Gene/Protein Characteristic Table for KIAA1142
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Product ID ORK00749
Accession No AB032968
Description p21 protein (Cdc42/Rac)-activated kinase 4, transcript variant 5
Clone name hh05864
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5742 bp)
Predicted protein sequence (467 aa)
Flexi ORF Clone FXC00749
Source Human adult brain
Rouge ID mKIAA1142 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5742 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 4244 bp
Genome contig ID gi42406306f_44208314
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
GCACTCCAGCCTGGGTGACAGAGTGAGATTCCGTC
Flanking genome sequence
(156984 - 157033)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAGACAAAAAACAACAGGTACGGTGGCTTACACCTGTAA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 19 f 44308314 44365296 9 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 467 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
CAD38914 9.7e-126 100.0 hypothetical pr...
Homo sapiens
XP_867241 5.7e-121 95.9 similar to p21-...
Canis lupus fam...
EAW56862 4.7e-108 89.1 p21(CDKN1A)-act...
Homo sapiens
AAH34511 3.5e-105 87.4 PAK4 protein [H...
Homo sapiens
BAG56999 1.1e-104 95.4 unnamed protein...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB033090 9e-43 65.6 KIAA1264
D86959 1.3e-12 33.6 KIAA0204
AB020688 3.9e-12 35.9 KIAA0881
AB037782 7e-12 37.0 KIAA1361
AB011123 4.3e-11 38.6 KIAA0551
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR000719 203 447 PD000001 Protein kinase
HMMPfam IPR000095 39 96 PF00786 PAK-box/P21-Rho-binding
IPR000719 197 448 PF00069 Protein kinase
HMMSmart IPR000095 40 75 SM00285 PAK-box/P21-Rho-binding
IPR001245 197 448 SM00219 Tyrosine protein kinase
IPR002290 197 448 SM00220 Serine/threonine protein kinase
ProfileScan IPR000095 40 53 PS50108 PAK-box/P21-Rho-binding
IPR000719 197 448 PS50011 Protein kinase
ScanRegExp IPR000719 203 227 PS00107 Protein kinase
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f AGGTCATAGCAGCCAGTAAGC
Primer_r TACTCACCAAACGCGCTCAAG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 19
Experimental conditions
Panel name unigene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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