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Order Kazusa clone(s) from : |
| Product ID | ORK06162 |
|---|---|
| Accession No | AB040913 |
| Description | neuroligin 3 |
| Clone name | fj05645 |
| Vector information | |
| cDNA sequence | DNA sequence (3154 bp) Predicted protein sequence (682 aa) |
| Source | Human fetal brain |
| Rouge ID |
mKIAA1480
by Kazusa Mouse cDNA Project
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Length: 3154 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 1103 bp |
|---|---|
| Genome contig ID | gi89161218f_70190031 |
| PolyA signal sequence (AATAAA,-25) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (117745 - 117794) |
----+----*----+----*----+----*----+----*----+----* |
Length: 682 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR000460 | 304 | 318 | PR01090 | Neuroligin |
| IPR000460 | 407 | 428 | PR01090 | Neuroligin | |
| IPR000460 | 437 | 455 | PR01090 | Neuroligin | |
| IPR000460 | 536 | 565 | PR01090 | Neuroligin | |
| HMMPfam | IPR002018 | 1 | 458 | PF00135 | Carboxylesterase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 542 | LSVTIAVGASLLFLNVLAFAAL | 563 | PRIMARY | 22 |
|---|
RT-PCR-ELISA
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Experimental conditions| Primer_f | TGAACAACGTACTATGGAAGC |
|---|---|
| Primer_r | GACTGTAAAGAAAATGGGGCG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. X
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | TGAACAACGTACTATGGAAGC |
| Primer_r | GACTGTAAAGAAAATGGGGCG |
| PCR product length | 113 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |