Gene/Protein Characteristic Table for KIAA0846
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Product ID ORK00656
Accession No AB020653
Description RAS guanyl releasing protein 3 (calcium and DAG-regulated), transcript variant 3
Clone name hk05386
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4204 bp)
Predicted protein sequence (691 aa)
Flexi ORF Clone FXC00656
Source Human adult brain
Rouge ID mKIAA0846 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4204 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1862 bp
Genome contig ID gi89161199f_33492446
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
AATAAACATTGTACTGTTCTTTTGCTTCTCAAAGG
Flanking genome sequence
(150732 - 150781)
----+----*----+----*----+----*----+----*----+----*
AATTATTCACTTGCCACTTTGGTTATTTTTGAGTTTTCGTACATAGGAAG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 f 33592446 33643176 17 99.6 Perfect prediction
Features of the protein sequence
Description

Length: 691 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAX00432 0 100.0 RAS guanyl rele...
Homo sapiens
Q8IV61 0 99.9 Ras guanyl-rele...
Homo sapiens
XP_001165459 0 99.9 RAS guanyl rele...
Pan troglodytes
BAF85672 0 99.6 unnamed protein...
Homo sapiens
XP_525730 0 99.7 RAS guanyl rele...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002349 8.8e-11 29.2 KIAA0351
D87467 2.3e-08 26.5 KIAA0277
AB028955 4e-08 47.7 KIAA1032
AB002311 2.5e-06 23.0 KIAA0313
AB023176 1.6e-05 27.0 KIAA0959
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR002219 493 507 PR00008 Protein kinase C
IPR002219 509 518 PR00008 Protein kinase C
IPR002219 522 533 PR00008 Protein kinase C
IPR002219 534 546 PR00008 Protein kinase C
HMMPfam IPR000651 2 104 PF00618 Guanine nucleotide exchange factor for Ras-like GTPases
IPR001895 148 334 PF00617 Guanine-nucleotide dissociation stimulator CDC25
IPR002048 425 453 PF00036 Calcium-binding EF-hand
IPR002048 454 482 PF00036 Calcium-binding EF-hand
IPR002219 496 548 PF00130 Protein kinase C
HMMSmart IPR000651 4 127 SM00229 Guanine nucleotide exchange factor for Ras-like GTPases
IPR001895 150 386 SM00147 Guanine-nucleotide dissociation stimulator CDC25
IPR002048 425 453 SM00054 Calcium-binding EF-hand
IPR002048 454 482 SM00054 Calcium-binding EF-hand
IPR002219 496 545 SM00109 Protein kinase C
ProfileScan IPR000651 5 127 PS50212 Guanine nucleotide exchange factor for Ras-like GTPases
IPR001895 154 385 PS50009 Guanine-nucleotide dissociation stimulator CDC25
IPR002048 421 456 PS50222 Calcium-binding EF-hand
IPR002048 459 485 PS50222 Calcium-binding EF-hand
IPR002219 495 545 PS50081 Protein kinase C
ScanRegExp IPR002048 434 446 PS00018 Calcium-binding EF-hand
IPR002048 463 475 PS00018 Calcium-binding EF-hand
IPR002219 496 545 PS00479 Protein kinase C
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f GTGCCAACCAGATTTACAGAT
Primer_r CACGTTCTCTTATTATCCAGC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name UniGene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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