC[CT]^C
3071 GGGCTC
BbsI 2333 GAAGAC GAAGAC(2/6)
3416 GAAGAC
BclI 926 TGATCA T^GATCA
2473 TGATCA
BlnI 3143 CCTAGG C^CTAGG
3375 CCTAGG
BpuAI 2333 GAAGAC GAAGAC(2/6)
3416 GAAGAC
BsaI 401 GAGACC GGTCTC(1/5)
1745 GAGACC
BsmI 2604 GAATGC GAATGC(1/-1)
3172 GAATGC
Bsu36I 764 CCTCAGG CC^T[ACGT]AGG
914 CCTCAGG
Cac8I 161 GCAAGC GC[ACGT]^[ACGT]GC
3337 GCTTGC
DraI 2340 TTTAAA TTT^AAA
3462 TTTAAA
DraII 238 AGGTCCC [GA]G^G[ACGT]CC[CT]
1320 AGGTCCC
Eco81I 764 CCTCAGG CC^T[ACGT]AGG
914 CCTCAGG
EcoO109I 238 AGGTCCC [GA]G^G[ACGT]CC[CT]
1320 AGGTCCC
FbaI 926 TGATCA T^GATCA
2473 TGATCA
HindIII 1579 AAGCTT A^AGCTT
3645 AAGCTT
MaeII 1295 ACGT A^CGT
2016 ACGT
MfeI 1820 CAATTG C^AATTG
3500 CAATTG
MunI 1820 CAATTG C^AATTG
3500 CAATTG
NspI 1812 ACATGT [GA]CATG^[CT]
3569 GCATGT
PpuMI 238 AGGTCCC [GA]G^G[AT]CC[CT]
1320 AGGTCCC
TaqI 222 TCGA T^CGA
2261 TCGA
TthHB8I 222 TCGA T^CGA
2261 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
Bst1107I BstF5I BstNI BstZ17I Cfr13I
DdeI DpnII EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI