Gene/Protein Characteristic Table for KIAA1070
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Product ID ORK00172
Accession No AB028993
Description neuroligin 1
Clone name hj05602
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4960 bp)
Predicted protein sequence (826 aa)
Flexi ORF Clone FXC00172
Source Human adult brain
Rouge ID mKIAA1070 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4960 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1819 bp
Genome contig ID gi89161205f_174685171
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
AAAATGTTTATTGCTGTTTTGTCTTTCTTTTTTCT
Flanking genome sequence
(798438 - 798487)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAGAAAAAAAAGAAAAAAAGGAAAAGAAGAACAAAGAAACATGAC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 3 f 174785171 175483607 6 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 826 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAH32555 0 100.0 Neuroligin 1 [H...
Homo sapiens
XP_001166321 0 99.9 neuroligin 1 is...
Pan troglodytes
XP_001082770 0 99.3 neuroligin 1 is...
Macaca mulatta
XP_001494442 0 99.1 neuroligin 1 is...
Equus caballus
XP_545297 0 98.9 similar to neur...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB037787 2.6e-111 63.0 KIAA1366
AB033086 2.1e-110 74.9 KIAA1260
AB023168 2.1e-110 75.0 KIAA0951
AB040913 1.4e-107 71.7 KIAA1480
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR000460 455 469 PR01090 Neuroligin
IPR000460 558 579 PR01090 Neuroligin
IPR000460 588 606 PR01090 Neuroligin
IPR000460 673 702 PR01090 Neuroligin
HMMPfam IPR002018 36 609 PF00135 Carboxylesterase
ScanRegExp IPR002018 154 164 PS00941 Carboxylesterase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 24 VHRGLGAPLTLCMLGCLLQAGHV 46 SECONDARY 23
2 679 LSVTIAVGASLLFLNILAFAAL 700 PRIMARY 22
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TTCATCCCCCTGTTGTTTACC
Primer_r TCAGGATGTGCTCTTTAAGTC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 3
Experimental conditions
Panel name GeneBridge 4
Primer_f TGAAGATGCTGCTCCAATACA
Primer_r TATACCTCCGATTTCTTACAG
PCR product length 106 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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