Gene/Protein Characteristic Table for KIAA0946
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Product ID ORK00155
Accession No AB023163
Description zinc finger, DHHC-type containing 17
Clone name hj04787
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4715 bp)
Predicted protein sequence (667 aa)
Flexi ORF Clone FXC00155
Source Human adult brain
Rouge ID mKIAA0946 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4715 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 2709 bp
Genome contig ID gi89161190f_75582041
PolyA signal sequence
(AATAAA,-17)
+----*----+----*----+----*----+----
TTGTAAAATATTAATCAGAATAAATACTGACTCTT
Flanking genome sequence
(189566 - 189615)
----+----*----+----*----+----*----+----*----+----*
AAGTGTGTGCTTATGATTTCACTCAATTTACTTCAGGAAATTGATGAAAT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 12 f 75682041 75771605 17 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 667 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_509231 0 99.8 huntingtin inte...
Pan troglodytes
XP_001082309 0 99.7 huntingtin inte...
Macaca mulatta
Q8IUH5 0 100.0 Palmitoyltransf...
Homo sapiens
XP_614891 0 99.4 similar to hunt...
Bos taurus
BAC22089 0 99.7 huntingtin inte...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB051535 1.6e-10 30.6 KIAA1748
AB002377 3.2e-09 29.9 KIAA0379
AB046856 3.7e-09 29.5 KIAA1636
AB033076 5.7e-09 30.1 KIAA1250
AB095935 1.2e-08 27.1 KIAA2015
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR001594 431 504 PD003041 Zinc finger
FPrintScan IPR002110 159 171 PR01415 Ankyrin
IPR002110 272 284 PR01415 Ankyrin
HMMPfam IPR002110 124 156 PF00023 Ankyrin
IPR002110 158 190 PF00023 Ankyrin
IPR002110 191 223 PF00023 Ankyrin
IPR002110 224 257 PF00023 Ankyrin
IPR002110 259 291 PF00023 Ankyrin
IPR001594 468 527 PF01529 Zinc finger
HMMSmart IPR002110 124 153 SM00248 Ankyrin
IPR002110 158 187 SM00248 Ankyrin
IPR002110 191 220 SM00248 Ankyrin
IPR002110 224 254 SM00248 Ankyrin
IPR002110 259 288 SM00248 Ankyrin
ProfileScan IPR002110 99 312 PS50297 Ankyrin
IPR002110 124 156 PS50088 Ankyrin
IPR002110 158 190 PS50088 Ankyrin
IPR002110 191 223 PS50088 Ankyrin
IPR002110 224 257 PS50088 Ankyrin
IPR002110 259 291 PS50088 Ankyrin
IPR001594 472 522 PS50216 Zinc finger

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 339 KVMLGTPFLVIWLVGFIADLNID 361 PRIMARY 23
2 363 WLIKGLMYGGVWATVQFLSKSFF 385 SECONDARY 23
3 392 ALPLGIYLATKFWMYVTWFFWFW 414 SECONDARY 23
4 417 LNFLFIHLPFLANSVALFYNFGK 439 SECONDARY 23
5 515 RYFMGYLFFLLFMICWMIYGCIS 537 PRIMARY 23
6 571 LNSVFHFMWVAVLLMCQMYQISC 593 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f AAATGGTCAGATGGTCAGGAG
Primer_r CCCAAAGGTTCACCATCATAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 12
Experimental conditions
Panel name UniGene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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